Mutagenetix > Incidental Mutation

Incidental Mutation 'R0720:Dynap'

218343

Institutional Source

Beutler Lab

Gene Symbol

Dynap

Ensembl Gene

ENSMUSG00000024512

Gene Name

dynactin associated protein

Synonyms

2310002L13Rik

MMRRC Submission

038902-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R0720 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

70373500-70377653 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 70374055 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 157 (D157G)

Ref Sequence

ENSEMBL: ENSMUSP00000025390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025390]

AlphaFold

Q9D7M5

Predicted Effect

unknown
Transcript: ENSMUST00000025390
AA Change: D157G

SMART Domains

Protein: ENSMUSP00000025390
Gene: ENSMUSG00000024512
AA Change: D157G

Domain	Start	End	E-Value	Type
Pfam:CLLAC	51	80	2.5e-16	PFAM
low complexity region	104	148	N/A	INTRINSIC

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.7%
20x: 95.9%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf2	A	G	17: 43,024,063 (GRCm39)	I136T	probably damaging	Het
Bbs7	T	C	3: 36,646,572 (GRCm39)	D416G	probably damaging	Het
Commd4	G	T	9: 57,062,718 (GRCm39)	D179E	probably benign	Het
Cyp3a57	T	C	5: 145,327,213 (GRCm39)		probably benign	Het
Dnah5	A	G	15: 28,314,007 (GRCm39)	N1941S	probably null	Het
Entrep2	A	G	7: 64,469,658 (GRCm39)		probably benign	Het
Eri3	T	C	4: 117,410,242 (GRCm39)		probably null	Het
Fbxo25	A	G	8: 13,985,222 (GRCm39)	Y305C	probably damaging	Het
Flt4	C	T	11: 49,527,166 (GRCm39)		probably benign	Het
Fxr2	A	G	11: 69,530,241 (GRCm39)	D36G	probably benign	Het
Gas2l3	T	C	10: 89,249,805 (GRCm39)	T438A	probably benign	Het
Gcm1	A	T	9: 77,971,923 (GRCm39)	Y288F	possibly damaging	Het
Gm3164	C	A	14: 4,442,719 (GRCm38)	S218R	probably benign	Het
Hipk2	C	T	6: 38,675,491 (GRCm39)	R1029H	probably damaging	Het
Htra3	T	C	5: 35,811,453 (GRCm39)	I392M	probably damaging	Het
Kansl1l	T	C	1: 66,840,515 (GRCm39)	M262V	possibly damaging	Het
Lrrc47	T	C	4: 154,104,344 (GRCm39)		probably null	Het
Macf1	A	T	4: 123,326,718 (GRCm39)	N4926K	probably damaging	Het
Mllt10	T	C	2: 18,201,406 (GRCm39)	S631P	probably benign	Het
Nlrp14	A	G	7: 106,781,220 (GRCm39)	H139R	probably benign	Het
Or1e34	T	A	11: 73,778,688 (GRCm39)	N170I	probably benign	Het
Or5w20	A	T	2: 87,727,013 (GRCm39)	T157S	probably benign	Het
Ptger2	T	C	14: 45,226,590 (GRCm39)	C57R	probably benign	Het
Rhot1	T	C	11: 80,114,769 (GRCm39)	V59A	probably damaging	Het
Rmdn2	G	A	17: 79,975,458 (GRCm39)		probably null	Het
Rxfp2	G	T	5: 149,967,584 (GRCm39)	K148N	probably benign	Het
Sec23a	G	A	12: 59,018,057 (GRCm39)	T623M	probably damaging	Het
Smcr8	T	C	11: 60,669,269 (GRCm39)	L139P	probably damaging	Het
Spag6l	A	T	16: 16,584,960 (GRCm39)		probably benign	Het
Taar1	T	C	10: 23,796,971 (GRCm39)	I223T	probably damaging	Het
Tdo2	G	T	3: 81,870,065 (GRCm39)	A269E	probably damaging	Het
Tnfsf18	A	G	1: 161,331,156 (GRCm39)	Y102C	possibly damaging	Het
Tns1	A	G	1: 73,964,740 (GRCm39)	L1297P	probably benign	Het
Txndc8	C	T	4: 57,984,245 (GRCm39)		probably benign	Het
Ubr5	T	C	15: 37,973,235 (GRCm39)	N2622S	probably damaging	Het
Vmn2r99	T	A	17: 19,599,305 (GRCm39)	F330I	probably benign	Het
Zdhhc2	T	A	8: 40,925,948 (GRCm39)		probably null	Het

Other mutations in Dynap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0041:Dynap	UTSW	18	70,375,105 (GRCm39)	missense	possibly damaging	0.73
R0528:Dynap	UTSW	18	70,375,165 (GRCm39)	splice site	probably benign
R2268:Dynap	UTSW	18	70,374,218 (GRCm39)	missense	probably benign	0.02
R4739:Dynap	UTSW	18	70,374,296 (GRCm39)	missense	possibly damaging	0.93
R5802:Dynap	UTSW	18	70,374,073 (GRCm39)	missense	unknown
R6841:Dynap	UTSW	18	70,374,253 (GRCm39)	missense	probably damaging	0.98
R7271:Dynap	UTSW	18	70,374,320 (GRCm39)	missense	possibly damaging	0.71
R7354:Dynap	UTSW	18	70,374,371 (GRCm39)	missense	possibly damaging	0.71
R8018:Dynap	UTSW	18	70,375,093 (GRCm39)	missense	possibly damaging	0.53
R8499:Dynap	UTSW	18	70,374,044 (GRCm39)	missense	unknown
R8706:Dynap	UTSW	18	70,374,062 (GRCm39)	missense	unknown
R8975:Dynap	UTSW	18	70,375,093 (GRCm39)	missense	possibly damaging	0.53
Z1177:Dynap	UTSW	18	70,374,101 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CACCACGTAGACCTGCATCTCTTG -3'
(R):5'- GCCCCTTTGGCAATTCCAACAATAC -3'

Sequencing Primer
(F):5'- GTCCTCTGACATGGAAAAAGTCTC -3'
(R):5'- GTTATTCACACAGATGGAAGGTCC -3'

Posted On

2014-08-01