Mutagenetix > Incidental Mutation

Incidental Mutation 'R2015:Try5'

222778

Institutional Source

Beutler Lab

Gene Symbol

Try5

Ensembl Gene

ENSMUSG00000036938

Gene Name

trypsin 5

Synonyms

1810049H19Rik, Tc

MMRRC Submission

040024-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R2015 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41311233-41316841 bp(-) (GRCm38)

Type of Mutation

splice site (5 bp from exon)

DNA Base Change (assembly)

C to T at 41314651 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000064498 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064324] [ENSMUST00000173916]

AlphaFold

Q9QUK9

Predicted Effect

probably null
Transcript: ENSMUST00000064324

SMART Domains

Protein: ENSMUSP00000064498
Gene: ENSMUSG00000036938

Domain	Start	End	E-Value	Type
low complexity region	4	16	N/A	INTRINSIC
Tryp_SPc	23	239	1.47e-104	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000173916

SMART Domains

Protein: ENSMUSP00000133640
Gene: ENSMUSG00000036938

Domain	Start	End	E-Value	Type
Tryp_SPc	1	131	1.21e-27	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006E09Rik	C	A	11: 101,987,218 (GRCm38)	H35Q	probably damaging	Het
1700015F17Rik	T	A	5: 5,455,964 (GRCm38)	I106L	probably benign	Het
Abca9	A	T	11: 110,131,846 (GRCm38)	M1022K	probably benign	Het
Abhd4	T	A	14: 54,262,832 (GRCm38)	H74Q	probably damaging	Het
Actg1	A	G	11: 120,346,810 (GRCm38)	S49P	possibly damaging	Het
Adcy8	C	T	15: 64,767,878 (GRCm38)	G678S	probably benign	Het
Aire	T	C	10: 78,042,958 (GRCm38)	D85G	probably damaging	Het
Akr1c18	T	A	13: 4,145,309 (GRCm38)	D50V	probably damaging	Het
Ankrd13a	C	A	5: 114,792,109 (GRCm38)	A185E	probably damaging	Het
Apc	A	G	18: 34,315,591 (GRCm38)	I1813V	probably damaging	Het
Armc8	A	T	9: 99,483,105 (GRCm38)	C661*	probably null	Het
Bbs10	C	T	10: 111,300,855 (GRCm38)	Q610*	probably null	Het
Blm	T	C	7: 80,502,399 (GRCm38)	E600G	probably damaging	Het
Bpifb9a	T	C	2: 154,268,200 (GRCm38)		probably null	Het
Cdk14	T	C	5: 5,380,082 (GRCm38)	K15R	probably benign	Het
Cdr1	A	G	X: 61,184,814 (GRCm38)	F249L	probably benign	Het
Cep250	A	C	2: 155,981,453 (GRCm38)	H1009P	probably damaging	Het
Clasp2	A	G	9: 113,911,500 (GRCm38)	T495A	possibly damaging	Het
Col19a1	C	G	1: 24,559,753 (GRCm38)	G53A	unknown	Het
Cpne1	G	A	2: 156,078,388 (GRCm38)	R166C	probably damaging	Het
Dtx3l	G	A	16: 35,936,427 (GRCm38)	H129Y	probably benign	Het
Ercc3	A	G	18: 32,248,429 (GRCm38)	T433A	probably benign	Het
Gm9936	T	A	5: 114,857,421 (GRCm38)		probably benign	Het
Grina	T	C	15: 76,248,534 (GRCm38)	V167A	probably damaging	Het
Hcfc2	A	G	10: 82,738,980 (GRCm38)	N618D	probably benign	Het
Herpud1	T	C	8: 94,392,206 (GRCm38)	V196A	probably benign	Het
Hist3h2a	T	A	11: 58,954,928 (GRCm38)	L64Q	probably damaging	Het
Hlcs	A	G	16: 94,262,740 (GRCm38)	V487A	probably benign	Het
Hspa9	A	T	18: 34,946,648 (GRCm38)	Y243N	probably damaging	Het
Igll1	A	G	16: 16,863,775 (GRCm38)	S39P	probably benign	Het
Kdm5a	T	C	6: 120,431,990 (GRCm38)	S1545P	probably benign	Het
Klra8	C	A	6: 130,115,573 (GRCm38)	C255F	probably damaging	Het
Krtap4-6	A	T	11: 99,665,572 (GRCm38)	C110S	unknown	Het
Lef1	T	A	3: 131,111,587 (GRCm38)	I39N	probably damaging	Het
Lnpep	G	A	17: 17,579,063 (GRCm38)	T110I	probably damaging	Het
Lrp1	T	A	10: 127,540,694 (GRCm38)	T4282S	probably benign	Het
Lrp6	A	G	6: 134,480,374 (GRCm38)		probably null	Het
Ly6g5b	A	C	17: 35,114,678 (GRCm38)	S53A	possibly damaging	Het
M6pr	T	G	6: 122,313,373 (GRCm38)	N98K	probably damaging	Het
Mal2	T	C	15: 54,600,740 (GRCm38)	*176Q	probably null	Het
Mansc1	T	C	6: 134,610,311 (GRCm38)	D301G	possibly damaging	Het
March1	C	A	8: 66,121,821 (GRCm38)	N11K	probably damaging	Het
Mast3	T	A	8: 70,787,363 (GRCm38)	I338F	probably benign	Het
Mcm3	A	G	1: 20,803,580 (GRCm38)	L772P	probably damaging	Het
Mib2	C	T	4: 155,657,880 (GRCm38)	G176D	probably damaging	Het
Mier2	A	T	10: 79,541,202 (GRCm38)		probably null	Het
Mogs	C	T	6: 83,117,650 (GRCm38)	R483*	probably null	Het
Msl2	G	T	9: 101,075,251 (GRCm38)		probably benign	Het
Naa16	A	T	14: 79,345,059 (GRCm38)	M530K	probably damaging	Het
Nde1	A	T	16: 14,169,457 (GRCm38)		probably benign	Het
Ndufb10	T	C	17: 24,722,529 (GRCm38)		probably null	Het
Nhsl1	A	T	10: 18,511,592 (GRCm38)	R205W	probably damaging	Het
Npffr2	T	A	5: 89,582,892 (GRCm38)	I227N	probably damaging	Het
Nup210l	T	A	3: 90,185,432 (GRCm38)	L1231Q	probably damaging	Het
Olfr1355	T	C	10: 78,879,388 (GRCm38)	I72T	possibly damaging	Het
Olfr453	A	G	6: 42,744,850 (GRCm38)	E271G	probably damaging	Het
Pclo	C	A	5: 14,521,501 (GRCm38)	P300Q	probably damaging	Het
Pik3c2a	T	A	7: 116,350,931 (GRCm38)		probably null	Het
Plekha5	T	A	6: 140,534,564 (GRCm38)		probably null	Het
Pls1	A	G	9: 95,761,365 (GRCm38)	V527A	possibly damaging	Het
Ppfia2	T	C	10: 106,474,677 (GRCm38)	M15T	probably benign	Het
Prkd2	T	A	7: 16,847,677 (GRCm38)	C152*	probably null	Het
Ptprq	T	G	10: 107,667,422 (GRCm38)	K792Q	probably damaging	Het
Rbbp8	T	C	18: 11,720,624 (GRCm38)	M296T	probably benign	Het
Rfc3	T	C	5: 151,647,538 (GRCm38)		probably null	Het
Rnf17	A	G	14: 56,486,969 (GRCm38)	N1090S	probably benign	Het
Sash1	A	T	10: 8,729,413 (GRCm38)	V1071D	probably benign	Het
Sdsl	G	A	5: 120,463,153 (GRCm38)	T18M	probably damaging	Het
Sepsecs	T	A	5: 52,647,624 (GRCm38)	Q365L	probably benign	Het
Sgta	A	T	10: 81,051,296 (GRCm38)	V45E	probably damaging	Het
Slc25a46	A	T	18: 31,609,725 (GRCm38)	H29Q	probably benign	Het
Slc2a8	A	C	2: 32,981,380 (GRCm38)	V136G	probably benign	Het
Smg7	A	T	1: 152,860,508 (GRCm38)	N166K	probably damaging	Het
Spata21	C	T	4: 141,107,329 (GRCm38)	Q505*	probably null	Het
Strn4	T	C	7: 16,833,028 (GRCm38)	S458P	possibly damaging	Het
Tbc1d22a	C	T	15: 86,299,684 (GRCm38)	T248M	probably damaging	Het
Trabd	T	A	15: 89,084,726 (GRCm38)	M149K	possibly damaging	Het
Trpv3	A	T	11: 73,279,827 (GRCm38)	N178Y	probably damaging	Het
Tsg101	C	T	7: 46,908,904 (GRCm38)		probably null	Het
Ube3b	A	G	5: 114,411,149 (GRCm38)	E738G	probably damaging	Het
Unc13d	A	G	11: 116,068,755 (GRCm38)	S631P	probably damaging	Het
Unc5d	T	C	8: 28,758,979 (GRCm38)	T297A	probably damaging	Het
Usp18	A	T	6: 121,268,550 (GRCm38)	E1V	probably damaging	Het
Vapb	C	A	2: 173,771,598 (GRCm38)	P97T	probably benign	Het
Vmn1r33	T	A	6: 66,612,372 (GRCm38)	D66V	probably benign	Het
Vmn2r19	T	A	6: 123,315,995 (GRCm38)	M332K	probably benign	Het
Vmn2r71	T	A	7: 85,620,637 (GRCm38)	M452K	probably benign	Het
Vps13b	T	C	15: 35,607,142 (GRCm38)	S1074P	probably damaging	Het
Vwde	C	A	6: 13,208,338 (GRCm38)	G182C	possibly damaging	Het
Wdfy3	A	C	5: 101,860,486 (GRCm38)	S2778A	probably null	Het
Zbtb2	T	A	10: 4,369,757 (GRCm38)	I90F	possibly damaging	Het
Zfp518b	C	A	5: 38,672,002 (GRCm38)	V887F	probably benign	Het
Zfp790	A	G	7: 29,828,861 (GRCm38)	T324A	probably benign	Het
Zufsp	A	G	10: 33,929,824 (GRCm38)	V437A	possibly damaging	Het

Other mutations in Try5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01837:Try5	APN	6	41,313,424 (GRCm38)	missense	probably benign	0.31
IGL01885:Try5	APN	6	41,311,738 (GRCm38)	missense	possibly damaging	0.93
IGL02652:Try5	APN	6	41,311,408 (GRCm38)	missense	probably benign	0.01
R1955:Try5	UTSW	6	41,311,769 (GRCm38)	missense	probably benign	0.17
R2014:Try5	UTSW	6	41,314,651 (GRCm38)	splice site	probably null
R2848:Try5	UTSW	6	41,313,476 (GRCm38)	missense	probably benign	0.01
R4227:Try5	UTSW	6	41,313,467 (GRCm38)	missense	possibly damaging	0.65
R4685:Try5	UTSW	6	41,311,299 (GRCm38)	missense	possibly damaging	0.59
R4816:Try5	UTSW	6	41,313,415 (GRCm38)	missense	probably benign	0.18
R5230:Try5	UTSW	6	41,312,378 (GRCm38)	missense	probably benign	0.19
R5658:Try5	UTSW	6	41,312,427 (GRCm38)	missense	probably damaging	1.00
R6518:Try5	UTSW	6	41,314,679 (GRCm38)	missense	probably benign
R6910:Try5	UTSW	6	41,311,799 (GRCm38)	missense	possibly damaging	0.62
R6913:Try5	UTSW	6	41,311,332 (GRCm38)	missense	probably damaging	1.00
R7219:Try5	UTSW	6	41,311,703 (GRCm38)	missense	probably damaging	1.00
R7242:Try5	UTSW	6	41,313,454 (GRCm38)	missense	probably benign	0.09
R7444:Try5	UTSW	6	41,311,365 (GRCm38)	missense	probably benign	0.00
R7575:Try5	UTSW	6	41,311,814 (GRCm38)	missense	probably benign	0.05
R7585:Try5	UTSW	6	41,311,814 (GRCm38)	missense	probably benign	0.14
R8011:Try5	UTSW	6	41,313,487 (GRCm38)	missense	probably benign	0.12
R8739:Try5	UTSW	6	41,311,703 (GRCm38)	missense	probably damaging	1.00
R8991:Try5	UTSW	6	41,312,361 (GRCm38)	missense	probably benign	0.18
R9397:Try5	UTSW	6	41,312,380 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATTTCCTGGAACTGCCTTATGC -3'
(R):5'- CTGTATGAGGGAAAACCACAGC -3'

Sequencing Primer
(F):5'- GTCTCTCTGACCACATTATACACGAG -3'
(R):5'- GGAAAACCACAGCTGTGTTTGTC -3'

Posted On

2014-08-25