Mutagenetix > Incidental Mutation

Incidental Mutation 'R1977:Or2l5'

221914

Institutional Source

Beutler Lab

Gene Symbol

Or2l5

Ensembl Gene

ENSMUSG00000045341

Gene Name

olfactory receptor family 2 subfamily L member 5

Synonyms

MOR272-1, GA_x54KRFPKG5P-15963726-15962788, Olfr167

MMRRC Submission

039990-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.248) question?

Stock #

R1977 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19333446-19334384 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 19333586 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 267 (P267S)

Ref Sequence

ENSEMBL: ENSMUSP00000150102 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054606] [ENSMUST00000214315]

AlphaFold

Q8VGJ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000054606
AA Change: P267S

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000055344
Gene: ENSMUSG00000045341
AA Change: P267S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	306	4.8e-46	PFAM
Pfam:7TM_GPCR_Srsx	33	295	1.2e-6	PFAM
Pfam:7tm_1	40	289	1.8e-26	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214315
AA Change: P267S

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217566

Meta Mutation Damage Score

0.5034

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsf3	G	T	8: 123,508,272 (GRCm39)	C256F	probably damaging	Het
Adgra2	T	A	8: 27,605,789 (GRCm39)	V647D	possibly damaging	Het
AI593442	A	T	9: 52,589,492 (GRCm39)	S28R	probably damaging	Het
Akr1c21	G	C	13: 4,624,211 (GRCm39)	G22R	probably damaging	Het
Ampd3	T	C	7: 110,402,369 (GRCm39)	W458R	probably damaging	Het
Arhgap23	G	T	11: 97,342,273 (GRCm39)	R185L	possibly damaging	Het
Arhgap45	A	T	10: 79,856,652 (GRCm39)	I67F	probably damaging	Het
Asah1	A	G	8: 41,796,554 (GRCm39)		probably null	Het
Atl2	A	G	17: 80,160,019 (GRCm39)	Y56H	probably damaging	Het
Carf	A	T	1: 60,185,295 (GRCm39)	I447F	probably damaging	Het
Crmp1	A	G	5: 37,433,627 (GRCm39)	N162S	probably damaging	Het
Cyp2a5	A	G	7: 26,535,347 (GRCm39)	E103G	probably benign	Het
Cyp2c40	T	C	19: 39,766,485 (GRCm39)	D370G	probably damaging	Het
Dhrs2	T	A	14: 55,472,112 (GRCm39)	M1K	probably null	Het
Dnah17	T	C	11: 118,003,417 (GRCm39)	E810G	possibly damaging	Het
E2f5	T	A	3: 14,652,416 (GRCm39)	I84N	probably damaging	Het
Eif2ak4	A	T	2: 118,292,238 (GRCm39)	K1185*	probably null	Het
Eif4ebp1	T	A	8: 27,765,129 (GRCm39)	M115K	probably damaging	Het
Evi5	A	T	5: 107,947,005 (GRCm39)	L505*	probably null	Het
Fbxw25	T	A	9: 109,481,924 (GRCm39)	Y254F	possibly damaging	Het
Gm7964	T	A	7: 83,406,560 (GRCm39)	F439Y	possibly damaging	Het
Gps1	A	G	11: 120,676,652 (GRCm39)	T124A	probably damaging	Het
Hopx	T	C	5: 77,265,463 (GRCm39)		probably benign	Het
Hoxd3	A	G	2: 74,574,620 (GRCm39)	S89G	possibly damaging	Het
Hrc	A	T	7: 44,985,638 (GRCm39)	D263V	probably damaging	Het
Hs6st3	T	C	14: 119,375,888 (GRCm39)	I21T	probably benign	Het
Izumo4	A	G	10: 80,538,955 (GRCm39)	Y106C	probably damaging	Het
Lama2	A	T	10: 26,866,796 (GRCm39)		probably null	Het
Lcorl	A	C	5: 45,932,762 (GRCm39)	S123R	probably null	Het
Lgr4	A	T	2: 109,842,273 (GRCm39)	I729F	probably damaging	Het
Lonp1	T	C	17: 56,922,068 (GRCm39)	T771A	possibly damaging	Het
Matn3	T	A	12: 9,011,110 (GRCm39)		probably benign	Het
Mdc1	T	A	17: 36,161,822 (GRCm39)	S912T	probably benign	Het
Mgam	G	A	6: 40,641,814 (GRCm39)	V556I	probably benign	Het
Myom2	A	T	8: 15,135,263 (GRCm39)	I489F	possibly damaging	Het
Nfatc2	G	A	2: 168,346,379 (GRCm39)	T905I	possibly damaging	Het
Nme6	G	A	9: 109,664,409 (GRCm39)	R6Q	probably damaging	Het
Nr1h5	A	G	3: 102,855,133 (GRCm39)	S323P	probably damaging	Het
Nr4a3	C	A	4: 48,056,539 (GRCm39)	R364S	probably damaging	Het
Obox7	A	T	7: 14,398,323 (GRCm39)	D79V	probably damaging	Het
Or1ab2	G	A	8: 72,863,698 (GRCm39)	G96D	probably benign	Het
Or2a51	T	A	6: 43,178,914 (GRCm39)	V112D	possibly damaging	Het
Pan2	T	C	10: 128,156,282 (GRCm39)	V1171A	probably damaging	Het
Parp8	T	A	13: 117,047,449 (GRCm39)	I208F	probably damaging	Het
Pdgfc	C	T	3: 81,116,552 (GRCm39)	T302I	probably damaging	Het
Pnpt1	A	G	11: 29,091,256 (GRCm39)	I337V	probably benign	Het
Polk	T	C	13: 96,625,736 (GRCm39)	E436G	probably damaging	Het
Pramel7	A	G	2: 87,321,465 (GRCm39)	V190A	probably benign	Het
Rplp2	T	C	7: 141,028,694 (GRCm39)		probably benign	Het
Sec23ip	T	A	7: 128,367,997 (GRCm39)	S670T	probably damaging	Het
Sgk2	A	G	2: 162,846,080 (GRCm39)	N207S	probably benign	Het
Sh2d2a	C	T	3: 87,759,123 (GRCm39)	Q242*	probably null	Het
Sh3pxd2b	A	C	11: 32,372,138 (GRCm39)	N435T	probably damaging	Het
Slc36a4	T	A	9: 15,645,506 (GRCm39)	V311D	probably damaging	Het
Stx17	G	A	4: 48,181,553 (GRCm39)	V241M	probably benign	Het
Taok3	A	T	5: 117,403,989 (GRCm39)	K721N	probably damaging	Het
Tbxas1	A	G	6: 38,925,575 (GRCm39)		probably benign	Het
Tmem87a	G	A	2: 120,204,985 (GRCm39)	A377V	probably benign	Het
Topaz1	C	T	9: 122,576,427 (GRCm39)	T6M	unknown	Het
Tspan8	A	G	10: 115,680,035 (GRCm39)	I217V	probably benign	Het
Vmn2r125	A	G	4: 156,707,162 (GRCm39)		probably null	Het
Vmn2r5	C	T	3: 64,411,642 (GRCm39)	E309K	probably damaging	Het
Wdr59	A	T	8: 112,185,270 (GRCm39)	C888S	probably benign	Het

Other mutations in Or2l5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01535:Or2l5	APN	16	19,334,228 (GRCm39)	missense	probably benign	0.01
IGL02115:Or2l5	APN	16	19,333,853 (GRCm39)	missense	probably damaging	1.00
IGL02562:Or2l5	APN	16	19,333,714 (GRCm39)	missense	possibly damaging	0.74
BB008:Or2l5	UTSW	16	19,334,258 (GRCm39)	missense	possibly damaging	0.94
BB018:Or2l5	UTSW	16	19,334,258 (GRCm39)	missense	possibly damaging	0.94
R0366:Or2l5	UTSW	16	19,333,598 (GRCm39)	missense	probably benign	0.13
R0673:Or2l5	UTSW	16	19,334,146 (GRCm39)	missense	probably damaging	1.00
R1187:Or2l5	UTSW	16	19,333,796 (GRCm39)	missense	probably benign	0.01
R1237:Or2l5	UTSW	16	19,334,375 (GRCm39)	missense	probably benign	0.01
R1975:Or2l5	UTSW	16	19,333,586 (GRCm39)	missense	probably damaging	0.99
R1997:Or2l5	UTSW	16	19,333,792 (GRCm39)	missense	probably damaging	1.00
R2225:Or2l5	UTSW	16	19,333,996 (GRCm39)	missense	probably benign	0.05
R2226:Or2l5	UTSW	16	19,333,996 (GRCm39)	missense	probably benign	0.05
R4078:Or2l5	UTSW	16	19,333,982 (GRCm39)	missense	possibly damaging	0.76
R5019:Or2l5	UTSW	16	19,334,285 (GRCm39)	missense	probably damaging	0.99
R5071:Or2l5	UTSW	16	19,333,777 (GRCm39)	missense	probably benign	0.00
R5278:Or2l5	UTSW	16	19,334,128 (GRCm39)	nonsense	probably null
R5415:Or2l5	UTSW	16	19,333,996 (GRCm39)	missense	possibly damaging	0.94
R5744:Or2l5	UTSW	16	19,334,086 (GRCm39)	missense	probably benign	0.00
R5991:Or2l5	UTSW	16	19,333,507 (GRCm39)	missense	probably damaging	0.99
R6151:Or2l5	UTSW	16	19,334,281 (GRCm39)	missense	probably damaging	1.00
R6540:Or2l5	UTSW	16	19,333,571 (GRCm39)	missense	probably benign
R7014:Or2l5	UTSW	16	19,334,206 (GRCm39)	missense	probably benign	0.20
R7145:Or2l5	UTSW	16	19,333,649 (GRCm39)	missense	probably damaging	0.99
R7535:Or2l5	UTSW	16	19,333,544 (GRCm39)	missense	probably damaging	1.00
R7677:Or2l5	UTSW	16	19,333,678 (GRCm39)	missense	probably benign	0.42
R7715:Or2l5	UTSW	16	19,333,480 (GRCm39)	missense	probably benign	0.00
R7931:Or2l5	UTSW	16	19,334,258 (GRCm39)	missense	possibly damaging	0.94
R8137:Or2l5	UTSW	16	19,333,846 (GRCm39)	missense	possibly damaging	0.89
R8671:Or2l5	UTSW	16	19,333,804 (GRCm39)	missense	possibly damaging	0.63

Predicted Primers

PCR Primer
(F):5'- TGCATAAATTCTGGATTCACGG -3'
(R):5'- GCACAGACACATGGGTCTATGAG -3'

Sequencing Primer
(F):5'- CTGGATTCACGGAAGCAACTTGTC -3'
(R):5'- CACATGGGTCTATGAGTATACAGTG -3'

Posted On

2014-08-25