Mutagenetix > Incidental Mutation

Incidental Mutation 'R0667:Or7g35'

218546

Institutional Source

Beutler Lab

Gene Symbol

Or7g35

Ensembl Gene

ENSMUSG00000043087

Gene Name

olfactory receptor family 7 subfamily G member 35

Synonyms

Olfr855, MOR148-1, GA_x6K02T2PVTD-13330461-13331399

MMRRC Submission

038852-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.132) question?

Stock #

R0667 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

19495807-19496798 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19496743 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 303 (N303K)

Ref Sequence

ENSEMBL: ENSMUSP00000150218 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061693] [ENSMUST00000215587]

AlphaFold

Q7TRF8

Predicted Effect

probably benign
Transcript: ENSMUST00000061693
AA Change: N303K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000054790
Gene: ENSMUSG00000043087
AA Change: N303K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	310	1.7e-54	PFAM
Pfam:7TM_GPCR_Srsx	37	182	5.2e-8	PFAM
Pfam:7tm_1	43	292	2.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215587
AA Change: N303K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 94.8%

Validation Efficiency

98% (64/65)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca5	G	T	11: 110,218,637 (GRCm39)	N76K	probably benign	Het
Adamts12	C	T	15: 11,215,710 (GRCm39)	R244C	probably damaging	Het
Atad2	A	G	15: 57,962,115 (GRCm39)	S1143P	probably benign	Het
Avl9	G	T	6: 56,713,468 (GRCm39)	R242L	probably benign	Het
Cand1	A	G	10: 119,052,425 (GRCm39)	S234P	probably benign	Het
Cd200	T	A	16: 45,215,220 (GRCm39)	I144L	probably benign	Het
Cep76	A	T	18: 67,767,848 (GRCm39)	L228Q	possibly damaging	Het
Col12a1	A	G	9: 79,535,744 (GRCm39)	L2584S	probably damaging	Het
Col6a3	A	C	1: 90,755,823 (GRCm39)	D155E	probably damaging	Het
Col6a4	G	A	9: 105,907,158 (GRCm39)		probably benign	Het
Dsg2	A	C	18: 20,706,556 (GRCm39)	D24A	possibly damaging	Het
Gm5901	G	A	7: 105,026,697 (GRCm39)	S155N	possibly damaging	Het
Hkdc1	T	C	10: 62,247,644 (GRCm39)		probably benign	Het
Kansl1	A	G	11: 104,234,364 (GRCm39)	V714A	probably benign	Het
Kcnh1	T	A	1: 192,188,346 (GRCm39)	S936T	probably benign	Het
Klhdc3	A	T	17: 46,988,151 (GRCm39)	F205I	probably benign	Het
Krt31	T	A	11: 99,938,951 (GRCm39)	H290L	probably benign	Het
Lama2	T	A	10: 27,220,406 (GRCm39)		probably null	Het
Mep1a	T	G	17: 43,789,081 (GRCm39)	D565A	probably benign	Het
Mgme1	T	A	2: 144,120,907 (GRCm39)		probably benign	Het
Mtf2	C	T	5: 108,252,369 (GRCm39)	T409I	probably damaging	Het
Mylk3	A	G	8: 86,081,794 (GRCm39)		probably null	Het
Myo1c	A	G	11: 75,559,338 (GRCm39)	E650G	probably damaging	Het
Nipbl	A	C	15: 8,390,488 (GRCm39)	D260E	possibly damaging	Het
Nufip2	T	A	11: 77,582,839 (GRCm39)	V251D	possibly damaging	Het
Or2n1e	C	A	17: 38,586,048 (GRCm39)	P129T	probably damaging	Het
Or4a2	T	C	2: 89,248,032 (GRCm39)	I242V	probably benign	Het
Osm	G	T	11: 4,189,918 (GRCm39)	R234L	possibly damaging	Het
Pabpc1	G	A	15: 36,598,275 (GRCm39)	A515V	probably benign	Het
Piwil1	T	A	5: 128,818,542 (GRCm39)		probably null	Het
Pld1	A	G	3: 28,133,327 (GRCm39)		probably null	Het
Plekhg3	C	T	12: 76,623,372 (GRCm39)	R871C	probably damaging	Het
Ppfia2	A	T	10: 106,749,555 (GRCm39)	Y1147F	probably damaging	Het
Prmt3	A	G	7: 49,441,743 (GRCm39)	Y240C	probably damaging	Het
Prr36	G	T	8: 4,266,311 (GRCm39)		probably benign	Het
Ptprd	A	G	4: 75,875,583 (GRCm39)	I908T	probably damaging	Het
Sae1	A	T	7: 16,102,457 (GRCm39)	N172K	probably damaging	Het
Satb1	T	G	17: 52,089,889 (GRCm39)	Q319H	probably damaging	Het
Scart2	C	G	7: 139,841,450 (GRCm39)	S251R	possibly damaging	Het
Scn2a	A	T	2: 65,582,340 (GRCm39)	I1563F	possibly damaging	Het
Scn3a	C	A	2: 65,314,755 (GRCm39)	R1102L	probably null	Het
Serpinb9b	T	A	13: 33,216,909 (GRCm39)	L60*	probably null	Het
Setd1a	A	G	7: 127,385,765 (GRCm39)	D281G	probably damaging	Het
Slc8a1	C	A	17: 81,956,310 (GRCm39)	V243F	probably damaging	Het
Tgfbr3	A	T	5: 107,325,716 (GRCm39)	H115Q	probably benign	Het
Tiam1	C	A	16: 89,694,872 (GRCm39)	S195I	probably damaging	Het
Tjp2	A	G	19: 24,086,113 (GRCm39)	V803A	probably benign	Het
Ttc5	T	A	14: 51,003,415 (GRCm39)	Q423L	probably benign	Het
Tyk2	A	C	9: 21,020,167 (GRCm39)	V997G	probably damaging	Het
Uhrf1	T	C	17: 56,617,677 (GRCm39)	V133A	probably benign	Het
Vmn2r107	A	C	17: 20,575,916 (GRCm39)	Y82S	possibly damaging	Het
Vmn2r93	T	C	17: 18,546,503 (GRCm39)	F792L	probably damaging	Het
Vps13c	G	A	9: 67,858,855 (GRCm39)	W2768*	probably null	Het
Zfp456	A	T	13: 67,514,861 (GRCm39)	C282S	probably benign	Het
Zhx1	T	C	15: 57,916,561 (GRCm39)	N562D	possibly damaging	Het
Zmynd15	G	T	11: 70,355,944 (GRCm39)	G481C	probably damaging	Het

Other mutations in Or7g35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Or7g35	APN	9	19,496,616 (GRCm39)	missense	possibly damaging	0.74
IGL01405:Or7g35	APN	9	19,496,501 (GRCm39)	missense	probably benign	0.23
IGL01775:Or7g35	APN	9	19,496,001 (GRCm39)	missense	probably benign	0.04
IGL01920:Or7g35	APN	9	19,496,318 (GRCm39)	missense	probably benign	0.01
R0501:Or7g35	UTSW	9	19,495,914 (GRCm39)	missense	probably damaging	1.00
R0600:Or7g35	UTSW	9	19,496,600 (GRCm39)	missense	possibly damaging	0.47
R1769:Or7g35	UTSW	9	19,496,682 (GRCm39)	missense	probably damaging	0.98
R3117:Or7g35	UTSW	9	19,496,237 (GRCm39)	missense	probably damaging	0.99
R4002:Or7g35	UTSW	9	19,496,010 (GRCm39)	missense	probably damaging	1.00
R4003:Or7g35	UTSW	9	19,496,010 (GRCm39)	missense	probably damaging	1.00
R4043:Or7g35	UTSW	9	19,496,291 (GRCm39)	missense	probably benign	0.16
R4243:Or7g35	UTSW	9	19,495,854 (GRCm39)	missense	probably damaging	1.00
R4672:Or7g35	UTSW	9	19,496,726 (GRCm39)	missense	possibly damaging	0.74
R4673:Or7g35	UTSW	9	19,496,726 (GRCm39)	missense	possibly damaging	0.74
R4959:Or7g35	UTSW	9	19,496,504 (GRCm39)	missense	probably benign
R4973:Or7g35	UTSW	9	19,496,504 (GRCm39)	missense	probably benign
R5223:Or7g35	UTSW	9	19,496,322 (GRCm39)	missense	probably benign	0.16
R5681:Or7g35	UTSW	9	19,496,195 (GRCm39)	missense	probably damaging	1.00
R6005:Or7g35	UTSW	9	19,496,181 (GRCm39)	missense	probably benign	0.45
R6017:Or7g35	UTSW	9	19,496,730 (GRCm39)	missense	probably benign	0.00
R6145:Or7g35	UTSW	9	19,496,184 (GRCm39)	missense	probably benign	0.02
R6615:Or7g35	UTSW	9	19,496,285 (GRCm39)	missense	probably benign	0.05
R6771:Or7g35	UTSW	9	19,496,675 (GRCm39)	missense	probably benign	0.16
R6969:Or7g35	UTSW	9	19,495,886 (GRCm39)	missense	possibly damaging	0.77
R7239:Or7g35	UTSW	9	19,496,487 (GRCm39)	missense	probably damaging	1.00
R7313:Or7g35	UTSW	9	19,495,938 (GRCm39)	missense	probably damaging	1.00
R7361:Or7g35	UTSW	9	19,495,856 (GRCm39)	missense	probably benign	0.00
R8112:Or7g35	UTSW	9	19,496,020 (GRCm39)	missense	probably benign	0.44
R8470:Or7g35	UTSW	9	19,496,265 (GRCm39)	missense	probably damaging	0.99
R9155:Or7g35	UTSW	9	19,496,379 (GRCm39)	missense	probably benign	0.00
R9187:Or7g35	UTSW	9	19,495,950 (GRCm39)	missense	probably benign	0.03
R9422:Or7g35	UTSW	9	19,495,968 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCATCAAGCTTTCCTGTTCTGACAC -3'
(R):5'- CACAGATGTTGACGCATAGAGAGTCC -3'

Sequencing Primer
(F):5'- GTTCTGACACCCGAATTGAC -3'
(R):5'- CGCATAGAGAGTCCAAGATTCAG -3'

Posted On

2014-08-18