Mutagenetix > Incidental Mutation

Incidental Mutation 'R1962:Vmn2r27'

216964

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

039976-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R1962 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

124168555-124208743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 124200793 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 388 (R388Q)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100968
AA Change: R388Q

PolyPhen 2 Score 0.698 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: R388Q

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	A	G	7: 119,940,468 (GRCm39)	I354M	probably damaging	Het
Abca8a	T	C	11: 109,917,731 (GRCm39)		probably null	Het
Abca8b	T	C	11: 109,870,724 (GRCm39)	R143G	probably benign	Het
Actn4	T	C	7: 28,594,047 (GRCm39)	D840G	probably damaging	Het
Agpat5	T	C	8: 18,928,026 (GRCm39)	L197P	probably damaging	Het
Akr1d1	T	C	6: 37,512,983 (GRCm39)	V93A	probably benign	Het
Arap2	T	C	5: 62,834,007 (GRCm39)	K820R	possibly damaging	Het
Armc9	T	A	1: 86,135,696 (GRCm39)	C551S	probably damaging	Het
Atg7	T	C	6: 114,683,191 (GRCm39)	L418P	probably damaging	Het
Awat2	G	A	X: 99,448,165 (GRCm39)	P148S	probably damaging	Het
Brms1	C	T	19: 5,096,027 (GRCm39)	R34W	probably damaging	Het
Cbx2	A	G	11: 118,919,395 (GRCm39)	Q320R	possibly damaging	Het
Ccdc106	G	A	7: 5,062,539 (GRCm39)	D11N	possibly damaging	Het
Ccdc30	C	T	4: 119,196,988 (GRCm39)	R426Q	probably benign	Het
Cdc42bpg	T	A	19: 6,356,885 (GRCm39)	V47E	probably damaging	Het
Cep170b	C	T	12: 112,704,495 (GRCm39)	S751L	probably damaging	Het
Cfap46	A	T	7: 139,246,957 (GRCm39)	L328Q	probably damaging	Het
Crtc3	A	G	7: 80,239,679 (GRCm39)	F558L	probably damaging	Het
Cyp2d34	A	G	15: 82,502,809 (GRCm39)	V139A	probably benign	Het
Dchs1	A	G	7: 105,413,408 (GRCm39)	Y1136H	probably damaging	Het
Dhrs4	T	C	14: 55,725,060 (GRCm39)	V185A	probably damaging	Het
Dnah7b	A	G	1: 46,281,263 (GRCm39)	K2775E	possibly damaging	Het
Dst	C	A	1: 34,230,097 (GRCm39)	S2238R	possibly damaging	Het
Duox2	T	C	2: 122,127,853 (GRCm39)		probably null	Het
Dusp16	G	T	6: 134,695,099 (GRCm39)	Y577*	probably null	Het
Dync1h1	G	A	12: 110,602,943 (GRCm39)	E2195K	probably benign	Het
Eml5	A	G	12: 98,842,570 (GRCm39)	F176S	probably damaging	Het
Esco2	C	A	14: 66,068,982 (GRCm39)	R109S	probably damaging	Het
Galnt7	C	T	8: 57,985,748 (GRCm39)	E541K	probably benign	Het
Gbf1	C	T	19: 46,255,658 (GRCm39)	T707I	probably damaging	Het
Gdf5	A	G	2: 155,783,672 (GRCm39)	C427R	probably damaging	Het
Glyctk	T	C	9: 106,035,064 (GRCm39)	M1V	probably null	Het
Gm5478	T	C	15: 101,552,830 (GRCm39)	E367G	probably damaging	Het
Golga7b	G	A	19: 42,251,768 (GRCm39)	V5I	probably benign	Het
Gpt2	T	C	8: 86,219,764 (GRCm39)	L70P	probably damaging	Het
Gsdmc3	C	A	15: 63,730,315 (GRCm39)	Q416H	probably damaging	Het
Hoxb5	A	G	11: 96,194,918 (GRCm39)	E160G	probably benign	Het
Ift81	A	T	5: 122,698,772 (GRCm39)	Y532N	probably benign	Het
Igf1	G	A	10: 87,700,726 (GRCm39)	C66Y	probably damaging	Het
Igf1r	T	C	7: 67,857,023 (GRCm39)	V995A	probably damaging	Het
Ip6k1	T	C	9: 107,918,287 (GRCm39)		probably null	Het
Jaml	T	C	9: 45,015,495 (GRCm39)	I333T	possibly damaging	Het
Kdm4c	T	C	4: 74,225,253 (GRCm39)		probably benign	Het
Kdm6b	T	C	11: 69,292,191 (GRCm39)		probably benign	Het
Krt6a	C	T	15: 101,599,900 (GRCm39)	R404H	probably damaging	Het
Larp4b	C	A	13: 9,186,878 (GRCm39)	H69N	probably benign	Het
Lcat	A	T	8: 106,668,355 (GRCm39)	W222R	probably damaging	Het
Lrrc40	T	A	3: 157,746,086 (GRCm39)	C54S	probably benign	Het
Mcpt9	T	A	14: 56,265,024 (GRCm39)	H159L	probably benign	Het
Megf8	T	C	7: 25,062,976 (GRCm39)	V2444A	probably damaging	Het
Memo1	T	C	17: 74,552,003 (GRCm39)	T98A	possibly damaging	Het
Mical2	A	T	7: 112,012,051 (GRCm39)	I634L	probably benign	Het
Mov10	C	T	3: 104,704,293 (GRCm39)	R835Q	probably damaging	Het
Mybpc1	A	T	10: 88,384,688 (GRCm39)	L546Q	probably damaging	Het
Myo6	T	C	9: 80,168,117 (GRCm39)	V427A	probably damaging	Het
Myom2	T	A	8: 15,182,599 (GRCm39)		probably null	Het
Mzt2	G	A	16: 15,666,543 (GRCm39)	R125C	probably damaging	Het
Neb	T	A	2: 52,162,949 (GRCm39)	R2031*	probably null	Het
Nphp3	T	C	9: 103,898,537 (GRCm39)	S447P	probably benign	Het
Nrp2	T	A	1: 62,758,090 (GRCm39)	D25E	probably benign	Het
Nts	A	G	10: 102,320,918 (GRCm39)	L57S	probably damaging	Het
Nudt9	G	A	5: 104,212,971 (GRCm39)	R348H	probably benign	Het
Or10d5j	T	C	9: 39,867,979 (GRCm39)	Y84C	probably benign	Het
Or13a1	C	T	6: 116,470,725 (GRCm39)	P52S	probably benign	Het
Or3a1b	C	A	11: 74,012,650 (GRCm39)	D178E	probably benign	Het
Or4k49	T	C	2: 111,495,234 (GRCm39)	I221T	probably damaging	Het
Or5ac16	T	A	16: 59,022,271 (GRCm39)	I173F	possibly damaging	Het
Pafah1b1	T	C	11: 74,590,177 (GRCm39)		probably benign	Het
Piezo2	C	A	18: 63,211,911 (GRCm39)	M1291I	probably damaging	Het
Pik3ca	T	C	3: 32,498,016 (GRCm39)	F486S	probably benign	Het
Podnl1	C	T	8: 84,853,926 (GRCm39)	H99Y	probably benign	Het
Prdm6	A	T	18: 53,701,233 (GRCm39)	Y341F	probably damaging	Het
Prr5l	C	T	2: 101,588,854 (GRCm39)		probably null	Het
Psmd4	G	T	3: 94,944,012 (GRCm39)	T24N	possibly damaging	Het
Rbbp8nl	G	A	2: 179,922,667 (GRCm39)	T242M	probably benign	Het
Rsf1	GCG	GCGACG	7: 97,229,114 (GRCm39)		probably benign	Het
Rsf1	GGCGGCGGCGGCGGCGGCGGCGGCGGCGGC	GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC	7: 97,229,113 (GRCm39)		probably benign	Het
Scfd1	T	C	12: 51,469,769 (GRCm39)	V438A	probably benign	Het
Scn9a	A	G	2: 66,314,655 (GRCm39)	C1677R	probably damaging	Het
Sgsm2	T	A	11: 74,782,854 (GRCm39)	H34L	probably damaging	Het
Shank1	T	A	7: 43,993,747 (GRCm39)		probably null	Het
Smarca2	G	T	19: 26,650,124 (GRCm39)	E24*	probably null	Het
Sncaip	C	T	18: 53,004,434 (GRCm39)	H354Y	probably damaging	Het
St8sia2	T	A	7: 73,593,057 (GRCm39)	D333V	probably damaging	Het
Stxbp2	C	A	8: 3,692,672 (GRCm39)	R575S	probably benign	Het
Syt9	T	A	7: 107,024,314 (GRCm39)	V69D	probably damaging	Het
Tanc2	A	G	11: 105,689,558 (GRCm39)	N240S	probably benign	Het
Tcl1b1	T	C	12: 105,130,727 (GRCm39)	L70S	probably benign	Het
Tmem44	C	T	16: 30,362,219 (GRCm39)		probably null	Het
Tor1b	A	G	2: 30,846,931 (GRCm39)	R293G	probably benign	Het
Trim29	T	C	9: 43,222,615 (GRCm39)	V148A	probably benign	Het
Trmt10b	C	A	4: 45,314,378 (GRCm39)	Y271*	probably null	Het
Ubqln5	A	T	7: 103,778,095 (GRCm39)	V243E	possibly damaging	Het
Ubqln5	T	C	7: 103,778,134 (GRCm39)	D230G	probably damaging	Het
Ugt2b37	A	G	5: 87,402,193 (GRCm39)	F146S	probably damaging	Het
Vmn1r160	T	A	7: 22,570,827 (GRCm39)	V60E	probably damaging	Het
Vmn2r109	T	A	17: 20,774,185 (GRCm39)	D390V	probably damaging	Het
Vmn2r72	A	T	7: 85,398,369 (GRCm39)	V537D	probably benign	Het
Vmn2r84	A	G	10: 130,226,591 (GRCm39)	S416P	probably damaging	Het
Vmn2r98	C	A	17: 19,285,595 (GRCm39)	Y138*	probably null	Het
Vmn2r-ps158	T	C	7: 42,696,824 (GRCm39)	V620A	probably benign	Het
Xrra1	A	C	7: 99,560,227 (GRCm39)	E401A	probably damaging	Het
Zfp280d	T	A	9: 72,242,362 (GRCm39)	C688*	probably null	Het
Zfp3	T	A	11: 70,662,954 (GRCm39)	Y304*	probably null	Het
Zfp407	A	T	18: 84,577,461 (GRCm39)	D1217E	probably benign	Het
Zfp658	T	C	7: 43,223,245 (GRCm39)	Y507H	possibly damaging	Het
Zfyve16	T	C	13: 92,659,252 (GRCm39)	T220A	possibly damaging	Het
Zmym4	C	G	4: 126,796,463 (GRCm39)	K820N	possibly damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124,169,370 (GRCm39)	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124,200,791 (GRCm39)	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124,177,484 (GRCm39)	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124,174,308 (GRCm39)	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124,201,434 (GRCm39)	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124,169,276 (GRCm39)	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124,207,139 (GRCm39)	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0384:Vmn2r27	UTSW	6	124,200,871 (GRCm39)	missense	probably benign	0.01
R0582:Vmn2r27	UTSW	6	124,201,249 (GRCm39)	missense	probably benign	0.02
R0733:Vmn2r27	UTSW	6	124,169,147 (GRCm39)	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124,200,661 (GRCm39)	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124,177,583 (GRCm39)	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124,177,491 (GRCm39)	missense	probably benign
R1401:Vmn2r27	UTSW	6	124,168,591 (GRCm39)	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124,177,474 (GRCm39)	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124,177,649 (GRCm39)	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124,168,730 (GRCm39)	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1595:Vmn2r27	UTSW	6	124,208,574 (GRCm39)	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124,200,893 (GRCm39)	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124,177,636 (GRCm39)	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124,207,330 (GRCm39)	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1824:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1870:Vmn2r27	UTSW	6	124,201,170 (GRCm39)	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124,200,722 (GRCm39)	missense	probably damaging	1.00
R2069:Vmn2r27	UTSW	6	124,201,442 (GRCm39)	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124,177,510 (GRCm39)	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124,201,342 (GRCm39)	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124,207,351 (GRCm39)	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124,201,115 (GRCm39)	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124,207,135 (GRCm39)	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124,208,596 (GRCm39)	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124,201,141 (GRCm39)	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124,169,013 (GRCm39)	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124,169,103 (GRCm39)	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124,177,647 (GRCm39)	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124,208,686 (GRCm39)	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124,208,731 (GRCm39)	missense	probably benign
R6086:Vmn2r27	UTSW	6	124,168,958 (GRCm39)	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124,201,125 (GRCm39)	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124,169,369 (GRCm39)	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124,177,552 (GRCm39)	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124,201,312 (GRCm39)	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124,200,904 (GRCm39)	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124,168,711 (GRCm39)	missense	probably benign
R7176:Vmn2r27	UTSW	6	124,168,995 (GRCm39)	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124,174,276 (GRCm39)	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124,201,220 (GRCm39)	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124,168,980 (GRCm39)	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124,201,201 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124,169,040 (GRCm39)	missense	probably benign
R8266:Vmn2r27	UTSW	6	124,168,937 (GRCm39)	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124,169,404 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124,168,776 (GRCm39)	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124,169,168 (GRCm39)	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124,201,200 (GRCm39)	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124,207,188 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124,201,018 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124,174,224 (GRCm39)	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124,201,244 (GRCm39)	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124,168,856 (GRCm39)	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124,168,910 (GRCm39)	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124,168,637 (GRCm39)	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124,168,860 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTAATGCTACCTTCCATGGG -3'
(R):5'- GGCAATGTCTTGATCACAAATTCTG -3'

Sequencing Primer
(F):5'- CTAATGCTACCTTCCATGGGAGAGG -3'
(R):5'- TCAGATCACATGGATGAAATTCTGGG -3'

Posted On

2014-08-01