Mutagenetix > Incidental Mutation

Incidental Mutation 'R1967:Vmn2r99'

219116

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

039980-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R1967 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19378815 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 254 (T254S)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably benign
Transcript: ENSMUST00000176107
AA Change: T254S

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: T254S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989
AA Change: T254S

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	T	G	15: 8,203,420 (GRCm38)	V1141G	probably benign	Het
4930447A16Rik	A	C	15: 37,439,598 (GRCm38)		probably benign	Het
4931408C20Rik	T	A	1: 26,683,373 (GRCm38)	I909L	probably benign	Het
Abca6	T	C	11: 110,187,148 (GRCm38)	I1278V	probably benign	Het
Adamts15	C	T	9: 30,921,309 (GRCm38)	W310*	probably null	Het
Adamts6	T	A	13: 104,426,951 (GRCm38)	C650*	probably null	Het
Akap3	G	T	6: 126,865,098 (GRCm38)	G227C	probably benign	Het
Alox12e	A	T	11: 70,317,856 (GRCm38)	S457T	probably benign	Het
Angpt1	C	A	15: 42,438,307 (GRCm38)	C435F	probably damaging	Het
Anxa9	T	C	3: 95,300,608 (GRCm38)	Q207R	probably benign	Het
Aplnr	A	T	2: 85,137,606 (GRCm38)	D325V	probably benign	Het
Art2b	A	C	7: 101,580,207 (GRCm38)	F162V	probably damaging	Het
Atp13a4	A	G	16: 29,479,854 (GRCm38)	S96P	probably damaging	Het
Bnc1	A	T	7: 81,973,636 (GRCm38)	H614Q	probably benign	Het
C6	A	G	15: 4,759,820 (GRCm38)	D249G	probably damaging	Het
Camta1	A	T	4: 151,088,973 (GRCm38)	F977I	probably damaging	Het
Chil6	T	C	3: 106,391,154 (GRCm38)	S188G	possibly damaging	Het
Cntrob	A	T	11: 69,320,963 (GRCm38)	L145Q	probably damaging	Het
Coro7	T	C	16: 4,634,889 (GRCm38)	E306G	probably damaging	Het
Cpq	T	C	15: 33,497,202 (GRCm38)	S363P	possibly damaging	Het
Ctc1	A	G	11: 69,027,862 (GRCm38)		probably null	Het
Defb43	T	A	14: 63,017,797 (GRCm38)	N26K	probably benign	Het
Dennd1a	T	C	2: 37,844,833 (GRCm38)	T41A	probably benign	Het
Dnah11	A	C	12: 117,916,788 (GRCm38)	Y3866D	probably damaging	Het
Eml6	A	G	11: 30,024,545 (GRCm38)	L11P	probably damaging	Het
Epha5	A	G	5: 84,416,429 (GRCm38)	V26A	probably benign	Het
Fam13c	A	G	10: 70,551,735 (GRCm38)	D443G	probably damaging	Het
Fam227a	G	A	15: 79,637,134 (GRCm38)	L243F	possibly damaging	Het
Fat3	T	G	9: 15,968,295 (GRCm38)	R3301S	probably benign	Het
Fbxw20	T	C	9: 109,217,510 (GRCm38)	T461A	probably benign	Het
Fgf8	T	A	19: 45,741,568 (GRCm38)	S61C	probably damaging	Het
Fgfrl1	A	G	5: 108,705,005 (GRCm38)	E100G	probably damaging	Het
Gabra4	G	A	5: 71,572,069 (GRCm38)	S456F	possibly damaging	Het
Gas6	T	C	8: 13,470,317 (GRCm38)	E457G	probably damaging	Het
Gm156	A	T	6: 129,775,835 (GRCm38)	N2K	possibly damaging	Het
Gpr33	G	A	12: 52,024,208 (GRCm38)	S16L	probably benign	Het
Greb1l	A	C	18: 10,501,049 (GRCm38)	N393T	possibly damaging	Het
Gtpbp4	T	C	13: 8,977,304 (GRCm38)	K492E	probably benign	Het
Hsf2bp	A	T	17: 31,987,404 (GRCm38)	L251*	probably null	Het
Hyal5	A	T	6: 24,876,194 (GRCm38)	Q22L	possibly damaging	Het
Itih1	A	T	14: 30,941,984 (GRCm38)	V114E	possibly damaging	Het
Jak3	T	A	8: 71,681,535 (GRCm38)	I427N	probably damaging	Het
Jcad	T	C	18: 4,675,162 (GRCm38)	S975P	probably benign	Het
Kcng4	A	G	8: 119,632,923 (GRCm38)	V238A	probably damaging	Het
Klb	A	G	5: 65,372,074 (GRCm38)	D315G	probably damaging	Het
Krt5	T	C	15: 101,711,659 (GRCm38)	N208D	probably benign	Het
Lmo7	T	A	14: 101,900,215 (GRCm38)	H551Q	probably benign	Het
Lrp8	G	T	4: 107,859,971 (GRCm38)	G732V	probably damaging	Het
Lrrc38	A	G	4: 143,369,983 (GRCm38)	D288G	unknown	Het
Lrrc4b	GAGAAG	GAG	7: 44,462,230 (GRCm38)		probably benign	Het
Mas1	A	G	17: 12,842,036 (GRCm38)	Y167H	probably benign	Het
Mcm8	A	C	2: 132,842,742 (GRCm38)	I759L	probably benign	Het
Med13l	G	A	5: 118,761,322 (GRCm38)	D2148N	probably damaging	Het
Muc20	A	T	16: 32,794,242 (GRCm38)	I255K	probably benign	Het
Myh1	A	G	11: 67,213,447 (GRCm38)	N980S	probably benign	Het
Nap1l4	T	A	7: 143,534,287 (GRCm38)	Q178L	probably damaging	Het
Ncapg	T	A	5: 45,699,910 (GRCm38)	L988Q	probably damaging	Het
Ncf2	A	T	1: 152,830,372 (GRCm38)	H245L	probably damaging	Het
Neurl4	A	G	11: 69,903,210 (GRCm38)	E164G	possibly damaging	Het
Nf1	G	A	11: 79,412,745 (GRCm38)	R416H	probably damaging	Het
Obscn	G	A	11: 59,135,709 (GRCm38)	Q223*	probably null	Het
Olfr1394	T	C	11: 49,160,848 (GRCm38)	I278T	probably benign	Het
Olfr1494	T	A	19: 13,750,053 (GRCm38)	*316R	probably null	Het
Pcdhb16	T	A	18: 37,479,662 (GRCm38)	N558K	probably damaging	Het
Pcnx2	A	T	8: 125,815,683 (GRCm38)	M1253K	possibly damaging	Het
Pla2g4a	A	G	1: 149,922,081 (GRCm38)	V22A	probably damaging	Het
Plppr5	G	A	3: 117,625,906 (GRCm38)		probably null	Het
Pnpla2	T	C	7: 141,459,432 (GRCm38)	S353P	probably benign	Het
Pom121	A	T	5: 135,391,754 (GRCm38)	L271Q	unknown	Het
Prepl	A	T	17: 85,088,551 (GRCm38)	M1K	probably null	Het
Prr14l	A	G	5: 32,844,469 (GRCm38)		probably benign	Het
Psmc3	C	A	2: 91,057,844 (GRCm38)	P325T	probably benign	Het
Psme2b	A	G	11: 48,946,069 (GRCm38)	V17A	probably damaging	Het
Ptpro	A	G	6: 137,416,865 (GRCm38)	I23V	probably benign	Het
Rab31	A	G	17: 65,772,504 (GRCm38)		probably null	Het
Ranbp6	T	A	19: 29,812,500 (GRCm38)	K151*	probably null	Het
Rbm12b1	A	T	4: 12,146,304 (GRCm38)	I759L	probably benign	Het
Rgs5	T	C	1: 169,676,856 (GRCm38)	I25T	probably benign	Het
Rnf213	T	C	11: 119,480,895 (GRCm38)	V4842A	probably damaging	Het
Rtl9	A	T	X: 143,103,041 (GRCm38)	I1150F	probably damaging	Het
Scn1a	A	T	2: 66,328,425 (GRCm38)	W384R	probably damaging	Het
Sema5a	C	T	15: 32,681,619 (GRCm38)	P948L	probably damaging	Het
Slc26a3	G	A	12: 31,465,778 (GRCm38)	R559Q	probably damaging	Het
Slc29a3	A	G	10: 60,716,464 (GRCm38)	V267A	probably benign	Het
Spata22	A	G	11: 73,331,127 (GRCm38)		probably benign	Het
Sptbn2	G	T	19: 4,745,299 (GRCm38)	R1595L	probably benign	Het
Tfdp1	T	C	8: 13,373,039 (GRCm38)	S315P	possibly damaging	Het
Tle1	A	T	4: 72,120,226 (GRCm38)	V688E	probably damaging	Het
Tmem129	A	T	5: 33,655,321 (GRCm38)		probably null	Het
Tmprss11a	G	A	5: 86,431,843 (GRCm38)	T91I	probably benign	Het
Tnfsf14	A	G	17: 57,190,807 (GRCm38)	Y142H	probably damaging	Het
Tph1	T	C	7: 46,662,114 (GRCm38)	D68G	probably benign	Het
Ttn	A	T	2: 76,762,287 (GRCm38)	S12507T	probably damaging	Het
Tubgcp2	T	A	7: 140,006,153 (GRCm38)	M408L	probably benign	Het
Usp34	A	T	11: 23,364,503 (GRCm38)	H815L	probably benign	Het
Usp6nl	G	A	2: 6,441,519 (GRCm38)	R746H	probably benign	Het
Utp20	A	G	10: 88,816,979 (GRCm38)	S358P	probably benign	Het
Vmn2r118	G	A	17: 55,592,882 (GRCm38)	T674I	probably damaging	Het
Vmn2r8	A	T	5: 108,802,383 (GRCm38)	H199Q	probably benign	Het
Vmn2r9	A	G	5: 108,847,522 (GRCm38)	V420A	probably benign	Het
Zbed5	A	G	5: 129,901,669 (GRCm38)	H132R	possibly damaging	Het
Zfp568	A	G	7: 29,989,088 (GRCm38)	E25G	probably damaging	Het
Zfp951	T	A	5: 104,817,000 (GRCm38)	I67L	possibly damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,394,373 (GRCm38)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,362,135 (GRCm38)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,378,110 (GRCm38)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,394,564 (GRCm38)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,393,710 (GRCm38)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- GACCGTGGTCAGTATCCTTC -3'
(R):5'- CAAATGCCACAGCTTAGGAAG -3'

Sequencing Primer
(F):5'- GATGGCCCCCAAGGATATCTCTC -3'
(R):5'- TGCCACAGCTTAGGAAGATAAATGTC -3'

Posted On

2014-08-25