Mutagenetix > Incidental Mutation

Incidental Mutation 'R2033:Gpr155'

221442

Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

PGR22, 1110017O10Rik, DEPDC3, F730029F15Rik

MMRRC Submission

040040-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2033 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73341506-73386572 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 73348182 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 726 (H726L)

Ref Sequence

ENSEMBL: ENSMUSP00000107675 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000048504

SMART Domains

Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	1.9e-16	PFAM
Pfam:Mem_trans	187	360	4.9e-12	PFAM
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000076463
AA Change: H754L

PolyPhen 2 Score 0.176 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: H754L

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112043
AA Change: H754L

PolyPhen 2 Score 0.176 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: H754L

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112044
AA Change: H726L

PolyPhen 2 Score 0.176 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: H726L

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000131430
AA Change: H194L

SMART Domains

Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
AA Change: H194L

Domain	Start	End	E-Value	Type
transmembrane domain	98	120	N/A	INTRINSIC
transmembrane domain	130	152	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184503

Meta Mutation Damage Score

0.0858

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.5%

Validation Efficiency

96% (53/55)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	T	C	4: 144,456,383	T172A	probably benign	Het
Atp6v1c1	T	C	15: 38,673,966		probably null	Het
Bpifc	G	A	10: 86,000,632	T3I	possibly damaging	Het
Car12	A	G	9: 66,717,558		probably null	Het
Ccrl2	A	G	9: 111,055,870	F187L	possibly damaging	Het
Cep250	G	A	2: 155,970,892	R544H	probably damaging	Het
Col4a3	T	G	1: 82,718,011		probably benign	Het
Cyb5r2	T	C	7: 107,756,907		probably null	Het
Elfn2	A	G	15: 78,671,896	V817A	probably damaging	Het
Eln	C	T	5: 134,710,106		probably null	Het
Eml5	T	C	12: 98,791,386	E1896G	possibly damaging	Het
G530012D18Rik	CACAGA	CA	1: 85,577,154		probably null	Het
Galnt11	C	T	5: 25,247,538	T16I	probably damaging	Het
Gars	C	T	6: 55,077,723	H672Y	probably benign	Het
Inpp1	T	A	1: 52,790,173	N229I	possibly damaging	Het
Isg20	A	C	7: 78,916,533	I77L	probably damaging	Het
Kit	G	C	5: 75,637,317	D422H	possibly damaging	Het
Lonp2	A	G	8: 86,708,942	E602G	possibly damaging	Het
Mink1	T	C	11: 70,612,508	V1143A	probably damaging	Het
Myh6	A	C	14: 54,963,645	L120R	probably benign	Het
Myo18a	T	A	11: 77,843,099		probably null	Het
Nphs2	T	C	1: 156,323,738	V249A	probably damaging	Het
Npsr1	A	G	9: 24,313,352	K342E	probably benign	Het
Nrros	C	T	16: 32,144,157	W311*	probably null	Het
Nudt18	G	T	14: 70,579,616	G162V	possibly damaging	Het
Odam	A	G	5: 87,892,419	D248G	probably benign	Het
Olfr1224-ps1	A	G	2: 89,157,154	V7A	probably damaging	Het
Olfr147	G	T	9: 38,403,373	M166I	probably damaging	Het
Olfr390	T	C	11: 73,787,438	S167P	probably benign	Het
Olfr557	A	G	7: 102,699,162	E308G	probably benign	Het
Olfr572	T	C	7: 102,928,408	V260A	probably benign	Het
Pde4b	G	T	4: 102,605,295	D723Y	probably benign	Het
Pdzrn3	T	C	6: 101,150,954	E917G	probably damaging	Het
Ppip5k1	C	T	2: 121,337,627	R715H	probably damaging	Het
Prkdc	T	A	16: 15,687,352		probably benign	Het
Ptp4a3	A	G	15: 73,753,769	Y21C	probably damaging	Het
Ptprk	C	A	10: 28,592,767		probably benign	Het
Rfesd	C	A	13: 76,002,872		probably null	Het
Rtel1	A	T	2: 181,351,863	K592*	probably null	Het
Siah1a	T	A	8: 86,725,270	K195N	probably damaging	Het
Slc5a5	G	T	8: 70,888,587	D369E	probably damaging	Het
Slc6a6	A	T	6: 91,724,910	I100F	probably benign	Het
Smtn	T	C	11: 3,517,781	I913V	probably benign	Het
Stk17b	A	G	1: 53,761,076	S248P	probably damaging	Het
Sun1	C	T	5: 139,225,438	H149Y	probably damaging	Het
Taar5	T	C	10: 23,971,094	I130T	possibly damaging	Het
Tmem132b	G	T	5: 125,749,289	V448F	probably damaging	Het
Tmem94	C	A	11: 115,794,328	N888K	possibly damaging	Het
Trpc1	T	C	9: 95,706,843	N742S	probably damaging	Het
Ttbk2	T	C	2: 120,806,849	T112A	probably damaging	Het
Tubb2a	A	T	13: 34,075,456	L117Q	probably damaging	Het
Vmn1r60	T	A	7: 5,544,820	M94L	probably benign	Het
Vmn2r83	A	G	10: 79,491,819	T754A	probably benign	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73362613	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73351885	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73362423	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73382232	missense	probably benign
IGL01733:Gpr155	APN	2	73353612	splice site	probably null
IGL03342:Gpr155	APN	2	73349678	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73370138	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73348263	missense	probably benign	0.08
R0226:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R0399:Gpr155	UTSW	2	73370002	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73370144	splice site	probably benign
R1570:Gpr155	UTSW	2	73370038	missense	possibly damaging	0.87
R1598:Gpr155	UTSW	2	73370090	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73364164	splice site	probably null
R1648:Gpr155	UTSW	2	73364164	splice site	probably null
R1756:Gpr155	UTSW	2	73367577	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73381935	missense	probably damaging	1.00
R2044:Gpr155	UTSW	2	73373633	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73356658	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73382127	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73348135	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73368392	nonsense	probably null
R3924:Gpr155	UTSW	2	73370076	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73367538	nonsense	probably null
R4950:Gpr155	UTSW	2	73382185	missense	probably benign
R5337:Gpr155	UTSW	2	73348248	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73370089	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73343718	nonsense	probably null
R5929:Gpr155	UTSW	2	73373667	nonsense	probably null
R6293:Gpr155	UTSW	2	73373997	missense	possibly damaging	0.47
R6553:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R6585:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R7003:Gpr155	UTSW	2	73343617	missense	probably damaging	0.99
R7353:Gpr155	UTSW	2	73367491	nonsense	probably null
R7506:Gpr155	UTSW	2	73368339	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73382947	intron	probably benign
R7753:Gpr155	UTSW	2	73382206	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73381952	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73381985	nonsense	probably null
R7815:Gpr155	UTSW	2	73362560	missense	probably benign
R7873:Gpr155	UTSW	2	73343590	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73343462	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73343695	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73373649	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73373765	missense	possibly damaging	0.78
R9683:Gpr155	UTSW	2	73362436	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACGCTAATGGCCGACATGTAG -3'
(R):5'- GGTTCAGCGATCAATGAAATCCC -3'

Sequencing Primer
(F):5'- CCGACATGTAGAGAATGTTAATCGCC -3'
(R):5'- TGAAATCCCCATGTGGATGAC -3'

Posted On

2014-08-25