Mutagenetix > Incidental Mutation

Incidental Mutation 'R2015:Apc'

222931

Institutional Source

Beutler Lab

Gene Symbol

Apc

Ensembl Gene

ENSMUSG00000005871

Gene Name

adenomatosis polyposis coli

Synonyms

CC1, Min

MMRRC Submission

040024-MU

Accession Numbers

Ncbi RefSeq: NM_007462.3; MGI:88039

Essential gene?

Probably essential (E-score: 0.971) question?

Stock #

R2015 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

34220924-34322189 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 34315591 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 1813 (I1813V)

Ref Sequence

ENSEMBL: ENSMUSP00000111447 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079362] [ENSMUST00000115781] [ENSMUST00000171187]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000079362
AA Change: I1847V

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000078337
Gene: ENSMUSG00000005871
AA Change: I1847V

Domain	Start	End	E-Value	Type
Pfam:APC_N_CC	4	55	6e-32	PFAM
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	125	205	2e-24	PFAM
low complexity region	211	222	N/A	INTRINSIC
low complexity region	234	247	N/A	INTRINSIC
ARM	338	390	6.14e-5	SMART
ARM	457	508	1.62e-4	SMART
ARM	510	551	8.56e-4	SMART
ARM	554	595	4.45e-2	SMART
ARM	597	642	5.76e1	SMART
ARM	647	687	1.29e-7	SMART
Pfam:Arm_APC_u3	730	1017	5e-170	PFAM
Pfam:APC_15aa	1018	1032	1.1e-8	PFAM
Pfam:APC_u5	1034	1133	7.6e-55	PFAM
Pfam:APC_15aa	1154	1168	1.6e-8	PFAM
Pfam:APC_15aa	1171	1185	1.9e-9	PFAM
low complexity region	1187	1204	N/A	INTRINSIC
Pfam:APC_crr	1255	1279	1.5e-15	PFAM
Pfam:APC_u9	1280	1367	1.9e-34	PFAM
Pfam:APC_crr	1370	1393	2.2e-10	PFAM
low complexity region	1431	1449	N/A	INTRINSIC
Pfam:APC_crr	1485	1509	2.1e-9	PFAM
low complexity region	1532	1548	N/A	INTRINSIC
Pfam:SAMP	1568	1587	2.7e-11	PFAM
Pfam:APC_crr	1635	1659	1.9e-15	PFAM
Pfam:APC_u13	1660	1716	1.3e-31	PFAM
Pfam:SAMP	1717	1736	3.2e-12	PFAM
Pfam:APC_u14	1737	1837	1e-46	PFAM
Pfam:APC_crr	1839	1864	6.8e-15	PFAM
Pfam:APC_u15	1865	1945	1.8e-40	PFAM
Pfam:APC_crr	1947	1971	1.6e-14	PFAM
Pfam:APC_crr	2007	2030	1.8e-14	PFAM
Pfam:SAMP	2033	2052	1.6e-13	PFAM
low complexity region	2112	2146	N/A	INTRINSIC
Pfam:APC_basic	2223	2579	1.5e-110	PFAM
low complexity region	2626	2638	N/A	INTRINSIC
Pfam:EB1_binding	2670	2842	9.3e-89	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000115781
AA Change: I1813V

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000111447
Gene: ENSMUSG00000005871
AA Change: I1813V

Domain	Start	End	E-Value	Type
PDB:1DEB\|B	2	55	1e-27	PDB
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	124	206	1.5e-31	PFAM
low complexity region	211	222	N/A	INTRINSIC
ARM	304	356	6.14e-5	SMART
ARM	423	474	1.62e-4	SMART
ARM	476	517	8.56e-4	SMART
ARM	520	561	4.45e-2	SMART
ARM	563	608	5.76e1	SMART
ARM	613	653	1.29e-7	SMART
Pfam:Arm	655	695	1.7e-6	PFAM
low complexity region	797	810	N/A	INTRINSIC
low complexity region	880	892	N/A	INTRINSIC
low complexity region	923	935	N/A	INTRINSIC
Pfam:APC_15aa	984	999	3.7e-9	PFAM
Pfam:APC_15aa	1100	1115	8.4e-8	PFAM
Pfam:APC_15aa	1120	1135	9.9e-9	PFAM
Pfam:APC_15aa	1137	1152	1.2e-9	PFAM
low complexity region	1153	1170	N/A	INTRINSIC
Pfam:APC_crr	1220	1245	7.5e-15	PFAM
low complexity region	1320	1331	N/A	INTRINSIC
Pfam:APC_crr	1334	1359	2.8e-11	PFAM
low complexity region	1397	1415	N/A	INTRINSIC
Pfam:APC_crr	1450	1475	2.2e-8	PFAM
low complexity region	1498	1514	N/A	INTRINSIC
Pfam:SAMP	1533	1553	8.4e-12	PFAM
Pfam:APC_crr	1600	1625	3.5e-13	PFAM
Pfam:SAMP	1682	1702	5e-12	PFAM
low complexity region	1732	1744	N/A	INTRINSIC
Pfam:APC_crr	1805	1830	3.1e-12	PFAM
low complexity region	1866	1877	N/A	INTRINSIC
Pfam:APC_crr	1912	1937	3.5e-13	PFAM
Pfam:APC_crr	1971	1996	7.1e-14	PFAM
Pfam:SAMP	1999	2018	4.6e-13	PFAM
low complexity region	2078	2112	N/A	INTRINSIC
Pfam:APC_basic	2189	2545	1.1e-131	PFAM
low complexity region	2592	2604	N/A	INTRINSIC
Pfam:EB1_binding	2636	2808	2.9e-90	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000165590

SMART Domains

Protein: ENSMUSP00000128327
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
PDB:3AU3\|A	2	33	2e-17	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167136

Predicted Effect

probably benign
Transcript: ENSMUST00000171187

SMART Domains

Protein: ENSMUSP00000127131
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
low complexity region	9	20	N/A	INTRINSIC
low complexity region	23	52	N/A	INTRINSIC
low complexity region	102	119	N/A	INTRINSIC
Pfam:Suppressor_APC	134	216	5.2e-32	PFAM
ARM	320	372	6.14e-5	SMART
ARM	439	490	1.62e-4	SMART
ARM	492	533	8.56e-4	SMART
ARM	536	577	4.45e-2	SMART
ARM	579	624	5.76e1	SMART
ARM	629	669	1.29e-7	SMART
Pfam:Arm	671	711	6.3e-7	PFAM
low complexity region	813	826	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	939	951	N/A	INTRINSIC
Pfam:APC_15aa	1000	1015	1.4e-9	PFAM
Pfam:APC_15aa	1116	1131	3.1e-8	PFAM

Meta Mutation Damage Score

0.0951

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

MGI Phenotype

Strain: 1856318; 2387050; 1857951; 1857957
Lethality: E8-E12
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
PHENOTYPE: Most targeted and hypomorphic heterozygous mutants develop intestinal polyps and colorectal cancer, associated with anemia from intestinal bleeding. Homozygotes are embryonic lethal. Homozygotes for a mild alleles survive and have less extreme tumor incidence. [provided by MGI curators]

Allele List at MGI

All alleles(88) : Targeted(25) Gene trapped(62) Chemically induced(1)

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006E09Rik	C	A	11: 101,987,218	H35Q	probably damaging	Het
1700015F17Rik	T	A	5: 5,455,964	I106L	probably benign	Het
Abca9	A	T	11: 110,131,846	M1022K	probably benign	Het
Abhd4	T	A	14: 54,262,832	H74Q	probably damaging	Het
Actg1	A	G	11: 120,346,810	S49P	possibly damaging	Het
Adcy8	C	T	15: 64,767,878	G678S	probably benign	Het
Aire	T	C	10: 78,042,958	D85G	probably damaging	Het
Akr1c18	T	A	13: 4,145,309	D50V	probably damaging	Het
Ankrd13a	C	A	5: 114,792,109	A185E	probably damaging	Het
Armc8	A	T	9: 99,483,105	C661*	probably null	Het
Bbs10	C	T	10: 111,300,855	Q610*	probably null	Het
Blm	T	C	7: 80,502,399	E600G	probably damaging	Het
Bpifb9a	T	C	2: 154,268,200		probably null	Het
Cdk14	T	C	5: 5,380,082	K15R	probably benign	Het
Cdr1	A	G	X: 61,184,814	F249L	probably benign	Het
Cep250	A	C	2: 155,981,453	H1009P	probably damaging	Het
Clasp2	A	G	9: 113,911,500	T495A	possibly damaging	Het
Col19a1	C	G	1: 24,559,753	G53A	unknown	Het
Cpne1	G	A	2: 156,078,388	R166C	probably damaging	Het
Dtx3l	G	A	16: 35,936,427	H129Y	probably benign	Het
Ercc3	A	G	18: 32,248,429	T433A	probably benign	Het
Gm9936	T	A	5: 114,857,421		probably benign	Het
Grina	T	C	15: 76,248,534	V167A	probably damaging	Het
Hcfc2	A	G	10: 82,738,980	N618D	probably benign	Het
Herpud1	T	C	8: 94,392,206	V196A	probably benign	Het
Hist3h2a	T	A	11: 58,954,928	L64Q	probably damaging	Het
Hlcs	A	G	16: 94,262,740	V487A	probably benign	Het
Hspa9	A	T	18: 34,946,648	Y243N	probably damaging	Het
Igll1	A	G	16: 16,863,775	S39P	probably benign	Het
Kdm5a	T	C	6: 120,431,990	S1545P	probably benign	Het
Klra8	C	A	6: 130,115,573	C255F	probably damaging	Het
Krtap4-6	A	T	11: 99,665,572	C110S	unknown	Het
Lef1	T	A	3: 131,111,587	I39N	probably damaging	Het
Lnpep	G	A	17: 17,579,063	T110I	probably damaging	Het
Lrp1	T	A	10: 127,540,694	T4282S	probably benign	Het
Lrp6	A	G	6: 134,480,374		probably null	Het
Ly6g5b	A	C	17: 35,114,678	S53A	possibly damaging	Het
M6pr	T	G	6: 122,313,373	N98K	probably damaging	Het
Mal2	T	C	15: 54,600,740	*176Q	probably null	Het
Mansc1	T	C	6: 134,610,311	D301G	possibly damaging	Het
March1	C	A	8: 66,121,821	N11K	probably damaging	Het
Mast3	T	A	8: 70,787,363	I338F	probably benign	Het
Mcm3	A	G	1: 20,803,580	L772P	probably damaging	Het
Mib2	C	T	4: 155,657,880	G176D	probably damaging	Het
Mier2	A	T	10: 79,541,202		probably null	Het
Mogs	C	T	6: 83,117,650	R483*	probably null	Het
Msl2	G	T	9: 101,075,251		probably benign	Het
Naa16	A	T	14: 79,345,059	M530K	probably damaging	Het
Nde1	A	T	16: 14,169,457		probably benign	Het
Ndufb10	T	C	17: 24,722,529		probably null	Het
Nhsl1	A	T	10: 18,511,592	R205W	probably damaging	Het
Npffr2	T	A	5: 89,582,892	I227N	probably damaging	Het
Nup210l	T	A	3: 90,185,432	L1231Q	probably damaging	Het
Olfr1355	T	C	10: 78,879,388	I72T	possibly damaging	Het
Olfr453	A	G	6: 42,744,850	E271G	probably damaging	Het
Pclo	C	A	5: 14,521,501	P300Q	probably damaging	Het
Pik3c2a	T	A	7: 116,350,931		probably null	Het
Plekha5	T	A	6: 140,534,564		probably null	Het
Pls1	A	G	9: 95,761,365	V527A	possibly damaging	Het
Ppfia2	T	C	10: 106,474,677	M15T	probably benign	Het
Prkd2	T	A	7: 16,847,677	C152*	probably null	Het
Ptprq	T	G	10: 107,667,422	K792Q	probably damaging	Het
Rbbp8	T	C	18: 11,720,624	M296T	probably benign	Het
Rfc3	T	C	5: 151,647,538		probably null	Het
Rnf17	A	G	14: 56,486,969	N1090S	probably benign	Het
Sash1	A	T	10: 8,729,413	V1071D	probably benign	Het
Sdsl	G	A	5: 120,463,153	T18M	probably damaging	Het
Sepsecs	T	A	5: 52,647,624	Q365L	probably benign	Het
Sgta	A	T	10: 81,051,296	V45E	probably damaging	Het
Slc25a46	A	T	18: 31,609,725	H29Q	probably benign	Het
Slc2a8	A	C	2: 32,981,380	V136G	probably benign	Het
Smg7	A	T	1: 152,860,508	N166K	probably damaging	Het
Spata21	C	T	4: 141,107,329	Q505*	probably null	Het
Strn4	T	C	7: 16,833,028	S458P	possibly damaging	Het
Tbc1d22a	C	T	15: 86,299,684	T248M	probably damaging	Het
Trabd	T	A	15: 89,084,726	M149K	possibly damaging	Het
Trpv3	A	T	11: 73,279,827	N178Y	probably damaging	Het
Try5	C	T	6: 41,314,651		probably null	Het
Tsg101	C	T	7: 46,908,904		probably null	Het
Ube3b	A	G	5: 114,411,149	E738G	probably damaging	Het
Unc13d	A	G	11: 116,068,755	S631P	probably damaging	Het
Unc5d	T	C	8: 28,758,979	T297A	probably damaging	Het
Usp18	A	T	6: 121,268,550	E1V	probably damaging	Het
Vapb	C	A	2: 173,771,598	P97T	probably benign	Het
Vmn1r33	T	A	6: 66,612,372	D66V	probably benign	Het
Vmn2r19	T	A	6: 123,315,995	M332K	probably benign	Het
Vmn2r71	T	A	7: 85,620,637	M452K	probably benign	Het
Vps13b	T	C	15: 35,607,142	S1074P	probably damaging	Het
Vwde	C	A	6: 13,208,338	G182C	possibly damaging	Het
Wdfy3	A	C	5: 101,860,486	S2778A	probably null	Het
Zbtb2	T	A	10: 4,369,757	I90F	possibly damaging	Het
Zfp518b	C	A	5: 38,672,002	V887F	probably benign	Het
Zfp790	A	G	7: 29,828,861	T324A	probably benign	Het
Zufsp	A	G	10: 33,929,824	V437A	possibly damaging	Het

Other mutations in Apc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00507:Apc	APN	18	34316926	missense	probably benign	0.01
IGL00898:Apc	APN	18	34317094	missense	probably damaging	1.00
IGL01111:Apc	APN	18	34315136	missense	possibly damaging	0.95
IGL01347:Apc	APN	18	34317670	missense	probably damaging	1.00
IGL01375:Apc	APN	18	34313654	missense	probably damaging	1.00
IGL01805:Apc	APN	18	34318218	missense	probably benign	0.02
IGL01997:Apc	APN	18	34315423	missense	probably benign	0.00
IGL02033:Apc	APN	18	34310719	missense	probably damaging	1.00
IGL02323:Apc	APN	18	34315810	nonsense	probably null
IGL02373:Apc	APN	18	34316159	missense	probably damaging	1.00
IGL02379:Apc	APN	18	34298745	missense	probably benign	0.45
IGL02456:Apc	APN	18	34313882	nonsense	probably null
IGL02552:Apc	APN	18	34312982	missense	possibly damaging	0.90
IGL02676:Apc	APN	18	34315634	missense	probably damaging	1.00
IGL02756:Apc	APN	18	34314535	missense	probably damaging	1.00
IGL02938:Apc	APN	18	34315228	missense	probably damaging	0.98
IGL02974:Apc	APN	18	34268383	splice site	probably benign
IGL03124:Apc	APN	18	34299985	missense	probably damaging	0.98
IGL03201:Apc	APN	18	34312376	missense	probably damaging	1.00
IGL03339:Apc	APN	18	34298474	missense	probably damaging	1.00
FR4304:Apc	UTSW	18	34281997	intron	probably benign
FR4342:Apc	UTSW	18	34281999	intron	probably benign
FR4449:Apc	UTSW	18	34282000	intron	probably benign
FR4449:Apc	UTSW	18	34282005	intron	probably benign
FR4548:Apc	UTSW	18	34281998	intron	probably benign
FR4737:Apc	UTSW	18	34281999	intron	probably benign
FR4976:Apc	UTSW	18	34281998	intron	probably benign
FR4976:Apc	UTSW	18	34282000	intron	probably benign
FR4976:Apc	UTSW	18	34282004	nonsense	probably null
R0385:Apc	UTSW	18	34315944	missense	probably damaging	1.00
R0535:Apc	UTSW	18	34261072	missense	probably damaging	1.00
R0561:Apc	UTSW	18	34313303	missense	possibly damaging	0.94
R0590:Apc	UTSW	18	34316230	nonsense	probably null
R0626:Apc	UTSW	18	34318454	missense	probably damaging	1.00
R0991:Apc	UTSW	18	34316107	missense	probably damaging	1.00
R1564:Apc	UTSW	18	34315149	missense	probably benign	0.00
R1663:Apc	UTSW	18	34268325	missense	probably damaging	0.98
R1737:Apc	UTSW	18	34317022	missense	probably damaging	1.00
R1739:Apc	UTSW	18	34312318	missense	probably damaging	1.00
R1835:Apc	UTSW	18	34317077	missense	probably damaging	1.00
R1887:Apc	UTSW	18	34272468	missense	probably damaging	1.00
R1957:Apc	UTSW	18	34317335	missense	probably damaging	1.00
R1974:Apc	UTSW	18	34300004	missense	possibly damaging	0.62
R2005:Apc	UTSW	18	34310909	critical splice donor site	probably null
R2013:Apc	UTSW	18	34315591	missense	probably damaging	0.98
R2014:Apc	UTSW	18	34315591	missense	probably damaging	0.98
R2017:Apc	UTSW	18	34313602	missense	probably benign	0.00
R2056:Apc	UTSW	18	34316428	missense	probably damaging	1.00
R2108:Apc	UTSW	18	34269229	missense	probably damaging	1.00
R2120:Apc	UTSW	18	34276601	missense	probably damaging	1.00
R2131:Apc	UTSW	18	34312045	missense	possibly damaging	0.51
R2133:Apc	UTSW	18	34312045	missense	possibly damaging	0.51
R2291:Apc	UTSW	18	34312491	missense	probably benign	0.45
R2332:Apc	UTSW	18	34317059	missense	possibly damaging	0.50
R2360:Apc	UTSW	18	34261126	missense	probably damaging	1.00
R2407:Apc	UTSW	18	34314262	missense	possibly damaging	0.77
R2507:Apc	UTSW	18	34316537	missense	possibly damaging	0.77
R2940:Apc	UTSW	18	34276670	missense	probably damaging	1.00
R3404:Apc	UTSW	18	34313602	missense	probably benign	0.00
R3411:Apc	UTSW	18	34269259	splice site	probably benign
R3778:Apc	UTSW	18	34313081	missense	probably damaging	1.00
R3826:Apc	UTSW	18	34279335	missense	possibly damaging	0.93
R4599:Apc	UTSW	18	34317987	nonsense	probably null
R4611:Apc	UTSW	18	34318565	missense	probably damaging	1.00
R4664:Apc	UTSW	18	34298594	missense	probably damaging	0.98
R4969:Apc	UTSW	18	34312918	nonsense	probably null
R5007:Apc	UTSW	18	34312963	missense	probably damaging	1.00
R5066:Apc	UTSW	18	34316105	missense	probably damaging	1.00
R5112:Apc	UTSW	18	34316109	nonsense	probably null
R5259:Apc	UTSW	18	34314290	missense	probably benign	0.29
R5440:Apc	UTSW	18	34221160	unclassified	probably benign
R5508:Apc	UTSW	18	34298580	missense	probably damaging	0.97
R5512:Apc	UTSW	18	34310909	critical splice donor site	probably benign
R5850:Apc	UTSW	18	34318063	missense	possibly damaging	0.94
R5951:Apc	UTSW	18	34317146	missense	possibly damaging	0.89
R5966:Apc	UTSW	18	34221087	utr 5 prime	probably benign
R6081:Apc	UTSW	18	34290111	missense	possibly damaging	0.93
R6116:Apc	UTSW	18	34316455	missense	probably damaging	1.00
R6351:Apc	UTSW	18	34312212	missense	probably damaging	1.00
R6354:Apc	UTSW	18	34312528	missense	probably benign	0.02
R6467:Apc	UTSW	18	34269199	missense	probably benign	0.22
R6974:Apc	UTSW	18	34298427	missense	possibly damaging	0.65
R7027:Apc	UTSW	18	34312076	missense	probably damaging	1.00
R7096:Apc	UTSW	18	34315957	missense	probably damaging	1.00
R7289:Apc	UTSW	18	34315271	missense	probably damaging	1.00
R7439:Apc	UTSW	18	34312073	missense	probably damaging	1.00
R7441:Apc	UTSW	18	34312073	missense	probably damaging	1.00
R7534:Apc	UTSW	18	34316962	missense	probably damaging	1.00
R7685:Apc	UTSW	18	34314208	missense	probably damaging	1.00
R7814:Apc	UTSW	18	34272539	missense	probably damaging	0.98
R7954:Apc	UTSW	18	34314268	missense	probably damaging	0.99
R8352:Apc	UTSW	18	34312751	missense	possibly damaging	0.54
R8452:Apc	UTSW	18	34312751	missense	possibly damaging	0.54
R8497:Apc	UTSW	18	34313030	missense	possibly damaging	0.81
R8545:Apc	UTSW	18	34317031	missense	possibly damaging	0.94
R8554:Apc	UTSW	18	34312946	missense	probably damaging	1.00
R8955:Apc	UTSW	18	34268317	missense	probably damaging	1.00
R9014:Apc	UTSW	18	34221021	start gained	probably benign
R9061:Apc	UTSW	18	34313198	missense	probably damaging	1.00
R9147:Apc	UTSW	18	34317657	missense	probably damaging	1.00
R9318:Apc	UTSW	18	34313987	missense	possibly damaging	0.69
R9521:Apc	UTSW	18	34312685	missense	probably benign	0.24
R9546:Apc	UTSW	18	34312258	missense	possibly damaging	0.86
R9547:Apc	UTSW	18	34312258	missense	possibly damaging	0.86
R9557:Apc	UTSW	18	34318359	missense	probably damaging	1.00
R9592:Apc	UTSW	18	34310770	nonsense	probably null
R9675:Apc	UTSW	18	34316194	missense	probably damaging	1.00
R9736:Apc	UTSW	18	34317770	missense	probably damaging	1.00
R9792:Apc	UTSW	18	34314575	missense	probably damaging	1.00
R9793:Apc	UTSW	18	34314575	missense	probably damaging	1.00
R9795:Apc	UTSW	18	34314575	missense	probably damaging	1.00
RF046:Apc	UTSW	18	34282009	critical splice donor site	probably benign
RF063:Apc	UTSW	18	34282009	critical splice donor site	probably benign
X0021:Apc	UTSW	18	34312108	missense	probably damaging	1.00
X0025:Apc	UTSW	18	34312376	missense	probably damaging	1.00
Z1088:Apc	UTSW	18	34313167	nonsense	probably null
Z1177:Apc	UTSW	18	34314463	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- TTCACCAGTAAAGCCCATGC -3'
(R):5'- CTTGTGACTTACTAGCTGCCTG -3'

Sequencing Primer
(F):5'- TACTGAATATAGAACGCGTGTGAG -3'
(R):5'- CCTGCTGGCTTGAGTTTGGTTC -3'

Posted On

2014-08-25