Mutagenetix > Incidental Mutation

Incidental Mutation 'R0098:Prune2'

216100

Institutional Source

Beutler Lab

Gene Symbol

Prune2

Ensembl Gene

ENSMUSG00000039126

Gene Name

prune homolog 2

Synonyms

A230083H22Rik, A330102H22Rik, 6330414G02Rik, Olfaxin

MMRRC Submission

038384-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0098 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

16933482-17201296 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 17101267 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 2257 (E2257G)

Ref Sequence

ENSEMBL: ENSMUSP00000084977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087689]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000087689
AA Change: E2257G

PolyPhen 2 Score 0.501 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000084977
Gene: ENSMUSG00000039126
AA Change: E2257G

Domain	Start	End	E-Value	Type
DHHA2	208	351	8.32e-17	SMART
low complexity region	433	445	N/A	INTRINSIC
low complexity region	476	488	N/A	INTRINSIC
low complexity region	547	553	N/A	INTRINSIC
low complexity region	962	975	N/A	INTRINSIC
low complexity region	1071	1082	N/A	INTRINSIC
low complexity region	1368	1378	N/A	INTRINSIC
low complexity region	1533	1545	N/A	INTRINSIC
low complexity region	1668	1685	N/A	INTRINSIC
low complexity region	1740	1751	N/A	INTRINSIC
low complexity region	2162	2175	N/A	INTRINSIC
low complexity region	2222	2233	N/A	INTRINSIC
low complexity region	2591	2606	N/A	INTRINSIC
low complexity region	2731	2744	N/A	INTRINSIC
SEC14	2882	3037	2.08e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000226052

Meta Mutation Damage Score

0.0863

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.8%
20x: 96.2%

Validation Efficiency

100% (54/54)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Allele List at MGI

All alleles(160) : Gene trapped(160)

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad9	T	C	3: 36,127,689 (GRCm39)	I97T	probably damaging	Het
Acp3	C	T	9: 104,197,144 (GRCm39)		probably null	Het
Adam32	T	A	8: 25,404,405 (GRCm39)	Y200F	possibly damaging	Het
Alpk2	A	G	18: 65,482,982 (GRCm39)	L342S	probably damaging	Het
Arfgef3	A	G	10: 18,465,390 (GRCm39)	V2151A	probably damaging	Het
Atm	T	C	9: 53,429,869 (GRCm39)	D389G	probably benign	Het
Atp10b	A	T	11: 43,080,431 (GRCm39)	S236C	probably benign	Het
B3gat1	C	T	9: 26,668,237 (GRCm39)	R276C	probably damaging	Het
Cndp1	T	A	18: 84,646,949 (GRCm39)	E246D	probably damaging	Het
Crebbp	A	G	16: 3,909,792 (GRCm39)	L1078P	probably damaging	Het
Cyp20a1	G	T	1: 60,426,413 (GRCm39)	E452*	probably null	Het
Emb	T	C	13: 117,404,034 (GRCm39)	V262A	probably damaging	Het
Ephb1	C	T	9: 101,918,339 (GRCm39)	R390H	probably damaging	Het
Faf1	T	C	4: 109,792,696 (GRCm39)	L556S	probably damaging	Het
Fam237b	T	A	5: 5,625,355 (GRCm39)	L17Q	possibly damaging	Het
Fbf1	A	T	11: 116,038,945 (GRCm39)		probably null	Het
Gid8	T	A	2: 180,356,528 (GRCm39)	I55N	possibly damaging	Het
Hexa	T	C	9: 59,465,383 (GRCm39)	Y213H	probably damaging	Het
Kalrn	A	T	16: 33,795,989 (GRCm39)	I1262K	possibly damaging	Het
Lrp1	C	T	10: 127,388,607 (GRCm39)	V3281I	probably benign	Het
Lrp2	T	C	2: 69,305,756 (GRCm39)	D2935G	probably damaging	Het
Lypd6	T	A	2: 50,080,792 (GRCm39)	V160E	probably benign	Het
Muc19	C	T	15: 91,777,101 (GRCm39)		noncoding transcript	Het
Mybpc1	T	C	10: 88,365,426 (GRCm39)	D899G	probably benign	Het
Myo18a	A	G	11: 77,736,591 (GRCm39)	E1564G	probably damaging	Het
Nrxn3	A	G	12: 89,226,971 (GRCm39)	D202G	probably damaging	Het
Palld	C	A	8: 61,978,120 (GRCm39)	G890V	probably damaging	Het
Pcx	C	A	19: 4,651,775 (GRCm39)		probably benign	Het
Plcg1	T	C	2: 160,573,920 (GRCm39)	W62R	probably damaging	Het
Ppa2	C	T	3: 133,076,234 (GRCm39)		probably benign	Het
Ppp1r18	A	G	17: 36,178,888 (GRCm39)	I254M	probably benign	Het
Rd3	A	G	1: 191,717,261 (GRCm39)	M244V	probably benign	Het
Rfx5	T	A	3: 94,865,679 (GRCm39)	V326E	probably damaging	Het
Rgs3	G	C	4: 62,544,143 (GRCm39)	R305P	probably damaging	Het
Rpp40	A	G	13: 36,082,970 (GRCm39)	Y173H	probably benign	Het
Ryr3	T	C	2: 112,731,376 (GRCm39)	N645D	probably damaging	Het
Sema3e	T	C	5: 14,302,446 (GRCm39)	V657A	possibly damaging	Het
Serpina3n	T	A	12: 104,379,777 (GRCm39)	V390E	probably damaging	Het
Shank1	A	G	7: 43,962,709 (GRCm39)	Y141C	unknown	Het
Stat2	T	A	10: 128,119,131 (GRCm39)	H428Q	probably damaging	Het
Stat5a	A	T	11: 100,766,452 (GRCm39)	Q378L	probably damaging	Het
Tfrc	G	T	16: 32,442,244 (GRCm39)	V490F	probably damaging	Het
Tnnt1	T	C	7: 4,512,044 (GRCm39)	N155S	probably damaging	Het
Topaz1	T	C	9: 122,619,188 (GRCm39)	Y1262H	possibly damaging	Het
Ubxn8	T	C	8: 34,125,393 (GRCm39)		probably benign	Het
Unk	A	G	11: 115,940,995 (GRCm39)	Y252C	probably damaging	Het
Vmn2r66	A	C	7: 84,654,965 (GRCm39)	M448R	probably damaging	Het
Zfp386	T	A	12: 116,022,834 (GRCm39)	L184*	probably null	Het
Zfp985	T	C	4: 147,661,566 (GRCm39)	S4P	probably damaging	Het

Other mutations in Prune2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00234:Prune2	APN	19	17,145,708 (GRCm39)	critical splice donor site	probably null
IGL00848:Prune2	APN	19	17,096,482 (GRCm39)	missense	probably damaging	1.00
IGL00862:Prune2	APN	19	17,096,713 (GRCm39)	missense	probably benign	0.41
IGL00915:Prune2	APN	19	16,993,617 (GRCm39)	missense	probably damaging	1.00
IGL01084:Prune2	APN	19	17,095,573 (GRCm39)	missense	probably benign	0.19
IGL01109:Prune2	APN	19	17,101,243 (GRCm39)	missense	probably benign	0.03
IGL01372:Prune2	APN	19	17,102,433 (GRCm39)	missense	probably damaging	1.00
IGL01650:Prune2	APN	19	17,145,656 (GRCm39)	missense	possibly damaging	0.95
IGL01752:Prune2	APN	19	17,101,267 (GRCm39)	missense	possibly damaging	0.50
IGL01812:Prune2	APN	19	16,981,141 (GRCm39)	missense	possibly damaging	0.50
IGL01902:Prune2	APN	19	17,096,002 (GRCm39)	missense	probably benign	0.00
IGL02195:Prune2	APN	19	17,096,921 (GRCm39)	missense	probably benign	0.00
IGL02502:Prune2	APN	19	17,101,245 (GRCm39)	missense	probably benign	0.00
IGL02569:Prune2	APN	19	17,156,223 (GRCm39)	missense	probably damaging	0.99
IGL02693:Prune2	APN	19	17,101,855 (GRCm39)	missense	probably benign	0.03
IGL02737:Prune2	APN	19	17,170,775 (GRCm39)	nonsense	probably null
IGL02794:Prune2	APN	19	17,096,725 (GRCm39)	missense	probably benign	0.19
IGL02985:Prune2	APN	19	16,993,723 (GRCm39)	critical splice donor site	probably null
IGL03349:Prune2	APN	19	17,100,710 (GRCm39)	missense	probably damaging	1.00
3-1:Prune2	UTSW	19	17,102,646 (GRCm39)	missense	probably benign	0.00
R0060:Prune2	UTSW	19	16,981,097 (GRCm39)	missense	probably damaging	1.00
R0098:Prune2	UTSW	19	17,101,267 (GRCm39)	missense	possibly damaging	0.50
R0165:Prune2	UTSW	19	17,099,974 (GRCm39)	missense	probably benign	0.00
R0277:Prune2	UTSW	19	17,098,753 (GRCm39)	missense	probably damaging	0.99
R0321:Prune2	UTSW	19	17,099,818 (GRCm39)	missense	probably benign	0.39
R0321:Prune2	UTSW	19	17,098,291 (GRCm39)	missense	possibly damaging	0.78
R0374:Prune2	UTSW	19	17,098,274 (GRCm39)	missense	probably benign	0.00
R0380:Prune2	UTSW	19	17,101,371 (GRCm39)	missense	probably damaging	1.00
R0396:Prune2	UTSW	19	17,100,444 (GRCm39)	missense	probably benign	0.35
R0408:Prune2	UTSW	19	17,099,674 (GRCm39)	missense	probably benign	0.00
R0421:Prune2	UTSW	19	17,100,675 (GRCm39)	missense	probably benign	0.02
R0480:Prune2	UTSW	19	16,984,156 (GRCm39)	splice site	probably benign
R0531:Prune2	UTSW	19	16,984,117 (GRCm39)	missense	probably damaging	1.00
R0546:Prune2	UTSW	19	16,998,030 (GRCm39)	splice site	probably benign
R0554:Prune2	UTSW	19	17,102,582 (GRCm39)	nonsense	probably null
R0659:Prune2	UTSW	19	17,100,199 (GRCm39)	missense	probably damaging	1.00
R0699:Prune2	UTSW	19	17,101,319 (GRCm39)	missense	probably damaging	1.00
R0781:Prune2	UTSW	19	17,102,586 (GRCm39)	missense	probably benign
R1110:Prune2	UTSW	19	17,102,586 (GRCm39)	missense	probably benign
R1178:Prune2	UTSW	19	17,100,469 (GRCm39)	missense	probably benign	0.22
R1181:Prune2	UTSW	19	17,100,469 (GRCm39)	missense	probably benign	0.22
R1337:Prune2	UTSW	19	17,096,971 (GRCm39)	missense	possibly damaging	0.70
R1356:Prune2	UTSW	19	17,189,681 (GRCm39)	missense	probably benign	0.40
R1385:Prune2	UTSW	19	17,102,312 (GRCm39)	missense	possibly damaging	0.50
R1659:Prune2	UTSW	19	17,098,015 (GRCm39)	missense	possibly damaging	0.59
R1738:Prune2	UTSW	19	17,102,374 (GRCm39)	missense	probably benign	0.01
R1756:Prune2	UTSW	19	17,101,068 (GRCm39)	missense	probably benign	0.01
R1765:Prune2	UTSW	19	17,102,962 (GRCm39)	missense	probably damaging	1.00
R1782:Prune2	UTSW	19	17,099,537 (GRCm39)	missense	probably benign	0.00
R1817:Prune2	UTSW	19	17,099,445 (GRCm39)	missense	probably benign	0.00
R1838:Prune2	UTSW	19	17,177,242 (GRCm39)	missense	probably damaging	1.00
R1851:Prune2	UTSW	19	17,176,503 (GRCm39)	missense	probably damaging	1.00
R1852:Prune2	UTSW	19	17,176,503 (GRCm39)	missense	probably damaging	1.00
R1866:Prune2	UTSW	19	17,100,856 (GRCm39)	missense	probably damaging	1.00
R1911:Prune2	UTSW	19	17,091,038 (GRCm39)	missense	probably benign	0.02
R1983:Prune2	UTSW	19	16,998,006 (GRCm39)	missense	probably damaging	0.97
R2014:Prune2	UTSW	19	17,097,887 (GRCm39)	missense	probably damaging	1.00
R2066:Prune2	UTSW	19	17,098,042 (GRCm39)	missense	possibly damaging	0.57
R2088:Prune2	UTSW	19	17,097,109 (GRCm39)	missense	possibly damaging	0.95
R2111:Prune2	UTSW	19	17,185,602 (GRCm39)	missense	probably damaging	1.00
R2128:Prune2	UTSW	19	17,099,786 (GRCm39)	missense	probably benign	0.00
R2165:Prune2	UTSW	19	17,097,546 (GRCm39)	missense	probably benign	0.19
R2241:Prune2	UTSW	19	17,100,456 (GRCm39)	missense	probably damaging	0.96
R2278:Prune2	UTSW	19	17,095,919 (GRCm39)	missense	possibly damaging	0.93
R2504:Prune2	UTSW	19	16,977,400 (GRCm39)	missense	probably damaging	1.00
R2508:Prune2	UTSW	19	17,099,986 (GRCm39)	missense	probably benign	0.43
R3055:Prune2	UTSW	19	17,102,407 (GRCm39)	missense	probably damaging	0.98
R3086:Prune2	UTSW	19	17,098,777 (GRCm39)	missense	possibly damaging	0.75
R3104:Prune2	UTSW	19	17,096,520 (GRCm39)	missense	probably damaging	1.00
R3105:Prune2	UTSW	19	17,096,520 (GRCm39)	missense	probably damaging	1.00
R3547:Prune2	UTSW	19	17,101,712 (GRCm39)	missense	probably damaging	0.96
R3702:Prune2	UTSW	19	17,156,235 (GRCm39)	missense	probably damaging	1.00
R3753:Prune2	UTSW	19	17,102,818 (GRCm39)	missense	probably benign	0.38
R3933:Prune2	UTSW	19	17,101,318 (GRCm39)	missense	probably damaging	1.00
R3935:Prune2	UTSW	19	17,177,150 (GRCm39)	missense	probably damaging	1.00
R4022:Prune2	UTSW	19	16,977,384 (GRCm39)	missense	probably damaging	1.00
R4042:Prune2	UTSW	19	16,981,190 (GRCm39)	critical splice donor site	probably null
R4164:Prune2	UTSW	19	16,981,098 (GRCm39)	missense	possibly damaging	0.87
R4453:Prune2	UTSW	19	17,099,274 (GRCm39)	missense	probably benign	0.00
R4642:Prune2	UTSW	19	16,998,019 (GRCm39)	critical splice donor site	probably null
R4661:Prune2	UTSW	19	16,977,387 (GRCm39)	missense	probably damaging	1.00
R4666:Prune2	UTSW	19	17,097,552 (GRCm39)	nonsense	probably null
R4823:Prune2	UTSW	19	17,097,868 (GRCm39)	missense	probably damaging	1.00
R4897:Prune2	UTSW	19	17,099,219 (GRCm39)	missense	probably benign	0.03
R4922:Prune2	UTSW	19	17,100,116 (GRCm39)	missense	probably benign	0.00
R4962:Prune2	UTSW	19	17,099,637 (GRCm39)	missense	probably benign	0.11
R5026:Prune2	UTSW	19	17,176,506 (GRCm39)	missense	probably damaging	1.00
R5042:Prune2	UTSW	19	17,097,161 (GRCm39)	missense	possibly damaging	0.94
R5124:Prune2	UTSW	19	17,177,274 (GRCm39)	missense	probably damaging	1.00
R5133:Prune2	UTSW	19	16,980,995 (GRCm39)	missense	probably damaging	1.00
R5184:Prune2	UTSW	19	17,193,721 (GRCm39)	missense	possibly damaging	0.95
R5234:Prune2	UTSW	19	17,096,032 (GRCm39)	missense	probably damaging	1.00
R5339:Prune2	UTSW	19	17,098,236 (GRCm39)	missense	probably damaging	1.00
R5363:Prune2	UTSW	19	17,095,630 (GRCm39)	missense	probably damaging	1.00
R5382:Prune2	UTSW	19	16,981,023 (GRCm39)	missense	probably damaging	1.00
R5436:Prune2	UTSW	19	16,998,007 (GRCm39)	missense	probably damaging	1.00
R5480:Prune2	UTSW	19	17,098,311 (GRCm39)	missense	possibly damaging	0.66
R5635:Prune2	UTSW	19	17,095,573 (GRCm39)	missense	probably benign	0.19
R5678:Prune2	UTSW	19	17,096,032 (GRCm39)	missense	probably damaging	1.00
R5814:Prune2	UTSW	19	16,993,725 (GRCm39)	splice site	probably null
R5894:Prune2	UTSW	19	17,098,755 (GRCm39)	missense	possibly damaging	0.88
R6011:Prune2	UTSW	19	17,096,080 (GRCm39)	missense	probably benign	0.35
R6207:Prune2	UTSW	19	17,095,480 (GRCm39)	missense	probably damaging	1.00
R6218:Prune2	UTSW	19	17,098,926 (GRCm39)	missense	probably benign	0.00
R6573:Prune2	UTSW	19	17,098,522 (GRCm39)	missense	possibly damaging	0.61
R6573:Prune2	UTSW	19	17,098,521 (GRCm39)	missense	probably damaging	1.00
R6734:Prune2	UTSW	19	16,981,097 (GRCm39)	missense	probably damaging	1.00
R6805:Prune2	UTSW	19	17,097,954 (GRCm39)	missense	probably benign
R6837:Prune2	UTSW	19	17,156,292 (GRCm39)	missense	probably damaging	1.00
R6850:Prune2	UTSW	19	17,099,552 (GRCm39)	missense	probably benign	0.00
R6858:Prune2	UTSW	19	17,095,470 (GRCm39)	missense	possibly damaging	0.70
R6874:Prune2	UTSW	19	17,100,592 (GRCm39)	missense	probably damaging	1.00
R6954:Prune2	UTSW	19	16,977,385 (GRCm39)	missense	probably damaging	1.00
R7098:Prune2	UTSW	19	17,097,966 (GRCm39)	missense	probably benign	0.39
R7102:Prune2	UTSW	19	17,098,577 (GRCm39)	missense	probably benign	0.24
R7246:Prune2	UTSW	19	17,098,732 (GRCm39)	missense	probably damaging	0.99
R7284:Prune2	UTSW	19	17,097,250 (GRCm39)	missense	probably damaging	1.00
R7295:Prune2	UTSW	19	17,097,261 (GRCm39)	missense	probably benign	0.01
R7371:Prune2	UTSW	19	17,096,734 (GRCm39)	missense	probably benign	0.02
R7651:Prune2	UTSW	19	17,097,772 (GRCm39)	missense	probably damaging	1.00
R7830:Prune2	UTSW	19	17,100,038 (GRCm39)	missense	probably benign	0.21
R7872:Prune2	UTSW	19	17,096,798 (GRCm39)	missense	probably benign	0.05
R7881:Prune2	UTSW	19	17,100,393 (GRCm39)	missense	possibly damaging	0.50
R7966:Prune2	UTSW	19	17,156,223 (GRCm39)	missense	probably damaging	0.99
R7969:Prune2	UTSW	19	17,179,034 (GRCm39)	missense	probably damaging	0.98
R8092:Prune2	UTSW	19	17,097,357 (GRCm39)	missense	probably damaging	1.00
R8110:Prune2	UTSW	19	17,098,083 (GRCm39)	missense	probably benign	0.22
R8115:Prune2	UTSW	19	17,101,288 (GRCm39)	missense	probably benign	0.02
R8129:Prune2	UTSW	19	17,096,200 (GRCm39)	missense	probably benign	0.01
R8169:Prune2	UTSW	19	17,102,455 (GRCm39)	missense	probably benign	0.10
R8171:Prune2	UTSW	19	17,097,882 (GRCm39)	missense	probably damaging	1.00
R8176:Prune2	UTSW	19	17,095,656 (GRCm39)	missense	probably damaging	1.00
R8200:Prune2	UTSW	19	17,102,337 (GRCm39)	missense	probably benign	0.01
R8217:Prune2	UTSW	19	17,097,480 (GRCm39)	missense	probably benign	0.01
R8258:Prune2	UTSW	19	17,189,672 (GRCm39)	missense	unknown
R8259:Prune2	UTSW	19	17,189,672 (GRCm39)	missense	unknown
R8289:Prune2	UTSW	19	17,100,373 (GRCm39)	missense	probably benign	0.43
R8329:Prune2	UTSW	19	17,098,629 (GRCm39)	missense	probably benign	0.02
R8342:Prune2	UTSW	19	17,103,027 (GRCm39)	missense	probably benign	0.01
R8558:Prune2	UTSW	19	17,099,602 (GRCm39)	missense	probably damaging	0.98
R8732:Prune2	UTSW	19	17,097,769 (GRCm39)	missense	probably damaging	1.00
R8743:Prune2	UTSW	19	17,096,920 (GRCm39)	missense	probably benign	0.22
R8769:Prune2	UTSW	19	17,100,442 (GRCm39)	missense	probably damaging	0.96
R8862:Prune2	UTSW	19	17,097,510 (GRCm39)	missense	probably benign	0.04
R8936:Prune2	UTSW	19	17,099,199 (GRCm39)	missense	probably benign	0.24
R9040:Prune2	UTSW	19	17,097,991 (GRCm39)	missense	probably damaging	1.00
R9084:Prune2	UTSW	19	17,097,741 (GRCm39)	missense	probably damaging	1.00
R9224:Prune2	UTSW	19	17,097,393 (GRCm39)	missense	probably damaging	1.00
R9273:Prune2	UTSW	19	17,095,690 (GRCm39)	missense	possibly damaging	0.74
R9275:Prune2	UTSW	19	17,101,144 (GRCm39)	missense	probably benign	0.06
R9278:Prune2	UTSW	19	17,101,144 (GRCm39)	missense	probably benign	0.06
R9290:Prune2	UTSW	19	17,145,691 (GRCm39)	missense	probably benign	0.41
R9305:Prune2	UTSW	19	17,097,625 (GRCm39)	missense	probably benign	0.14
R9317:Prune2	UTSW	19	17,099,034 (GRCm39)	missense	probably benign	0.00
R9354:Prune2	UTSW	19	17,099,986 (GRCm39)	missense	probably benign	0.43
R9373:Prune2	UTSW	19	17,099,502 (GRCm39)	missense	probably benign
R9394:Prune2	UTSW	19	16,981,053 (GRCm39)	missense	probably damaging	1.00
R9405:Prune2	UTSW	19	17,193,708 (GRCm39)	missense	probably damaging	0.99
R9476:Prune2	UTSW	19	17,096,706 (GRCm39)	missense	possibly damaging	0.64
R9532:Prune2	UTSW	19	17,099,794 (GRCm39)	missense	probably benign	0.00
X0019:Prune2	UTSW	19	17,098,881 (GRCm39)	missense	probably benign	0.16
X0028:Prune2	UTSW	19	17,100,249 (GRCm39)	missense	probably damaging	1.00
X0064:Prune2	UTSW	19	17,099,739 (GRCm39)	missense	probably damaging	1.00
X0066:Prune2	UTSW	19	17,096,154 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCCCCAGAAGTGAAGACGCTGAC -3'
(R):5'- TTCCTCTGACGGAAGATCACCACC -3'

Sequencing Primer
(F):5'- GACGCTGACACTTTTGAAGC -3'
(R):5'- GCTTCAGATGAACAGACATCTTGC -3'

Posted On

2014-07-21