Mutagenetix > Incidental Mutations

Incidental Mutation 'R1957:Olfr887'

217928

Institutional Source

Beutler Lab

Gene Symbol

Olfr887

Ensembl Gene

ENSMUSG00000096167

Gene Name

olfactory receptor 887

Synonyms

MOR162-5, GA_x6K02T2PVTD-31764095-31765024

MMRRC Submission

039971-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R1957 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38084072-38090737 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 38085123 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 96 (C96S)

Ref Sequence

ENSEMBL: ENSMUSP00000148346 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074681] [ENSMUST00000212502] [ENSMUST00000213091]

Predicted Effect

probably damaging
Transcript: ENSMUST00000074681
AA Change: C96S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000074248
Gene: ENSMUSG00000096167
AA Change: C96S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	3.7e-49	PFAM
Pfam:7TM_GPCR_Srsx	35	253	3.1e-5	PFAM
Pfam:7tm_1	41	288	8.2e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000212502
AA Change: C96S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000213091
AA Change: C96S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6383

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.4%

Validation Efficiency

97% (117/121)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 114 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aaas	C	T	15: 102,338,633		probably benign	Het
Abce1	A	G	8: 79,685,949	I583T	probably benign	Het
Adcy1	A	T	11: 7,161,945	T937S	probably benign	Het
Adgra2	A	G	8: 27,111,168	I279V	possibly damaging	Het
Adgrl4	A	T	3: 151,510,779	N533I	possibly damaging	Het
Adipor1	T	C	1: 134,423,033	S7P	probably benign	Het
Amelx	A	T	X: 169,182,157		probably null	Het
Anks1b	A	T	10: 90,049,930	T163S	probably damaging	Het
Apc	A	G	18: 34,317,335	E2394G	probably damaging	Het
Arhgef39	C	T	4: 43,499,309	G56E	probably damaging	Het
Arnt	C	T	3: 95,448,393	S16L	possibly damaging	Het
Arsj	A	G	3: 126,439,021	N472S	probably benign	Het
Atg2b	T	C	12: 105,669,418	Y197C	probably damaging	Het
B3gat1	A	T	9: 26,755,952	D160V	possibly damaging	Het
Bmp7	T	C	2: 172,939,921	E50G	probably damaging	Het
Bms1	T	C	6: 118,392,978	E869G	probably damaging	Het
Brd4	G	A	17: 32,221,366	P332L	possibly damaging	Het
Btbd11	T	C	10: 85,633,699	L828P	probably damaging	Het
C130074G19Rik	T	C	1: 184,882,898	T32A	probably benign	Het
C1rl	A	T	6: 124,509,062	Y464F	probably damaging	Het
Ccdc150	T	A	1: 54,263,909	M193K	probably benign	Het
Ccdc7a	A	G	8: 128,980,135	S338P	probably damaging	Het
Cenpu	T	C	8: 46,572,837		probably benign	Het
Cep170	A	C	1: 176,769,447	V448G	probably benign	Het
Col27a1	G	T	4: 63,277,794	A879S	probably benign	Het
Crtac1	C	T	19: 42,287,944	S515N	possibly damaging	Het
Cstad	G	A	2: 30,608,281	V43M	unknown	Het
Daam1	T	C	12: 71,982,755		probably null	Het
Dnmt1	C	T	9: 20,927,146	R207H	probably benign	Het
Dsg3	A	G	18: 20,522,105	N153S	probably damaging	Het
Dzip3	C	A	16: 48,927,593	L1151F	probably damaging	Het
Eml5	A	T	12: 98,859,961	H644Q	probably damaging	Het
Emsy	A	G	7: 98,647,820	L52P	probably damaging	Het
Eno1	T	A	4: 150,246,775		probably null	Het
Epha3	T	A	16: 63,772,952	T258S	probably benign	Het
Ern1	T	C	11: 106,426,897	T134A	probably damaging	Het
Fam151b	G	T	13: 92,477,902	T26K	probably damaging	Het
Fam151b	T	A	13: 92,477,903	T26S	probably damaging	Het
Fat1	T	C	8: 45,040,682	V3955A	probably damaging	Het
Fbn1	T	C	2: 125,367,654	N930S	possibly damaging	Het
Fgr	A	G	4: 132,998,362	M361V	probably benign	Het
Fmo4	G	A	1: 162,803,690	T236I	probably benign	Het
Fndc7	G	A	3: 108,883,509	T67I	probably damaging	Het
Fxr2	A	G	11: 69,643,940	T216A	probably benign	Het
Gdf10	G	A	14: 33,932,753	A406T	probably benign	Het
Gm42669	A	T	5: 107,508,872	E355D	possibly damaging	Het
Gm438	G	A	4: 144,777,819	T254I	possibly damaging	Het
Gm5174	A	T	10: 86,656,753		noncoding transcript	Het
Gm5431	A	T	11: 48,888,397	L844*	probably null	Het
Gm6489	T	C	1: 31,287,371		noncoding transcript	Het
Gm9833	G	T	3: 10,089,286	V372F	probably benign	Het
Gna11	A	G	10: 81,530,844	V344A	probably damaging	Het
Gtpbp3	A	G	8: 71,490,455	E170G	probably damaging	Het
H2afz	A	C	3: 137,865,514		probably benign	Het
Heatr1	A	G	13: 12,396,538	N87D	probably damaging	Het
Iars2	T	C	1: 185,295,671	K687E	possibly damaging	Het
Ica1	T	C	6: 8,749,736	D71G	possibly damaging	Het
Ifnlr1	T	C	4: 135,686,570	L10P	probably damaging	Het
Il1r1	T	A	1: 40,313,140	L490*	probably null	Het
Ip6k3	A	T	17: 27,151,168	L92Q	probably benign	Het
Itgbl1	T	A	14: 123,966,678	F394I	probably damaging	Het
Kat6b	T	C	14: 21,628,879	Y437H	probably damaging	Het
Kcnh5	G	C	12: 74,897,584	Q964E	probably benign	Het
Krt27	A	T	11: 99,346,483		probably null	Het
Mki67	A	T	7: 135,698,399	D1635E	probably benign	Het
Mmp23	G	T	4: 155,652,052	H177Q	possibly damaging	Het
Mylk2	C	A	2: 152,917,607	Q406K	possibly damaging	Het
Myo1g	A	G	11: 6,512,159		probably null	Het
Myrf	T	C	19: 10,219,796	T261A	probably benign	Het
Nmt2	C	T	2: 3,325,382	P486L	possibly damaging	Het
Oard1	T	A	17: 48,415,276	L100*	probably null	Het
Oca2	T	C	7: 56,321,498	I391T	possibly damaging	Het
Olfr181	T	A	16: 58,926,167	M135L	probably benign	Het
Olfr488	A	C	7: 108,255,196	L314*	probably null	Het
Olfr620	T	A	7: 103,611,411	*314L	probably null	Het
Olfr780	A	T	10: 129,321,871	I83F	possibly damaging	Het
Olfr826	C	A	10: 130,179,978	A301S	possibly damaging	Het
Pabpc4	T	A	4: 123,286,865	S127T	probably damaging	Het
Pcdhb1	A	G	18: 37,265,707	D237G	probably damaging	Het
Pdcd6ip	T	C	9: 113,708,022	Y29C	probably damaging	Het
Phf3	C	A	1: 30,831,520	R95L	probably damaging	Het
Pkd1l2	A	T	8: 117,030,682	V1539D	probably damaging	Het
Plxna1	A	T	6: 89,331,291	D1271E	probably damaging	Het
Ppp3r2	A	G	4: 49,681,726	F75L	probably damaging	Het
Pth2r	C	A	1: 65,372,355	D350E	probably damaging	Het
Rabgap1	T	C	2: 37,483,762	F262S	possibly damaging	Het
Rbbp6	T	C	7: 122,990,288	S438P	probably benign	Het
Rexo1	G	A	10: 80,543,366	R1038C	probably damaging	Het
Rnf10	A	G	5: 115,260,322		probably benign	Het
Scyl1	C	A	19: 5,760,104	A565S	probably benign	Het
Sept4	A	G	11: 87,590,367	T378A	probably benign	Het
Sh3yl1	A	G	12: 30,942,788		probably null	Het
Slc1a7	G	T	4: 107,968,585	D14Y	probably benign	Het
Slc32a1	T	C	2: 158,614,043	V206A	probably damaging	Het
Smyd5	G	A	6: 85,438,139	R43Q	probably benign	Het
Snrnp200	G	A	2: 127,216,175	A286T	possibly damaging	Het
Sp110	A	C	1: 85,577,329	F434C	probably benign	Het
Srebf2	T	A	15: 82,194,954	H794Q	probably benign	Het
Ssbp2	T	C	13: 91,664,184		probably benign	Het
Sspo	G	A	6: 48,478,273	G3023D	probably damaging	Het
St6galnac5	T	A	3: 152,846,483	Q149L	probably benign	Het
Stab2	A	G	10: 86,861,470	Y1985H	probably benign	Het
Suz12	A	G	11: 79,999,100	M146V	probably benign	Het
Tac2	A	G	10: 127,728,480		probably null	Het
Thbd	T	C	2: 148,406,979	E323G	probably damaging	Het
Tnfrsf26	T	A	7: 143,617,923	T98S	probably damaging	Het
Trim15	T	C	17: 36,862,323		probably benign	Het
Trpt1	T	C	19: 6,998,193	V105A	possibly damaging	Het
Vcam1	T	C	3: 116,125,957	Y226C	probably damaging	Het
Vmn1r175	A	G	7: 23,808,383	V273A	probably benign	Het
Vmn2r109	A	G	17: 20,564,707	V17A	probably benign	Het
Vmn2r84	G	A	10: 130,390,808	A387V	probably benign	Het
Wdfy4	A	T	14: 32,971,684	L2728Q	probably damaging	Het
Zfp934	T	A	13: 62,518,294	T178S	possibly damaging	Het

Other mutations in Olfr887

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02506:Olfr887	APN	9	38085445	missense	probably damaging	1.00
R0639:Olfr887	UTSW	9	38085370	missense	probably damaging	1.00
R0671:Olfr887	UTSW	9	38085127	missense	possibly damaging	0.91
R1958:Olfr887	UTSW	9	38085123	missense	probably damaging	1.00
R2126:Olfr887	UTSW	9	38085276	missense	probably benign	0.02
R5329:Olfr887	UTSW	9	38085126	missense	probably benign	0.00
R5541:Olfr887	UTSW	9	38085123	missense	probably damaging	1.00
R5681:Olfr887	UTSW	9	38085631	missense	possibly damaging	0.90
R6042:Olfr887	UTSW	9	38085094	missense	probably damaging	0.99
R6417:Olfr887	UTSW	9	38085594	missense	probably benign	0.18
R6420:Olfr887	UTSW	9	38085594	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- CTTGGGAATCTATGTGGTCTCC -3'
(R):5'- TCTCAGCATGCAAATGATGTGTG -3'

Sequencing Primer
(F):5'- AATCTATGTGGTCTCCATTGTGG -3'
(R):5'- TGCCACAGCACCCACAG -3'

Posted On

2014-08-01