|Institutional Source||Beutler Lab|
|Gene Name||eukaryotic translation initiation factor 2-alpha kinase 2|
|Synonyms||dsRNA-activated kinase, eIF-2 alpha, eIF-2 alpha, IFN- type I-induced and dsRNA-activated kinase, IFN-induced and double-stranded RNA-activated kinase, Pkr, Prkr, Tik|
|Is this an essential gene?||Possibly essential (E-score: 0.607)|
|Stock #||R2020 (G1)|
|Chromosomal Location||78852564-78882573 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 78863963 bp (GRCm38)|
|Amino Acid Change||Glutamic Acid to Glycine at position 337 (E337G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000024884 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000024884]|
Solution structure of the second DSRM domain in Interferon-induced, double-stranded RNA-activated protein kinase [SOLUTION NMR]
Solution structure of the first DSRM domain in Interferon-induced, double-stranded RNA-activated protein kinase [SOLUTION NMR]
AA Change: E337G
PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)
AA Change: E337G
|Meta Mutation Damage Score||0.2286|
|Coding Region Coverage||
|Validation Efficiency||99% (87/88)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for disruptions in this gene display altered susceptibility to viral infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Eif2ak2||
(F):5'- ATCGTGACTAACTGCCAATCAC -3'
(R):5'- CAATCGAAGCTCTGGATGCTTG -3'
(F):5'- TCGTGACTAACTGCCAATCACAAATC -3'
(R):5'- CTCTGGATGCTTGGGAAATTATGAC -3'