Mutagenetix > Incidental Mutation

Incidental Mutation 'R1992:Kalrn'

223744

Institutional Source

Beutler Lab

Gene Symbol

Kalrn

Ensembl Gene

ENSMUSG00000061751

Gene Name

kalirin, RhoGEF kinase

Synonyms

2210407G14Rik, Hapip, E530005C20Rik, LOC224126

MMRRC Submission

040003-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.934) question?

Stock #

R1992 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

33969073-34573532 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 33975738 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 1222 (L1222F)

Ref Sequence

ENSEMBL: ENSMUSP00000110624 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076810] [ENSMUST00000114973]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000076810
AA Change: L2891F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000076088
Gene: ENSMUSG00000061751
AA Change: L2891F

Domain	Start	End	E-Value	Type
SEC14	20	159	2.22e-30	SMART
SPEC	173	289	5.32e-9	SMART
SPEC	295	397	1.19e-11	SMART
SPEC	400	515	1.83e0	SMART
SPEC	521	623	9.84e-13	SMART
SPEC	626	748	2.74e-2	SMART
SPEC	875	976	8.11e-14	SMART
SPEC	1106	1208	4.7e-10	SMART
RhoGEF	1258	1428	3.6e-56	SMART
PH	1442	1555	5.24e-8	SMART
SH3	1622	1683	1.23e-7	SMART
RhoGEF	1904	2074	1.47e-52	SMART
PH	2094	2199	9.87e-4	SMART
SH3	2295	2356	2.78e-2	SMART
IGc2	2455	2527	4.28e-12	SMART
FN3	2541	2623	3.07e-11	SMART
S_TKc	2656	2910	1.28e-71	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000114973
AA Change: L1222F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110624
Gene: ENSMUSG00000061751
AA Change: L1222F

Domain	Start	End	E-Value	Type
RhoGEF	235	405	1.47e-52	SMART
PH	425	530	9.87e-4	SMART
SH3	626	687	2.78e-2	SMART
IGc2	786	858	4.28e-12	SMART
FN3	872	954	3.07e-11	SMART
S_TKc	987	1241	1.28e-71	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132002

Meta Mutation Damage Score

0.1407

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. [provided by RefSeq, Apr 2016]
PHENOTYPE: Mice homozygous for a knock-out allele specific for isoform 7 exhibit decreased anxiety-related behavior, contextual conditioning, and synapse formation. Mice homozygous for another knock-out allele exhibit impaired AMPA-mediated synaptic currents and abnormal behavior. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 123 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	A	G	12: 55,338,206	D89G	probably damaging	Het
1600015I10Rik	T	A	6: 48,930,769	H234Q	probably damaging	Het
2310009B15Rik	T	C	1: 138,853,642	T90A	probably damaging	Het
4430402I18Rik	A	G	19: 28,948,624	F130L	probably damaging	Het
4932438A13Rik	A	T	3: 37,000,032	H3100L	probably benign	Het
A930011G23Rik	T	C	5: 99,233,925	D326G	possibly damaging	Het
Abcc2	A	T	19: 43,807,142	I446F	probably damaging	Het
Adamts14	A	G	10: 61,198,660	Y1150H	probably benign	Het
Adgrl2	A	G	3: 148,817,244	I217T	possibly damaging	Het
Afap1l1	A	T	18: 61,741,771	Y446*	probably null	Het
Aimp2	G	A	5: 143,906,730	A14V	probably damaging	Het
Akap8	G	A	17: 32,316,612	H143Y	probably damaging	Het
Aldh2	A	T	5: 121,575,963	V207E	possibly damaging	Het
Armc3	T	C	2: 19,293,142	Y575H	probably damaging	Het
Arpp21	T	C	9: 112,157,793	E230G	probably damaging	Het
Arrdc3	G	A	13: 80,883,689	D14N	probably damaging	Het
Atn1	A	G	6: 124,745,328		probably benign	Het
Atxn7l1	G	A	12: 33,358,744	D302N	probably damaging	Het
Bbs1	T	A	19: 4,891,708	H518L	probably benign	Het
Bmpr1a	C	A	14: 34,425,093	G241C	probably damaging	Het
Cacna1b	G	A	2: 24,732,306	P222L	probably damaging	Het
Calcrl	T	C	2: 84,370,511	Y63C	probably damaging	Het
Cand2	A	G	6: 115,785,132	H173R	possibly damaging	Het
Cap2	T	A	13: 46,637,881	Y175N	possibly damaging	Het
Card14	A	T	11: 119,321,821		probably null	Het
Cd3d	T	A	9: 44,985,001	Y29*	probably null	Het
Cd4	A	G	6: 124,867,688	V378A	possibly damaging	Het
Cluh	A	T	11: 74,660,002	H318L	probably damaging	Het
Clvs1	T	A	4: 9,281,899	D114E	probably benign	Het
Cnot4	T	A	6: 35,023,409	T548S	probably benign	Het
Col18a1	T	C	10: 77,081,154	I114V	unknown	Het
Cr2	G	A	1: 195,154,150	P1278S	possibly damaging	Het
Crebbp	A	T	16: 4,128,697		probably null	Het
D430042O09Rik	A	G	7: 125,820,089	N476S	probably benign	Het
Dnah7a	T	G	1: 53,582,676	K1097Q	possibly damaging	Het
Doc2a	T	A	7: 126,851,807		probably null	Het
Dpp10	A	T	1: 123,905,106	V48E	probably null	Het
Dsg2	A	G	18: 20,601,473	K836R	probably damaging	Het
Egr2	T	C	10: 67,540,027	V164A	probably damaging	Het
Erbin	A	T	13: 103,833,713	S1132T	probably benign	Het
Espn	T	A	4: 152,128,555		probably null	Het
Fam83b	A	G	9: 76,492,022	S600P	probably benign	Het
Fanca	A	T	8: 123,297,812	N425K	possibly damaging	Het
Flot2	C	T	11: 78,058,619	L294F	probably damaging	Het
Frs2	A	G	10: 117,074,554	V301A	probably benign	Het
Fv1	T	A	4: 147,869,161	N61K	possibly damaging	Het
Fxr2	A	G	11: 69,649,833	E339G	possibly damaging	Het
Gck	T	C	11: 5,906,515	Y214C	probably damaging	Het
Gm4858	G	T	3: 93,074,037	A121S	probably benign	Het
Gm5346	T	C	8: 43,627,139	K16R	probably benign	Het
Gm9573	A	T	17: 35,618,708	S1529T	probably benign	Het
Golga3	C	G	5: 110,192,973	T551R	probably damaging	Het
Gorab	A	G	1: 163,397,056	S59P	probably damaging	Het
Gsdme	C	T	6: 50,208,122	V451M	probably damaging	Het
H2-Bl	A	C	17: 36,081,046	I216S	probably damaging	Het
Herc4	T	A	10: 63,245,964	V22E	possibly damaging	Het
Hjurp	GT	GTT	1: 88,266,524		probably null	Het
Hoxa2	A	T	6: 52,164,596	S17T	probably damaging	Het
Hs3st5	A	G	10: 36,832,886	Y139C	probably damaging	Het
Ism1	G	A	2: 139,746,017	V221I	probably benign	Het
Kdm4c	A	G	4: 74,343,394	D602G	possibly damaging	Het
Ktn1	A	G	14: 47,695,521	K711E	probably damaging	Het
Lce1k	A	T	3: 92,806,818	C20S	unknown	Het
Macf1	A	G	4: 123,456,695	S3792P	probably damaging	Het
Maml3	C	T	3: 51,690,757	M189I	probably benign	Het
Mcc	C	A	18: 44,491,315	E213*	probably null	Het
Mep1a	T	G	17: 43,502,682	I13L	probably benign	Het
Mkrn2	G	T	6: 115,609,601	C16F	probably damaging	Het
Mlh1	C	T	9: 111,228,563	A727T	probably damaging	Het
Naa16	T	C	14: 79,356,491	Y344C	probably damaging	Het
Nebl	T	A	2: 17,452,510	I80F	probably damaging	Het
Nlrc4	T	A	17: 74,445,633	Y585F	probably benign	Het
Nrcam	A	G	12: 44,540,970	K149R	probably damaging	Het
Nufip1	T	A	14: 76,134,847	I467N	probably damaging	Het
Nup133	A	G	8: 123,906,221	I1057T	possibly damaging	Het
Obscn	A	T	11: 58,995,827		probably benign	Het
Olfr1113	T	A	2: 87,213,244	C117*	probably null	Het
Olfr173	A	G	16: 58,796,946	V300A	probably benign	Het
Olfr177	A	C	16: 58,872,511	I213R	probably benign	Het
Pi4k2a	A	T	19: 42,115,938	I380F	probably damaging	Het
Piezo2	A	T	18: 63,074,662	L1426Q	probably null	Het
Pik3cg	A	T	12: 32,204,025	D654E	possibly damaging	Het
Poli	G	T	18: 70,508,987	P714Q	probably damaging	Het
Polr3f	A	G	2: 144,536,310	N201D	probably benign	Het
Ptprz1	A	T	6: 22,959,748	K81N	probably benign	Het
Rbm19	T	C	5: 120,133,883		probably null	Het
Sec24a	C	T	11: 51,736,363	V241I	probably benign	Het
Sgk3	A	G	1: 9,880,342	T160A	possibly damaging	Het
Slc1a4	A	G	11: 20,304,375	I497T	probably benign	Het
Slc26a9	A	T	1: 131,762,794	D512V	probably damaging	Het
Slc43a3	C	T	2: 84,957,740	R489C	probably damaging	Het
Slc5a12	T	A	2: 110,621,744	F327L	probably benign	Het
Slitrk3	A	G	3: 73,049,771	V556A	possibly damaging	Het
Spib	T	C	7: 44,528,857	E180G	probably benign	Het
Spint2	T	A	7: 29,259,408	N128Y	probably damaging	Het
Spx	G	A	6: 142,418,519	G102E	probably benign	Het
Spz1	T	A	13: 92,575,658	E103D	possibly damaging	Het
Sstr2	T	C	11: 113,624,669	I138T	probably benign	Het
Sytl3	T	A	17: 6,733,049	I206N	possibly damaging	Het
Tcaf2	T	C	6: 42,629,857	T388A	probably benign	Het
Thap12	T	C	7: 98,716,365	V580A	possibly damaging	Het
Tlr5	T	A	1: 182,974,347	D405E	probably damaging	Het
Tnxb	T	C	17: 34,671,904	V407A	probably damaging	Het
Tpx2	T	A	2: 152,890,624	M606K	probably benign	Het
Trim43a	T	G	9: 88,584,259	L211R	probably damaging	Het
Trim46	A	T	3: 89,237,701	Y489N	probably damaging	Het
Ttc28	C	T	5: 111,276,322	S1485L	probably benign	Het
Ttll8	T	C	15: 88,914,451	T694A	probably benign	Het
Txnl1	T	C	18: 63,679,514	T70A	probably benign	Het
Ube3a	C	T	7: 59,303,787	A823V	probably damaging	Het
Ubqln5	C	A	7: 104,129,534	V28F	probably damaging	Het
Ulk1	T	A	5: 110,787,151	Q972L	probably damaging	Het
Unc93b1	T	C	19: 3,944,062	Y398H	probably benign	Het
Usp1	A	G	4: 98,934,294	D615G	probably benign	Het
Utp15	A	G	13: 98,250,912	C385R	probably benign	Het
Vmn2r91	T	A	17: 18,135,880	V603D	probably damaging	Het
Whamm	C	T	7: 81,591,771	R277*	probably null	Het
Wnt8a	A	G	18: 34,544,884	D115G	probably damaging	Het
Yjefn3	A	T	8: 69,888,995		probably null	Het
Ywhab	A	T	2: 164,011,887	I95F	probably damaging	Het
Zfp143	C	T	7: 110,061,282		probably benign	Het
Zfp607b	C	G	7: 27,702,524	T135R	possibly damaging	Het
Zfp970	A	G	2: 177,474,870	Q79R	possibly damaging	Het

Other mutations in Kalrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01361:Kalrn	APN	16	34175722	splice site	probably benign
IGL01364:Kalrn	APN	16	34262629	missense	probably damaging	1.00
IGL01510:Kalrn	APN	16	34235330	missense	possibly damaging	0.52
IGL01664:Kalrn	APN	16	34294161	missense	probably damaging	1.00
IGL01934:Kalrn	APN	16	34198512	splice site	probably null
IGL02059:Kalrn	APN	16	34252341	missense	possibly damaging	0.95
IGL02102:Kalrn	APN	16	34220222	missense	probably damaging	1.00
IGL02306:Kalrn	APN	16	34310527	missense	probably damaging	0.97
IGL02328:Kalrn	APN	16	34332224	missense	probably damaging	0.98
IGL02532:Kalrn	APN	16	34360846	missense	probably damaging	1.00
IGL02685:Kalrn	APN	16	34513959	nonsense	probably null
IGL02696:Kalrn	APN	16	34220114	missense	probably damaging	1.00
IGL02708:Kalrn	APN	16	34392050	missense	probably damaging	1.00
IGL02937:Kalrn	APN	16	34220130	nonsense	probably null
IGL03188:Kalrn	APN	16	34314192	missense	probably benign	0.01
IGL03289:Kalrn	APN	16	34385297	missense	possibly damaging	0.90
IGL03408:Kalrn	APN	16	34314176	missense	probably damaging	0.99
breeze	UTSW	16	34013675	missense
ethereal	UTSW	16	33975435	utr 3 prime	probably benign
Feather	UTSW	16	34314209	missense	probably damaging	0.99
Hidden	UTSW	16	34027976	missense	probably damaging	1.00
Soulful	UTSW	16	34187484	nonsense	probably null
G1Funyon:Kalrn	UTSW	16	34357100	missense	probably benign	0.05
PIT4498001:Kalrn	UTSW	16	34031582	missense	possibly damaging	0.81
R0019:Kalrn	UTSW	16	34198514	splice site	probably benign
R0043:Kalrn	UTSW	16	34054906	missense	probably damaging	1.00
R0052:Kalrn	UTSW	16	34357171	missense	probably damaging	1.00
R0066:Kalrn	UTSW	16	34203957	missense	probably damaging	1.00
R0098:Kalrn	UTSW	16	33975619	missense	possibly damaging	0.89
R0098:Kalrn	UTSW	16	33975619	missense	possibly damaging	0.89
R0111:Kalrn	UTSW	16	34031590	missense	probably damaging	1.00
R0113:Kalrn	UTSW	16	34049936	intron	probably benign
R0183:Kalrn	UTSW	16	34171379	splice site	probably null
R0422:Kalrn	UTSW	16	34314273	missense	probably damaging	0.99
R0498:Kalrn	UTSW	16	34054891	missense	possibly damaging	0.61
R0614:Kalrn	UTSW	16	33993670	splice site	probably benign
R0656:Kalrn	UTSW	16	34032467	missense	probably damaging	1.00
R0671:Kalrn	UTSW	16	34116408	missense	probably benign	0.04
R0707:Kalrn	UTSW	16	34010581	missense	possibly damaging	0.88
R0709:Kalrn	UTSW	16	34035554	missense	probably damaging	1.00
R0834:Kalrn	UTSW	16	34049919	missense	possibly damaging	0.94
R0976:Kalrn	UTSW	16	34385390	missense	probably damaging	1.00
R1297:Kalrn	UTSW	16	34016498	missense	probably damaging	0.99
R1355:Kalrn	UTSW	16	33975584	missense	possibly damaging	0.74
R1370:Kalrn	UTSW	16	33975584	missense	possibly damaging	0.74
R1389:Kalrn	UTSW	16	33988803	missense	probably benign	0.01
R1398:Kalrn	UTSW	16	34212820	missense	probably damaging	1.00
R1427:Kalrn	UTSW	16	33975754	missense	probably damaging	1.00
R1458:Kalrn	UTSW	16	34174487	missense	probably damaging	1.00
R1470:Kalrn	UTSW	16	34187471	missense	probably damaging	1.00
R1470:Kalrn	UTSW	16	34187471	missense	probably damaging	1.00
R1557:Kalrn	UTSW	16	34314278	missense	possibly damaging	0.92
R1559:Kalrn	UTSW	16	34010548	missense	possibly damaging	0.92
R1654:Kalrn	UTSW	16	33975738	missense	probably damaging	1.00
R1703:Kalrn	UTSW	16	34205326	missense	probably damaging	1.00
R1759:Kalrn	UTSW	16	34360950	missense	probably damaging	0.97
R1764:Kalrn	UTSW	16	34212873	missense	probably damaging	1.00
R1824:Kalrn	UTSW	16	34294215	missense	probably damaging	1.00
R1845:Kalrn	UTSW	16	34356961	missense	probably damaging	0.99
R1850:Kalrn	UTSW	16	33975923	missense	probably damaging	0.98
R1921:Kalrn	UTSW	16	34392093	missense	probably benign	0.02
R1922:Kalrn	UTSW	16	34392093	missense	probably benign	0.02
R1970:Kalrn	UTSW	16	33977524	critical splice donor site	probably null
R1991:Kalrn	UTSW	16	33975738	missense	probably damaging	1.00
R2001:Kalrn	UTSW	16	34028045	missense	probably damaging	1.00
R2025:Kalrn	UTSW	16	34189736	missense	probably damaging	0.96
R2048:Kalrn	UTSW	16	34252310	missense	probably benign	0.18
R2076:Kalrn	UTSW	16	34332143	missense	probably benign	0.15
R2118:Kalrn	UTSW	16	34332230	missense	possibly damaging	0.84
R2136:Kalrn	UTSW	16	34307724	missense	possibly damaging	0.82
R2145:Kalrn	UTSW	16	34009262	unclassified	probably benign
R2193:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2195:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2234:Kalrn	UTSW	16	34176262	splice site	probably null
R2404:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2405:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2408:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2411:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2570:Kalrn	UTSW	16	34310495	missense	probably damaging	1.00
R2903:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2904:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R2924:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R3411:Kalrn	UTSW	16	34212272	missense	probably benign	0.07
R3693:Kalrn	UTSW	16	34357315	missense	probably damaging	1.00
R3709:Kalrn	UTSW	16	34392030	splice site	probably null
R3788:Kalrn	UTSW	16	34220240	missense	probably damaging	1.00
R3833:Kalrn	UTSW	16	34039889	nonsense	probably null
R3871:Kalrn	UTSW	16	34203856	splice site	probably null
R3934:Kalrn	UTSW	16	34310531	missense	probably benign	0.34
R4033:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4056:Kalrn	UTSW	16	34314209	missense	probably damaging	0.99
R4057:Kalrn	UTSW	16	34314209	missense	probably damaging	0.99
R4303:Kalrn	UTSW	16	34235391	missense	probably damaging	1.00
R4402:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4444:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4482:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4487:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4558:Kalrn	UTSW	16	33987208	missense	possibly damaging	0.46
R4572:Kalrn	UTSW	16	34392042	missense	probably damaging	0.98
R4583:Kalrn	UTSW	16	34235267	missense	probably damaging	1.00
R4604:Kalrn	UTSW	16	34513926	missense	possibly damaging	0.46
R4620:Kalrn	UTSW	16	34028705	missense	probably damaging	0.99
R4651:Kalrn	UTSW	16	34176391	missense	probably damaging	1.00
R4703:Kalrn	UTSW	16	34203957	missense	probably damaging	1.00
R4704:Kalrn	UTSW	16	34203957	missense	probably damaging	1.00
R4705:Kalrn	UTSW	16	34203957	missense	probably damaging	1.00
R4760:Kalrn	UTSW	16	34198487	missense	probably damaging	1.00
R4793:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4794:Kalrn	UTSW	16	33989810	missense	possibly damaging	0.72
R4811:Kalrn	UTSW	16	34356969	missense	probably damaging	1.00
R4816:Kalrn	UTSW	16	34514019	unclassified	probably benign
R4888:Kalrn	UTSW	16	34171330	missense	probably damaging	1.00
R4952:Kalrn	UTSW	16	34357415	splice site	probably null
R5030:Kalrn	UTSW	16	33975742	missense	probably benign	0.00
R5045:Kalrn	UTSW	16	34314352	nonsense	probably null
R5117:Kalrn	UTSW	16	34033601	critical splice acceptor site	probably null
R5289:Kalrn	UTSW	16	34252341	missense	possibly damaging	0.95
R5426:Kalrn	UTSW	16	34262653	missense	probably damaging	1.00
R5432:Kalrn	UTSW	16	34053622	missense	probably damaging	1.00
R5611:Kalrn	UTSW	16	34175780	missense	probably damaging	1.00
R5629:Kalrn	UTSW	16	34039934	missense	possibly damaging	0.77
R5635:Kalrn	UTSW	16	34014084	missense	probably damaging	1.00
R5713:Kalrn	UTSW	16	34016579	missense	probably benign
R5716:Kalrn	UTSW	16	33987176	missense	probably benign	0.01
R5772:Kalrn	UTSW	16	33975820	missense	probably damaging	1.00
R5797:Kalrn	UTSW	16	34212249	missense	probably damaging	0.98
R5835:Kalrn	UTSW	16	33987091	missense	probably benign	0.28
R5895:Kalrn	UTSW	16	33975435	utr 3 prime	probably benign
R5924:Kalrn	UTSW	16	34243833	missense	probably damaging	1.00
R5999:Kalrn	UTSW	16	34357343	missense	probably damaging	1.00
R6010:Kalrn	UTSW	16	34010580	missense	probably benign	0.06
R6052:Kalrn	UTSW	16	34360885	missense	probably damaging	1.00
R6122:Kalrn	UTSW	16	33985191	missense	possibly damaging	0.82
R6128:Kalrn	UTSW	16	34212885	missense	probably damaging	0.99
R6136:Kalrn	UTSW	16	34357111	missense	probably damaging	1.00
R6178:Kalrn	UTSW	16	34053639	missense	possibly damaging	0.88
R6229:Kalrn	UTSW	16	34055071	missense	probably damaging	1.00
R6376:Kalrn	UTSW	16	33975991	missense	probably benign
R6397:Kalrn	UTSW	16	33992985	missense	probably damaging	1.00
R6429:Kalrn	UTSW	16	34332164	missense	possibly damaging	0.85
R6473:Kalrn	UTSW	16	34205302	missense	probably damaging	1.00
R6481:Kalrn	UTSW	16	34360984	missense	probably damaging	1.00
R6597:Kalrn	UTSW	16	34182747	missense	probably damaging	1.00
R6736:Kalrn	UTSW	16	34217923	missense	probably damaging	1.00
R6808:Kalrn	UTSW	16	34027976	missense	probably damaging	1.00
R6897:Kalrn	UTSW	16	33975703	missense	probably damaging	0.99
R6955:Kalrn	UTSW	16	34220136	missense	probably damaging	1.00
R7060:Kalrn	UTSW	16	34357048	missense	probably damaging	0.99
R7064:Kalrn	UTSW	16	34217891	missense	probably damaging	1.00
R7132:Kalrn	UTSW	16	34256227	missense	unknown
R7154:Kalrn	UTSW	16	34212157	critical splice donor site	probably null
R7181:Kalrn	UTSW	16	34163077	missense	probably benign	0.00
R7234:Kalrn	UTSW	16	34176422	missense	possibly damaging	0.63
R7235:Kalrn	UTSW	16	34175761	missense	probably benign	0.18
R7504:Kalrn	UTSW	16	34256233	missense	unknown
R7563:Kalrn	UTSW	16	34392094	missense	probably damaging	0.97
R7612:Kalrn	UTSW	16	34314212	missense	possibly damaging	0.68
R7772:Kalrn	UTSW	16	34031582	missense	probably benign	0.04
R7796:Kalrn	UTSW	16	34187484	nonsense	probably null
R7867:Kalrn	UTSW	16	33989791	missense	possibly damaging	0.94
R7869:Kalrn	UTSW	16	33988847	missense	probably damaging	0.98
R7914:Kalrn	UTSW	16	34028752	missense	probably benign
R8080:Kalrn	UTSW	16	33975668	missense	possibly damaging	0.83
R8147:Kalrn	UTSW	16	34055044	missense	probably benign
R8239:Kalrn	UTSW	16	34049783	missense	noncoding transcript
R8281:Kalrn	UTSW	16	34035061	nonsense	probably null
R8294:Kalrn	UTSW	16	34033584	missense	probably benign	0.12
R8301:Kalrn	UTSW	16	34357100	missense	probably benign	0.05
R8686:Kalrn	UTSW	16	34360935	missense	probably damaging	1.00
R8693:Kalrn	UTSW	16	34034514	missense	probably damaging	1.00
R8798:Kalrn	UTSW	16	33982855	missense	possibly damaging	0.65
R8878:Kalrn	UTSW	16	34198460	missense	probably benign	0.05
R8878:Kalrn	UTSW	16	34205326	missense	probably damaging	1.00
R8880:Kalrn	UTSW	16	34217935	missense	probably damaging	1.00
R8883:Kalrn	UTSW	16	33993655	missense	probably damaging	1.00
R8887:Kalrn	UTSW	16	34227126	missense	probably benign	0.22
R9048:Kalrn	UTSW	16	34034484	missense	possibly damaging	0.84
R9111:Kalrn	UTSW	16	34361001	missense	probably damaging	0.96
R9317:Kalrn	UTSW	16	34013675	missense
R9424:Kalrn	UTSW	16	33988818	missense	probably benign	0.06
R9442:Kalrn	UTSW	16	34095879	start codon destroyed	probably null	0.56
R9445:Kalrn	UTSW	16	33985230	missense	probably benign	0.13
R9515:Kalrn	UTSW	16	34034494	missense	probably damaging	1.00
R9516:Kalrn	UTSW	16	34034494	missense	probably damaging	1.00
R9625:Kalrn	UTSW	16	34028827	critical splice acceptor site	probably null
R9645:Kalrn	UTSW	16	34212213	missense	probably benign	0.01
RF014:Kalrn	UTSW	16	34039933	missense	probably benign	0.01
Z1177:Kalrn	UTSW	16	34035506	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTTCACCCTATTGACGATG -3'
(R):5'- AGTGATCCAAGGCATCCCTG -3'

Sequencing Primer
(F):5'- ACGATGTAACTCTTGACATTGGG -3'
(R):5'- GATCCAAGGCATCCCTGTCTCC -3'

Posted On

2014-08-25