Incidental Mutation 'R1978:Zc3h14'
| ID |
222052 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zc3h14
|
| Ensembl Gene |
ENSMUSG00000021012 |
| Gene Name |
zinc finger CCCH type containing 14 |
| Synonyms |
1700016A15Rik, 1010001P15Rik, 2700069A02Rik |
| MMRRC Submission |
039991-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R1978 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
12 |
| Chromosomal Location |
98746964-98787753 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to G
at 98763922 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Arginine
at position 46
(I46R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000152746
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000057000]
[ENSMUST00000110104]
[ENSMUST00000110105]
[ENSMUST00000221532]
|
| AlphaFold |
Q8BJ05 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000057000
AA Change: I408R
PolyPhen 2
Score 0.646 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000055879
Gene: ENSMUSG00000021012
AA Change: I408R
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
298 |
306 |
N/A |
INTRINSIC |
|
low complexity region
|
313 |
320 |
N/A |
INTRINSIC |
|
ZnF_C3H1
|
440 |
463 |
7.16e-1 |
SMART |
|
ZnF_C3H1
|
465 |
484 |
5.27e1 |
SMART |
|
ZnF_C3H1
|
520 |
542 |
5.55e0 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000110104
AA Change: I408R
PolyPhen 2
Score 0.646 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000105731
Gene: ENSMUSG00000021012
AA Change: I408R
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
298 |
306 |
N/A |
INTRINSIC |
|
low complexity region
|
313 |
320 |
N/A |
INTRINSIC |
|
ZnF_C3H1
|
465 |
488 |
7.16e-1 |
SMART |
|
ZnF_C3H1
|
490 |
509 |
5.27e1 |
SMART |
|
ZnF_C3H1
|
545 |
567 |
5.55e0 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000110105
AA Change: I408R
PolyPhen 2
Score 0.646 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000105732
Gene: ENSMUSG00000021012
AA Change: I408R
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
298 |
306 |
N/A |
INTRINSIC |
|
low complexity region
|
313 |
320 |
N/A |
INTRINSIC |
|
ZnF_C3H1
|
596 |
619 |
7.16e-1 |
SMART |
|
ZnF_C3H1
|
621 |
640 |
5.27e1 |
SMART |
|
ZnF_C3H1
|
676 |
698 |
5.55e0 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000220865
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000221532
AA Change: I46R
PolyPhen 2
Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000221576
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000222461
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000222913
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000223451
AA Change: I79R
PolyPhen 2
Score 0.902 (Sensitivity: 0.82; Specificity: 0.94)
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.4%
- 10x: 96.7%
- 20x: 93.9%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a poly(A)-binding protein that can affect gene expression and poly(A) tail length. The encoded protein may influence mRNA stability, nuclear export, and translation. [provided by RefSeq, May 2016]
PHENOTYPE: Homozygous knockout results in impaired spatial working memory, enlarged anterior lateral ventricles in the brain, small testes and reduced litter size. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 74 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 0610040J01Rik |
T |
A |
5: 63,898,537 (GRCm38) |
C205* |
probably null |
Het |
| 2810474O19Rik |
T |
A |
6: 149,326,432 (GRCm38) |
N325K |
probably benign |
Het |
| 4921539E11Rik |
T |
A |
4: 103,270,764 (GRCm38) |
T55S |
possibly damaging |
Het |
| Akap12 |
A |
G |
10: 4,313,855 (GRCm38) |
D88G |
probably benign |
Het |
| Ankrd53 |
C |
A |
6: 83,763,203 (GRCm38) |
F84L |
probably damaging |
Het |
| Apol7b |
A |
C |
15: 77,423,339 (GRCm38) |
F319V |
probably damaging |
Het |
| Bsn |
A |
G |
9: 108,114,549 (GRCm38) |
S1335P |
probably benign |
Het |
| Cep192 |
T |
C |
18: 67,803,158 (GRCm38) |
|
probably null |
Het |
| Cfap57 |
A |
G |
4: 118,593,132 (GRCm38) |
S598P |
probably benign |
Het |
| Commd8 |
T |
C |
5: 72,165,499 (GRCm38) |
H25R |
probably damaging |
Het |
| Crisp4 |
T |
C |
1: 18,128,665 (GRCm38) |
I143V |
probably benign |
Het |
| Cyp4a12b |
A |
T |
4: 115,438,145 (GRCm38) |
T483S |
probably benign |
Het |
| Dbx2 |
C |
T |
15: 95,632,353 (GRCm38) |
M244I |
probably damaging |
Het |
| Dnah6 |
T |
G |
6: 73,121,970 (GRCm38) |
H1982P |
possibly damaging |
Het |
| Fam220a |
C |
A |
5: 143,563,127 (GRCm38) |
P98Q |
probably damaging |
Het |
| Ggnbp1 |
A |
G |
17: 27,029,543 (GRCm38) |
K29E |
possibly damaging |
Het |
| Gm14569 |
A |
C |
X: 36,432,128 (GRCm38) |
M976R |
probably benign |
Het |
| Gm9573 |
T |
A |
17: 35,622,965 (GRCm38) |
|
probably benign |
Het |
| Hck |
G |
T |
2: 153,129,856 (GRCm38) |
W112C |
probably damaging |
Het |
| Heatr5a |
A |
T |
12: 51,939,658 (GRCm38) |
S591T |
possibly damaging |
Het |
| Hhat |
A |
G |
1: 192,717,107 (GRCm38) |
S242P |
probably benign |
Het |
| Hnrnpll |
A |
G |
17: 80,044,518 (GRCm38) |
S333P |
probably benign |
Het |
| Hoxc6 |
T |
C |
15: 103,010,007 (GRCm38) |
|
probably null |
Het |
| Inpp5j |
G |
A |
11: 3,502,150 (GRCm38) |
P367S |
probably damaging |
Het |
| Lamc2 |
A |
G |
1: 153,133,597 (GRCm38) |
|
probably null |
Het |
| Loxhd1 |
T |
A |
18: 77,321,642 (GRCm38) |
I194N |
possibly damaging |
Het |
| Miga1 |
CCAGGGCAG |
CCAG |
3: 152,335,304 (GRCm38) |
|
probably null |
Het |
| Mkln1 |
C |
T |
6: 31,490,530 (GRCm38) |
Q60* |
probably null |
Het |
| Mybph |
C |
A |
1: 134,196,996 (GRCm38) |
H185N |
probably benign |
Het |
| Myo1g |
T |
C |
11: 6,520,829 (GRCm38) |
D9G |
possibly damaging |
Het |
| Myo6 |
A |
T |
9: 80,228,925 (GRCm38) |
D110V |
probably damaging |
Het |
| Ncoa7 |
A |
G |
10: 30,691,299 (GRCm38) |
V412A |
probably benign |
Het |
| Neb |
T |
C |
2: 52,287,345 (GRCm38) |
K1328R |
probably damaging |
Het |
| Olfm5 |
T |
A |
7: 104,164,741 (GRCm38) |
Q22L |
unknown |
Het |
| Olfr1106 |
C |
T |
2: 87,048,835 (GRCm38) |
V134M |
possibly damaging |
Het |
| Olfr1328 |
A |
T |
4: 118,934,184 (GRCm38) |
Y221* |
probably null |
Het |
| Olfr1355 |
A |
G |
10: 78,879,280 (GRCm38) |
Y36C |
probably damaging |
Het |
| Olfr1414 |
C |
A |
1: 92,511,777 (GRCm38) |
G84C |
probably damaging |
Het |
| Olfr1423 |
T |
C |
19: 12,036,341 (GRCm38) |
T134A |
probably benign |
Het |
| Olfr414 |
T |
A |
1: 174,431,091 (GRCm38) |
I221N |
probably damaging |
Het |
| P3h1 |
A |
T |
4: 119,247,976 (GRCm38) |
Q717L |
probably null |
Het |
| Pclo |
T |
C |
5: 14,713,795 (GRCm38) |
I4094T |
unknown |
Het |
| Pfdn6 |
G |
A |
17: 33,939,077 (GRCm38) |
R73W |
probably benign |
Het |
| Phyhipl |
A |
C |
10: 70,559,761 (GRCm38) |
M205R |
possibly damaging |
Het |
| Pitpnm1 |
C |
T |
19: 4,107,973 (GRCm38) |
|
probably null |
Het |
| Plcg1 |
A |
G |
2: 160,752,578 (GRCm38) |
|
probably null |
Het |
| Pnldc1 |
A |
G |
17: 12,906,505 (GRCm38) |
S81P |
possibly damaging |
Het |
| Pno1 |
T |
C |
11: 17,204,519 (GRCm38) |
I221V |
possibly damaging |
Het |
| Porcn |
A |
G |
X: 8,204,301 (GRCm38) |
V75A |
probably damaging |
Het |
| Prkcg |
T |
A |
7: 3,305,346 (GRCm38) |
C69S |
probably damaging |
Het |
| Rbbp6 |
G |
T |
7: 122,999,488 (GRCm38) |
|
probably benign |
Het |
| Rif1 |
GCCACCA |
GCCA |
2: 52,110,324 (GRCm38) |
|
probably benign |
Het |
| Scly |
A |
G |
1: 91,320,169 (GRCm38) |
D413G |
probably damaging |
Het |
| Scn11a |
G |
A |
9: 119,780,795 (GRCm38) |
R996* |
probably null |
Het |
| Slc6a13 |
A |
T |
6: 121,332,373 (GRCm38) |
D281V |
probably damaging |
Het |
| Slfn5 |
T |
C |
11: 82,956,616 (GRCm38) |
V109A |
probably benign |
Het |
| Smyd1 |
A |
T |
6: 71,312,719 (GRCm38) |
|
probably null |
Het |
| Snx29 |
T |
A |
16: 11,367,724 (GRCm38) |
M57K |
probably benign |
Het |
| Stag1 |
T |
G |
9: 100,888,086 (GRCm38) |
I603S |
probably benign |
Het |
| Svep1 |
C |
A |
4: 58,097,292 (GRCm38) |
C1417F |
possibly damaging |
Het |
| Tbc1d23 |
T |
C |
16: 57,189,351 (GRCm38) |
I392V |
probably benign |
Het |
| Tchh |
T |
C |
3: 93,446,799 (GRCm38) |
L1182P |
unknown |
Het |
| Tle3 |
T |
A |
9: 61,394,633 (GRCm38) |
V108E |
probably damaging |
Het |
| Tmem144 |
T |
C |
3: 79,825,400 (GRCm38) |
|
probably null |
Het |
| Tpr |
T |
G |
1: 150,419,907 (GRCm38) |
L894V |
possibly damaging |
Het |
| Trappc9 |
A |
T |
15: 73,000,025 (GRCm38) |
V472E |
probably damaging |
Het |
| Trim38 |
T |
C |
13: 23,791,098 (GRCm38) |
V340A |
probably damaging |
Het |
| Ttc37 |
T |
C |
13: 76,134,815 (GRCm38) |
V752A |
probably benign |
Het |
| Ttn |
A |
T |
2: 76,811,243 (GRCm38) |
L5176Q |
possibly damaging |
Het |
| Vmn1r12 |
T |
A |
6: 57,159,509 (GRCm38) |
I197N |
possibly damaging |
Het |
| Vmn1r26 |
T |
C |
6: 58,009,126 (GRCm38) |
Y26C |
possibly damaging |
Het |
| Vwa3a |
A |
G |
7: 120,758,954 (GRCm38) |
I83V |
probably null |
Het |
| Xirp1 |
C |
T |
9: 120,018,591 (GRCm38) |
E409K |
probably benign |
Het |
| Zfp976 |
A |
G |
7: 42,613,841 (GRCm38) |
C191R |
probably damaging |
Het |
|
| Other mutations in Zc3h14 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00835:Zc3h14
|
APN |
12 |
98,747,524 (GRCm38) |
critical splice donor site |
probably null |
|
|
IGL00946:Zc3h14
|
APN |
12 |
98,759,883 (GRCm38) |
splice site |
probably benign |
|
|
IGL00969:Zc3h14
|
APN |
12 |
98,758,843 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL01626:Zc3h14
|
APN |
12 |
98,779,186 (GRCm38) |
missense |
possibly damaging |
0.72 |
|
IGL01891:Zc3h14
|
APN |
12 |
98,758,947 (GRCm38) |
unclassified |
probably benign |
|
|
IGL02119:Zc3h14
|
APN |
12 |
98,763,895 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL02484:Zc3h14
|
APN |
12 |
98,774,301 (GRCm38) |
missense |
probably benign |
0.14 |
|
IGL02744:Zc3h14
|
APN |
12 |
98,784,975 (GRCm38) |
missense |
possibly damaging |
0.67 |
|
IGL02894:Zc3h14
|
APN |
12 |
98,758,943 (GRCm38) |
critical splice donor site |
probably null |
|
|
R0408:Zc3h14
|
UTSW |
12 |
98,763,823 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0739:Zc3h14
|
UTSW |
12 |
98,757,201 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0865:Zc3h14
|
UTSW |
12 |
98,779,269 (GRCm38) |
critical splice donor site |
probably null |
|
|
R0926:Zc3h14
|
UTSW |
12 |
98,758,590 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R1530:Zc3h14
|
UTSW |
12 |
98,785,003 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1735:Zc3h14
|
UTSW |
12 |
98,758,580 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1743:Zc3h14
|
UTSW |
12 |
98,779,189 (GRCm38) |
missense |
probably benign |
0.04 |
|
R1848:Zc3h14
|
UTSW |
12 |
98,752,832 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R1851:Zc3h14
|
UTSW |
12 |
98,760,354 (GRCm38) |
nonsense |
probably null |
|
|
R2011:Zc3h14
|
UTSW |
12 |
98,780,268 (GRCm38) |
missense |
possibly damaging |
0.76 |
|
R2198:Zc3h14
|
UTSW |
12 |
98,752,810 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R2198:Zc3h14
|
UTSW |
12 |
98,752,809 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2263:Zc3h14
|
UTSW |
12 |
98,758,514 (GRCm38) |
missense |
probably benign |
0.32 |
|
R3762:Zc3h14
|
UTSW |
12 |
98,758,643 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4210:Zc3h14
|
UTSW |
12 |
98,785,399 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4353:Zc3h14
|
UTSW |
12 |
98,763,960 (GRCm38) |
missense |
possibly damaging |
0.70 |
|
R4360:Zc3h14
|
UTSW |
12 |
98,780,197 (GRCm38) |
missense |
probably benign |
0.09 |
|
R4814:Zc3h14
|
UTSW |
12 |
98,752,848 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4815:Zc3h14
|
UTSW |
12 |
98,752,848 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4817:Zc3h14
|
UTSW |
12 |
98,752,848 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4947:Zc3h14
|
UTSW |
12 |
98,759,824 (GRCm38) |
missense |
probably benign |
|
|
R5077:Zc3h14
|
UTSW |
12 |
98,757,206 (GRCm38) |
critical splice donor site |
probably null |
|
|
R5431:Zc3h14
|
UTSW |
12 |
98,780,065 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5783:Zc3h14
|
UTSW |
12 |
98,757,175 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5850:Zc3h14
|
UTSW |
12 |
98,779,155 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R6034:Zc3h14
|
UTSW |
12 |
98,771,373 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6034:Zc3h14
|
UTSW |
12 |
98,771,373 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6291:Zc3h14
|
UTSW |
12 |
98,759,828 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6338:Zc3h14
|
UTSW |
12 |
98,758,590 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6595:Zc3h14
|
UTSW |
12 |
98,757,026 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6737:Zc3h14
|
UTSW |
12 |
98,785,046 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6932:Zc3h14
|
UTSW |
12 |
98,771,077 (GRCm38) |
intron |
probably benign |
|
|
R7074:Zc3h14
|
UTSW |
12 |
98,758,600 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R7204:Zc3h14
|
UTSW |
12 |
98,771,356 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7237:Zc3h14
|
UTSW |
12 |
98,780,149 (GRCm38) |
missense |
probably benign |
0.34 |
|
R7267:Zc3h14
|
UTSW |
12 |
98,785,729 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8753:Zc3h14
|
UTSW |
12 |
98,758,572 (GRCm38) |
missense |
probably benign |
0.12 |
|
R9169:Zc3h14
|
UTSW |
12 |
98,779,246 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9610:Zc3h14
|
UTSW |
12 |
98,771,404 (GRCm38) |
missense |
possibly damaging |
0.92 |
|
RF020:Zc3h14
|
UTSW |
12 |
98,780,282 (GRCm38) |
critical splice donor site |
probably null |
|
|
RF024:Zc3h14
|
UTSW |
12 |
98,758,861 (GRCm38) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CACGTGATATTAGCCGTGGAG -3'
(R):5'- AACAGCAGTTAAAGCATGTGTTGC -3'
Sequencing Primer
(F):5'- CCGTGGAGTTATGAAGTTGTAACC -3'
(R):5'- GCTTAGCCTAGAGAGCATCTC -3'
|
| Posted On |
2014-08-25 |
Incidental Mutation