Mutagenetix > Incidental Mutation

Incidental Mutation 'R1950:Myh8'

217221

Institutional Source

Beutler Lab

Gene Symbol

Myh8

Ensembl Gene

ENSMUSG00000055775

Gene Name

myosin, heavy polypeptide 8, skeletal muscle, perinatal

Synonyms

Myhsp, MyHC-pn, Myhs-p, 4832426G23Rik

Accession Numbers

Essential gene?

Probably essential (E-score: 0.825) question?

Stock #

R1950 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67167950-67199460 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 67169830 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 50 (V50M)

Ref Sequence

ENSEMBL: ENSMUSP00000104325 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019625] [ENSMUST00000108685]

AlphaFold

P13542

Predicted Effect

probably benign
Transcript: ENSMUST00000019625
AA Change: V50M

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000019625
Gene: ENSMUSG00000055775
AA Change: V50M

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	37	76	2.1e-13	PFAM
MYSc	82	782	N/A	SMART
IQ	783	805	5.44e-3	SMART
Pfam:Myosin_tail_1	846	1927	2.4e-164	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108685
AA Change: V50M

PolyPhen 2 Score 0.752 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000104325
Gene: ENSMUSG00000055775
AA Change: V50M

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	37	78	3.8e-17	PFAM
Pfam:Myosin_head	90	172	1.7e-31	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000108686

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139052

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.8%
20x: 94.0%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a myosin heavy chain. The encoded protein forms a hexamer with two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. This gene is located in a cluster of related genes on chromosome 11. [provided by RefSeq, Jun 2013]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap9	A	G	5: 4,010,677 (GRCm39)	N478S	probably damaging	Het
Als2	T	A	1: 59,224,760 (GRCm39)		probably null	Het
Ankfy1	A	G	11: 72,651,155 (GRCm39)	Y1035C	probably damaging	Het
Ankrd11	T	C	8: 123,616,608 (GRCm39)	T2394A	probably damaging	Het
Axin1	G	C	17: 26,412,938 (GRCm39)	G780R	possibly damaging	Het
Carm1	A	T	9: 21,485,812 (GRCm39)	T127S	probably benign	Het
Ccdc73	T	C	2: 104,757,280 (GRCm39)	I81T	probably benign	Het
Cdh12	T	C	15: 21,237,965 (GRCm39)	Y67H	probably damaging	Het
Cfap65	C	A	1: 74,946,819 (GRCm39)	G1297V	probably damaging	Het
Cfap74	T	C	4: 155,511,887 (GRCm39)		probably null	Het
Chit1	A	G	1: 134,078,968 (GRCm39)	Y426C	probably damaging	Het
Clpp	A	G	17: 57,303,039 (GRCm39)		probably benign	Het
Clrn3	T	G	7: 135,115,813 (GRCm39)	Y179S	possibly damaging	Het
Cntn5	A	C	9: 9,781,774 (GRCm39)	M635R	probably damaging	Het
Col12a1	A	T	9: 79,537,831 (GRCm39)	S2546T	possibly damaging	Het
Ctla2b	T	C	13: 61,043,863 (GRCm39)	N102S	possibly damaging	Het
Cwf19l2	A	T	9: 3,418,674 (GRCm39)	I154F	probably benign	Het
Dffa	C	A	4: 149,188,839 (GRCm39)	S44R	probably benign	Het
Ehbp1	T	A	11: 22,009,228 (GRCm39)	K930M	probably damaging	Het
Erc2	T	A	14: 27,634,857 (GRCm39)	S473T	probably damaging	Het
Fbxw24	A	T	9: 109,434,481 (GRCm39)	L373H	probably benign	Het
Fer1l4	T	C	2: 155,890,194 (GRCm39)	I244V	probably damaging	Het
Fgfr2	G	A	7: 129,800,211 (GRCm39)	T245M	probably damaging	Het
Garin1b	C	T	6: 29,335,815 (GRCm39)		probably null	Het
Gbp9	T	A	5: 105,229,112 (GRCm39)	M512L	probably benign	Het
Glg1	T	A	8: 111,892,271 (GRCm39)	K251I	possibly damaging	Het
Gm10553	A	G	1: 85,078,141 (GRCm39)	D86G	possibly damaging	Het
Gm5622	T	C	14: 51,893,229 (GRCm39)	V52A	probably benign	Het
Gucy1b1	A	G	3: 81,952,716 (GRCm39)	V239A	probably benign	Het
Gzma	A	G	13: 113,230,463 (GRCm39)	L246P	probably damaging	Het
Hibadh	C	A	6: 52,533,448 (GRCm39)	A223S	probably benign	Het
Hydin	C	A	8: 111,336,619 (GRCm39)	T5132N	possibly damaging	Het
Insrr	A	G	3: 87,721,820 (GRCm39)	N1198S	probably damaging	Het
Ivl	T	C	3: 92,479,420 (GRCm39)	E215G	possibly damaging	Het
Jmjd1c	A	G	10: 67,075,701 (GRCm39)	D2037G	possibly damaging	Het
Kcna3	G	A	3: 106,944,988 (GRCm39)	C417Y	probably damaging	Het
Klra2	T	C	6: 131,207,078 (GRCm39)	N177S	probably benign	Het
Llgl2	T	C	11: 115,741,892 (GRCm39)	S645P	probably damaging	Het
Lrrc15	T	C	16: 30,092,649 (GRCm39)	E230G	probably benign	Het
Magel2	C	A	7: 62,028,163 (GRCm39)	Q356K	possibly damaging	Het
Mcm6	A	G	1: 128,273,726 (GRCm39)	V368A	probably benign	Het
Myrf	A	T	19: 10,195,554 (GRCm39)	F419I	possibly damaging	Het
Nrbp1	T	A	5: 31,403,157 (GRCm39)	I210N	probably damaging	Het
Or10ak16	G	A	4: 118,750,537 (GRCm39)	V86M	probably benign	Het
Or52i2	A	G	7: 102,319,684 (GRCm39)	M186V	probably benign	Het
Otud4	G	A	8: 80,372,961 (GRCm39)	R93H	probably damaging	Het
Pank4	T	A	4: 155,056,977 (GRCm39)	M390K	probably benign	Het
Pecam1	A	G	11: 106,576,029 (GRCm39)	V401A	probably damaging	Het
Prdm6	C	A	18: 53,669,796 (GRCm39)	T138K	possibly damaging	Het
Prl2c2	G	C	13: 13,176,786 (GRCm39)	T47R	probably damaging	Het
Prpf8	A	G	11: 75,387,337 (GRCm39)	E1206G	possibly damaging	Het
Prr30	A	T	14: 101,435,377 (GRCm39)	I395N	probably benign	Het
Rab27a	G	A	9: 72,982,751 (GRCm39)	G19R	probably damaging	Het
Ramacl	A	G	13: 67,055,269 (GRCm39)		probably benign	Het
Rrp12	A	G	19: 41,881,029 (GRCm39)	V134A	probably damaging	Het
Scly	A	G	1: 91,233,116 (GRCm39)	T76A	probably benign	Het
Scube3	T	C	17: 28,383,274 (GRCm39)	S439P	possibly damaging	Het
Shf	G	A	2: 122,199,163 (GRCm39)	P51S	probably damaging	Het
Sipa1l1	T	C	12: 82,388,233 (GRCm39)	F153S	probably damaging	Het
Slc25a30	T	G	14: 76,007,007 (GRCm39)	K163T	possibly damaging	Het
Slc26a8	T	A	17: 28,863,614 (GRCm39)	D715V	probably benign	Het
Smr2	AT	ATT	5: 88,256,683 (GRCm39)		probably null	Het
Smr2	C	CT	5: 88,256,685 (GRCm39)		probably null	Het
Sp6	T	G	11: 96,912,940 (GRCm39)	S218A	probably benign	Het
Spata21	T	C	4: 140,838,716 (GRCm39)	V589A	probably damaging	Het
Sycp2	T	C	2: 178,044,593 (GRCm39)	I71V	probably benign	Het
Syne2	G	T	12: 75,999,644 (GRCm39)	G2347C	probably benign	Het
Tenm2	C	T	11: 35,954,004 (GRCm39)	G1236R	possibly damaging	Het
Thbs4	A	T	13: 92,906,079 (GRCm39)	N387K	probably damaging	Het
Tmem184a	T	C	5: 139,793,381 (GRCm39)	D216G	probably damaging	Het
Tprg1	T	A	16: 25,136,098 (GRCm39)	S30T	possibly damaging	Het
Trip12	A	T	1: 84,738,522 (GRCm39)	W778R	probably damaging	Het
Ttpa	T	C	4: 20,008,633 (GRCm39)	L65P	probably damaging	Het
Ush2a	A	T	1: 188,487,382 (GRCm39)	N3050I	probably damaging	Het
Xpot	T	C	10: 121,455,053 (GRCm39)	I30V	probably benign	Het
Zfp944	A	G	17: 22,558,681 (GRCm39)	S189P	probably benign	Het
Zftraf1	A	G	15: 76,543,417 (GRCm39)		probably null	Het

Other mutations in Myh8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Myh8	APN	11	67,174,644 (GRCm39)	missense	probably damaging	0.97
IGL01020:Myh8	APN	11	67,174,229 (GRCm39)	missense	probably damaging	0.99
IGL01348:Myh8	APN	11	67,188,606 (GRCm39)	missense	probably damaging	1.00
IGL01382:Myh8	APN	11	67,192,799 (GRCm39)	missense	probably damaging	1.00
IGL01454:Myh8	APN	11	67,174,422 (GRCm39)	missense	probably damaging	1.00
IGL01457:Myh8	APN	11	67,183,505 (GRCm39)	missense	probably benign	0.00
IGL01472:Myh8	APN	11	67,179,205 (GRCm39)	splice site	probably benign
IGL01473:Myh8	APN	11	67,192,651 (GRCm39)	critical splice donor site	probably null
IGL01613:Myh8	APN	11	67,192,536 (GRCm39)	missense	probably benign	0.11
IGL01763:Myh8	APN	11	67,177,245 (GRCm39)	missense	probably benign	0.01
IGL01828:Myh8	APN	11	67,194,652 (GRCm39)	missense	possibly damaging	0.82
IGL01862:Myh8	APN	11	67,180,520 (GRCm39)	nonsense	probably null
IGL01905:Myh8	APN	11	67,175,477 (GRCm39)	missense	possibly damaging	0.90
IGL02280:Myh8	APN	11	67,174,198 (GRCm39)	unclassified	probably benign
IGL02386:Myh8	APN	11	67,185,266 (GRCm39)	missense	probably damaging	0.99
IGL02449:Myh8	APN	11	67,185,440 (GRCm39)	critical splice donor site	probably null
IGL02500:Myh8	APN	11	67,196,536 (GRCm39)	missense	probably benign	0.00
IGL02745:Myh8	APN	11	67,188,327 (GRCm39)	missense	possibly damaging	0.88
IGL02799:Myh8	APN	11	67,192,418 (GRCm39)	splice site	probably benign
IGL03063:Myh8	APN	11	67,179,031 (GRCm39)	missense	probably benign	0.00
IGL03223:Myh8	APN	11	67,174,644 (GRCm39)	missense	probably damaging	0.97
IGL03336:Myh8	APN	11	67,175,528 (GRCm39)	missense	probably damaging	1.00
IGL03338:Myh8	APN	11	67,189,172 (GRCm39)	missense	probably damaging	1.00
IGL03351:Myh8	APN	11	67,194,739 (GRCm39)	missense	possibly damaging	0.94
IGL03392:Myh8	APN	11	67,185,244 (GRCm39)	missense	probably damaging	1.00
BB003:Myh8	UTSW	11	67,169,732 (GRCm39)	missense	possibly damaging	0.94
BB009:Myh8	UTSW	11	67,185,430 (GRCm39)	missense	probably benign	0.00
BB013:Myh8	UTSW	11	67,169,732 (GRCm39)	missense	possibly damaging	0.94
BB019:Myh8	UTSW	11	67,185,430 (GRCm39)	missense	probably benign	0.00
PIT4354001:Myh8	UTSW	11	67,180,456 (GRCm39)	missense	probably benign	0.01
R0012:Myh8	UTSW	11	67,190,847 (GRCm39)	missense	probably benign	0.02
R0016:Myh8	UTSW	11	67,189,351 (GRCm39)	missense	probably damaging	1.00
R0016:Myh8	UTSW	11	67,189,351 (GRCm39)	missense	probably damaging	1.00
R0115:Myh8	UTSW	11	67,197,090 (GRCm39)	splice site	probably benign
R0131:Myh8	UTSW	11	67,183,014 (GRCm39)	missense	probably damaging	0.96
R0131:Myh8	UTSW	11	67,183,014 (GRCm39)	missense	probably damaging	0.96
R0132:Myh8	UTSW	11	67,183,014 (GRCm39)	missense	probably damaging	0.96
R0238:Myh8	UTSW	11	67,192,518 (GRCm39)	missense	probably benign	0.00
R0238:Myh8	UTSW	11	67,192,518 (GRCm39)	missense	probably benign	0.00
R0239:Myh8	UTSW	11	67,192,518 (GRCm39)	missense	probably benign	0.00
R0239:Myh8	UTSW	11	67,192,518 (GRCm39)	missense	probably benign	0.00
R0393:Myh8	UTSW	11	67,196,843 (GRCm39)	splice site	probably benign
R0453:Myh8	UTSW	11	67,183,731 (GRCm39)	missense	probably benign	0.03
R0454:Myh8	UTSW	11	67,194,591 (GRCm39)	nonsense	probably null
R0466:Myh8	UTSW	11	67,189,405 (GRCm39)	missense	probably benign	0.01
R0487:Myh8	UTSW	11	67,192,837 (GRCm39)	missense	probably benign
R0511:Myh8	UTSW	11	67,175,333 (GRCm39)	missense	probably benign	0.01
R0557:Myh8	UTSW	11	67,192,624 (GRCm39)	missense	possibly damaging	0.88
R0589:Myh8	UTSW	11	67,189,453 (GRCm39)	missense	probably benign	0.00
R0658:Myh8	UTSW	11	67,175,358 (GRCm39)	critical splice donor site	probably null
R0782:Myh8	UTSW	11	67,180,580 (GRCm39)	missense	probably benign	0.16
R0829:Myh8	UTSW	11	67,174,326 (GRCm39)	unclassified	probably benign
R0845:Myh8	UTSW	11	67,177,090 (GRCm39)	missense	probably damaging	1.00
R0930:Myh8	UTSW	11	67,196,824 (GRCm39)	missense	possibly damaging	0.93
R0972:Myh8	UTSW	11	67,188,585 (GRCm39)	missense	probably damaging	1.00
R1132:Myh8	UTSW	11	67,187,957 (GRCm39)	nonsense	probably null
R1417:Myh8	UTSW	11	67,197,011 (GRCm39)	missense	probably damaging	1.00
R1478:Myh8	UTSW	11	67,183,551 (GRCm39)	missense	probably benign	0.23
R1497:Myh8	UTSW	11	67,180,638 (GRCm39)	missense	probably benign	0.00
R1605:Myh8	UTSW	11	67,192,497 (GRCm39)	missense	probably damaging	0.99
R1701:Myh8	UTSW	11	67,170,964 (GRCm39)	missense	probably damaging	1.00
R1989:Myh8	UTSW	11	67,183,550 (GRCm39)	missense	probably benign	0.00
R2010:Myh8	UTSW	11	67,187,990 (GRCm39)	nonsense	probably null
R2095:Myh8	UTSW	11	67,177,050 (GRCm39)	missense	probably benign	0.00
R2132:Myh8	UTSW	11	67,183,702 (GRCm39)	missense	probably damaging	1.00
R2152:Myh8	UTSW	11	67,185,295 (GRCm39)	missense	probably damaging	0.97
R2229:Myh8	UTSW	11	67,199,174 (GRCm39)	missense	probably damaging	0.98
R2302:Myh8	UTSW	11	67,177,065 (GRCm39)	missense	probably damaging	1.00
R2364:Myh8	UTSW	11	67,185,344 (GRCm39)	missense	probably benign	0.03
R2429:Myh8	UTSW	11	67,194,723 (GRCm39)	missense	probably benign	0.21
R2880:Myh8	UTSW	11	67,188,090 (GRCm39)	missense	probably damaging	0.97
R3692:Myh8	UTSW	11	67,192,744 (GRCm39)	missense	probably damaging	0.98
R3756:Myh8	UTSW	11	67,175,443 (GRCm39)	unclassified	probably benign
R3924:Myh8	UTSW	11	67,187,963 (GRCm39)	missense	probably damaging	0.99
R4172:Myh8	UTSW	11	67,183,247 (GRCm39)	missense	probably damaging	1.00
R4255:Myh8	UTSW	11	67,190,560 (GRCm39)	missense	probably benign
R4621:Myh8	UTSW	11	67,177,084 (GRCm39)	missense	probably damaging	1.00
R4623:Myh8	UTSW	11	67,177,084 (GRCm39)	missense	probably damaging	1.00
R4790:Myh8	UTSW	11	67,170,789 (GRCm39)	missense	probably damaging	0.99
R4914:Myh8	UTSW	11	67,183,510 (GRCm39)	missense	probably damaging	1.00
R5074:Myh8	UTSW	11	67,196,742 (GRCm39)	missense	possibly damaging	0.79
R5119:Myh8	UTSW	11	67,189,184 (GRCm39)	missense	probably damaging	1.00
R5159:Myh8	UTSW	11	67,179,179 (GRCm39)	missense	probably damaging	0.99
R5229:Myh8	UTSW	11	67,175,310 (GRCm39)	missense	probably damaging	0.96
R5320:Myh8	UTSW	11	67,177,089 (GRCm39)	missense	probably damaging	1.00
R5455:Myh8	UTSW	11	67,192,244 (GRCm39)	missense	possibly damaging	0.59
R5523:Myh8	UTSW	11	67,196,788 (GRCm39)	missense	possibly damaging	0.95
R5540:Myh8	UTSW	11	67,177,266 (GRCm39)	missense	probably benign	0.00
R5726:Myh8	UTSW	11	67,185,392 (GRCm39)	missense	possibly damaging	0.79
R5770:Myh8	UTSW	11	67,188,026 (GRCm39)	missense	probably damaging	1.00
R6135:Myh8	UTSW	11	67,188,326 (GRCm39)	missense	possibly damaging	0.51
R6253:Myh8	UTSW	11	67,192,793 (GRCm39)	missense	probably benign	0.06
R6318:Myh8	UTSW	11	67,190,167 (GRCm39)	missense	probably benign	0.00
R6432:Myh8	UTSW	11	67,189,405 (GRCm39)	missense	probably benign	0.01
R6452:Myh8	UTSW	11	67,196,565 (GRCm39)	missense	possibly damaging	0.88
R6452:Myh8	UTSW	11	67,183,275 (GRCm39)	missense	probably benign	0.27
R6512:Myh8	UTSW	11	67,180,488 (GRCm39)	nonsense	probably null
R6714:Myh8	UTSW	11	67,197,775 (GRCm39)	missense	probably damaging	1.00
R6842:Myh8	UTSW	11	67,175,481 (GRCm39)	missense	probably damaging	1.00
R7007:Myh8	UTSW	11	67,179,142 (GRCm39)	missense	probably benign	0.03
R7025:Myh8	UTSW	11	67,188,365 (GRCm39)	missense	probably benign	0.02
R7086:Myh8	UTSW	11	67,183,453 (GRCm39)	splice site	probably null
R7098:Myh8	UTSW	11	67,169,879 (GRCm39)	missense	probably benign	0.03
R7498:Myh8	UTSW	11	67,174,263 (GRCm39)	missense	possibly damaging	0.80
R7716:Myh8	UTSW	11	67,189,478 (GRCm39)	missense	possibly damaging	0.51
R7765:Myh8	UTSW	11	67,194,481 (GRCm39)	missense	probably benign	0.44
R7825:Myh8	UTSW	11	67,194,538 (GRCm39)	missense	possibly damaging	0.94
R7921:Myh8	UTSW	11	67,174,644 (GRCm39)	missense	probably damaging	0.97
R7926:Myh8	UTSW	11	67,169,732 (GRCm39)	missense	possibly damaging	0.94
R7932:Myh8	UTSW	11	67,185,430 (GRCm39)	missense	probably benign	0.00
R8003:Myh8	UTSW	11	67,190,586 (GRCm39)	missense	probably damaging	1.00
R8028:Myh8	UTSW	11	67,194,502 (GRCm39)	missense	possibly damaging	0.65
R8121:Myh8	UTSW	11	67,180,647 (GRCm39)	missense	probably benign	0.00
R8125:Myh8	UTSW	11	67,190,598 (GRCm39)	missense	possibly damaging	0.94
R8170:Myh8	UTSW	11	67,179,092 (GRCm39)	missense	probably benign	0.30
R8277:Myh8	UTSW	11	67,183,735 (GRCm39)	missense	probably benign	0.10
R8304:Myh8	UTSW	11	67,195,162 (GRCm39)	missense	possibly damaging	0.72
R8431:Myh8	UTSW	11	67,174,440 (GRCm39)	missense	possibly damaging	0.94
R8535:Myh8	UTSW	11	67,169,741 (GRCm39)	missense	probably damaging	1.00
R8795:Myh8	UTSW	11	67,174,203 (GRCm39)	critical splice acceptor site	probably benign
R8858:Myh8	UTSW	11	67,192,820 (GRCm39)	missense	possibly damaging	0.67
R8927:Myh8	UTSW	11	67,174,081 (GRCm39)	missense	probably benign	0.10
R8928:Myh8	UTSW	11	67,174,081 (GRCm39)	missense	probably benign	0.10
R9031:Myh8	UTSW	11	67,190,141 (GRCm39)	missense	possibly damaging	0.49
R9172:Myh8	UTSW	11	67,183,260 (GRCm39)	missense	possibly damaging	0.82
R9252:Myh8	UTSW	11	67,177,302 (GRCm39)	missense	probably damaging	1.00
R9365:Myh8	UTSW	11	67,174,632 (GRCm39)	missense	probably benign	0.42
R9468:Myh8	UTSW	11	67,197,730 (GRCm39)	missense	probably damaging	1.00
R9564:Myh8	UTSW	11	67,177,215 (GRCm39)	missense	probably benign	0.40
R9565:Myh8	UTSW	11	67,177,215 (GRCm39)	missense	probably benign	0.40
T0722:Myh8	UTSW	11	67,195,262 (GRCm39)	missense	probably benign	0.41
Z1088:Myh8	UTSW	11	67,189,418 (GRCm39)	missense	probably damaging	1.00
Z1176:Myh8	UTSW	11	67,194,500 (GRCm39)	missense	probably damaging	1.00
Z1177:Myh8	UTSW	11	67,199,181 (GRCm39)	missense	possibly damaging	0.64
Z1177:Myh8	UTSW	11	67,192,250 (GRCm39)	missense	probably damaging	0.99
Z1187:Myh8	UTSW	11	67,188,312 (GRCm39)	missense	probably benign
Z1188:Myh8	UTSW	11	67,188,312 (GRCm39)	missense	probably benign
Z1190:Myh8	UTSW	11	67,188,312 (GRCm39)	missense	probably benign
Z1191:Myh8	UTSW	11	67,188,312 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTCCTCAAAAGCTGAAACCTC -3'
(R):5'- TGTAGCCTGCACCCCTTTAG -3'

Sequencing Primer
(F):5'- CTCGGCTAAATAAGTAGCAGCCATG -3'
(R):5'- ATCTTGGGACCAAATCCTAGGAG -3'

Posted On

2014-08-01