Mutagenetix > Incidental Mutation

Incidental Mutation 'R0142:Or2ag15'

22380

Institutional Source

Beutler Lab

Gene Symbol

Or2ag15

Ensembl Gene

ENSMUSG00000051591

Gene Name

olfactory receptor family 2 subfamily AG member 15

Synonyms

GA_x6K02T2PBJ9-9119301-9118348, Olfr697, MOR283-5

MMRRC Submission

038427-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R0142 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

106340112-106341163 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 106340972 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 56 (H56Q)

Ref Sequence

ENSEMBL: ENSMUSP00000152039 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050541] [ENSMUST00000217734]

AlphaFold

Q8VFM4

Predicted Effect

probably benign
Transcript: ENSMUST00000050541
AA Change: H56Q

PolyPhen 2 Score 0.361 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000055207
Gene: ENSMUSG00000051591
AA Change: H56Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.2e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	305	4.7e-7	PFAM
Pfam:7tm_1	41	290	8.7e-26	PFAM
Pfam:7TM_GPCR_Srx	69	306	4.3e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217734
AA Change: H56Q

PolyPhen 2 Score 0.361 (Sensitivity: 0.90; Specificity: 0.89)

Meta Mutation Damage Score

0.3512

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 87.6%

Validation Efficiency

92% (61/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	T	G	11: 110,079,467 (GRCm39)	D1229A	probably damaging	Het
Abhd8	A	C	8: 71,914,506 (GRCm39)	F41V	probably damaging	Het
Ago4	T	G	4: 126,410,725 (GRCm39)	E222A	probably benign	Het
Ap3b2	T	C	7: 81,122,828 (GRCm39)	I470V	probably damaging	Het
Bcl9l	C	T	9: 44,418,409 (GRCm39)	T749M	probably benign	Het
Bicc1	T	C	10: 70,761,200 (GRCm39)	K937E	probably damaging	Het
Bmi1	G	A	2: 18,688,095 (GRCm39)		probably null	Het
Boc	A	C	16: 44,310,604 (GRCm39)	I772S	probably damaging	Het
Brd10	A	G	19: 29,695,654 (GRCm39)	S1347P	possibly damaging	Het
C2	T	A	17: 35,092,504 (GRCm39)	I178F	possibly damaging	Het
Cacna1c	G	T	6: 118,580,843 (GRCm39)	A1416E	probably damaging	Het
Chst10	A	G	1: 38,910,810 (GRCm39)	L118P	probably damaging	Het
Crybg1	G	A	10: 43,875,059 (GRCm39)	T683I	possibly damaging	Het
Cul5	C	T	9: 53,546,350 (GRCm39)	V314I	probably damaging	Het
Dnajc17	C	A	2: 119,010,415 (GRCm39)	R211I	probably benign	Het
Emilin1	A	G	5: 31,071,264 (GRCm39)	T16A	probably benign	Het
Ercc6l2	A	C	13: 64,020,320 (GRCm39)		probably benign	Het
Fsd2	T	A	7: 81,209,683 (GRCm39)	D53V	probably damaging	Het
Galnt13	A	G	2: 54,988,615 (GRCm39)	D479G	probably damaging	Het
Grk3	A	T	5: 113,062,919 (GRCm39)	W643R	probably damaging	Het
Hdgf	G	A	3: 87,820,416 (GRCm39)	A4T	possibly damaging	Het
Hnrnpr	T	A	4: 136,054,593 (GRCm39)	V182E	probably damaging	Het
Ipo13	A	C	4: 117,762,766 (GRCm39)	L279R	probably damaging	Het
Itga9	C	A	9: 118,465,654 (GRCm39)	N169K	probably damaging	Het
Jph3	A	G	8: 122,480,110 (GRCm39)	T263A	possibly damaging	Het
Jph4	G	T	14: 55,345,783 (GRCm39)	Q625K	probably benign	Het
Kctd3	A	C	1: 188,728,595 (GRCm39)		probably null	Het
Kif26b	A	T	1: 178,742,954 (GRCm39)	S570C	probably damaging	Het
Klhl5	G	A	5: 65,300,693 (GRCm39)	W164*	probably null	Het
Lacc1	A	T	14: 77,268,239 (GRCm39)	H357Q	probably benign	Het
Lama2	A	G	10: 27,063,841 (GRCm39)	I1316T	probably benign	Het
Lcp2	C	T	11: 34,032,418 (GRCm39)	P332L	probably damaging	Het
Map3k6	A	T	4: 132,978,257 (GRCm39)	H1033L	probably benign	Het
Mfsd2b	A	G	12: 4,916,234 (GRCm39)	V252A	probably benign	Het
Myo16	T	A	8: 10,619,790 (GRCm39)	I1447N	probably benign	Het
Myo19	G	A	11: 84,785,429 (GRCm39)	R224H	probably damaging	Het
Myo5a	C	T	9: 75,067,856 (GRCm39)	H637Y	probably benign	Het
Nek10	C	T	14: 14,861,560 (GRCm38)	R539C	possibly damaging	Het
Nfix	A	T	8: 85,448,315 (GRCm39)	V404E	probably damaging	Het
Nr1i2	T	C	16: 38,073,368 (GRCm39)	R203G	probably benign	Het
Nup210l	G	A	3: 90,079,420 (GRCm39)	G968D	probably damaging	Het
Or10q1	A	T	19: 13,726,619 (GRCm39)	I50F	probably benign	Het
Or8b37	A	T	9: 37,959,406 (GRCm39)	H296L	probably benign	Het
Phlpp2	C	T	8: 110,634,145 (GRCm39)	R242W	probably damaging	Het
Plcz1	A	G	6: 139,953,423 (GRCm39)	F398S	probably damaging	Het
Ppfibp2	C	A	7: 107,343,384 (GRCm39)	P808T	probably damaging	Het
Srpk2	T	C	5: 23,732,928 (GRCm39)	K239E	probably damaging	Het
Svep1	A	G	4: 58,118,232 (GRCm39)	V830A	probably benign	Het
Tesc	A	T	5: 118,194,635 (GRCm39)	I149F	possibly damaging	Het
Thsd7a	A	G	6: 12,418,334 (GRCm39)	W632R	probably damaging	Het
Tmprss9	A	G	10: 80,730,212 (GRCm39)	D704G	possibly damaging	Het
Tob1	T	C	11: 94,105,423 (GRCm39)	Y320H	probably damaging	Het
Trpm3	G	T	19: 22,965,280 (GRCm39)	D1582Y	probably damaging	Het
Ttc28	A	G	5: 111,425,323 (GRCm39)	K1716R	probably benign	Het
Uqcrfs1	A	G	13: 30,724,925 (GRCm39)	V205A	probably benign	Het
Usp29	G	A	7: 6,965,334 (GRCm39)	M392I	probably benign	Het
Uspl1	A	T	5: 149,125,159 (GRCm39)	Y22F	possibly damaging	Het
Virma	C	A	4: 11,548,783 (GRCm39)	N1780K	probably benign	Het
Vmn1r56	C	T	7: 5,199,372 (GRCm39)	A82T	probably benign	Het
Vmn2r5	A	T	3: 64,400,009 (GRCm39)	C553S	probably damaging	Het
Vwce	A	T	19: 10,641,976 (GRCm39)	R901W	probably damaging	Het
Wdpcp	C	A	11: 21,807,444 (GRCm39)		probably null	Het
Zfp423	A	T	8: 88,506,968 (GRCm39)	C1000*	probably null	Het
Zscan20	A	G	4: 128,479,630 (GRCm39)	F954L	probably benign	Het

Other mutations in Or2ag15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00226:Or2ag15	APN	7	106,340,908 (GRCm39)	missense	probably benign	0.20
IGL00937:Or2ag15	APN	7	106,340,364 (GRCm39)	missense	probably damaging	1.00
IGL01368:Or2ag15	APN	7	106,340,829 (GRCm39)	missense	probably benign	0.19
IGL01410:Or2ag15	APN	7	106,340,706 (GRCm39)	missense	probably benign	0.19
IGL01415:Or2ag15	APN	7	106,340,706 (GRCm39)	missense	probably benign	0.19
IGL01962:Or2ag15	APN	7	106,340,991 (GRCm39)	missense	probably benign	0.12
IGL02654:Or2ag15	APN	7	106,340,555 (GRCm39)	nonsense	probably null
IGL02903:Or2ag15	APN	7	106,340,917 (GRCm39)	missense	probably damaging	1.00
IGL03347:Or2ag15	APN	7	106,340,177 (GRCm39)	utr 3 prime	probably benign
IGL03391:Or2ag15	APN	7	106,340,962 (GRCm39)	missense	probably damaging	1.00
R0139:Or2ag15	UTSW	7	106,340,832 (GRCm39)	missense	probably benign	0.05
R1293:Or2ag15	UTSW	7	106,341,058 (GRCm39)	missense	probably damaging	0.98
R1522:Or2ag15	UTSW	7	106,340,212 (GRCm39)	missense	probably benign	0.03
R1715:Or2ag15	UTSW	7	106,340,755 (GRCm39)	missense	probably damaging	1.00
R1959:Or2ag15	UTSW	7	106,340,601 (GRCm39)	missense	probably damaging	1.00
R1960:Or2ag15	UTSW	7	106,340,601 (GRCm39)	missense	probably damaging	1.00
R2031:Or2ag15	UTSW	7	106,341,105 (GRCm39)	missense	probably damaging	1.00
R4790:Or2ag15	UTSW	7	106,340,998 (GRCm39)	missense	probably benign	0.05
R5550:Or2ag15	UTSW	7	106,340,340 (GRCm39)	missense	probably benign	0.01
R6232:Or2ag15	UTSW	7	106,340,761 (GRCm39)	missense	probably damaging	0.96
R6293:Or2ag15	UTSW	7	106,340,613 (GRCm39)	missense	probably damaging	1.00
R6643:Or2ag15	UTSW	7	106,340,911 (GRCm39)	missense	probably benign	0.06
R7831:Or2ag15	UTSW	7	106,340,620 (GRCm39)	missense	probably damaging	0.99
R8013:Or2ag15	UTSW	7	106,340,824 (GRCm39)	missense	probably benign	0.00
R8014:Or2ag15	UTSW	7	106,340,824 (GRCm39)	missense	probably benign	0.00
R8883:Or2ag15	UTSW	7	106,340,274 (GRCm39)	missense	possibly damaging	0.74
R9072:Or2ag15	UTSW	7	106,340,759 (GRCm39)	nonsense	probably null
RF018:Or2ag15	UTSW	7	106,340,692 (GRCm39)	missense	probably benign	0.02
X0020:Or2ag15	UTSW	7	106,340,343 (GRCm39)	missense	probably damaging	0.97
Z1088:Or2ag15	UTSW	7	106,340,350 (GRCm39)	missense	probably benign	0.21

Predicted Primers

PCR Primer
(F):5'- ACCAATTCAATGAACATCTGAAGGGCAC -3'
(R):5'- AACATGAAGACCTCTAAGCATCCAATGG -3'

Sequencing Primer
(F):5'- ATCTGAAGGGCACAGCCTC -3'
(R):5'- ATAGGGTAGAGCTGAGTATCATTTCC -3'

Posted On

2013-04-16