Mutagenetix > Incidental Mutation

Incidental Mutation 'R1966:Muc5ac'

218960

Institutional Source

Beutler Lab

Gene Symbol

Muc5ac

Ensembl Gene

ENSMUSG00000037974

Gene Name

mucin 5, subtypes A and C, tracheobronchial/gastric

Synonyms

MGM, 2210005L13Rik

MMRRC Submission

039979-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1966 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

141342709-141372968 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 141357113 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1129 (D1129G)

Ref Sequence

ENSEMBL: ENSMUSP00000131681 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041924] [ENSMUST00000155534] [ENSMUST00000163321]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000041924
AA Change: D1129G

PolyPhen 2 Score 0.922 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000039699
Gene: ENSMUSG00000037974
AA Change: D1129G

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	1.6e-14	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	6.1e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1482	2.3e-25	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1692	2.2e-27	PFAM
Pfam:Mucin2_WxxW	1765	1857	8.6e-27	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000155534
AA Change: D1130G

PolyPhen 2 Score 0.922 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000122353
Gene: ENSMUSG00000037974
AA Change: D1130G

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	9.6e-15	PFAM
VWC	395	437	3.54e-1	SMART
VWD	422	586	2.35e-33	SMART
C8	623	697	8.42e-36	SMART
Pfam:TIL	703	760	3.6e-9	PFAM
VWC	762	826	6.75e-1	SMART
VWC	864	906	4.06e-1	SMART
VWD	891	1051	1.51e-45	SMART
C8	1087	1161	2.78e-36	SMART
low complexity region	1316	1331	N/A	INTRINSIC
low complexity region	1334	1367	N/A	INTRINSIC
low complexity region	1372	1388	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1395	1483	1.3e-25	PFAM
low complexity region	1522	1533	N/A	INTRINSIC
low complexity region	1537	1564	N/A	INTRINSIC
low complexity region	1579	1596	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1605	1693	1.3e-27	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000163321
AA Change: D1129G

PolyPhen 2 Score 0.922 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000131681
Gene: ENSMUSG00000037974
AA Change: D1129G

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	7.9e-15	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	1.9e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1481	1.1e-23	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1691	1.1e-25	PFAM
Pfam:Mucin2_WxxW	1765	1856	6.3e-24	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to T. muris infection with persistent worm burden, goblet cell hyperplasia, and increased serum IFN-gamma despite a normal TH2-type immune response. A portion of mice show corneal opacity and poor tear quality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410002F23Rik	T	A	7: 43,900,650 (GRCm39)	V185E	probably benign	Het
Abca1	A	C	4: 53,050,409 (GRCm39)	V1608G	probably damaging	Het
Ap2b1	T	C	11: 83,237,721 (GRCm39)	I557T	probably benign	Het
Arhgef15	G	T	11: 68,845,501 (GRCm39)	P117Q	probably damaging	Het
Arhgef39	T	C	4: 43,496,710 (GRCm39)	S335G	probably benign	Het
Blm	A	T	7: 80,162,934 (GRCm39)	F139Y	possibly damaging	Het
Cacna2d1	C	T	5: 16,538,783 (GRCm39)	R581*	probably null	Het
Cadps	C	A	14: 12,822,450 (GRCm38)	E97*	probably null	Het
Cavin2	T	A	1: 51,328,801 (GRCm39)	L86Q	probably damaging	Het
Ccdc136	A	G	6: 29,418,091 (GRCm39)	E787G	probably damaging	Het
Ccdc39	T	C	3: 33,880,629 (GRCm39)	K446R	probably damaging	Het
Cdh23	T	C	10: 60,159,361 (GRCm39)	Y2138C	probably damaging	Het
Cdk12	T	A	11: 98,094,916 (GRCm39)	Y241*	probably null	Het
Clcn4	T	A	7: 7,287,184 (GRCm39)	*688L	probably null	Het
Cntnap1	T	C	11: 101,071,212 (GRCm39)	V375A	possibly damaging	Het
Coq5	T	A	5: 115,432,890 (GRCm39)		probably null	Het
Cyp4b1	A	G	4: 115,483,076 (GRCm39)	I405T	probably benign	Het
Det1	T	C	7: 78,492,966 (GRCm39)	Y346C	probably damaging	Het
Enpp3	A	T	10: 24,683,389 (GRCm39)	V276E	probably damaging	Het
Epb41l2	G	A	10: 25,317,666 (GRCm39)	R61Q	probably benign	Het
Fam76b	G	A	9: 13,739,362 (GRCm39)		probably null	Het
Fbxo45	A	T	16: 32,052,048 (GRCm39)	D238E	probably benign	Het
Fnip2	G	A	3: 79,400,779 (GRCm39)	T314I	probably benign	Het
Fsip2	T	C	2: 82,823,124 (GRCm39)	S6286P	possibly damaging	Het
Gbf1	T	C	19: 46,260,003 (GRCm39)	F999L	probably damaging	Het
Gnao1	C	A	8: 94,670,827 (GRCm39)	T102K	probably benign	Het
Gpatch2	T	A	1: 187,054,498 (GRCm39)	D76E	probably damaging	Het
Gpd1l	A	G	9: 114,743,462 (GRCm39)	I146T	probably benign	Het
Grik2	A	T	10: 49,232,005 (GRCm39)	H508Q	probably damaging	Het
Hapstr1	A	G	16: 8,648,445 (GRCm39)	E41G	possibly damaging	Het
Hk3	A	T	13: 55,162,268 (GRCm39)	F112Y	probably damaging	Het
Hmcn2	T	G	2: 31,279,341 (GRCm39)	I1781S	probably damaging	Het
Inhba	A	T	13: 16,201,221 (GRCm39)	K261M	probably damaging	Het
Jarid2	T	A	13: 45,059,752 (GRCm39)	N661K	probably damaging	Het
Kcna2	T	C	3: 107,011,946 (GRCm39)	S176P	probably damaging	Het
Kcnj3	A	T	2: 55,327,343 (GRCm39)	Q44L	probably damaging	Het
Kcns1	G	T	2: 164,010,455 (GRCm39)	F101L	probably damaging	Het
Kdm5b	T	A	1: 134,541,611 (GRCm39)		probably null	Het
Klhl14	C	T	18: 21,687,730 (GRCm39)	G564D	probably damaging	Het
Klhl18	A	T	9: 110,305,658 (GRCm39)	V2E	probably benign	Het
Klhl6	T	C	16: 19,801,572 (GRCm39)	E61G	probably damaging	Het
Krt1	T	C	15: 101,757,427 (GRCm39)	D261G	probably benign	Het
Lama5	C	A	2: 179,830,145 (GRCm39)	C1896F	probably damaging	Het
Lrba	A	G	3: 86,513,175 (GRCm39)		probably null	Het
Lrch3	A	G	16: 32,734,755 (GRCm39)	T82A	possibly damaging	Het
Maml3	C	G	3: 52,011,560 (GRCm39)	G2A	unknown	Het
Mapkap1	T	A	2: 34,408,691 (GRCm39)	N34K	probably damaging	Het
Muc13	A	T	16: 33,634,909 (GRCm39)	I488F	probably damaging	Het
Nacc1	A	T	8: 85,403,010 (GRCm39)	N288K	probably damaging	Het
Nek1	T	G	8: 61,469,330 (GRCm39)	I129R	probably damaging	Het
Nf1	T	A	11: 79,302,390 (GRCm39)	S319R	possibly damaging	Het
Nle1	C	T	11: 82,792,614 (GRCm39)	G432D	probably damaging	Het
Nol4l	A	G	2: 153,371,375 (GRCm39)	V103A	probably benign	Het
Oca2	A	G	7: 56,064,215 (GRCm39)	I737V	probably damaging	Het
Or12k5	A	T	2: 36,894,960 (GRCm39)	F222Y	possibly damaging	Het
Or1j17	G	A	2: 36,578,796 (GRCm39)	V261I	probably benign	Het
Or4b1	T	C	2: 89,979,748 (GRCm39)	S201G	probably damaging	Het
Or51l4	G	T	7: 103,404,375 (GRCm39)	T139K	probably damaging	Het
Or52ac1	A	G	7: 104,246,008 (GRCm39)	C127R	probably damaging	Het
Or6k6	G	T	1: 173,944,817 (GRCm39)	S255*	probably null	Het
Or6s1	T	A	14: 51,308,614 (GRCm39)	I79F	probably damaging	Het
Orc1	T	A	4: 108,469,414 (GRCm39)	I746N	probably damaging	Het
Pbxip1	A	G	3: 89,352,795 (GRCm39)	D147G	probably damaging	Het
Pirb	A	T	7: 3,720,637 (GRCm39)	L287Q	probably benign	Het
Plin5	T	A	17: 56,419,186 (GRCm39)	D412V	probably damaging	Het
Plxna2	A	T	1: 194,327,008 (GRCm39)	Y314F	possibly damaging	Het
Ppid	A	T	3: 79,509,606 (GRCm39)	K308*	probably null	Het
Prss38	T	C	11: 59,264,310 (GRCm39)	Y219C	probably damaging	Het
Ptx3	C	T	3: 66,132,042 (GRCm39)	R188C	probably damaging	Het
Ralgds	T	A	2: 28,435,887 (GRCm39)	V504E	probably damaging	Het
Rere	T	C	4: 150,701,330 (GRCm39)	Y1237H	probably damaging	Het
Rpp30	C	T	19: 36,066,549 (GRCm39)	S94L	probably damaging	Het
Rrp15	C	T	1: 186,468,402 (GRCm39)	V205I	possibly damaging	Het
Scpep1	A	G	11: 88,843,240 (GRCm39)	W73R	probably damaging	Het
Sec23ip	A	G	7: 128,357,077 (GRCm39)	H376R	probably damaging	Het
Serping1	G	T	2: 84,596,072 (GRCm39)	T454K	probably damaging	Het
Shtn1	T	G	19: 58,963,470 (GRCm39)	Y615S	probably benign	Het
Slc20a2	C	A	8: 23,035,553 (GRCm39)	P184T	probably damaging	Het
Slc22a29	C	A	19: 8,195,772 (GRCm39)	R89L	probably damaging	Het
Slc2a2	A	G	3: 28,773,634 (GRCm39)	Q313R	probably damaging	Het
Tas2r122	A	T	6: 132,688,157 (GRCm39)	Y245*	probably null	Het
Ticrr	C	A	7: 79,344,483 (GRCm39)	C1449*	probably null	Het
Tmem181a	T	C	17: 6,353,501 (GRCm39)	V412A	probably benign	Het
Tmtc4	T	A	14: 123,165,011 (GRCm39)	E616V	probably benign	Het
Tnrc6b	A	G	15: 80,764,640 (GRCm39)	K714R	probably damaging	Het
Trpm8	T	A	1: 88,260,470 (GRCm39)		probably null	Het
Ubr2	T	C	17: 47,265,845 (GRCm39)	T1163A	probably benign	Het
Ubr4	A	G	4: 139,178,555 (GRCm39)		probably null	Het
Ulk2	A	T	11: 61,710,297 (GRCm39)		probably null	Het
Ulk4	T	A	9: 121,086,182 (GRCm39)	M350L	probably benign	Het
Vmn1r185	T	C	7: 26,310,956 (GRCm39)	E183G	probably benign	Het
Vmn1r76	T	A	7: 11,664,441 (GRCm39)	I223F	probably damaging	Het
Wdr27	T	C	17: 15,154,861 (GRCm39)	T19A	possibly damaging	Het
Wdr59	T	G	8: 112,177,535 (GRCm39)	T973P	possibly damaging	Het
Wnk2	A	G	13: 49,192,487 (GRCm39)	S664P	probably damaging	Het
Zfp1004	T	A	2: 150,033,827 (GRCm39)	D49E	probably benign	Het
Zfp120	A	T	2: 149,959,318 (GRCm39)	C335S	probably damaging	Het
Zfp30	C	A	7: 29,491,877 (GRCm39)	Q44K	probably benign	Het
Zfp541	T	C	7: 15,812,996 (GRCm39)	S550P	probably benign	Het
Zw10	C	A	9: 48,980,133 (GRCm39)	N421K	probably damaging	Het

Other mutations in Muc5ac

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Muc5ac	APN	7	141,366,440 (GRCm39)	missense	possibly damaging	0.93
IGL01064:Muc5ac	APN	7	141,361,210 (GRCm39)	missense	probably benign	0.12
IGL01155:Muc5ac	APN	7	141,360,680 (GRCm39)	splice site	probably benign
IGL01452:Muc5ac	APN	7	141,371,292 (GRCm39)	missense	probably benign	0.00
IGL01590:Muc5ac	APN	7	141,352,630 (GRCm39)	missense	probably benign	0.02
IGL02104:Muc5ac	APN	7	141,364,815 (GRCm39)	missense	probably damaging	0.98
IGL02152:Muc5ac	APN	7	141,353,914 (GRCm39)	missense	possibly damaging	0.86
IGL02153:Muc5ac	APN	7	141,372,537 (GRCm39)	nonsense	probably null
IGL02178:Muc5ac	APN	7	141,359,184 (GRCm39)	splice site	probably benign
IGL02403:Muc5ac	APN	7	141,357,187 (GRCm39)	missense	possibly damaging	0.71
IGL02576:Muc5ac	APN	7	141,370,781 (GRCm39)	missense	probably benign	0.01
IGL02665:Muc5ac	APN	7	141,344,823 (GRCm39)	missense	possibly damaging	0.71
IGL02704:Muc5ac	APN	7	141,349,000 (GRCm39)	missense	possibly damaging	0.71
IGL02808:Muc5ac	APN	7	141,359,512 (GRCm39)	missense	possibly damaging	0.72
IGL03283:Muc5ac	APN	7	141,367,518 (GRCm39)	missense	probably benign	0.34
IGL03384:Muc5ac	APN	7	141,366,140 (GRCm39)	missense	possibly damaging	0.71
IGL03046:Muc5ac	UTSW	7	141,348,950 (GRCm39)	missense	probably benign	0.27
PIT4515001:Muc5ac	UTSW	7	141,361,153 (GRCm39)	missense	probably damaging	0.99
R0092:Muc5ac	UTSW	7	141,372,367 (GRCm39)	missense	possibly damaging	0.72
R0145:Muc5ac	UTSW	7	141,349,012 (GRCm39)	missense	possibly damaging	0.71
R0147:Muc5ac	UTSW	7	141,364,776 (GRCm39)	missense	probably benign	0.08
R0363:Muc5ac	UTSW	7	141,354,697 (GRCm39)	missense	probably benign	0.01
R0384:Muc5ac	UTSW	7	141,365,988 (GRCm39)	missense	possibly damaging	0.71
R0440:Muc5ac	UTSW	7	141,345,771 (GRCm39)	nonsense	probably null
R0583:Muc5ac	UTSW	7	141,361,345 (GRCm39)	missense	probably damaging	0.99
R0616:Muc5ac	UTSW	7	141,349,981 (GRCm39)	missense	probably benign	0.02
R0682:Muc5ac	UTSW	7	141,359,406 (GRCm39)	missense	possibly damaging	0.53
R0685:Muc5ac	UTSW	7	141,361,446 (GRCm39)	missense	probably benign	0.03
R0883:Muc5ac	UTSW	7	141,350,002 (GRCm39)	missense	possibly damaging	0.71
R0924:Muc5ac	UTSW	7	141,361,252 (GRCm39)	missense	possibly damaging	0.68
R1300:Muc5ac	UTSW	7	141,370,666 (GRCm39)	missense	possibly damaging	0.73
R1315:Muc5ac	UTSW	7	141,361,060 (GRCm39)	missense	probably damaging	0.99
R1354:Muc5ac	UTSW	7	141,361,114 (GRCm39)	missense	probably damaging	0.99
R1484:Muc5ac	UTSW	7	141,367,629 (GRCm39)	splice site	probably null
R1599:Muc5ac	UTSW	7	141,352,640 (GRCm39)	missense	possibly damaging	0.52
R1758:Muc5ac	UTSW	7	141,355,268 (GRCm39)	missense	possibly damaging	0.86
R1837:Muc5ac	UTSW	7	141,360,823 (GRCm39)	missense	probably benign	0.00
R1911:Muc5ac	UTSW	7	141,350,041 (GRCm39)	missense	probably benign	0.18
R1922:Muc5ac	UTSW	7	141,347,426 (GRCm39)	missense	probably benign	0.03
R1994:Muc5ac	UTSW	7	141,366,889 (GRCm39)	missense	possibly damaging	0.93
R2056:Muc5ac	UTSW	7	141,345,772 (GRCm39)	missense	probably benign	0.01
R2126:Muc5ac	UTSW	7	141,364,479 (GRCm39)	missense	possibly damaging	0.84
R2170:Muc5ac	UTSW	7	141,366,084 (GRCm39)	missense	possibly damaging	0.93
R2258:Muc5ac	UTSW	7	141,344,745 (GRCm39)	missense	probably benign	0.41
R2259:Muc5ac	UTSW	7	141,344,745 (GRCm39)	missense	probably benign	0.41
R2293:Muc5ac	UTSW	7	141,360,936 (GRCm39)	missense	probably damaging	0.99
R2435:Muc5ac	UTSW	7	141,371,841 (GRCm39)	missense	possibly damaging	0.53
R2895:Muc5ac	UTSW	7	141,344,877 (GRCm39)	missense	possibly damaging	0.92
R2910:Muc5ac	UTSW	7	141,361,378 (GRCm39)	missense	probably damaging	0.99
R3154:Muc5ac	UTSW	7	141,346,473 (GRCm39)	splice site	probably null
R3762:Muc5ac	UTSW	7	141,361,212 (GRCm39)	missense	possibly damaging	0.53
R3791:Muc5ac	UTSW	7	141,352,238 (GRCm39)	missense	probably benign	0.32
R3806:Muc5ac	UTSW	7	141,367,471 (GRCm39)	missense	possibly damaging	0.91
R3825:Muc5ac	UTSW	7	141,368,460 (GRCm39)	missense	possibly damaging	0.92
R3888:Muc5ac	UTSW	7	141,344,961 (GRCm39)	missense	possibly damaging	0.51
R3929:Muc5ac	UTSW	7	141,356,629 (GRCm39)	missense	probably benign
R3981:Muc5ac	UTSW	7	141,367,512 (GRCm39)	missense	possibly damaging	0.86
R4034:Muc5ac	UTSW	7	141,353,581 (GRCm39)	critical splice donor site	probably null
R4043:Muc5ac	UTSW	7	141,361,215 (GRCm39)	missense	possibly damaging	0.53
R4061:Muc5ac	UTSW	7	141,364,867 (GRCm39)	missense	possibly damaging	0.85
R4106:Muc5ac	UTSW	7	141,356,572 (GRCm39)	missense	possibly damaging	0.86
R4206:Muc5ac	UTSW	7	141,370,847 (GRCm39)	missense	possibly damaging	0.73
R4613:Muc5ac	UTSW	7	141,344,840 (GRCm39)	missense	possibly damaging	0.93
R4719:Muc5ac	UTSW	7	141,343,500 (GRCm39)	missense	possibly damaging	0.83
R4751:Muc5ac	UTSW	7	141,371,338 (GRCm39)	missense	probably benign	0.00
R4789:Muc5ac	UTSW	7	141,352,619 (GRCm39)	missense	possibly damaging	0.86
R4928:Muc5ac	UTSW	7	141,371,639 (GRCm39)	nonsense	probably null
R4971:Muc5ac	UTSW	7	141,370,015 (GRCm39)	missense	possibly damaging	0.68
R4982:Muc5ac	UTSW	7	141,363,193 (GRCm39)	intron	probably benign
R5088:Muc5ac	UTSW	7	141,350,056 (GRCm39)	missense	possibly damaging	0.53
R5141:Muc5ac	UTSW	7	141,368,479 (GRCm39)	missense	possibly damaging	0.72
R5224:Muc5ac	UTSW	7	141,347,708 (GRCm39)	missense	probably benign	0.32
R5366:Muc5ac	UTSW	7	141,361,287 (GRCm39)	missense	probably benign	0.01
R5497:Muc5ac	UTSW	7	141,361,380 (GRCm39)	missense	probably damaging	0.99
R5507:Muc5ac	UTSW	7	141,361,569 (GRCm39)	missense	possibly damaging	0.72
R5643:Muc5ac	UTSW	7	141,347,452 (GRCm39)	critical splice donor site	probably null
R5811:Muc5ac	UTSW	7	141,352,721 (GRCm39)	missense	possibly damaging	0.51
R5946:Muc5ac	UTSW	7	141,371,644 (GRCm39)	missense	possibly damaging	0.73
R5970:Muc5ac	UTSW	7	141,344,406 (GRCm39)	nonsense	probably null
R5977:Muc5ac	UTSW	7	141,350,104 (GRCm39)	missense	possibly damaging	0.73
R6051:Muc5ac	UTSW	7	141,365,594 (GRCm39)	missense	possibly damaging	0.53
R6126:Muc5ac	UTSW	7	141,354,969 (GRCm39)	missense	possibly damaging	0.71
R6159:Muc5ac	UTSW	7	141,369,323 (GRCm39)	missense	possibly damaging	0.53
R6256:Muc5ac	UTSW	7	141,343,532 (GRCm39)	missense	possibly damaging	0.53
R6283:Muc5ac	UTSW	7	141,370,601 (GRCm39)	nonsense	probably null
R6341:Muc5ac	UTSW	7	141,355,229 (GRCm39)	missense	probably damaging	0.99
R6356:Muc5ac	UTSW	7	141,366,416 (GRCm39)	missense	probably benign	0.05
R6481:Muc5ac	UTSW	7	141,362,808 (GRCm39)	intron	probably benign
R6483:Muc5ac	UTSW	7	141,356,591 (GRCm39)	missense	probably benign	0.18
R6627:Muc5ac	UTSW	7	141,362,427 (GRCm39)	intron	probably benign
R6636:Muc5ac	UTSW	7	141,372,342 (GRCm39)	missense	possibly damaging	0.86
R6637:Muc5ac	UTSW	7	141,372,342 (GRCm39)	missense	possibly damaging	0.86
R6656:Muc5ac	UTSW	7	141,357,065 (GRCm39)	missense	probably damaging	0.98
R6721:Muc5ac	UTSW	7	141,352,729 (GRCm39)	missense	possibly damaging	0.71
R6794:Muc5ac	UTSW	7	141,363,289 (GRCm39)	intron	probably benign
R6844:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6847:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6852:Muc5ac	UTSW	7	141,370,644 (GRCm39)	missense	probably benign	0.03
R6862:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6863:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6864:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6865:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6874:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6875:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6876:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6877:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6889:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6920:Muc5ac	UTSW	7	141,347,035 (GRCm39)	missense	possibly damaging	0.86
R6998:Muc5ac	UTSW	7	141,372,451 (GRCm39)	missense	possibly damaging	0.92
R7017:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7091:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,363,385 (GRCm39)	intron	probably benign
R7110:Muc5ac	UTSW	7	141,353,559 (GRCm39)	missense	possibly damaging	0.95
R7117:Muc5ac	UTSW	7	141,367,559 (GRCm39)	nonsense	probably null
R7238:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7238:Muc5ac	UTSW	7	141,363,254 (GRCm39)	missense	unknown
R7396:Muc5ac	UTSW	7	141,362,152 (GRCm39)	missense	unknown
R7456:Muc5ac	UTSW	7	141,346,904 (GRCm39)	missense	probably benign	0.32
R7477:Muc5ac	UTSW	7	141,370,019 (GRCm39)	missense	possibly damaging	0.72
R7530:Muc5ac	UTSW	7	141,367,536 (GRCm39)	missense	possibly damaging	0.51
R7545:Muc5ac	UTSW	7	141,362,405 (GRCm39)	missense	unknown
R7604:Muc5ac	UTSW	7	141,363,446 (GRCm39)	missense	unknown
R7635:Muc5ac	UTSW	7	141,359,413 (GRCm39)	missense	probably damaging	0.98
R7635:Muc5ac	UTSW	7	141,359,490 (GRCm39)	missense	possibly damaging	0.53
R7650:Muc5ac	UTSW	7	141,363,159 (GRCm39)	missense	unknown
R7651:Muc5ac	UTSW	7	141,349,991 (GRCm39)	missense	possibly damaging	0.92
R7685:Muc5ac	UTSW	7	141,363,120 (GRCm39)	missense	unknown
R7720:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7749:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7750:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7751:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7754:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7798:Muc5ac	UTSW	7	141,347,778 (GRCm39)	critical splice donor site	probably null
R7835:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7837:Muc5ac	UTSW	7	141,369,700 (GRCm39)	missense	possibly damaging	0.53
R7858:Muc5ac	UTSW	7	141,357,166 (GRCm39)	missense	possibly damaging	0.51
R7866:Muc5ac	UTSW	7	141,349,589 (GRCm39)	missense	probably benign	0.00
R7874:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7876:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7877:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7881:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7884:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7921:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7976:Muc5ac	UTSW	7	141,363,528 (GRCm39)	missense	unknown
R8104:Muc5ac	UTSW	7	141,358,520 (GRCm39)	missense	possibly damaging	0.96
R8177:Muc5ac	UTSW	7	141,361,068 (GRCm39)	missense	probably damaging	1.00
R8214:Muc5ac	UTSW	7	141,356,685 (GRCm39)	missense	possibly damaging	0.53
R8292:Muc5ac	UTSW	7	141,363,000 (GRCm39)	missense	unknown
R8386:Muc5ac	UTSW	7	141,361,371 (GRCm39)	missense	possibly damaging	0.93
R8400:Muc5ac	UTSW	7	141,364,213 (GRCm39)	missense	probably damaging	0.99
R8504:Muc5ac	UTSW	7	141,360,892 (GRCm39)	missense	probably damaging	1.00
R8709:Muc5ac	UTSW	7	141,370,663 (GRCm39)	missense	possibly damaging	0.96
R8725:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R8727:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R8754:Muc5ac	UTSW	7	141,354,008 (GRCm39)	missense	possibly damaging	0.85
R8769:Muc5ac	UTSW	7	141,372,609 (GRCm39)	missense	probably damaging	1.00
R8933:Muc5ac	UTSW	7	141,343,493 (GRCm39)	missense	possibly damaging	0.59
R8939:Muc5ac	UTSW	7	141,347,091 (GRCm39)	missense	probably damaging	0.98
R9049:Muc5ac	UTSW	7	141,362,712 (GRCm39)	missense	unknown
R9124:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9131:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9132:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9135:Muc5ac	UTSW	7	141,352,218 (GRCm39)	missense	probably damaging	0.99
R9156:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9157:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9159:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9160:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9161:Muc5ac	UTSW	7	141,353,026 (GRCm39)	missense	possibly damaging	0.53
R9175:Muc5ac	UTSW	7	141,366,093 (GRCm39)	missense	possibly damaging	0.92
R9183:Muc5ac	UTSW	7	141,352,637 (GRCm39)	missense	possibly damaging	0.71
R9218:Muc5ac	UTSW	7	141,361,098 (GRCm39)	missense	probably damaging	0.99
R9219:Muc5ac	UTSW	7	141,370,800 (GRCm39)	nonsense	probably null
R9239:Muc5ac	UTSW	7	141,353,954 (GRCm39)	missense	probably damaging	0.99
R9246:Muc5ac	UTSW	7	141,364,215 (GRCm39)	missense	probably benign	0.11
R9287:Muc5ac	UTSW	7	141,361,626 (GRCm39)	missense	probably damaging	0.99
R9320:Muc5ac	UTSW	7	141,369,255 (GRCm39)	missense	probably benign	0.01
R9327:Muc5ac	UTSW	7	141,365,429 (GRCm39)	missense	possibly damaging	0.86
R9428:Muc5ac	UTSW	7	141,362,559 (GRCm39)	missense	unknown
R9430:Muc5ac	UTSW	7	141,362,569 (GRCm39)	missense	unknown
R9454:Muc5ac	UTSW	7	141,362,431 (GRCm39)	missense	unknown
R9483:Muc5ac	UTSW	7	141,365,465 (GRCm39)	nonsense	probably null
R9581:Muc5ac	UTSW	7	141,363,799 (GRCm39)	missense	unknown
R9610:Muc5ac	UTSW	7	141,350,078 (GRCm39)	missense	possibly damaging	0.86
R9642:Muc5ac	UTSW	7	141,349,601 (GRCm39)	missense	possibly damaging	0.71
R9684:Muc5ac	UTSW	7	141,364,798 (GRCm39)	missense	probably benign	0.41
R9760:Muc5ac	UTSW	7	141,360,985 (GRCm39)	missense	probably benign	0.05
R9778:Muc5ac	UTSW	7	141,349,021 (GRCm39)	nonsense	probably null
X0060:Muc5ac	UTSW	7	141,357,070 (GRCm39)	missense	possibly damaging	0.71
Z1088:Muc5ac	UTSW	7	141,365,429 (GRCm39)	missense	possibly damaging	0.86
Z1088:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
Z1177:Muc5ac	UTSW	7	141,371,777 (GRCm39)	missense	probably benign	0.33
Z1177:Muc5ac	UTSW	7	141,362,961 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACATGGACCGAGGAAGCTTC -3'
(R):5'- ACTGGCCAGAACCAAGTCAG -3'

Sequencing Primer
(F):5'- TGGCCGGATATTCACAGTCATAC -3'
(R):5'- CCAGAACCAAGTCAGAAGGAGC -3'

Posted On

2014-08-25