Mutagenetix > Incidental Mutation

Incidental Mutation 'R2042:Fbxw26'

225098

Institutional Source

Beutler Lab

Gene Symbol

Fbxw26

Ensembl Gene

ENSMUSG00000059547

Gene Name

F-box and WD-40 domain protein 26

Synonyms

Gm5163

MMRRC Submission

040049-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2042 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109717566-109746089 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 109732704 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 141 (T141P)

Ref Sequence

ENSEMBL: ENSMUSP00000071811 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071917]

AlphaFold

Q8BI58

Predicted Effect

probably damaging
Transcript: ENSMUST00000071917
AA Change: T141P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000071811
Gene: ENSMUSG00000059547
AA Change: T141P

Domain	Start	End	E-Value	Type
FBOX	5	45	2.54e-6	SMART
SCOP:d1tbga_	128	249	1e-5	SMART
Blast:WD40	137	176	4e-7	BLAST

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

100% (67/67)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	A	3: 124,416,728 (GRCm38)		probably benign	Het
9130019O22Rik	A	T	7: 127,385,469 (GRCm38)	C154S	possibly damaging	Het
Abca16	G	A	7: 120,544,718 (GRCm38)	R1653Q	probably benign	Het
Ahnak2	T	A	12: 112,785,819 (GRCm38)	Y176F	probably damaging	Het
Ano6	T	C	15: 95,956,023 (GRCm38)		probably null	Het
Atr	T	C	9: 95,870,022 (GRCm38)	L564S	probably benign	Het
Birc6	C	G	17: 74,609,659 (GRCm38)	A1774G	probably damaging	Het
Cacng1	C	T	11: 107,704,308 (GRCm38)	A148T	probably damaging	Het
Cd53	T	A	3: 106,767,424 (GRCm38)		probably null	Het
Celsr2	A	G	3: 108,402,495 (GRCm38)	F1596S	probably damaging	Het
Cep120	A	T	18: 53,735,742 (GRCm38)	F122I	possibly damaging	Het
Ckm	A	T	7: 19,414,157 (GRCm38)	H7L	possibly damaging	Het
Crybg2	T	C	4: 134,087,533 (GRCm38)	V1575A	possibly damaging	Het
Cspp1	A	G	1: 10,112,538 (GRCm38)	E712G	probably damaging	Het
Cyp2b23	C	A	7: 26,666,108 (GRCm38)	R434L	probably damaging	Het
D630003M21Rik	A	T	2: 158,215,849 (GRCm38)	S570T	probably damaging	Het
Dmbt1	A	G	7: 131,106,359 (GRCm38)	I1444V	probably damaging	Het
Dnah8	T	C	17: 30,635,658 (GRCm38)	V98A	probably benign	Het
Dtx1	T	G	5: 120,694,476 (GRCm38)	N299T	probably benign	Het
Efr3b	T	A	12: 3,984,627 (GRCm38)	D65V	probably damaging	Het
Eml4	T	C	17: 83,448,178 (GRCm38)	C323R	probably damaging	Het
Eps15	C	T	4: 109,304,767 (GRCm38)	T31I	probably damaging	Het
Fam160a2	G	T	7: 105,384,121 (GRCm38)	Y629*	probably null	Het
Fam205c	C	T	4: 42,874,030 (GRCm38)	C46Y	possibly damaging	Het
Fam46b	T	C	4: 133,486,613 (GRCm38)	V265A	possibly damaging	Het
Fam91a1	A	G	15: 58,426,594 (GRCm38)	I184V	probably benign	Het
Fbxl8	A	T	8: 105,268,224 (GRCm38)	I123F	probably damaging	Het
Glra3	G	A	8: 56,062,459 (GRCm38)	D190N	probably benign	Het
Hspg2	T	C	4: 137,568,366 (GRCm38)	L4229P	probably damaging	Het
Ipmk	C	T	10: 71,363,503 (GRCm38)	R65W	probably damaging	Het
Irs2	A	G	8: 11,007,580 (GRCm38)	I284T	probably damaging	Het
Klhl22	T	C	16: 17,792,420 (GRCm38)		probably benign	Het
Lmcd1	T	A	6: 112,315,890 (GRCm38)	D234E	probably benign	Het
Lrrc14b	T	C	13: 74,363,442 (GRCm38)	K173R	probably benign	Het
Magi1	A	T	6: 93,755,045 (GRCm38)	N209K	probably benign	Het
Mak	A	C	13: 41,049,436 (GRCm38)	S179A	possibly damaging	Het
Map3k4	C	A	17: 12,277,983 (GRCm38)	R87L	probably damaging	Het
Map4k1	T	C	7: 28,984,130 (GRCm38)	L53P	probably damaging	Het
Melk	T	C	4: 44,309,051 (GRCm38)		probably null	Het
Mks1	C	T	11: 87,856,668 (GRCm38)		probably benign	Het
Mrgpra2b	C	T	7: 47,464,160 (GRCm38)	V249I	probably benign	Het
N4bp2	C	T	5: 65,826,621 (GRCm38)	P1670S	probably damaging	Het
Ncf1	C	G	5: 134,226,640 (GRCm38)	Q132H	probably benign	Het
Nemp1	T	C	10: 127,696,334 (GRCm38)	S370P	possibly damaging	Het
Nt5c3b	T	C	11: 100,436,194 (GRCm38)	H92R	probably benign	Het
Olfr1180	G	A	2: 88,412,202 (GRCm38)	A152V	possibly damaging	Het
Olfr527	T	C	7: 140,335,937 (GRCm38)	L25P	probably damaging	Het
P4ha3	T	C	7: 100,300,690 (GRCm38)		probably null	Het
Pcnx	C	A	12: 81,918,293 (GRCm38)	H411Q	probably damaging	Het
Podxl	A	G	6: 31,523,116 (GRCm38)	V473A	possibly damaging	Het
Prkd2	T	C	7: 16,856,268 (GRCm38)	S530P	possibly damaging	Het
Scin	A	G	12: 40,077,510 (GRCm38)	I427T	possibly damaging	Het
Sgo2b	T	C	8: 63,928,527 (GRCm38)	T424A	probably benign	Het
Slc22a2	T	C	17: 12,599,125 (GRCm38)	I196T	probably benign	Het
Slc47a2	C	A	11: 61,338,082 (GRCm38)	V90L	probably benign	Het
Slc4a7	G	A	14: 14,737,386 (GRCm38)	V99M	probably damaging	Het
Sprr2k	T	C	3: 92,433,456 (GRCm38)		probably benign	Het
Spta1	G	A	1: 174,211,647 (GRCm38)	M1185I	probably benign	Het
Uaca	T	C	9: 60,869,891 (GRCm38)	V518A	probably damaging	Het
Ubr3	C	T	2: 69,977,774 (GRCm38)	Q1200*	probably null	Het
Ufm1	A	G	3: 53,859,281 (GRCm38)		probably benign	Het
Zer1	G	A	2: 30,108,274 (GRCm38)	L342F	probably damaging	Het
Zfp142	G	A	1: 74,570,619 (GRCm38)	T1236I	probably benign	Het
Zfp236	A	G	18: 82,633,109 (GRCm38)	Y845H	probably damaging	Het
Zfp787	T	C	7: 6,132,764 (GRCm38)	K163E	possibly damaging	Het

Other mutations in Fbxw26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00589:Fbxw26	APN	9	109,717,948 (GRCm38)	utr 3 prime	probably benign
IGL01072:Fbxw26	APN	9	109,723,837 (GRCm38)	missense	probably damaging	1.00
IGL01151:Fbxw26	APN	9	109,721,780 (GRCm38)	missense	possibly damaging	0.50
IGL01394:Fbxw26	APN	9	109,717,989 (GRCm38)	missense	probably benign	0.00
IGL01432:Fbxw26	APN	9	109,717,975 (GRCm38)	missense	probably benign	0.32
IGL02559:Fbxw26	APN	9	109,722,164 (GRCm38)	missense	probably benign	0.34
IGL02981:Fbxw26	APN	9	109,744,794 (GRCm38)	missense	probably benign	0.09
IGL03370:Fbxw26	APN	9	109,746,019 (GRCm38)	missense	probably damaging	1.00
R0023:Fbxw26	UTSW	9	109,718,011 (GRCm38)	missense	probably benign	0.01
R0087:Fbxw26	UTSW	9	109,724,938 (GRCm38)	missense	probably benign
R0369:Fbxw26	UTSW	9	109,723,712 (GRCm38)	critical splice donor site	probably null
R0446:Fbxw26	UTSW	9	109,743,720 (GRCm38)	missense	probably benign	0.03
R1844:Fbxw26	UTSW	9	109,724,878 (GRCm38)	missense	probably benign	0.42
R1891:Fbxw26	UTSW	9	109,722,164 (GRCm38)	missense	probably benign	0.34
R3615:Fbxw26	UTSW	9	109,743,760 (GRCm38)	nonsense	probably null
R3616:Fbxw26	UTSW	9	109,743,760 (GRCm38)	nonsense	probably null
R4659:Fbxw26	UTSW	9	109,744,871 (GRCm38)	missense	probably damaging	0.97
R4785:Fbxw26	UTSW	9	109,724,800 (GRCm38)	missense	possibly damaging	0.50
R4898:Fbxw26	UTSW	9	109,717,969 (GRCm38)	missense	possibly damaging	0.95
R5791:Fbxw26	UTSW	9	109,745,153 (GRCm38)	missense	probably damaging	1.00
R5818:Fbxw26	UTSW	9	109,732,566 (GRCm38)	missense	probably benign
R5921:Fbxw26	UTSW	9	109,746,018 (GRCm38)	missense	probably damaging	1.00
R5983:Fbxw26	UTSW	9	109,717,965 (GRCm38)	missense	possibly damaging	0.49
R6145:Fbxw26	UTSW	9	109,732,623 (GRCm38)	missense	probably benign	0.09
R6209:Fbxw26	UTSW	9	109,717,965 (GRCm38)	missense	possibly damaging	0.49
R6412:Fbxw26	UTSW	9	109,732,647 (GRCm38)	missense	probably damaging	0.97
R6842:Fbxw26	UTSW	9	109,724,920 (GRCm38)	missense	probably damaging	1.00
R7228:Fbxw26	UTSW	9	109,724,944 (GRCm38)	missense	possibly damaging	0.93
R7451:Fbxw26	UTSW	9	109,732,623 (GRCm38)	missense	probably benign	0.03
R7467:Fbxw26	UTSW	9	109,732,697 (GRCm38)	missense	probably benign	0.00
R8397:Fbxw26	UTSW	9	109,732,647 (GRCm38)	missense	probably damaging	0.99
R8912:Fbxw26	UTSW	9	109,732,649 (GRCm38)	missense	probably damaging	1.00
R9284:Fbxw26	UTSW	9	109,721,894 (GRCm38)	intron	probably benign
R9479:Fbxw26	UTSW	9	109,732,557 (GRCm38)	missense	probably damaging	0.99
R9694:Fbxw26	UTSW	9	109,746,067 (GRCm38)	start gained	probably benign
X0020:Fbxw26	UTSW	9	109,732,632 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CGGGCCATCCTTTGTAATGAC -3'
(R):5'- TGTCCATTCCTGCCACAAATAATC -3'

Sequencing Primer
(F):5'- GACAGCTTTCAGTAATTGACAGG -3'
(R):5'- AACAGCTGGTGGTCTTAACC -3'

Posted On

2014-08-25