Mutagenetix > Incidental Mutation

Incidental Mutation 'R2051:Fbxo43'

226466

Institutional Source

Beutler Lab

Gene Symbol

Fbxo43

Ensembl Gene

ENSMUSG00000048230

Gene Name

F-box protein 43

Synonyms

Emi2, early mitotic inhibitor 2, endogenous meiotic inhibitor 2, XErp1 homolog, 4930533G20Rik

MMRRC Submission

040058-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.216) question?

Stock #

R2051 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36150206-36165030 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 36162278 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Tryptophan at position 310 (G310W)

Ref Sequence

ENSEMBL: ENSMUSP00000054125 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058643] [ENSMUST00000227793]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000058643
AA Change: G310W

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000054125
Gene: ENSMUSG00000048230
AA Change: G310W

Domain	Start	End	E-Value	Type
low complexity region	88	100	N/A	INTRINSIC
low complexity region	393	406	N/A	INTRINSIC
Blast:FBOX	439	479	2e-14	BLAST
low complexity region	502	515	N/A	INTRINSIC
IBR	555	614	1.46e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227781

Predicted Effect

probably benign
Transcript: ENSMUST00000227793

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	G	A	5: 138,562,447 (GRCm39)	T98M	probably damaging	Het
4932414N04Rik	G	T	2: 68,541,392 (GRCm39)	K10N	possibly damaging	Het
Aadat	T	A	8: 60,960,173 (GRCm39)	S40T	probably benign	Het
Abca13	A	G	11: 9,278,098 (GRCm39)	I3093V	probably benign	Het
Acacb	A	T	5: 114,383,951 (GRCm39)	Q2160L	probably damaging	Het
Acp6	G	T	3: 97,075,333 (GRCm39)	S189I	probably benign	Het
Actr5	A	T	2: 158,474,213 (GRCm39)	M339L	probably benign	Het
Adcy1	A	T	11: 7,111,885 (GRCm39)	K917*	probably null	Het
Adgrg5	C	T	8: 95,668,695 (GRCm39)	R504C	probably benign	Het
Ago1	T	C	4: 126,354,246 (GRCm39)	H188R	probably benign	Het
Akap9	T	A	5: 4,025,685 (GRCm39)	C23*	probably null	Het
Ank3	T	C	10: 69,733,920 (GRCm39)	I728T	probably damaging	Het
Ankrd50	A	G	3: 38,508,642 (GRCm39)	S1242P	probably benign	Het
Arhgap29	G	A	3: 121,775,509 (GRCm39)	R84H	probably benign	Het
Arhgef10	A	G	8: 14,995,320 (GRCm39)	D7G	probably null	Het
Arid4b	A	G	13: 14,362,230 (GRCm39)	E898G	probably damaging	Het
Atrnl1	A	G	19: 57,680,281 (GRCm39)	N727S	probably benign	Het
Baalc	G	T	15: 38,796,629 (GRCm39)		probably benign	Het
Cdc25c	G	C	18: 34,871,292 (GRCm39)	L275V	probably damaging	Het
Chpf2	G	T	5: 24,796,274 (GRCm39)	V407L	probably benign	Het
Chrnb3	C	A	8: 27,876,839 (GRCm39)	N84K	probably damaging	Het
Cnot1	A	G	8: 96,451,221 (GRCm39)	F2171L	possibly damaging	Het
Csmd3	C	T	15: 48,485,389 (GRCm39)		probably null	Het
Cux1	T	A	5: 136,361,512 (GRCm39)	Q138L	probably damaging	Het
Cyp2c65	A	G	19: 39,070,675 (GRCm39)	N286S	probably benign	Het
Dclre1b	T	C	3: 103,716,356 (GRCm39)	S17G	possibly damaging	Het
Dlgap5	A	G	14: 47,648,941 (GRCm39)	S221P	probably benign	Het
Dnah1	T	G	14: 31,001,080 (GRCm39)	T2422P	probably damaging	Het
Enpp1	A	G	10: 24,587,702 (GRCm39)		probably null	Het
Erbb2	T	C	11: 98,310,998 (GRCm39)	C53R	probably damaging	Het
Exoc8	A	G	8: 125,622,219 (GRCm39)	V716A	probably benign	Het
Fam193a	T	A	5: 34,619,494 (GRCm39)	D766E	probably benign	Het
Fcgbp	C	T	7: 27,819,785 (GRCm39)	T2504I	probably damaging	Het
Fnbp4	C	T	2: 90,587,876 (GRCm39)	P418L	probably benign	Het
Gjd2	T	C	2: 113,841,539 (GRCm39)	T313A	probably damaging	Het
Gm12695	T	A	4: 96,658,008 (GRCm39)	R54W	probably damaging	Het
Gm128	T	C	3: 95,148,051 (GRCm39)	D81G	possibly damaging	Het
Gm21834	A	G	17: 58,048,763 (GRCm39)	V151A	possibly damaging	Het
Grhl1	T	A	12: 24,636,151 (GRCm39)		probably null	Het
Hcn1	C	T	13: 118,112,619 (GRCm39)	T861I	probably damaging	Het
Herc6	C	A	6: 57,602,961 (GRCm39)	Q547K	probably benign	Het
Iqgap3	T	C	3: 88,027,474 (GRCm39)	L699P	probably damaging	Het
Kank4	T	C	4: 98,668,339 (GRCm39)	D36G	probably damaging	Het
Kcnk5	A	T	14: 20,192,277 (GRCm39)	S295T	probably damaging	Het
Krt18	T	C	15: 101,937,935 (GRCm39)	V144A	probably benign	Het
Krtap9-5	A	G	11: 99,840,030 (GRCm39)	I244V	unknown	Het
Leng1	T	G	7: 3,668,400 (GRCm39)	N16T	probably damaging	Het
Lss	A	G	10: 76,367,712 (GRCm39)	K15E	possibly damaging	Het
Mastl	G	T	2: 23,022,836 (GRCm39)	A629E	possibly damaging	Het
Mavs	G	C	2: 131,082,370 (GRCm39)	A85P	possibly damaging	Het
Nav3	T	C	10: 109,660,536 (GRCm39)	D678G	probably damaging	Het
Nsd3	T	G	8: 26,181,116 (GRCm39)	S906A	probably damaging	Het
Nsfl1c	A	G	2: 151,345,002 (GRCm39)	N118S	probably damaging	Het
Nup205	T	A	6: 35,207,451 (GRCm39)	M1501K	probably benign	Het
Or4p7	C	T	2: 88,221,882 (GRCm39)	T97M	possibly damaging	Het
Or6n1	T	A	1: 173,916,785 (GRCm39)	Y60N	possibly damaging	Het
Pax8	A	G	2: 24,326,520 (GRCm39)	S281P	probably benign	Het
Pds5b	T	G	5: 150,671,655 (GRCm39)	I433R	probably damaging	Het
Peg3	T	C	7: 6,715,720 (GRCm39)	N117D	probably damaging	Het
Pfkm	A	T	15: 98,029,573 (GRCm39)	D728V	probably benign	Het
Phkb	T	C	8: 86,776,450 (GRCm39)		probably null	Het
Pkp4	T	C	2: 59,165,248 (GRCm39)	V704A	probably benign	Het
Ppfia2	T	A	10: 106,673,160 (GRCm39)	S501T	probably damaging	Het
Ptpru	T	C	4: 131,546,398 (GRCm39)	E284G	possibly damaging	Het
Ror1	C	T	4: 100,265,065 (GRCm39)	R180*	probably null	Het
Ryr3	T	A	2: 112,586,986 (GRCm39)	Y2666F	probably damaging	Het
Sec23a	C	A	12: 59,037,754 (GRCm39)		probably null	Het
Sertad3	C	T	7: 27,175,694 (GRCm39)	Q43*	probably null	Het
Setd2	C	T	9: 110,379,958 (GRCm39)	H1258Y	probably benign	Het
Sharpin	A	G	15: 76,232,407 (GRCm39)	S177P	probably benign	Het
Skap1	T	C	11: 96,432,289 (GRCm39)	F86S	possibly damaging	Het
Slc8a2	T	C	7: 15,874,940 (GRCm39)	I396T	probably damaging	Het
Slc9a2	T	C	1: 40,765,597 (GRCm39)	F329S	probably damaging	Het
Slx4ip	C	T	2: 136,908,125 (GRCm39)	L161F	possibly damaging	Het
Sox4	A	G	13: 29,136,764 (GRCm39)	S81P	probably damaging	Het
Ssc4d	G	T	5: 135,999,118 (GRCm39)	S28R	probably benign	Het
St8sia2	C	T	7: 73,592,950 (GRCm39)	G369S	possibly damaging	Het
Swt1	T	C	1: 151,248,081 (GRCm39)	Y836C	probably damaging	Het
Taar7d	A	G	10: 23,903,904 (GRCm39)	D262G	probably benign	Het
Taar8b	T	A	10: 23,967,212 (GRCm39)	L327F	probably benign	Het
Tars1	A	T	15: 11,393,280 (GRCm39)	L138*	probably null	Het
Tbcd	A	T	11: 121,344,496 (GRCm39)	D75V	probably damaging	Het
Tesc	A	G	5: 118,184,394 (GRCm39)	I25V	probably damaging	Het
Tmem132e	G	T	11: 82,331,264 (GRCm39)	S407I	probably damaging	Het
Tmem50b	C	A	16: 91,377,180 (GRCm39)	A95S	possibly damaging	Het
Tnr	T	C	1: 159,719,603 (GRCm39)	I960T	probably benign	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tpcn1	G	C	5: 120,681,453 (GRCm39)	P532A	probably damaging	Het
Tpsb2	T	C	17: 25,585,539 (GRCm39)		probably benign	Het
Triobp	C	A	15: 78,888,740 (GRCm39)	H1948Q	probably damaging	Het
Tshb	T	C	3: 102,684,857 (GRCm39)	I116V	probably benign	Het
Ttc13	A	T	8: 125,398,950 (GRCm39)		probably null	Het
Usp34	A	T	11: 23,414,468 (GRCm39)	T2804S	probably damaging	Het
Vmn2r18	A	T	5: 151,486,016 (GRCm39)	C493S	possibly damaging	Het
Vmn2r2	T	C	3: 64,024,766 (GRCm39)	K605R	possibly damaging	Het
Vmn2r37	T	C	7: 9,220,792 (GRCm39)	Y357C	probably damaging	Het
Zc3h6	T	G	2: 128,857,538 (GRCm39)	S686A	possibly damaging	Het
Zfp608	G	A	18: 55,121,386 (GRCm39)	P67L	probably benign	Het
Zyg11a	T	C	4: 108,049,244 (GRCm39)		probably benign	Het

Other mutations in Fbxo43

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01445:Fbxo43	APN	15	36,151,972 (GRCm39)	missense	probably damaging	1.00
IGL02212:Fbxo43	APN	15	36,151,957 (GRCm39)	missense	probably damaging	0.99
IGL02246:Fbxo43	APN	15	36,162,842 (GRCm39)	missense	probably benign	0.06
IGL02576:Fbxo43	APN	15	36,152,321 (GRCm39)	missense	probably benign	0.01
FR4304:Fbxo43	UTSW	15	36,152,246 (GRCm39)	small insertion	probably benign
FR4304:Fbxo43	UTSW	15	36,152,243 (GRCm39)	small insertion	probably benign
FR4304:Fbxo43	UTSW	15	36,152,240 (GRCm39)	small insertion	probably benign
FR4548:Fbxo43	UTSW	15	36,152,244 (GRCm39)	nonsense	probably null
FR4589:Fbxo43	UTSW	15	36,152,247 (GRCm39)	small insertion	probably benign
FR4589:Fbxo43	UTSW	15	36,152,246 (GRCm39)	small insertion	probably benign
R0193:Fbxo43	UTSW	15	36,162,029 (GRCm39)	missense	probably benign	0.29
R0244:Fbxo43	UTSW	15	36,161,939 (GRCm39)	missense	probably damaging	1.00
R0322:Fbxo43	UTSW	15	36,152,338 (GRCm39)	splice site	probably benign
R0409:Fbxo43	UTSW	15	36,162,503 (GRCm39)	missense	probably benign	0.01
R0827:Fbxo43	UTSW	15	36,163,115 (GRCm39)	missense	possibly damaging	0.90
R1562:Fbxo43	UTSW	15	36,163,162 (GRCm39)	missense	probably damaging	0.99
R1880:Fbxo43	UTSW	15	36,162,661 (GRCm39)	missense	probably benign	0.02
R3792:Fbxo43	UTSW	15	36,163,005 (GRCm39)	missense	probably benign	0.03
R3875:Fbxo43	UTSW	15	36,162,249 (GRCm39)	missense	probably benign
R3876:Fbxo43	UTSW	15	36,152,258 (GRCm39)	missense	probably damaging	1.00
R5023:Fbxo43	UTSW	15	36,163,075 (GRCm39)	missense	probably benign	0.13
R5633:Fbxo43	UTSW	15	36,162,241 (GRCm39)	splice site	probably null
R5997:Fbxo43	UTSW	15	36,162,239 (GRCm39)	missense	probably damaging	1.00
R6589:Fbxo43	UTSW	15	36,162,686 (GRCm39)	missense	probably damaging	0.99
R7238:Fbxo43	UTSW	15	36,151,971 (GRCm39)	missense	probably damaging	1.00
R7536:Fbxo43	UTSW	15	36,161,997 (GRCm39)	missense	probably benign
R7689:Fbxo43	UTSW	15	36,163,201 (GRCm39)	missense	probably benign	0.00
R7780:Fbxo43	UTSW	15	36,162,358 (GRCm39)	missense	probably damaging	1.00
R8167:Fbxo43	UTSW	15	36,151,917 (GRCm39)	missense	probably damaging	1.00
R8184:Fbxo43	UTSW	15	36,162,485 (GRCm39)	missense	possibly damaging	0.53
R8306:Fbxo43	UTSW	15	36,162,013 (GRCm39)	missense	probably benign	0.01
R8393:Fbxo43	UTSW	15	36,162,494 (GRCm39)	missense	probably benign	0.06
R9099:Fbxo43	UTSW	15	36,162,619 (GRCm39)	missense	possibly damaging	0.56
R9658:Fbxo43	UTSW	15	36,152,282 (GRCm39)	missense	probably damaging	1.00
X0025:Fbxo43	UTSW	15	36,152,306 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAAAGCCAAGTTCCCACGCTTC -3'
(R):5'- AACCTATGGAGCAGATGTGAC -3'

Sequencing Primer
(F):5'- CTCCTCAGAGCCTTGCAGAC -3'
(R):5'- TGTGACTACATCTGTAACTCCAG -3'

Posted On

2014-09-17