Mutagenetix > Incidental Mutation

Incidental Mutation 'R0138:Nkx6-3'

22109

Institutional Source

Beutler Lab

Gene Symbol

Nkx6-3

Ensembl Gene

ENSMUSG00000063672

Gene Name

NK6 homeobox 3

Synonyms

9130417I07Rik, Nkx6.3

MMRRC Submission

038423-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.162) question?

Stock #

R0138 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

23643287-23648964 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23643607 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 3 (S3P)

Ref Sequence

ENSEMBL: ENSMUSP00000071517 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033947] [ENSMUST00000071588] [ENSMUST00000084038] [ENSMUST00000110688] [ENSMUST00000117296] [ENSMUST00000117662] [ENSMUST00000118733] [ENSMUST00000173248] [ENSMUST00000121802] [ENSMUST00000123418] [ENSMUST00000173573] [ENSMUST00000141784] [ENSMUST00000121075]

AlphaFold

Q3UHX8

Predicted Effect

probably benign
Transcript: ENSMUST00000033947

SMART Domains

Protein: ENSMUSP00000033947
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000071588
AA Change: S3P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000071517
Gene: ENSMUSG00000063672
AA Change: S3P

Domain	Start	End	E-Value	Type
HOX	140	202	3.88e-24	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000084038

SMART Domains

Protein: ENSMUSP00000081051
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	15	44	2.5e3	SMART
ANK	48	77	3.26e0	SMART
ANK	81	110	3.15e-7	SMART
ANK	114	143	9.05e-8	SMART
ANK	147	175	4.67e-1	SMART
ANK	176	205	1.42e0	SMART
ANK	209	238	4.39e-6	SMART
ANK	242	271	1.33e-5	SMART
ANK	275	304	7.53e-5	SMART
ANK	308	337	2.35e-6	SMART
ANK	341	370	6.65e-6	SMART
ANK	374	403	5.2e-8	SMART
ANK	407	436	8.78e-6	SMART
ANK	440	469	7.53e-5	SMART
ANK	473	502	5.49e-7	SMART
ANK	506	535	2.58e-3	SMART
ANK	539	568	1.88e-5	SMART
ANK	572	601	1.02e-6	SMART
ANK	605	634	7.64e-6	SMART
ANK	638	669	3.23e-4	SMART
ANK	671	700	1.38e-3	SMART
ANK	704	733	1.58e-7	SMART
ANK	737	766	2.85e-5	SMART
ZU5	923	1027	1.9e-60	SMART
low complexity region	1050	1059	N/A	INTRINSIC
low complexity region	1387	1397	N/A	INTRINSIC
DEATH	1405	1499	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000110688

SMART Domains

Protein: ENSMUSP00000106316
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
coiled coil region	1	39	N/A	INTRINSIC
ANK	44	73	2.5e3	SMART
ANK	77	106	3.26e0	SMART
ANK	110	139	3.15e-7	SMART
ANK	143	172	9.05e-8	SMART
ANK	176	204	4.67e-1	SMART
ANK	205	234	1.42e0	SMART
ANK	238	267	4.39e-6	SMART
ANK	271	300	1.33e-5	SMART
ANK	304	333	7.53e-5	SMART
ANK	337	366	2.35e-6	SMART
ANK	370	399	6.65e-6	SMART
ANK	403	432	5.2e-8	SMART
ANK	436	465	8.78e-6	SMART
ANK	469	498	7.53e-5	SMART
ANK	502	531	5.49e-7	SMART
ANK	535	564	2.58e-3	SMART
ANK	568	597	1.88e-5	SMART
ANK	601	630	1.02e-6	SMART
ANK	634	663	7.64e-6	SMART
ANK	667	698	3.23e-4	SMART
ANK	700	729	1.38e-3	SMART
ANK	733	762	1.58e-7	SMART
ANK	766	795	2.85e-5	SMART
ZU5	944	1048	1.9e-60	SMART
low complexity region	1071	1080	N/A	INTRINSIC
low complexity region	1408	1418	N/A	INTRINSIC
DEATH	1426	1520	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000117296

SMART Domains

Protein: ENSMUSP00000113656
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	7	36	2.5e3	SMART
ANK	40	69	3.26e0	SMART
ANK	73	102	3.15e-7	SMART
ANK	106	135	9.05e-8	SMART
ANK	139	167	4.67e-1	SMART
ANK	168	197	1.42e0	SMART
ANK	201	230	4.39e-6	SMART
ANK	234	263	1.33e-5	SMART
ANK	267	296	7.53e-5	SMART
ANK	300	329	2.35e-6	SMART
ANK	333	362	6.65e-6	SMART
ANK	366	395	5.2e-8	SMART
ANK	399	428	8.78e-6	SMART
ANK	432	461	7.53e-5	SMART
ANK	465	494	5.49e-7	SMART
ANK	498	527	2.58e-3	SMART
ANK	531	560	1.88e-5	SMART
ANK	564	593	1.02e-6	SMART
ANK	597	626	7.64e-6	SMART
ANK	630	661	3.23e-4	SMART
ANK	663	692	1.38e-3	SMART
ANK	696	725	1.58e-7	SMART
ANK	729	758	2.85e-5	SMART
ZU5	907	1011	1.9e-60	SMART
low complexity region	1034	1043	N/A	INTRINSIC
low complexity region	1371	1381	N/A	INTRINSIC
DEATH	1389	1483	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000117662

SMART Domains

Protein: ENSMUSP00000113531
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	7	36	2.5e3	SMART
ANK	40	69	3.26e0	SMART
ANK	73	102	3.15e-7	SMART
ANK	106	135	9.05e-8	SMART
ANK	139	167	4.67e-1	SMART
ANK	168	197	1.42e0	SMART
ANK	201	230	4.39e-6	SMART
ANK	234	263	1.33e-5	SMART
ANK	267	296	7.53e-5	SMART
ANK	300	329	2.35e-6	SMART
ANK	333	362	6.65e-6	SMART
ANK	366	395	5.2e-8	SMART
ANK	399	428	8.78e-6	SMART
ANK	432	461	7.53e-5	SMART
ANK	465	494	5.49e-7	SMART
ANK	498	527	2.58e-3	SMART
ANK	531	560	1.88e-5	SMART
ANK	564	593	1.02e-6	SMART
ANK	597	626	7.64e-6	SMART
ANK	630	661	3.23e-4	SMART
ANK	663	692	1.38e-3	SMART
ANK	696	725	1.58e-7	SMART
ANK	729	758	2.85e-5	SMART
ZU5	907	1011	1.9e-60	SMART
low complexity region	1034	1043	N/A	INTRINSIC
low complexity region	1371	1381	N/A	INTRINSIC
DEATH	1389	1483	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000118733

SMART Domains

Protein: ENSMUSP00000112850
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	15	44	2.5e3	SMART
ANK	48	77	3.26e0	SMART
ANK	81	110	3.15e-7	SMART
ANK	114	143	9.05e-8	SMART
ANK	147	175	4.67e-1	SMART
ANK	176	205	1.42e0	SMART
ANK	209	238	4.39e-6	SMART
ANK	242	271	1.33e-5	SMART
ANK	275	304	7.53e-5	SMART
ANK	308	337	2.35e-6	SMART
ANK	341	370	6.65e-6	SMART
ANK	374	403	5.2e-8	SMART
ANK	407	436	8.78e-6	SMART
ANK	440	469	7.53e-5	SMART
ANK	473	502	5.49e-7	SMART
ANK	506	535	2.58e-3	SMART
ANK	539	568	1.88e-5	SMART
ANK	572	601	1.02e-6	SMART
ANK	605	634	7.64e-6	SMART
ANK	638	669	3.23e-4	SMART
ANK	671	700	1.38e-3	SMART
ANK	704	733	1.58e-7	SMART
ANK	737	766	2.85e-5	SMART
ZU5	923	1027	1.9e-60	SMART
low complexity region	1050	1059	N/A	INTRINSIC
low complexity region	1387	1397	N/A	INTRINSIC
DEATH	1405	1499	3.21e-26	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141930

Predicted Effect

probably benign
Transcript: ENSMUST00000173248

SMART Domains

Protein: ENSMUSP00000133322
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	15	44	2.5e3	SMART
ANK	48	77	3.26e0	SMART
ANK	81	110	3.15e-7	SMART
ANK	114	143	9.05e-8	SMART
ANK	147	175	4.67e-1	SMART
ANK	176	205	1.42e0	SMART
ANK	209	238	4.39e-6	SMART
ANK	242	271	1.33e-5	SMART
ANK	275	304	7.53e-5	SMART
ANK	308	337	2.35e-6	SMART
ANK	341	370	6.65e-6	SMART
ANK	374	403	5.2e-8	SMART
ANK	407	436	8.78e-6	SMART
ANK	440	469	7.53e-5	SMART
ANK	473	502	5.49e-7	SMART
ANK	506	535	2.58e-3	SMART
ANK	539	568	1.88e-5	SMART
ANK	572	601	1.02e-6	SMART
ANK	605	634	7.64e-6	SMART
ANK	638	669	3.23e-4	SMART
ANK	671	700	1.38e-3	SMART
ANK	704	733	1.58e-7	SMART
ANK	737	766	2.85e-5	SMART
ZU5	923	1027	1.9e-60	SMART
low complexity region	1050	1059	N/A	INTRINSIC
low complexity region	1387	1397	N/A	INTRINSIC
DEATH	1405	1499	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121802

SMART Domains

Protein: ENSMUSP00000113571
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
coiled coil region	1	39	N/A	INTRINSIC
ANK	44	73	2.5e3	SMART
ANK	77	106	3.26e0	SMART
ANK	110	139	3.15e-7	SMART
ANK	143	172	9.05e-8	SMART
ANK	176	204	4.67e-1	SMART
ANK	205	234	1.42e0	SMART
ANK	238	267	4.39e-6	SMART
ANK	271	300	1.33e-5	SMART
ANK	304	333	7.53e-5	SMART
ANK	337	366	2.35e-6	SMART
ANK	370	399	6.65e-6	SMART
ANK	403	432	5.2e-8	SMART
ANK	436	465	8.78e-6	SMART
ANK	469	498	7.53e-5	SMART
ANK	502	531	5.49e-7	SMART
ANK	535	564	2.58e-3	SMART
ANK	568	597	1.88e-5	SMART
ANK	601	630	1.02e-6	SMART
ANK	634	663	7.64e-6	SMART
ANK	667	698	3.23e-4	SMART
ANK	700	729	1.38e-3	SMART
ANK	733	762	1.58e-7	SMART
ANK	766	795	2.85e-5	SMART
ZU5	952	1056	1.9e-60	SMART
low complexity region	1079	1088	N/A	INTRINSIC
low complexity region	1416	1426	N/A	INTRINSIC
DEATH	1434	1528	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000123418

SMART Domains

Protein: ENSMUSP00000121785
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	15	44	2.5e3	SMART
ANK	48	77	3.26e0	SMART
ANK	81	110	3.15e-7	SMART
ANK	114	143	9.05e-8	SMART
ANK	147	175	4.67e-1	SMART
ANK	176	205	1.42e0	SMART
ANK	209	238	4.39e-6	SMART
ANK	242	271	1.33e-5	SMART
ANK	275	304	7.53e-5	SMART
ANK	308	337	2.35e-6	SMART
ANK	341	370	6.65e-6	SMART
ANK	374	403	5.2e-8	SMART
ANK	407	436	8.78e-6	SMART
ANK	440	469	7.53e-5	SMART
ANK	473	502	5.49e-7	SMART
ANK	506	535	2.58e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000173573

SMART Domains

Protein: ENSMUSP00000133901
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	15	44	2.5e3	SMART
ANK	48	77	3.26e0	SMART
ANK	81	110	3.15e-7	SMART
ANK	114	143	9.05e-8	SMART
ANK	147	175	4.67e-1	SMART
ANK	176	205	1.42e0	SMART
ANK	209	238	4.39e-6	SMART
ANK	242	271	1.33e-5	SMART
ANK	275	304	7.53e-5	SMART
ANK	308	337	2.35e-6	SMART
ANK	341	370	6.65e-6	SMART
ANK	374	403	5.2e-8	SMART
ANK	407	436	8.78e-6	SMART
ANK	440	469	7.53e-5	SMART
ANK	473	502	5.49e-7	SMART
ANK	506	535	2.58e-3	SMART
ANK	539	568	1.88e-5	SMART
ANK	572	601	1.02e-6	SMART
ANK	605	634	7.64e-6	SMART
ANK	638	669	3.23e-4	SMART
ANK	671	700	1.38e-3	SMART
ANK	704	733	1.58e-7	SMART
ANK	737	766	2.85e-5	SMART
ZU5	923	1027	1.9e-60	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000141784

SMART Domains

Protein: ENSMUSP00000117966
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
ANK	7	36	2.5e3	SMART
ANK	40	69	3.26e0	SMART
ANK	73	102	3.15e-7	SMART
ANK	106	135	9.05e-8	SMART
ANK	139	167	4.67e-1	SMART
ANK	168	197	1.42e0	SMART
ANK	201	230	4.39e-6	SMART
ANK	234	263	1.33e-5	SMART
ANK	267	296	7.53e-5	SMART
ANK	300	329	2.35e-6	SMART
ANK	333	362	6.65e-6	SMART
ANK	366	395	5.2e-8	SMART
ANK	399	428	8.78e-6	SMART
ANK	432	461	7.53e-5	SMART
ANK	465	494	5.49e-7	SMART
ANK	498	527	2.58e-3	SMART
ANK	531	560	1.88e-5	SMART
ANK	564	593	1.02e-6	SMART
ANK	597	626	7.64e-6	SMART
ANK	630	661	3.23e-4	SMART
ANK	663	692	1.38e-3	SMART
ANK	696	725	1.58e-7	SMART
ANK	729	758	2.85e-5	SMART
ZU5	907	1011	1.9e-60	SMART
low complexity region	1034	1043	N/A	INTRINSIC
low complexity region	1371	1381	N/A	INTRINSIC
DEATH	1389	1483	3.21e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121075

SMART Domains

Protein: ENSMUSP00000112966
Gene: ENSMUSG00000031543

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC

Meta Mutation Damage Score

0.0649

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.4%
20x: 92.8%

Validation Efficiency

97% (76/78)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit a significantly decreased number of gastrin-producing (G) cells in the stomach antrum, hypogastrinemia, and increased stomach luminal pH, with a corresponding increase in antral somatostatin-producing (D) cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 121,899,098 (GRCm39)	N693K	probably damaging	Het
Adgrl3	T	A	5: 81,841,454 (GRCm39)	V845D	probably damaging	Het
Anxa8	T	A	14: 33,819,896 (GRCm39)	F269Y	probably benign	Het
Anxa8	T	A	14: 33,819,897 (GRCm39)	F295L	possibly damaging	Het
Aox4	C	G	1: 58,268,025 (GRCm39)	L202V	probably damaging	Het
Ap3s2	A	G	7: 79,559,617 (GRCm39)	V104A	probably benign	Het
Aqp3	G	A	4: 41,094,843 (GRCm39)		probably benign	Het
Arhgef26	C	T	3: 62,355,680 (GRCm39)	H751Y	probably benign	Het
Asic4	A	T	1: 75,446,331 (GRCm39)	Q291L	possibly damaging	Het
Bap1	T	C	14: 30,978,681 (GRCm39)	Y31H	probably damaging	Het
Brf1	A	T	12: 112,924,759 (GRCm39)	V655D	probably damaging	Het
Cebpz	A	G	17: 79,238,820 (GRCm39)	S663P	probably benign	Het
Ces2h	A	G	8: 105,744,693 (GRCm39)	D357G	probably benign	Het
Cfap36	T	C	11: 29,194,073 (GRCm39)	T90A	probably benign	Het
Ciita	A	T	16: 10,330,134 (GRCm39)	D803V	probably damaging	Het
Clnk	C	A	5: 38,931,951 (GRCm39)		probably benign	Het
Cyp46a1	A	G	12: 108,317,470 (GRCm39)	N158S	probably damaging	Het
Cyp4f13	A	G	17: 33,160,080 (GRCm39)	I98T	possibly damaging	Het
Def8	G	A	8: 124,183,234 (GRCm39)	A278T	probably damaging	Het
Dll3	T	A	7: 28,000,746 (GRCm39)	D103V	possibly damaging	Het
Dnai1	T	A	4: 41,629,814 (GRCm39)	M446K	possibly damaging	Het
Dppa4	A	T	16: 48,111,425 (GRCm39)	T85S	probably benign	Het
Eif4g1	A	T	16: 20,494,095 (GRCm39)	H57L	probably damaging	Het
Fmnl3	G	C	15: 99,220,619 (GRCm39)		probably benign	Het
Fn1	T	A	1: 71,663,269 (GRCm39)	Q1073L	possibly damaging	Het
Foxp4	T	C	17: 48,180,104 (GRCm39)	D599G	unknown	Het
Frrs1	T	C	3: 116,675,456 (GRCm39)	V128A	possibly damaging	Het
Gcfc2	G	A	6: 81,926,935 (GRCm39)	D608N	probably damaging	Het
Gm1043	T	C	5: 37,350,317 (GRCm39)		probably benign	Het
Gm5148	T	C	3: 37,768,926 (GRCm39)	E98G	probably benign	Het
Gpr141	T	C	13: 19,936,428 (GRCm39)	I116V	probably benign	Het
Hic1	T	C	11: 75,058,169 (GRCm39)	N240S	probably damaging	Het
Hpx	G	A	7: 105,241,445 (GRCm39)	T322I	probably damaging	Het
Hs3st4	A	T	7: 123,996,416 (GRCm39)	M361L	probably benign	Het
Ifrd1	A	G	12: 40,257,129 (GRCm39)		probably benign	Het
Ino80	G	A	2: 119,213,441 (GRCm39)	R1249C	probably damaging	Het
Klk1b21	T	A	7: 43,755,319 (GRCm39)	C173S	probably damaging	Het
Krt25	A	T	11: 99,213,524 (GRCm39)	V65E	probably benign	Het
Lrrc15	A	T	16: 30,092,267 (GRCm39)	D357E	possibly damaging	Het
Lrrd1	T	A	5: 3,901,345 (GRCm39)	V550E	probably benign	Het
Macf1	A	G	4: 123,334,540 (GRCm39)	Y1490H	probably damaging	Het
Macrod1	A	G	19: 7,174,281 (GRCm39)		probably benign	Het
Mcm5	T	A	8: 75,847,508 (GRCm39)	V435D	probably damaging	Het
Mctp1	C	T	13: 76,975,831 (GRCm39)	R478C	probably damaging	Het
Med10	T	C	13: 69,959,817 (GRCm39)		probably benign	Het
Mrpl4	T	C	9: 20,919,888 (GRCm39)	Y280H	probably benign	Het
Msrb3	T	C	10: 120,687,892 (GRCm39)	E61G	probably damaging	Het
Myo1c	T	C	11: 75,551,827 (GRCm39)	Y337H	possibly damaging	Het
Myo7b	T	A	18: 32,143,204 (GRCm39)	T165S	probably damaging	Het
Myrfl	T	A	10: 116,685,138 (GRCm39)	R81W	probably damaging	Het
Neil1	T	C	9: 57,051,030 (GRCm39)		probably benign	Het
Neto2	A	G	8: 86,367,673 (GRCm39)	I357T	possibly damaging	Het
Nfat5	C	T	8: 108,065,707 (GRCm39)	R156W	probably damaging	Het
Or52h7	A	T	7: 104,214,210 (GRCm39)	I261L	probably benign	Het
Plce1	T	C	19: 38,512,863 (GRCm39)	I54T	possibly damaging	Het
Prex2	A	T	1: 11,355,267 (GRCm39)		probably benign	Het
Psapl1	T	A	5: 36,361,975 (GRCm39)	V189E	probably damaging	Het
Ptdss2	T	G	7: 140,735,232 (GRCm39)		probably benign	Het
Rnf213	T	C	11: 119,307,322 (GRCm39)	C661R	probably benign	Het
Rpap1	T	C	2: 119,595,380 (GRCm39)		probably null	Het
Rrp1b	A	G	17: 32,279,426 (GRCm39)	T696A	probably benign	Het
Sacm1l	T	A	9: 123,377,982 (GRCm39)	H87Q	probably benign	Het
Serpinb11	T	A	1: 107,305,260 (GRCm39)	M212K	probably damaging	Het
Tbc1d22a	C	A	15: 86,183,885 (GRCm39)	T248K	probably damaging	Het
Tcerg1	C	T	18: 42,701,679 (GRCm39)		probably benign	Het
Tpst1	T	A	5: 130,130,627 (GRCm39)	H32Q	probably damaging	Het
Tsc2	A	T	17: 24,818,600 (GRCm39)	V1412E	possibly damaging	Het
Usp19	C	A	9: 108,378,514 (GRCm39)	P1326Q	possibly damaging	Het
Vmn1r235	T	A	17: 21,482,596 (GRCm39)	M307K	probably damaging	Het
Vmn2r58	T	A	7: 41,487,048 (GRCm39)	T616S	probably damaging	Het
Vps13a	G	A	19: 16,637,863 (GRCm39)	T2406I	possibly damaging	Het
Zbtb26	T	A	2: 37,326,053 (GRCm39)	M328L	probably benign	Het
Zp2	A	G	7: 119,736,423 (GRCm39)	F340S	probably damaging	Het

Other mutations in Nkx6-3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0360:Nkx6-3	UTSW	8	23,647,722 (GRCm39)	missense	possibly damaging	0.93
R0364:Nkx6-3	UTSW	8	23,647,722 (GRCm39)	missense	possibly damaging	0.93
R3954:Nkx6-3	UTSW	8	23,643,742 (GRCm39)	missense	possibly damaging	0.53
R4724:Nkx6-3	UTSW	8	23,646,285 (GRCm39)	missense	probably damaging	1.00
R4885:Nkx6-3	UTSW	8	23,643,914 (GRCm39)	missense	possibly damaging	0.95
R4969:Nkx6-3	UTSW	8	23,647,725 (GRCm39)	missense	probably damaging	1.00
R5165:Nkx6-3	UTSW	8	23,643,759 (GRCm39)	missense	probably damaging	1.00
R6220:Nkx6-3	UTSW	8	23,643,987 (GRCm39)	critical splice donor site	probably null
R6262:Nkx6-3	UTSW	8	23,643,863 (GRCm39)	missense	probably benign	0.34
R6894:Nkx6-3	UTSW	8	23,647,632 (GRCm39)	missense	probably benign	0.41
R7440:Nkx6-3	UTSW	8	23,643,770 (GRCm39)	missense	probably damaging	1.00
R8515:Nkx6-3	UTSW	8	23,643,707 (GRCm39)	missense	possibly damaging	0.83
R8841:Nkx6-3	UTSW	8	23,646,274 (GRCm39)	missense	probably damaging	1.00
R9439:Nkx6-3	UTSW	8	23,643,778 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCTCTGAGTATGGAAAGCACCCC -3'
(R):5'- TTCCAGCCTTGGAAAAGCTCCC -3'

Sequencing Primer
(F):5'- GTATGGAAAGCACCCCTCCTTAG -3'
(R):5'- TTGGAAAAGCTCCCCACCTG -3'

Posted On

2013-04-12