Mutagenetix > Incidental Mutation

Incidental Mutation 'R1955:Kmt2b'

217673

Institutional Source

Beutler Lab

Gene Symbol

Kmt2b

Ensembl Gene

ENSMUSG00000006307

Gene Name

lysine (K)-specific methyltransferase 2B

Synonyms

2610014H22Rik, Mll2, Wbp7

MMRRC Submission

039969-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1955 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

30268283-30288151 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 30274776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1976 (M1976K)

Ref Sequence

ENSEMBL: ENSMUSP00000103789 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006470] [ENSMUST00000108154]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000006470
AA Change: M1985K

PolyPhen 2 Score 0.896 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000006470
Gene: ENSMUSG00000006307
AA Change: M1985K

Domain	Start	End	E-Value	Type
AT_hook	18	30	2.82e2	SMART
low complexity region	66	106	N/A	INTRINSIC
low complexity region	110	122	N/A	INTRINSIC
AT_hook	149	159	2.4e2	SMART
AT_hook	218	230	1.95e2	SMART
low complexity region	249	263	N/A	INTRINSIC
low complexity region	272	302	N/A	INTRINSIC
coiled coil region	353	413	N/A	INTRINSIC
AT_hook	476	488	5.47e-1	SMART
low complexity region	501	517	N/A	INTRINSIC
low complexity region	578	606	N/A	INTRINSIC
low complexity region	621	657	N/A	INTRINSIC
low complexity region	673	700	N/A	INTRINSIC
low complexity region	715	728	N/A	INTRINSIC
low complexity region	738	777	N/A	INTRINSIC
low complexity region	910	922	N/A	INTRINSIC
Pfam:zf-CXXC	963	1010	7.2e-15	PFAM
low complexity region	1039	1061	N/A	INTRINSIC
low complexity region	1103	1115	N/A	INTRINSIC
PHD	1209	1256	1.25e-5	SMART
PHD	1257	1307	5.4e-10	SMART
PHD	1343	1400	1.27e-6	SMART
low complexity region	1415	1427	N/A	INTRINSIC
PHD	1646	1692	3.82e-1	SMART
FYRN	1745	1788	3.25e-19	SMART
low complexity region	1881	1899	N/A	INTRINSIC
low complexity region	1912	1942	N/A	INTRINSIC
low complexity region	1961	1978	N/A	INTRINSIC
low complexity region	1991	2003	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2048	2061	N/A	INTRINSIC
low complexity region	2087	2105	N/A	INTRINSIC
low complexity region	2127	2138	N/A	INTRINSIC
low complexity region	2215	2235	N/A	INTRINSIC
low complexity region	2239	2270	N/A	INTRINSIC
low complexity region	2396	2406	N/A	INTRINSIC
FYRC	2419	2504	4.83e-36	SMART
SET	2581	2703	1.67e-42	SMART
PostSET	2705	2721	4.65e-4	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108154
AA Change: M1976K

PolyPhen 2 Score 0.896 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000103789
Gene: ENSMUSG00000006307
AA Change: M1976K

Domain	Start	End	E-Value	Type
AT_hook	18	30	2.82e2	SMART
low complexity region	66	106	N/A	INTRINSIC
low complexity region	110	122	N/A	INTRINSIC
AT_hook	149	159	2.4e2	SMART
AT_hook	218	230	1.95e2	SMART
low complexity region	249	263	N/A	INTRINSIC
low complexity region	272	302	N/A	INTRINSIC
coiled coil region	353	413	N/A	INTRINSIC
AT_hook	476	488	5.47e-1	SMART
low complexity region	501	517	N/A	INTRINSIC
low complexity region	578	606	N/A	INTRINSIC
low complexity region	621	657	N/A	INTRINSIC
low complexity region	673	700	N/A	INTRINSIC
low complexity region	715	728	N/A	INTRINSIC
low complexity region	738	777	N/A	INTRINSIC
low complexity region	910	922	N/A	INTRINSIC
Pfam:zf-CXXC	963	1010	1e-14	PFAM
low complexity region	1039	1061	N/A	INTRINSIC
low complexity region	1103	1115	N/A	INTRINSIC
PHD	1209	1256	1.25e-5	SMART
PHD	1257	1307	5.4e-10	SMART
PHD	1343	1400	1.27e-6	SMART
low complexity region	1415	1427	N/A	INTRINSIC
PHD	1646	1692	3.82e-1	SMART
FYRN	1745	1788	3.25e-19	SMART
low complexity region	1872	1890	N/A	INTRINSIC
low complexity region	1903	1933	N/A	INTRINSIC
low complexity region	1952	1969	N/A	INTRINSIC
low complexity region	1982	1994	N/A	INTRINSIC
low complexity region	2004	2017	N/A	INTRINSIC
low complexity region	2039	2052	N/A	INTRINSIC
low complexity region	2078	2096	N/A	INTRINSIC
low complexity region	2118	2129	N/A	INTRINSIC
low complexity region	2206	2226	N/A	INTRINSIC
low complexity region	2230	2261	N/A	INTRINSIC
low complexity region	2383	2398	N/A	INTRINSIC
FYRC	2411	2496	4.83e-36	SMART
SET	2573	2695	1.67e-42	SMART
PostSET	2697	2713	4.65e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125836

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128456

Predicted Effect

unknown
Transcript: ENSMUST00000131002
AA Change: M1276K

SMART Domains

Protein: ENSMUSP00000118486
Gene: ENSMUSG00000006307
AA Change: M1276K

Domain	Start	End	E-Value	Type
low complexity region	7	20	N/A	INTRINSIC
low complexity region	30	69	N/A	INTRINSIC
low complexity region	202	214	N/A	INTRINSIC
Pfam:zf-CXXC	255	302	5.2e-15	PFAM
low complexity region	331	353	N/A	INTRINSIC
low complexity region	395	407	N/A	INTRINSIC
PHD	501	548	1.25e-5	SMART
PHD	549	599	5.4e-10	SMART
PHD	635	692	1.27e-6	SMART
low complexity region	707	719	N/A	INTRINSIC
PHD	938	984	3.82e-1	SMART
FYRN	1037	1080	3.25e-19	SMART
low complexity region	1173	1191	N/A	INTRINSIC
low complexity region	1204	1234	N/A	INTRINSIC
low complexity region	1253	1270	N/A	INTRINSIC
low complexity region	1283	1295	N/A	INTRINSIC
low complexity region	1305	1318	N/A	INTRINSIC
low complexity region	1340	1353	N/A	INTRINSIC
low complexity region	1379	1397	N/A	INTRINSIC
low complexity region	1419	1430	N/A	INTRINSIC
low complexity region	1507	1527	N/A	INTRINSIC
low complexity region	1531	1562	N/A	INTRINSIC
low complexity region	1684	1699	N/A	INTRINSIC
FYRC	1712	1797	4.83e-36	SMART
SET	1874	1996	1.67e-42	SMART
PostSET	1998	2014	4.65e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136959

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152931

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous disruption of this gene leads to embryonic growth retardation, abnormal somite development, neural tube defects, increased apoptosis, and complete embryonic lethality. Homozygotes for a hypomorphic allele show embryonic growth arrest, altered DNA methylation, and reduced female fertility. [provided by MGI curators]

Allele List at MGI

All alleles(8) : Targeted, knock-out(2) Targeted, other(2) Gene trapped(4)

Other mutations in this stock

Total: 101 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700019A02Rik	T	A	1: 53,202,400 (GRCm39)	K128N	probably benign	Het
Acadm	A	C	3: 153,635,188 (GRCm39)	F309V	probably damaging	Het
Adam6b	C	A	12: 113,455,436 (GRCm39)	A751E	probably benign	Het
Adcy9	A	G	16: 4,236,523 (GRCm39)	I296T	possibly damaging	Het
Arhgap45	A	G	10: 79,862,326 (GRCm39)	E471G	probably benign	Het
Atcay	T	C	10: 81,050,627 (GRCm39)	D96G	possibly damaging	Het
B3galt4	T	A	17: 34,169,606 (GRCm39)	R211*	probably null	Het
Bpi	T	C	2: 158,116,635 (GRCm39)	I344T	probably damaging	Het
Btnl4	T	C	17: 34,691,904 (GRCm39)	K233E	possibly damaging	Het
Cars2	A	T	8: 11,600,286 (GRCm39)	Y68N	probably damaging	Het
Cd207	T	A	6: 83,648,757 (GRCm39)	R302W	probably benign	Het
Cdc5l	T	C	17: 45,737,442 (GRCm39)		probably null	Het
Chaf1a	C	T	17: 56,354,540 (GRCm39)	T270I	unknown	Het
Col4a1	C	T	8: 11,258,228 (GRCm39)		probably null	Het
Col7a1	G	T	9: 108,784,732 (GRCm39)	V187L	unknown	Het
Cry1	G	A	10: 84,980,042 (GRCm39)	T422I	probably benign	Het
Csgalnact1	C	T	8: 68,825,319 (GRCm39)	V392I	probably benign	Het
Cyp2c38	A	G	19: 39,393,131 (GRCm39)	L312P	probably damaging	Het
Ddx20	G	A	3: 105,586,878 (GRCm39)	T489M	possibly damaging	Het
Decr1	A	T	4: 15,924,256 (GRCm39)	N221K	probably benign	Het
Dennd4a	A	T	9: 64,759,749 (GRCm39)	T285S	probably benign	Het
Dgka	A	G	10: 128,566,058 (GRCm39)		probably null	Het
Dmd	A	T	X: 82,922,163 (GRCm39)	M1478L	probably benign	Het
Dvl1	G	A	4: 155,942,486 (GRCm39)	R584Q	possibly damaging	Het
Dync1li1	A	T	9: 114,550,814 (GRCm39)	S450C	probably damaging	Het
Ehbp1l1	A	G	19: 5,760,697 (GRCm39)	V1558A	possibly damaging	Het
Esr1	T	A	10: 4,807,125 (GRCm39)	M347K	probably damaging	Het
F2	T	A	2: 91,463,440 (GRCm39)	H148L	probably benign	Het
Fezf2	T	C	14: 12,342,644 (GRCm38)	N407S	probably benign	Het
Fscn3	T	A	6: 28,430,235 (GRCm39)	M135K	possibly damaging	Het
Ganab	T	G	19: 8,888,980 (GRCm39)	Y560*	probably null	Het
Garem2	T	C	5: 30,313,268 (GRCm39)	V44A	probably benign	Het
Gja4	A	G	4: 127,206,242 (GRCm39)	W174R	probably damaging	Het
Gja5	A	T	3: 96,958,957 (GRCm39)	H338L	probably benign	Het
Gm43517	A	G	12: 49,436,672 (GRCm39)		probably benign	Het
Iqch	A	T	9: 63,455,298 (GRCm39)	D166E	probably benign	Het
Kifc5b	T	A	17: 27,145,271 (GRCm39)		probably null	Het
Leng1	A	T	7: 3,668,415 (GRCm39)	V11D	probably damaging	Het
Lrit3	A	G	3: 129,594,130 (GRCm39)	V149A	probably benign	Het
Lrpap1	A	G	5: 35,259,756 (GRCm39)	L114P	probably damaging	Het
Marveld3	T	A	8: 110,686,380 (GRCm39)	D162V	probably benign	Het
Mon2	A	T	10: 122,874,388 (GRCm39)	I320N	probably damaging	Het
Morc2b	T	C	17: 33,356,464 (GRCm39)	Y436C	probably damaging	Het
Myo7a	A	T	7: 97,704,128 (GRCm39)	V1928D	probably damaging	Het
Ncoa6	A	G	2: 155,248,741 (GRCm39)	V1521A	possibly damaging	Het
Nexmif	A	G	X: 103,127,559 (GRCm39)	S1453P	possibly damaging	Het
Nudt16l1	A	G	16: 4,758,189 (GRCm39)	M182V	probably benign	Het
Obp2b	A	T	2: 25,628,563 (GRCm39)	I106L	probably benign	Het
Or10ag59	T	A	2: 87,406,349 (GRCm39)	L307Q	probably damaging	Het
Or4a2	T	A	2: 89,248,755 (GRCm39)	M1L	probably damaging	Het
Or5h18	T	A	16: 58,847,774 (GRCm39)	R165S	probably damaging	Het
Or8b43	T	C	9: 38,360,984 (GRCm39)	I272T	probably benign	Het
Parl	T	A	16: 20,121,077 (GRCm39)	M1L	possibly damaging	Het
Pde7a	G	A	3: 19,281,963 (GRCm39)	A429V	probably damaging	Het
Pkd1l2	T	A	8: 117,770,100 (GRCm39)	M1119L	probably benign	Het
Plch1	C	T	3: 63,662,688 (GRCm39)	V272I	probably damaging	Het
Plekha4	G	A	7: 45,203,330 (GRCm39)	G740D	probably damaging	Het
Pnliprp1	A	G	19: 58,723,404 (GRCm39)	D265G	possibly damaging	Het
Pola1	A	C	X: 92,640,867 (GRCm39)	V384G	probably damaging	Het
Pon3	T	A	6: 5,230,774 (GRCm39)	D251V	probably benign	Het
Pot1b	A	G	17: 55,981,067 (GRCm39)	C316R	possibly damaging	Het
Prkaca	T	A	8: 84,714,946 (GRCm39)	V116E	probably damaging	Het
Prpf6	T	G	2: 181,273,870 (GRCm39)	M338R	probably benign	Het
Rasgef1c	A	G	11: 49,866,542 (GRCm39)	H382R	possibly damaging	Het
Rlig1	A	G	10: 100,413,166 (GRCm39)	V91A	probably damaging	Het
Rps25	C	T	9: 44,321,305 (GRCm39)	T113I	probably benign	Het
Serpinb1b	G	T	13: 33,269,422 (GRCm39)	A52S	probably benign	Het
Sh3yl1	A	G	12: 30,972,332 (GRCm39)	K34E	possibly damaging	Het
Siah2	C	T	3: 58,583,518 (GRCm39)	R256Q	probably damaging	Het
Slc24a2	A	G	4: 86,991,481 (GRCm39)	L404P	probably damaging	Het
Slc28a2	G	A	2: 122,278,347 (GRCm39)	C122Y	probably benign	Het
Slc4a8	T	C	15: 100,705,257 (GRCm39)	I821T	probably damaging	Het
Slc5a3	T	C	16: 91,874,762 (GRCm39)	V273A	possibly damaging	Het
Spag7	T	C	11: 70,555,844 (GRCm39)	Q61R	probably benign	Het
Syt7	A	T	19: 10,395,402 (GRCm39)	I71F	probably damaging	Het
Tars2	T	C	3: 95,654,766 (GRCm39)	N413S	probably damaging	Het
Tex14	T	A	11: 87,400,447 (GRCm39)	L413Q	probably damaging	Het
Tjp3	T	C	10: 81,113,833 (GRCm39)	E475G	probably damaging	Het
Tmem131l	A	G	3: 83,868,851 (GRCm39)	S175P	probably damaging	Het
Tmem144	C	T	3: 79,734,164 (GRCm39)	V180M	probably benign	Het
Tmem200c	A	T	17: 69,147,956 (GRCm39)	I180F	probably damaging	Het
Tmem232	G	T	17: 65,791,482 (GRCm39)	H129N	probably benign	Het
Tmprss11g	T	A	5: 86,646,391 (GRCm39)	I59F	probably damaging	Het
Tmprss2	T	A	16: 97,368,377 (GRCm39)		probably null	Het
Tor1aip2	T	A	1: 155,927,588 (GRCm39)		probably benign	Het
Trim61	T	C	8: 65,466,044 (GRCm39)	I406V	possibly damaging	Het
Trpv5	T	C	6: 41,634,871 (GRCm39)	D486G	probably damaging	Het
Try5	T	C	6: 41,288,703 (GRCm39)	E64G	probably benign	Het
Txlnb	A	G	10: 17,675,168 (GRCm39)	E107G	probably damaging	Het
Ube2frt	A	G	12: 36,140,595 (GRCm39)		probably benign	Het
Uckl1	T	C	2: 181,212,320 (GRCm39)	Q332R	probably benign	Het
Ugt2b1	T	C	5: 87,065,572 (GRCm39)	Y489C	probably damaging	Het
Ugt2b5	T	A	5: 87,275,631 (GRCm39)	M407L	probably benign	Het
Vmn1r191	A	T	13: 22,362,985 (GRCm39)	S256R	possibly damaging	Het
Vps13b	T	G	15: 35,925,554 (GRCm39)		probably null	Het
Vps13d	A	G	4: 144,882,713 (GRCm39)	F960S	probably damaging	Het
Xab2	T	A	8: 3,666,094 (GRCm39)	D227V	probably damaging	Het
Xaf1	A	T	11: 72,197,432 (GRCm39)	D136V	possibly damaging	Het
Zan	A	G	5: 137,387,545 (GRCm39)	S4889P	unknown	Het
Zfp78	A	G	7: 6,381,558 (GRCm39)	T203A	probably benign	Het
Zfp821	T	C	8: 110,447,874 (GRCm39)	S72P	probably damaging	Het

Other mutations in Kmt2b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00580:Kmt2b	APN	7	30,285,938 (GRCm39)	unclassified	probably benign
IGL00821:Kmt2b	APN	7	30,270,038 (GRCm39)	missense	probably damaging	1.00
IGL00985:Kmt2b	APN	7	30,279,352 (GRCm39)	missense	probably damaging	1.00
IGL01092:Kmt2b	APN	7	30,279,932 (GRCm39)	missense	probably damaging	1.00
IGL01933:Kmt2b	APN	7	30,268,939 (GRCm39)	critical splice donor site	probably null
IGL01949:Kmt2b	APN	7	30,276,586 (GRCm39)	splice site	probably null
IGL02253:Kmt2b	APN	7	30,281,152 (GRCm39)	missense	probably damaging	1.00
IGL02455:Kmt2b	APN	7	30,278,303 (GRCm39)	critical splice donor site	probably null
IGL02493:Kmt2b	APN	7	30,268,936 (GRCm39)	unclassified	probably benign
IGL02504:Kmt2b	APN	7	30,285,968 (GRCm39)	unclassified	probably benign
IGL02532:Kmt2b	APN	7	30,286,314 (GRCm39)	unclassified	probably benign
IGL02698:Kmt2b	APN	7	30,278,118 (GRCm39)	splice site	probably benign
IGL02717:Kmt2b	APN	7	30,282,869 (GRCm39)	missense	probably damaging	1.00
IGL02826:Kmt2b	APN	7	30,276,569 (GRCm39)	missense	probably damaging	1.00
IGL02966:Kmt2b	APN	7	30,274,887 (GRCm39)	missense	probably benign	0.02
IGL03386:Kmt2b	APN	7	30,273,396 (GRCm39)	missense	possibly damaging	0.94
Dean	UTSW	7	30,268,835 (GRCm39)	missense	possibly damaging	0.83
provost	UTSW	7	30,281,633 (GRCm39)	missense	probably damaging	1.00
tenure	UTSW	7	30,268,600 (GRCm39)	missense	probably damaging	1.00
3-1:Kmt2b	UTSW	7	30,269,040 (GRCm39)	nonsense	probably null
FR4304:Kmt2b	UTSW	7	30,285,788 (GRCm39)	unclassified	probably benign
FR4340:Kmt2b	UTSW	7	30,285,800 (GRCm39)	unclassified	probably benign
FR4340:Kmt2b	UTSW	7	30,285,794 (GRCm39)	unclassified	probably benign
FR4340:Kmt2b	UTSW	7	30,285,788 (GRCm39)	unclassified	probably benign
FR4342:Kmt2b	UTSW	7	30,285,800 (GRCm39)	unclassified	probably benign
FR4449:Kmt2b	UTSW	7	30,285,794 (GRCm39)	unclassified	probably benign
FR4449:Kmt2b	UTSW	7	30,285,791 (GRCm39)	unclassified	probably benign
FR4449:Kmt2b	UTSW	7	30,285,786 (GRCm39)	unclassified	probably benign
FR4548:Kmt2b	UTSW	7	30,285,805 (GRCm39)	unclassified	probably benign
FR4589:Kmt2b	UTSW	7	30,285,806 (GRCm39)	unclassified	probably benign
FR4589:Kmt2b	UTSW	7	30,285,789 (GRCm39)	nonsense	probably null
FR4589:Kmt2b	UTSW	7	30,285,786 (GRCm39)	unclassified	probably benign
FR4737:Kmt2b	UTSW	7	30,285,795 (GRCm39)	unclassified	probably benign
FR4737:Kmt2b	UTSW	7	30,285,792 (GRCm39)	unclassified	probably benign
FR4737:Kmt2b	UTSW	7	30,285,791 (GRCm39)	unclassified	probably benign
FR4737:Kmt2b	UTSW	7	30,285,803 (GRCm39)	unclassified	probably benign
FR4976:Kmt2b	UTSW	7	30,285,787 (GRCm39)	unclassified	probably benign
FR4976:Kmt2b	UTSW	7	30,285,785 (GRCm39)	unclassified	probably benign
FR4976:Kmt2b	UTSW	7	30,285,798 (GRCm39)	unclassified	probably benign
FR4976:Kmt2b	UTSW	7	30,285,791 (GRCm39)	unclassified	probably benign
FR4976:Kmt2b	UTSW	7	30,285,789 (GRCm39)	nonsense	probably null
PIT4403001:Kmt2b	UTSW	7	30,285,114 (GRCm39)	missense	probably damaging	1.00
PIT4802001:Kmt2b	UTSW	7	30,278,996 (GRCm39)	missense	probably damaging	0.99
R0057:Kmt2b	UTSW	7	30,276,217 (GRCm39)	splice site	probably benign
R0131:Kmt2b	UTSW	7	30,283,346 (GRCm39)	missense	probably damaging	0.99
R0241:Kmt2b	UTSW	7	30,276,494 (GRCm39)	missense	probably damaging	1.00
R0241:Kmt2b	UTSW	7	30,276,494 (GRCm39)	missense	probably damaging	1.00
R0377:Kmt2b	UTSW	7	30,273,618 (GRCm39)	missense	probably damaging	1.00
R0396:Kmt2b	UTSW	7	30,276,180 (GRCm39)	missense	probably damaging	1.00
R1241:Kmt2b	UTSW	7	30,274,365 (GRCm39)	missense	probably damaging	0.98
R1252:Kmt2b	UTSW	7	30,279,912 (GRCm39)	missense	probably damaging	0.99
R1418:Kmt2b	UTSW	7	30,276,385 (GRCm39)	splice site	probably benign
R1599:Kmt2b	UTSW	7	30,270,000 (GRCm39)	missense	probably damaging	1.00
R1632:Kmt2b	UTSW	7	30,283,387 (GRCm39)	missense	probably damaging	1.00
R1745:Kmt2b	UTSW	7	30,285,275 (GRCm39)	missense	possibly damaging	0.90
R1867:Kmt2b	UTSW	7	30,274,083 (GRCm39)	missense	possibly damaging	0.71
R2040:Kmt2b	UTSW	7	30,268,845 (GRCm39)	missense	probably damaging	1.00
R2113:Kmt2b	UTSW	7	30,282,812 (GRCm39)	missense	probably damaging	1.00
R2216:Kmt2b	UTSW	7	30,273,490 (GRCm39)	missense	probably benign	0.25
R2401:Kmt2b	UTSW	7	30,276,133 (GRCm39)	missense	probably damaging	1.00
R2518:Kmt2b	UTSW	7	30,275,493 (GRCm39)	missense	probably benign	0.10
R3436:Kmt2b	UTSW	7	30,276,117 (GRCm39)	missense	probably damaging	1.00
R4248:Kmt2b	UTSW	7	30,273,489 (GRCm39)	missense	probably benign	0.25
R4259:Kmt2b	UTSW	7	30,280,506 (GRCm39)	missense	probably damaging	0.99
R4290:Kmt2b	UTSW	7	30,281,261 (GRCm39)	critical splice donor site	probably null
R4388:Kmt2b	UTSW	7	30,288,015 (GRCm39)	unclassified	probably benign
R4542:Kmt2b	UTSW	7	30,279,684 (GRCm39)	missense	probably damaging	0.99
R4649:Kmt2b	UTSW	7	30,285,783 (GRCm39)	unclassified	probably benign
R4722:Kmt2b	UTSW	7	30,282,627 (GRCm39)	missense	probably damaging	1.00
R4891:Kmt2b	UTSW	7	30,276,186 (GRCm39)	nonsense	probably null
R4916:Kmt2b	UTSW	7	30,277,942 (GRCm39)	missense	probably damaging	0.99
R5104:Kmt2b	UTSW	7	30,269,265 (GRCm39)	missense	probably damaging	1.00
R5254:Kmt2b	UTSW	7	30,268,600 (GRCm39)	missense	probably damaging	1.00
R5262:Kmt2b	UTSW	7	30,269,219 (GRCm39)	missense	probably damaging	1.00
R5307:Kmt2b	UTSW	7	30,281,098 (GRCm39)	missense	possibly damaging	0.91
R5526:Kmt2b	UTSW	7	30,279,869 (GRCm39)	missense	probably damaging	1.00
R5609:Kmt2b	UTSW	7	30,276,570 (GRCm39)	missense	probably damaging	0.99
R6150:Kmt2b	UTSW	7	30,287,902 (GRCm39)	unclassified	probably benign
R6727:Kmt2b	UTSW	7	30,283,984 (GRCm39)	missense	probably damaging	0.98
R6824:Kmt2b	UTSW	7	30,285,701 (GRCm39)	unclassified	probably benign
R7048:Kmt2b	UTSW	7	30,268,731 (GRCm39)	missense	probably damaging	0.99
R7155:Kmt2b	UTSW	7	30,279,388 (GRCm39)	missense	probably damaging	0.99
R7307:Kmt2b	UTSW	7	30,279,896 (GRCm39)	missense	probably damaging	0.99
R7388:Kmt2b	UTSW	7	30,281,385 (GRCm39)	missense	probably damaging	1.00
R7555:Kmt2b	UTSW	7	30,268,835 (GRCm39)	missense	possibly damaging	0.83
R7569:Kmt2b	UTSW	7	30,268,978 (GRCm39)	missense	possibly damaging	0.54
R7616:Kmt2b	UTSW	7	30,281,633 (GRCm39)	missense	probably damaging	1.00
R7669:Kmt2b	UTSW	7	30,282,656 (GRCm39)	missense	possibly damaging	0.84
R7881:Kmt2b	UTSW	7	30,279,208 (GRCm39)	missense	probably damaging	1.00
R7999:Kmt2b	UTSW	7	30,276,199 (GRCm39)	missense	probably damaging	1.00
R8003:Kmt2b	UTSW	7	30,268,802 (GRCm39)	missense	probably damaging	0.98
R8189:Kmt2b	UTSW	7	30,268,756 (GRCm39)	missense	probably damaging	0.98
R8291:Kmt2b	UTSW	7	30,284,894 (GRCm39)	missense	probably damaging	1.00
R8314:Kmt2b	UTSW	7	30,278,347 (GRCm39)	missense	probably damaging	0.99
R8802:Kmt2b	UTSW	7	30,283,496 (GRCm39)	missense	probably damaging	1.00
R8954:Kmt2b	UTSW	7	30,273,640 (GRCm39)	missense	probably damaging	1.00
R9046:Kmt2b	UTSW	7	30,285,479 (GRCm39)	missense	probably benign	0.00
R9225:Kmt2b	UTSW	7	30,286,172 (GRCm39)	missense	unknown
R9258:Kmt2b	UTSW	7	30,281,893 (GRCm39)	missense	probably null	0.99
R9414:Kmt2b	UTSW	7	30,282,307 (GRCm39)	missense	probably damaging	0.99
R9468:Kmt2b	UTSW	7	30,284,513 (GRCm39)	missense	probably damaging	0.98
R9508:Kmt2b	UTSW	7	30,269,259 (GRCm39)	missense	probably damaging	0.99
R9642:Kmt2b	UTSW	7	30,283,340 (GRCm39)	critical splice donor site	probably null
R9667:Kmt2b	UTSW	7	30,287,784 (GRCm39)	missense	unknown
R9709:Kmt2b	UTSW	7	30,279,228 (GRCm39)	missense	probably damaging	0.98
RF001:Kmt2b	UTSW	7	30,285,807 (GRCm39)	unclassified	probably benign
RF006:Kmt2b	UTSW	7	30,285,802 (GRCm39)	unclassified	probably benign
RF020:Kmt2b	UTSW	7	30,285,807 (GRCm39)	unclassified	probably benign
RF021:Kmt2b	UTSW	7	30,285,782 (GRCm39)	unclassified	probably benign
RF030:Kmt2b	UTSW	7	30,285,802 (GRCm39)	unclassified	probably benign
RF035:Kmt2b	UTSW	7	30,285,782 (GRCm39)	unclassified	probably benign
X0067:Kmt2b	UTSW	7	30,278,998 (GRCm39)	missense	probably damaging	0.99
Z1088:Kmt2b	UTSW	7	30,284,676 (GRCm39)	missense	probably benign	0.28
Z1176:Kmt2b	UTSW	7	30,276,795 (GRCm39)	missense	probably damaging	1.00
Z1177:Kmt2b	UTSW	7	30,285,841 (GRCm39)	missense	unknown
Z1177:Kmt2b	UTSW	7	30,283,588 (GRCm39)	missense	probably damaging	0.98
Z1177:Kmt2b	UTSW	7	30,274,449 (GRCm39)	missense	probably benign	0.08
Z1186:Kmt2b	UTSW	7	30,284,732 (GRCm39)	missense	probably benign
Z1186:Kmt2b	UTSW	7	30,274,404 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGAAGGCAGGTCTTCAGGAG -3'
(R):5'- AGACTCGGACTTTCCAGCTC -3'

Sequencing Primer
(F):5'- TTGTTGGACAGCCTCAGC -3'
(R):5'- GGACTTTCCAGCTCCCCCAAG -3'

Posted On

2014-08-01