Mutagenetix > Incidental Mutations

Incidental Mutation 'R1978:Olfr1328'

221974

Institutional Source

Beutler Lab

Gene Symbol

Olfr1328

Ensembl Gene

ENSMUSG00000111259

Gene Name

olfactory receptor 1328

Synonyms

Olfr1519, MOR259-1, MOR259-13, MOR259-1, GA_x6K02T2QD9B-18602750-18603691

MMRRC Submission

039991-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R1978 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

118930071-118938612 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 118934184 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 221 (Y221*)

Ref Sequence

ENSEMBL: ENSMUSP00000149039 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081960] [ENSMUST00000215312]

Predicted Effect

probably null
Transcript: ENSMUST00000081960
AA Change: Y219*

SMART Domains

Protein: ENSMUSP00000080626
Gene: ENSMUSG00000111259
AA Change: Y219*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	1.1e-53	PFAM
Pfam:7TM_GPCR_Srsx	36	306	9.1e-8	PFAM
Pfam:7tm_1	42	291	1.7e-26	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215312
AA Change: Y221*

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	T	A	5: 63,898,537	C205*	probably null	Het
2810474O19Rik	T	A	6: 149,326,432	N325K	probably damaging	Het
4921539E11Rik	T	A	4: 103,270,764	T55S	possibly damaging	Het
Akap12	A	G	10: 4,313,855	D88G	probably benign	Het
Ankrd53	C	A	6: 83,763,203	F84L	probably damaging	Het
Apol7b	A	C	15: 77,423,339	F319V	probably damaging	Het
Bsn	A	G	9: 108,114,549	S1335P	probably benign	Het
Cep192	T	C	18: 67,803,158		probably null	Het
Cfap57	A	G	4: 118,593,132	S598P	probably benign	Het
Commd8	T	C	5: 72,165,499	H25R	probably damaging	Het
Crisp4	T	C	1: 18,128,665	I143V	probably benign	Het
Cyp4a12b	A	T	4: 115,438,145	T483S	probably benign	Het
Dbx2	C	T	15: 95,632,353	M244I	probably damaging	Het
Dnah6	T	G	6: 73,121,970	H1982P	possibly damaging	Het
Fam220a	C	A	5: 143,563,127	P98Q	probably damaging	Het
Ggnbp1	A	G	17: 27,029,543	K29E	possibly damaging	Het
Gm14569	A	C	X: 36,432,128	M976R	probably benign	Het
Gm9573	T	A	17: 35,622,965		probably benign	Het
Hck	G	T	2: 153,129,856	W112C	probably damaging	Het
Heatr5a	A	T	12: 51,939,658	S591T	possibly damaging	Het
Hhat	A	G	1: 192,717,107	S242P	probably benign	Het
Hnrnpll	A	G	17: 80,044,518	S333P	probably benign	Het
Hoxc6	T	C	15: 103,010,007		probably null	Het
Inpp5j	G	A	11: 3,502,150	P367S	probably damaging	Het
Lamc2	A	G	1: 153,133,597		probably null	Het
Loxhd1	T	A	18: 77,321,642	I194N	possibly damaging	Het
Miga1	CCAGGGCAG	CCAG	3: 152,335,304		probably null	Het
Mkln1	C	T	6: 31,490,530	Q60*	probably null	Het
Mybph	C	A	1: 134,196,996	H185N	probably benign	Het
Myo1g	T	C	11: 6,520,829	D9G	possibly damaging	Het
Myo6	A	T	9: 80,228,925	D110V	probably damaging	Het
Ncoa7	A	G	10: 30,691,299	V412A	probably benign	Het
Neb	T	C	2: 52,287,345	K1328R	probably damaging	Het
Olfm5	T	A	7: 104,164,741	Q22L	unknown	Het
Olfr1106	C	T	2: 87,048,835	V134M	possibly damaging	Het
Olfr1355	A	G	10: 78,879,280	Y36C	probably damaging	Het
Olfr1414	C	A	1: 92,511,777	G84C	probably damaging	Het
Olfr1423	T	C	19: 12,036,341	T134A	probably benign	Het
Olfr414	T	A	1: 174,431,091	I221N	probably damaging	Het
P3h1	A	T	4: 119,247,976	Q717L	probably null	Het
Pclo	T	C	5: 14,713,795	I4094T	unknown	Het
Pfdn6	G	A	17: 33,939,077	R73W	probably benign	Het
Phyhipl	A	C	10: 70,559,761	M205R	possibly damaging	Het
Pitpnm1	C	T	19: 4,107,973		probably null	Het
Plcg1	A	G	2: 160,752,578		probably null	Het
Pnldc1	A	G	17: 12,906,505	S81P	possibly damaging	Het
Pno1	T	C	11: 17,204,519	I221V	possibly damaging	Het
Porcn	A	G	X: 8,204,301	V75A	probably damaging	Het
Prkcg	T	A	7: 3,305,346	C69S	probably damaging	Het
Rbbp6	G	T	7: 122,999,488		probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,110,324		probably benign	Het
Scly	A	G	1: 91,320,169	D413G	probably damaging	Het
Scn11a	G	A	9: 119,780,795	R996*	probably null	Het
Slc6a13	A	T	6: 121,332,373	D281V	probably damaging	Het
Slfn5	T	C	11: 82,956,616	V109A	probably benign	Het
Smyd1	A	T	6: 71,312,719		probably null	Het
Snx29	T	A	16: 11,367,724	M57K	probably benign	Het
Stag1	T	G	9: 100,888,086	I603S	probably benign	Het
Svep1	C	A	4: 58,097,292	C1417F	possibly damaging	Het
Tbc1d23	T	C	16: 57,189,351	I392V	probably benign	Het
Tchh	T	C	3: 93,446,799	L1182P	unknown	Het
Tle3	T	A	9: 61,394,633	V108E	probably damaging	Het
Tmem144	T	C	3: 79,825,400		probably null	Het
Tpr	T	G	1: 150,419,907	L894V	possibly damaging	Het
Trappc9	A	T	15: 73,000,025	V472E	probably damaging	Het
Trim38	T	C	13: 23,791,098	V340A	probably damaging	Het
Ttc37	T	C	13: 76,134,815	V752A	probably benign	Het
Ttn	A	T	2: 76,811,243	L5176Q	possibly damaging	Het
Vmn1r12	T	A	6: 57,159,509	I197N	possibly damaging	Het
Vmn1r26	T	C	6: 58,009,126	Y26C	possibly damaging	Het
Vwa3a	A	G	7: 120,758,954	I83V	probably null	Het
Xirp1	C	T	9: 120,018,591	E409K	probably benign	Het
Zc3h14	T	G	12: 98,763,922	I46R	probably damaging	Het
Zfp976	A	G	7: 42,613,841	C191R	probably damaging	Het

Other mutations in Olfr1328

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02491:Olfr1328	APN	4	118934161	missense	probably damaging	1.00
IGL02685:Olfr1328	APN	4	118933937	missense	possibly damaging	0.61
IGL02886:Olfr1328	APN	4	118934830	missense	probably benign
IGL02899:Olfr1328	APN	4	118934662	missense	probably damaging	1.00
IGL02957:Olfr1328	APN	4	118934119	missense	probably damaging	1.00
PIT4453001:Olfr1328	UTSW	4	118934626	missense	probably benign
R0211:Olfr1328	UTSW	4	118934270	missense	probably benign	0.00
R0211:Olfr1328	UTSW	4	118934270	missense	probably benign	0.00
R1158:Olfr1328	UTSW	4	118934417	missense	probably damaging	1.00
R1450:Olfr1328	UTSW	4	118934510	missense	probably benign	0.01
R1682:Olfr1328	UTSW	4	118934581	missense	probably damaging	1.00
R2363:Olfr1328	UTSW	4	118934033	missense	probably benign	0.02
R2364:Olfr1328	UTSW	4	118934033	missense	probably benign	0.02
R2365:Olfr1328	UTSW	4	118934033	missense	probably benign	0.02
R2507:Olfr1328	UTSW	4	118933925	missense	probably benign
R2912:Olfr1328	UTSW	4	118934701	missense	probably benign	0.28
R3937:Olfr1328	UTSW	4	118934683	missense	probably damaging	1.00
R4058:Olfr1328	UTSW	4	118934683	missense	probably damaging	1.00
R4089:Olfr1328	UTSW	4	118934033	missense	probably benign	0.02
R4090:Olfr1328	UTSW	4	118934033	missense	probably benign	0.02
R4419:Olfr1328	UTSW	4	118934389	missense	possibly damaging	0.56
R4717:Olfr1328	UTSW	4	118934429	missense	probably benign	0.45
R5570:Olfr1328	UTSW	4	118934066	missense	possibly damaging	0.88
R5591:Olfr1328	UTSW	4	118934461	missense	probably damaging	1.00
R6149:Olfr1328	UTSW	4	118934431	missense	probably damaging	1.00
R7202:Olfr1328	UTSW	4	118934018	missense	probably benign
R7214:Olfr1328	UTSW	4	118933949	missense	possibly damaging	0.88
R7391:Olfr1328	UTSW	4	118934001	missense	possibly damaging	0.61
R7666:Olfr1328	UTSW	4	118934264	missense	probably damaging	1.00
R7676:Olfr1328	UTSW	4	118934150	missense	probably damaging	1.00
R8053:Olfr1328	UTSW	4	118934111	missense	probably damaging	1.00
R8311:Olfr1328	UTSW	4	118934150	missense	probably damaging	0.97
Z1176:Olfr1328	UTSW	4	118933918	missense	probably benign	0.19

Predicted Primers

PCR Primer
(F):5'- TGTTCCTCAGACTGTAGACCAC -3'
(R):5'- CTTCACCATGAGTCTGCCATAC -3'

Sequencing Primer
(F):5'- TGTAAAAGAGTGAGACCTGCTTGTC -3'
(R):5'- ATACTGTGGGCCCAACAGG -3'

Posted On

2014-08-25