Mutagenetix > Incidental Mutation

Incidental Mutation 'R2005:Helz2'

225337

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

040014-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2005 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 181231329 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 2396 (R2396H)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094203
AA Change: R2396H

PolyPhen 2 Score 0.392 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: R2396H

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108831
AA Change: R2396H

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: R2396H

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121484
AA Change: R2396H

PolyPhen 2 Score 0.447 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: R2396H

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149417

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	G	A	12: 118,877,827 (GRCm38)	A961V	probably benign	Het
Adcy10	A	G	1: 165,525,022 (GRCm38)	Y475C	probably benign	Het
Adgrb3	T	C	1: 25,111,718 (GRCm38)	M1145V	probably benign	Het
Akr1c21	A	G	13: 4,574,215 (GRCm38)	T23A	probably damaging	Het
Ankrd33b	A	T	15: 31,297,668 (GRCm38)	S314R	probably damaging	Het
Apc	T	C	18: 34,310,909 (GRCm38)		probably null	Het
Atg9a	T	A	1: 75,185,991 (GRCm38)	Q460L	probably benign	Het
Birc3	C	T	9: 7,860,341 (GRCm38)	C292Y	probably damaging	Het
Capn5	T	C	7: 98,129,363 (GRCm38)	I350V	probably benign	Het
Cc2d2a	G	A	5: 43,726,373 (GRCm38)		probably null	Het
Cdh22	T	A	2: 165,180,923 (GRCm38)	E76V	probably damaging	Het
Cdh8	T	C	8: 99,033,471 (GRCm38)		probably null	Het
Cdkn1b	ATTCTTCTTC	ATTCTTCTTCTTC	6: 134,921,956 (GRCm38)		probably benign	Het
Cfap54	T	A	10: 92,884,768 (GRCm38)	D2608V	unknown	Het
Crebrf	T	C	17: 26,742,883 (GRCm38)	V318A	possibly damaging	Het
Cwf19l2	A	T	9: 3,430,720 (GRCm38)	N351Y	possibly damaging	Het
Cyp2c67	A	T	19: 39,643,345 (GRCm38)	M136K	probably damaging	Het
Ddx10	A	G	9: 53,240,475 (GRCm38)		probably null	Het
Ddx47	A	G	6: 135,018,121 (GRCm38)	N92S	probably benign	Het
Ddx50	T	A	10: 62,640,464 (GRCm38)	H306L	probably benign	Het
Dnajc27	A	G	12: 4,097,317 (GRCm38)	T167A	possibly damaging	Het
Dscam	T	G	16: 97,038,920 (GRCm38)	K162N	probably benign	Het
Eif4h	G	A	5: 134,627,677 (GRCm38)	T53I	probably benign	Het
Elmo2	T	C	2: 165,298,279 (GRCm38)	N332S	probably benign	Het
Ergic3	A	G	2: 156,011,108 (GRCm38)	N157S	possibly damaging	Het
F2rl1	G	T	13: 95,513,274 (GRCm38)	R367S	probably damaging	Het
Fbxo36	G	A	1: 84,900,069 (GRCm38)	A151T	probably benign	Het
Foxf2	G	T	13: 31,626,141 (GRCm38)	R21L	unknown	Het
Gmip	T	A	8: 69,814,043 (GRCm38)	H269Q	probably benign	Het
Gng4	T	A	13: 13,806,166 (GRCm38)	V32E	probably benign	Het
Grm3	T	C	5: 9,589,793 (GRCm38)	Y84C	possibly damaging	Het
Icosl	A	T	10: 78,071,953 (GRCm38)	M116L	possibly damaging	Het
Itgb3bp	A	C	4: 99,814,058 (GRCm38)	N16K	probably benign	Het
Kcnt2	A	G	1: 140,553,018 (GRCm38)	M824V	probably damaging	Het
Mki67	T	A	7: 135,698,509 (GRCm38)	K1599*	probably null	Het
Mmp25	G	A	17: 23,640,242 (GRCm38)	R144W	probably damaging	Het
Mmrn1	A	T	6: 60,976,084 (GRCm38)	I450F	possibly damaging	Het
Mroh2b	A	T	15: 4,917,158 (GRCm38)	K480M	probably damaging	Het
Nbn	T	A	4: 15,979,351 (GRCm38)	D445E	probably benign	Het
Npbwr1	T	A	1: 5,916,351 (GRCm38)	S315C	probably damaging	Het
Or10ag56	T	A	2: 87,309,448 (GRCm38)	S240T	probably benign	Het
Or1e19	A	T	11: 73,425,239 (GRCm38)	V248E	probably damaging	Het
Or5d38	A	G	2: 88,124,547 (GRCm38)	L146P	probably damaging	Het
Or7g16	G	A	9: 18,815,505 (GRCm38)	S263L	probably benign	Het
Pard3b	A	T	1: 62,144,891 (GRCm38)	E183D	probably benign	Het
Pde10a	G	A	17: 8,929,091 (GRCm38)		probably null	Het
Pdlim2	C	T	14: 70,164,779 (GRCm38)	R296H	probably damaging	Het
Prdm13	T	C	4: 21,685,695 (GRCm38)		probably benign	Het
Rc3h2	T	A	2: 37,389,753 (GRCm38)	K461*	probably null	Het
Reep2	C	T	18: 34,845,621 (GRCm38)	T117M	probably damaging	Het
Rhbdd1	A	G	1: 82,340,810 (GRCm38)	N138S	probably benign	Het
Rnf19a	A	T	15: 36,241,770 (GRCm38)	F708I	possibly damaging	Het
Robo2	G	A	16: 73,933,115 (GRCm38)	H1069Y	possibly damaging	Het
Rpl9	C	T	5: 65,389,535 (GRCm38)	V112I	probably benign	Het
Sec16a	A	C	2: 26,439,080 (GRCm38)	S974R	probably benign	Het
Sfrp5	A	G	19: 42,198,836 (GRCm38)	M265T	probably benign	Het
Skint7	T	C	4: 111,984,850 (GRCm38)	I305T	probably benign	Het
Slc6a7	T	C	18: 61,001,641 (GRCm38)	D454G	possibly damaging	Het
Slco6c1	A	G	1: 97,081,489 (GRCm38)	I407T	probably damaging	Het
Slfn8	A	T	11: 83,004,150 (GRCm38)	M610K	probably damaging	Het
Spidr	A	G	16: 16,048,049 (GRCm38)	S299P	probably damaging	Het
Sptbn4	C	T	7: 27,366,419 (GRCm38)	W2034*	probably null	Het
Stard9	C	A	2: 120,673,636 (GRCm38)	S221R	probably damaging	Het
Stard9	T	A	2: 120,664,945 (GRCm38)	F100L	possibly damaging	Het
Stc1	T	C	14: 69,031,647 (GRCm38)		probably null	Het
Sun2	T	C	15: 79,726,624 (GRCm38)	E667G	possibly damaging	Het
Tacc2	T	C	7: 130,731,550 (GRCm38)	S511P	probably damaging	Het
Tasp1	T	G	2: 139,977,678 (GRCm38)	K202Q	probably damaging	Het
Tdrd6	A	G	17: 43,628,655 (GRCm38)	F501L	probably damaging	Het
Tgds	A	G	14: 118,130,656 (GRCm38)	V35A	possibly damaging	Het
Tubgcp6	G	A	15: 89,104,166 (GRCm38)	T860I	probably benign	Het
Twf1	C	T	15: 94,585,447 (GRCm38)		probably null	Het
Vangl1	A	G	3: 102,163,466 (GRCm38)	S385P	probably benign	Het
Vmn2r117	A	G	17: 23,477,644 (GRCm38)	I263T	probably damaging	Het
Washc5	A	G	15: 59,341,155 (GRCm38)	L892P	possibly damaging	Het
Wnk4	T	C	11: 101,263,890 (GRCm38)	C302R	probably damaging	Het
Zfp395	A	G	14: 65,388,885 (GRCm38)	E191G	possibly damaging	Het
Zkscan17	A	G	11: 59,492,216 (GRCm38)	L302P	probably damaging	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181,229,702 (GRCm38)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181,233,006 (GRCm38)	nonsense	probably null
IGL00704:Helz2	APN	2	181,234,385 (GRCm38)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181,232,245 (GRCm38)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181,233,977 (GRCm38)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181,232,881 (GRCm38)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181,238,481 (GRCm38)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181,232,185 (GRCm38)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181,231,690 (GRCm38)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181,230,911 (GRCm38)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181,231,022 (GRCm38)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181,235,026 (GRCm38)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181,231,146 (GRCm38)	intron	probably benign
IGL03003:Helz2	APN	2	181,240,253 (GRCm38)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181,229,222 (GRCm38)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181,231,804 (GRCm38)	missense	probably benign	0.00
Colby	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181,234,834 (GRCm38)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181,240,959 (GRCm38)	missense	probably benign
R0013:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181,237,802 (GRCm38)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181,232,269 (GRCm38)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181,230,593 (GRCm38)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181,232,209 (GRCm38)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181,229,656 (GRCm38)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181,227,770 (GRCm38)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181,234,825 (GRCm38)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181,232,089 (GRCm38)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181,232,116 (GRCm38)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181,240,881 (GRCm38)	missense	probably benign
R0826:Helz2	UTSW	2	181,240,853 (GRCm38)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181,230,777 (GRCm38)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181,236,135 (GRCm38)	missense	probably benign
R1170:Helz2	UTSW	2	181,229,815 (GRCm38)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181,231,128 (GRCm38)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181,237,596 (GRCm38)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181,232,981 (GRCm38)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181,235,524 (GRCm38)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181,232,804 (GRCm38)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181,233,228 (GRCm38)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181,234,147 (GRCm38)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181,236,263 (GRCm38)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181,238,459 (GRCm38)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	181,234,987 (GRCm38)	missense	probably benign
R1837:Helz2	UTSW	2	181,229,289 (GRCm38)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181,232,085 (GRCm38)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181,234,289 (GRCm38)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181,233,750 (GRCm38)	missense	probably damaging	1.00
R2034:Helz2	UTSW	2	181,232,578 (GRCm38)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181,237,479 (GRCm38)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181,240,544 (GRCm38)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181,235,102 (GRCm38)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181,231,380 (GRCm38)	missense	probably benign
R2180:Helz2	UTSW	2	181,233,732 (GRCm38)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181,229,048 (GRCm38)	nonsense	probably null
R2248:Helz2	UTSW	2	181,233,433 (GRCm38)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181,232,912 (GRCm38)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181,240,742 (GRCm38)	missense	probably benign
R3617:Helz2	UTSW	2	181,233,061 (GRCm38)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181,240,389 (GRCm38)	nonsense	probably null
R3803:Helz2	UTSW	2	181,239,996 (GRCm38)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181,229,710 (GRCm38)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181,240,475 (GRCm38)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181,229,902 (GRCm38)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181,228,833 (GRCm38)	missense	probably benign
R4624:Helz2	UTSW	2	181,239,308 (GRCm38)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181,238,417 (GRCm38)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181,237,417 (GRCm38)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181,230,120 (GRCm38)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181,236,147 (GRCm38)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181,232,438 (GRCm38)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181,240,916 (GRCm38)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181,240,569 (GRCm38)	missense	probably benign
R5089:Helz2	UTSW	2	181,235,149 (GRCm38)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181,230,757 (GRCm38)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181,234,846 (GRCm38)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181,235,528 (GRCm38)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R5559:Helz2	UTSW	2	181,230,126 (GRCm38)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181,240,258 (GRCm38)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181,237,289 (GRCm38)	intron	probably benign
R5805:Helz2	UTSW	2	181,240,508 (GRCm38)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181,236,396 (GRCm38)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181,232,656 (GRCm38)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181,234,028 (GRCm38)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181,230,384 (GRCm38)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181,230,767 (GRCm38)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181,231,050 (GRCm38)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181,240,313 (GRCm38)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181,233,038 (GRCm38)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181,232,294 (GRCm38)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181,235,945 (GRCm38)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R6581:Helz2	UTSW	2	181,229,379 (GRCm38)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181,239,557 (GRCm38)	nonsense	probably null
R6964:Helz2	UTSW	2	181,230,428 (GRCm38)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181,240,514 (GRCm38)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181,231,285 (GRCm38)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181,238,423 (GRCm38)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181,235,600 (GRCm38)	splice site	probably null
R7512:Helz2	UTSW	2	181,230,854 (GRCm38)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	181,237,572 (GRCm38)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	181,231,996 (GRCm38)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181,230,355 (GRCm38)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181,234,531 (GRCm38)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181,233,991 (GRCm38)	missense	probably benign
R7799:Helz2	UTSW	2	181,237,989 (GRCm38)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181,232,902 (GRCm38)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181,237,750 (GRCm38)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181,240,205 (GRCm38)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	181,237,896 (GRCm38)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	181,238,262 (GRCm38)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181,238,102 (GRCm38)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181,230,157 (GRCm38)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181,229,557 (GRCm38)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181,232,767 (GRCm38)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181,233,127 (GRCm38)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181,238,380 (GRCm38)	missense
R8953:Helz2	UTSW	2	181,233,091 (GRCm38)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181,229,614 (GRCm38)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181,237,788 (GRCm38)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181,234,693 (GRCm38)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181,228,999 (GRCm38)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181,232,468 (GRCm38)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181,240,175 (GRCm38)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181,239,640 (GRCm38)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181,240,055 (GRCm38)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181,230,090 (GRCm38)	missense	probably benign
R9186:Helz2	UTSW	2	181,234,664 (GRCm38)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181,240,948 (GRCm38)	missense	probably benign
R9407:Helz2	UTSW	2	181,240,182 (GRCm38)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181,232,917 (GRCm38)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181,236,452 (GRCm38)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181,240,221 (GRCm38)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181,240,677 (GRCm38)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181,240,232 (GRCm38)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181,240,823 (GRCm38)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181,231,741 (GRCm38)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181,237,564 (GRCm38)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181,235,961 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCAGAAGCCAAGGATCATC -3'
(R):5'- AAGACATAGCTCCCTCCCTAGG -3'

Sequencing Primer
(F):5'- CAAGGATCATCTGAGCACCGTG -3'
(R):5'- GGAAAACCCTATTCACTTTCTGG -3'

Posted On

2014-08-25