Mutagenetix > Incidental Mutation

Incidental Mutation 'R1959:Pcsk5'

218231

Institutional Source

Beutler Lab

Gene Symbol

Pcsk5

Ensembl Gene

ENSMUSG00000024713

Gene Name

proprotein convertase subtilisin/kexin type 5

Synonyms

PC6, SPC6, b2b1549Clo, b2b585Clo, PC5A, PC5/6A

MMRRC Submission

039973-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1959 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

17432832-17837632 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 17433418 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1870 (D1870G)

Ref Sequence

ENSEMBL: ENSMUSP00000025618 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025618]

AlphaFold

Q04592

Predicted Effect

unknown
Transcript: ENSMUST00000025618
AA Change: D1870G

SMART Domains

Protein: ENSMUSP00000025618
Gene: ENSMUSG00000024713
AA Change: D1870G

Domain	Start	End	E-Value	Type
low complexity region	17	29	N/A	INTRINSIC
Pfam:S8_pro-domain	40	116	4.6e-27	PFAM
Pfam:Peptidase_S8	164	447	2.1e-46	PFAM
Pfam:P_proprotein	507	597	2.1e-33	PFAM
FU	632	682	4.92e-13	SMART
FU	685	732	4.84e-12	SMART
EGF_like	690	723	3.29e1	SMART
FU	736	779	1.29e-7	SMART
FU	781	826	5.74e-14	SMART
FU	834	881	2.23e-11	SMART
EGF_like	839	870	3.43e1	SMART
FU	884	929	1.84e-12	SMART
FU	931	981	1.47e-11	SMART
FU	984	1030	1e-4	SMART
EGF_like	989	1020	2.92e1	SMART
FU	1034	1079	5.04e-10	SMART
FU	1081	1123	3.08e-5	SMART
FU	1127	1168	4.88e-8	SMART
FU	1206	1248	2.7e-10	SMART
EGF_like	1211	1239	5.91e1	SMART
FU	1252	1299	1.48e-7	SMART
EGF	1264	1305	1.69e1	SMART
FU	1301	1345	2.31e-9	SMART
FU	1347	1390	8.98e-7	SMART
EGF_like	1352	1381	7.23e1	SMART
FU	1392	1438	1.04e-11	SMART
FU	1442	1487	6.8e-7	SMART
EGF	1447	1476	2.16e1	SMART
FU	1491	1536	3.37e-11	SMART
FU	1540	1585	9.32e-14	SMART
EGF_like	1545	1576	2.8e1	SMART
FU	1589	1636	1.39e-12	SMART
FU	1640	1685	6.49e-13	SMART
EGF_like	1645	1676	6.67e1	SMART
FU	1691	1738	7.01e-9	SMART
transmembrane domain	1770	1789	N/A	INTRINSIC
low complexity region	1827	1840	N/A	INTRINSIC
low complexity region	1858	1876	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
PHENOTYPE: Mice homozygous for a null mutation in this gene display embryonic lethality between E4.5-E7.5. Mice homozygous for ENU-induced mutations exhibit heterotaxia with congenital heart defects and immotile respiratory cilia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 130 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123L14Rik	A	G	6: 96,165,269 (GRCm38)	S265P	possibly damaging	Het
Abcc1	T	C	16: 14,396,393 (GRCm38)	Y191H	probably damaging	Het
Aco1	T	C	4: 40,167,193 (GRCm38)		probably null	Het
Adap1	A	G	5: 139,273,341 (GRCm38)	Y364H	probably benign	Het
Add2	T	C	6: 86,096,756 (GRCm38)	F209S	probably damaging	Het
Adgb	A	T	10: 10,395,249 (GRCm38)	D883E	probably benign	Het
Als2cr12	A	T	1: 58,659,278 (GRCm38)	V327D	possibly damaging	Het
Anapc1	C	A	2: 128,633,415 (GRCm38)	R1381S	probably benign	Het
Aoah	A	G	13: 20,794,394 (GRCm38)	M1V	probably null	Het
Arap1	C	A	7: 101,373,015 (GRCm38)	A8E	probably damaging	Het
Arhgap10	A	G	8: 77,409,626 (GRCm38)	F319S	possibly damaging	Het
Btrc	T	G	19: 45,527,343 (GRCm38)	I480S	probably damaging	Het
Cabin1	A	T	10: 75,735,090 (GRCm38)	V784E	possibly damaging	Het
Card9	T	A	2: 26,354,873 (GRCm38)		probably null	Het
Cdk18	A	G	1: 132,117,821 (GRCm38)	I238T	possibly damaging	Het
Clec12a	A	C	6: 129,350,481 (GRCm38)	T21P	possibly damaging	Het
Commd3	A	T	2: 18,673,963 (GRCm38)	I70F	probably benign	Het
Cspg5	G	A	9: 110,251,026 (GRCm38)	V340M	probably damaging	Het
Cyb5r4	G	A	9: 87,055,849 (GRCm38)	S307N	possibly damaging	Het
Cyp26c1	T	A	19: 37,687,377 (GRCm38)	F230I	probably damaging	Het
Ddx11	A	G	17: 66,130,728 (GRCm38)	M150V	probably benign	Het
Dennd4b	A	G	3: 90,268,773 (GRCm38)	Y190C	probably damaging	Het
Det1	A	G	7: 78,843,443 (GRCm38)	V271A	probably benign	Het
Dgkd	C	A	1: 87,929,827 (GRCm38)	P754T	possibly damaging	Het
Dhx36	T	C	3: 62,479,385 (GRCm38)	S649G	probably benign	Het
Dlgap5	A	G	14: 47,416,386 (GRCm38)	I62T	possibly damaging	Het
Dmgdh	A	G	13: 93,720,559 (GRCm38)	M724V	probably benign	Het
Dnah7a	A	G	1: 53,684,983 (GRCm38)	S108P	probably benign	Het
Dock4	A	T	12: 40,710,798 (GRCm38)	K495M	probably damaging	Het
Dse	A	G	10: 34,160,206 (GRCm38)	Y225H	probably damaging	Het
Emx1	G	A	6: 85,203,934 (GRCm38)	R211K	probably damaging	Het
Ergic2	A	G	6: 148,199,354 (GRCm38)		probably null	Het
Fbxo27	G	A	7: 28,698,372 (GRCm38)	C277Y	possibly damaging	Het
Fcrl1	T	C	3: 87,376,520 (GRCm38)	I9T	possibly damaging	Het
Fjx1	T	C	2: 102,450,807 (GRCm38)	E261G	probably benign	Het
Flnb	C	T	14: 7,884,735 (GRCm38)	Q445*	probably null	Het
Flrt2	G	A	12: 95,780,300 (GRCm38)	V471I	probably benign	Het
Frmd4a	G	A	2: 4,535,186 (GRCm38)	V210M	probably damaging	Het
Fsip2	A	G	2: 82,991,550 (GRCm38)	K5876E	probably benign	Het
Galnt10	T	C	11: 57,765,617 (GRCm38)	L209P	probably damaging	Het
Gata5	A	T	2: 180,326,936 (GRCm38)	S382T	possibly damaging	Het
Glt6d1	C	A	2: 25,794,413 (GRCm38)	V194L	probably damaging	Het
Gm10803	T	G	2: 93,563,943 (GRCm38)	V20G	unknown	Het
Gm44511	T	G	6: 128,820,271 (GRCm38)	T52P	probably damaging	Het
Gpat4	A	T	8: 23,182,936 (GRCm38)	L88Q	possibly damaging	Het
Gpr15	T	A	16: 58,718,007 (GRCm38)	I240L	probably benign	Het
Hivep2	A	T	10: 14,132,709 (GRCm38)	I1684F	probably benign	Het
Hmcn1	T	C	1: 150,649,676 (GRCm38)	T3366A	probably benign	Het
Hnmt	A	G	2: 24,003,882 (GRCm38)	V200A	possibly damaging	Het
Hps6	A	T	19: 46,004,335 (GRCm38)	H237L	probably benign	Het
Hspg2	C	T	4: 137,564,895 (GRCm38)	P4033S	probably damaging	Het
Irf9	T	A	14: 55,607,717 (GRCm38)	S297T	possibly damaging	Het
Kdm3b	T	C	18: 34,812,395 (GRCm38)	V753A	possibly damaging	Het
Kif21a	G	A	15: 90,970,848 (GRCm38)	A703V	probably damaging	Het
Kif27	T	G	13: 58,293,123 (GRCm38)	R1159S	probably benign	Het
Krtap4-16	A	G	11: 99,851,547 (GRCm38)	V9A	unknown	Het
Lama2	G	T	10: 27,422,618 (GRCm38)	P161T	probably damaging	Het
Ltbp4	G	A	7: 27,329,018 (GRCm38)	P273L	unknown	Het
Lvrn	T	A	18: 46,894,717 (GRCm38)	S866R	probably damaging	Het
Med13	A	G	11: 86,298,979 (GRCm38)	Y1035H	probably damaging	Het
Mertk	T	A	2: 128,759,090 (GRCm38)	N331K	probably damaging	Het
Mios	T	A	6: 8,215,437 (GRCm38)	F211Y	probably benign	Het
Mpeg1	G	A	19: 12,462,911 (GRCm38)	V578M	probably damaging	Het
Mphosph9	A	T	5: 124,315,701 (GRCm38)	S183T	possibly damaging	Het
Mrto4	A	T	4: 139,349,638 (GRCm38)	I56N	probably damaging	Het
Muc5b	T	C	7: 141,862,637 (GRCm38)	C3107R	possibly damaging	Het
Ncoa2	A	G	1: 13,160,252 (GRCm38)	Y1023H	probably damaging	Het
Nlrp2	T	C	7: 5,327,738 (GRCm38)	E553G	probably damaging	Het
Nlrp6	GAGAAGAAGAAGAAGAAGAAGA	GAGAAGAAGAAGAAGAAGA	7: 140,924,113 (GRCm38)		probably benign	Het
Nr2f1	T	C	13: 78,189,816 (GRCm38)	T237A	probably damaging	Het
Nup205	C	A	6: 35,233,366 (GRCm38)	Q1621K	probably benign	Het
Nxpe2	T	A	9: 48,319,726 (GRCm38)	S448C	probably benign	Het
Ogdh	T	A	11: 6,346,638 (GRCm38)	C498S	possibly damaging	Het
Olfr1176	T	C	2: 88,340,201 (GRCm38)	L212P	probably damaging	Het
Olfr281	T	C	15: 98,456,753 (GRCm38)	S148P	probably damaging	Het
Olfr294	A	T	7: 86,616,431 (GRCm38)	F71L	probably benign	Het
Olfr414	A	T	1: 174,430,905 (GRCm38)	K159M	probably damaging	Het
Olfr697	T	C	7: 106,741,394 (GRCm38)	E180G	probably damaging	Het
Olfr715	A	T	7: 107,128,510 (GRCm38)	D294E	possibly damaging	Het
Olfr994	T	C	2: 85,430,619 (GRCm38)	D70G	probably damaging	Het
Oplah	G	A	15: 76,297,464 (GRCm38)	T1119I	probably damaging	Het
Pcdhb9	T	A	18: 37,403,316 (GRCm38)	Y788N	probably damaging	Het
Pde6g	A	G	11: 120,448,136 (GRCm38)	L76P	probably damaging	Het
Peak1	A	T	9: 56,206,789 (GRCm38)	Y593N	probably damaging	Het
Pfas	C	T	11: 68,994,284 (GRCm38)	G16R	probably damaging	Het
Pkd1l2	A	T	8: 117,043,231 (GRCm38)		probably null	Het
Pla2g3	C	T	11: 3,490,983 (GRCm38)	T316I	probably benign	Het
Ptpru	T	A	4: 131,803,477 (GRCm38)	I489F	probably damaging	Het
Rere	T	G	4: 150,468,790 (GRCm38)	H146Q	probably benign	Het
Rundc1	A	T	11: 101,431,496 (GRCm38)	Q272L	probably damaging	Het
Scml4	T	C	10: 42,956,021 (GRCm38)	L305P	probably damaging	Het
Sec16a	A	T	2: 26,430,132 (GRCm38)	H1431Q	probably benign	Het
Serpina1a	G	A	12: 103,853,800 (GRCm38)	Q373*	probably null	Het
Shank1	A	T	7: 44,325,377 (GRCm38)	N377I	unknown	Het
Shc2	T	C	10: 79,626,791 (GRCm38)		probably null	Het
Slc22a29	C	A	19: 8,169,193 (GRCm38)	R415M	probably benign	Het
Slc7a2	A	T	8: 40,914,965 (GRCm38)	I589F	probably damaging	Het
Smim8	C	T	4: 34,771,316 (GRCm38)	R26Q	probably damaging	Het
Smox	C	A	2: 131,520,464 (GRCm38)	A221D	probably damaging	Het
Sox5	A	G	6: 143,874,105 (GRCm38)	S62P	possibly damaging	Het
Spg21	A	C	9: 65,484,492 (GRCm38)	K240N	probably damaging	Het
Sv2c	C	T	13: 95,976,645 (GRCm38)	V599M	probably damaging	Het
Tanc2	T	A	11: 105,910,295 (GRCm38)	H1112Q	probably damaging	Het
Tbata	T	C	10: 61,175,844 (GRCm38)	I58T	possibly damaging	Het
Tbc1d2	T	G	4: 46,606,419 (GRCm38)	Y842S	probably benign	Het
Tctn1	A	T	5: 122,241,840 (GRCm38)		probably null	Het
Tenm1	T	C	X: 42,827,201 (GRCm38)	D402G	probably benign	Het
Tfcp2l1	T	C	1: 118,669,389 (GRCm38)	V400A	probably benign	Het
Tm9sf2	T	A	14: 122,126,164 (GRCm38)	L99I	probably benign	Het
Top2a	T	C	11: 98,995,977 (GRCm38)		probably null	Het
Traf7	C	A	17: 24,513,281 (GRCm38)	G191C	probably damaging	Het
Trpm1	T	A	7: 64,230,230 (GRCm38)	L661Q	probably damaging	Het
Ttc30b	C	T	2: 75,937,099 (GRCm38)	E437K	probably benign	Het
Ttn	T	C	2: 76,750,623 (GRCm38)	I23309V	probably benign	Het
Usp50	T	C	2: 126,777,961 (GRCm38)	K199E	possibly damaging	Het
Vmn1r218	T	C	13: 23,136,513 (GRCm38)	F10S	probably damaging	Het
Vmn2r89	C	A	14: 51,457,440 (GRCm38)	T459K	probably benign	Het
Vps13a	T	G	19: 16,677,938 (GRCm38)	S1909R	possibly damaging	Het
Vwa5b2	T	C	16: 20,602,191 (GRCm38)		probably null	Het
Vwa8	A	G	14: 78,982,360 (GRCm38)	H516R	possibly damaging	Het
Wnk1	T	C	6: 119,969,247 (GRCm38)	I648M	probably damaging	Het
Zfat	G	C	15: 68,146,543 (GRCm38)	P974R	probably benign	Het
Zfc3h1	T	A	10: 115,423,253 (GRCm38)	I1601K	probably benign	Het
Zfp239	A	G	6: 117,871,817 (GRCm38)	K172R	probably benign	Het
Zfp335	C	T	2: 164,894,802 (GRCm38)	G971D	probably damaging	Het
Zfp532	A	T	18: 65,624,492 (GRCm38)	I499F	probably damaging	Het
Zfp647	T	C	15: 76,911,114 (GRCm38)	T449A	possibly damaging	Het
Zfp938	C	T	10: 82,225,631 (GRCm38)	G385D	probably damaging	Het
Zfp959	T	G	17: 55,897,404 (GRCm38)	V147G	probably damaging	Het
Znfx1	A	T	2: 167,050,350 (GRCm38)	C649S	probably damaging	Het

Other mutations in Pcsk5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Pcsk5	APN	19	17,511,421 (GRCm38)	missense	possibly damaging	0.49
IGL00423:Pcsk5	APN	19	17,642,559 (GRCm38)	missense	probably benign	0.23
IGL01315:Pcsk5	APN	19	17,451,958 (GRCm38)	missense	probably damaging	1.00
IGL01372:Pcsk5	APN	19	17,617,744 (GRCm38)	missense	probably damaging	1.00
IGL01738:Pcsk5	APN	19	17,433,780 (GRCm38)	splice site	probably benign
IGL01874:Pcsk5	APN	19	17,595,677 (GRCm38)	missense	probably damaging	0.96
IGL02070:Pcsk5	APN	19	17,439,042 (GRCm38)	missense	probably benign	0.25
IGL02311:Pcsk5	APN	19	17,433,420 (GRCm38)	nonsense	probably null
IGL02436:Pcsk5	APN	19	17,564,708 (GRCm38)	critical splice donor site	probably null
IGL02498:Pcsk5	APN	19	17,511,556 (GRCm38)	missense	probably damaging	0.99
IGL02504:Pcsk5	APN	19	17,477,872 (GRCm38)	critical splice donor site	probably null
IGL02664:Pcsk5	APN	19	17,456,770 (GRCm38)	missense	probably damaging	1.00
IGL02735:Pcsk5	APN	19	17,675,468 (GRCm38)	missense	probably damaging	1.00
IGL02941:Pcsk5	APN	19	17,447,501 (GRCm38)	missense	probably damaging	1.00
PIT4377001:Pcsk5	UTSW	19	17,439,102 (GRCm38)	missense	probably damaging	1.00
R0007:Pcsk5	UTSW	19	17,654,861 (GRCm38)	missense	probably damaging	1.00
R0007:Pcsk5	UTSW	19	17,654,861 (GRCm38)	missense	probably damaging	1.00
R0032:Pcsk5	UTSW	19	17,564,815 (GRCm38)	missense	possibly damaging	0.81
R0032:Pcsk5	UTSW	19	17,564,815 (GRCm38)	missense	possibly damaging	0.81
R0373:Pcsk5	UTSW	19	17,654,849 (GRCm38)	missense	probably damaging	1.00
R0784:Pcsk5	UTSW	19	17,714,769 (GRCm38)	missense	probably benign	0.06
R0843:Pcsk5	UTSW	19	17,654,818 (GRCm38)	missense	probably damaging	1.00
R1014:Pcsk5	UTSW	19	17,564,830 (GRCm38)	missense	probably damaging	1.00
R1221:Pcsk5	UTSW	19	17,837,148 (GRCm38)	missense	possibly damaging	0.85
R1435:Pcsk5	UTSW	19	17,563,882 (GRCm38)	nonsense	probably null
R1471:Pcsk5	UTSW	19	17,568,324 (GRCm38)	missense	probably damaging	1.00
R1564:Pcsk5	UTSW	19	17,654,756 (GRCm38)	missense	probably damaging	1.00
R1597:Pcsk5	UTSW	19	17,436,600 (GRCm38)	missense	probably benign	0.00
R1614:Pcsk5	UTSW	19	17,515,256 (GRCm38)	missense	probably damaging	1.00
R1661:Pcsk5	UTSW	19	17,447,574 (GRCm38)	missense	probably damaging	0.98
R1671:Pcsk5	UTSW	19	17,454,868 (GRCm38)	missense	probably damaging	1.00
R1703:Pcsk5	UTSW	19	17,752,094 (GRCm38)	missense	probably benign	0.15
R1793:Pcsk5	UTSW	19	17,454,750 (GRCm38)	missense	possibly damaging	0.83
R1855:Pcsk5	UTSW	19	17,515,192 (GRCm38)	missense	possibly damaging	0.93
R1909:Pcsk5	UTSW	19	17,433,461 (GRCm38)	missense	probably benign	0.00
R2006:Pcsk5	UTSW	19	17,477,916 (GRCm38)	missense	probably benign	0.32
R2045:Pcsk5	UTSW	19	17,581,144 (GRCm38)	missense	possibly damaging	0.48
R2061:Pcsk5	UTSW	19	17,454,872 (GRCm38)	missense	probably benign	0.03
R2110:Pcsk5	UTSW	19	17,473,059 (GRCm38)	missense	probably damaging	1.00
R2402:Pcsk5	UTSW	19	17,474,834 (GRCm38)	nonsense	probably null
R2496:Pcsk5	UTSW	19	17,466,158 (GRCm38)	nonsense	probably null
R4115:Pcsk5	UTSW	19	17,433,419 (GRCm38)	missense	unknown
R4504:Pcsk5	UTSW	19	17,451,955 (GRCm38)	missense	probably damaging	1.00
R4616:Pcsk5	UTSW	19	17,560,750 (GRCm38)	missense	probably benign	0.00
R4683:Pcsk5	UTSW	19	17,473,041 (GRCm38)	missense	probably damaging	1.00
R4717:Pcsk5	UTSW	19	17,525,267 (GRCm38)	missense	probably damaging	1.00
R4761:Pcsk5	UTSW	19	17,837,148 (GRCm38)	missense	possibly damaging	0.85
R4789:Pcsk5	UTSW	19	17,433,599 (GRCm38)	missense	probably benign	0.09
R4880:Pcsk5	UTSW	19	17,447,690 (GRCm38)	missense	probably damaging	1.00
R5100:Pcsk5	UTSW	19	17,515,135 (GRCm38)	critical splice donor site	probably null
R5114:Pcsk5	UTSW	19	17,675,585 (GRCm38)	missense	probably damaging	1.00
R5116:Pcsk5	UTSW	19	17,463,434 (GRCm38)	missense	possibly damaging	0.87
R5193:Pcsk5	UTSW	19	17,564,810 (GRCm38)	missense	possibly damaging	0.79
R5279:Pcsk5	UTSW	19	17,595,658 (GRCm38)	splice site	probably null
R5334:Pcsk5	UTSW	19	17,461,851 (GRCm38)	missense	probably benign	0.00
R5369:Pcsk5	UTSW	19	17,581,255 (GRCm38)	missense	probably damaging	1.00
R5451:Pcsk5	UTSW	19	17,463,356 (GRCm38)	missense	possibly damaging	0.91
R5547:Pcsk5	UTSW	19	17,752,124 (GRCm38)	missense	probably benign	0.08
R5630:Pcsk5	UTSW	19	17,575,831 (GRCm38)	missense	probably benign	0.04
R5805:Pcsk5	UTSW	19	17,456,829 (GRCm38)	missense	probably benign	0.01
R6063:Pcsk5	UTSW	19	17,454,681 (GRCm38)	critical splice donor site	probably null
R6130:Pcsk5	UTSW	19	17,511,556 (GRCm38)	missense	probably damaging	0.99
R6153:Pcsk5	UTSW	19	17,511,492 (GRCm38)	missense	probably damaging	0.98
R6163:Pcsk5	UTSW	19	17,473,041 (GRCm38)	missense	probably damaging	1.00
R6164:Pcsk5	UTSW	19	17,836,953 (GRCm38)	critical splice donor site	probably null
R6228:Pcsk5	UTSW	19	17,581,267 (GRCm38)	missense	possibly damaging	0.91
R6426:Pcsk5	UTSW	19	17,617,729 (GRCm38)	missense	probably damaging	1.00
R6601:Pcsk5	UTSW	19	17,511,380 (GRCm38)	missense	probably benign	0.00
R6648:Pcsk5	UTSW	19	17,575,821 (GRCm38)	missense	probably damaging	0.99
R6789:Pcsk5	UTSW	19	17,456,786 (GRCm38)	missense	possibly damaging	0.93
R6807:Pcsk5	UTSW	19	17,572,622 (GRCm38)	splice site	probably null
R6837:Pcsk5	UTSW	19	17,439,084 (GRCm38)	missense	probably benign	0.01
R6998:Pcsk5	UTSW	19	17,473,112 (GRCm38)	missense	probably benign	0.20
R7051:Pcsk5	UTSW	19	17,433,731 (GRCm38)	missense	probably benign	0.00
R7164:Pcsk5	UTSW	19	17,451,985 (GRCm38)	missense	probably damaging	1.00
R7173:Pcsk5	UTSW	19	17,477,877 (GRCm38)	missense	possibly damaging	0.85
R7348:Pcsk5	UTSW	19	17,456,818 (GRCm38)	nonsense	probably null
R7360:Pcsk5	UTSW	19	17,515,213 (GRCm38)	missense	probably benign	0.00
R7407:Pcsk5	UTSW	19	17,675,516 (GRCm38)	missense	probably damaging	1.00
R7447:Pcsk5	UTSW	19	17,510,236 (GRCm38)	missense	probably benign	0.31
R7521:Pcsk5	UTSW	19	17,454,832 (GRCm38)	missense	probably benign	0.29
R7525:Pcsk5	UTSW	19	17,642,590 (GRCm38)	missense	probably damaging	1.00
R7560:Pcsk5	UTSW	19	17,836,972 (GRCm38)	missense	probably benign	0.01
R7566:Pcsk5	UTSW	19	17,572,457 (GRCm38)	missense	probably benign
R7631:Pcsk5	UTSW	19	17,564,780 (GRCm38)	missense	probably damaging	1.00
R7654:Pcsk5	UTSW	19	17,456,804 (GRCm38)	missense	possibly damaging	0.46
R7677:Pcsk5	UTSW	19	17,581,229 (GRCm38)	missense	possibly damaging	0.59
R7711:Pcsk5	UTSW	19	17,439,080 (GRCm38)	missense	possibly damaging	0.82
R7903:Pcsk5	UTSW	19	17,572,483 (GRCm38)	missense	probably damaging	0.98
R7938:Pcsk5	UTSW	19	17,466,185 (GRCm38)	critical splice acceptor site	probably null
R8025:Pcsk5	UTSW	19	17,561,051 (GRCm38)	intron	probably benign
R8032:Pcsk5	UTSW	19	17,714,787 (GRCm38)	missense	probably damaging	0.98
R8064:Pcsk5	UTSW	19	17,714,861 (GRCm38)	missense	probably damaging	1.00
R8115:Pcsk5	UTSW	19	17,510,166 (GRCm38)	critical splice donor site	probably null
R8193:Pcsk5	UTSW	19	17,586,051 (GRCm38)	missense	possibly damaging	0.64
R8408:Pcsk5	UTSW	19	17,433,445 (GRCm38)	missense	probably benign	0.00
R8466:Pcsk5	UTSW	19	17,572,500 (GRCm38)	nonsense	probably null
R8739:Pcsk5	UTSW	19	17,454,774 (GRCm38)	missense	probably benign	0.00
R8753:Pcsk5	UTSW	19	17,469,044 (GRCm38)	missense	probably benign	0.00
R8797:Pcsk5	UTSW	19	17,466,108 (GRCm38)	missense	probably benign	0.00
R8944:Pcsk5	UTSW	19	17,474,911 (GRCm38)	missense	probably damaging	0.96
R9041:Pcsk5	UTSW	19	17,560,768 (GRCm38)	nonsense	probably null
R9135:Pcsk5	UTSW	19	17,586,108 (GRCm38)	missense
R9288:Pcsk5	UTSW	19	17,836,981 (GRCm38)	missense	probably benign	0.10
R9406:Pcsk5	UTSW	19	17,793,733 (GRCm38)	missense	probably benign	0.14
R9581:Pcsk5	UTSW	19	17,454,832 (GRCm38)	missense	probably benign
R9592:Pcsk5	UTSW	19	17,675,535 (GRCm38)	nonsense	probably null
R9659:Pcsk5	UTSW	19	17,477,881 (GRCm38)	missense	probably benign	0.00
R9788:Pcsk5	UTSW	19	17,477,881 (GRCm38)	missense	probably benign	0.00
X0023:Pcsk5	UTSW	19	17,474,872 (GRCm38)	missense	possibly damaging	0.66
X0063:Pcsk5	UTSW	19	17,447,604 (GRCm38)	missense	probably damaging	1.00
Z1088:Pcsk5	UTSW	19	17,463,374 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGATCTGTTCATTGCACTCAGC -3'
(R):5'- TCCTACAGGAGCAGCTATCTTG -3'

Sequencing Primer
(F):5'- AGCATCCCTACATATTCGGGTTGG -3'
(R):5'- CTATCTTGACGAGGACCAGGTG -3'

Posted On

2014-08-01