Mutagenetix > Incidental Mutation

Incidental Mutation 'R2067:Myo1a'

226776

Institutional Source

Beutler Lab

Gene Symbol

Myo1a

Ensembl Gene

ENSMUSG00000025401

Gene Name

myosin IA

Synonyms

brush border myosin 1, BBM-I, Myhl

MMRRC Submission

040072-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

R2067 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

127705170-127720940 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 127705478 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 43 (N43D)

Ref Sequence

ENSEMBL: ENSMUSP00000078540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048099] [ENSMUST00000079590]

AlphaFold

O88329

Predicted Effect

probably benign
Transcript: ENSMUST00000048099

SMART Domains

Protein: ENSMUSP00000045988
Gene: ENSMUSG00000040195

Domain	Start	End	E-Value	Type
transmembrane domain	20	42	N/A	INTRINSIC
Pfam:DUF2215	150	396	1.2e-94	PFAM
low complexity region	416	426	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000079590
AA Change: N43D

PolyPhen 2 Score 0.247 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000078540
Gene: ENSMUSG00000025401
AA Change: N43D

Domain	Start	End	E-Value	Type
MYSc	3	695	N/A	SMART
IQ	696	718	1.27e-3	SMART
IQ	719	741	1.09e-2	SMART
IQ	742	764	7.52e-6	SMART
Pfam:Myosin_TH1	847	1035	1.4e-42	PFAM

Meta Mutation Damage Score

0.1798

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

97% (76/78)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional skeletal muscle myosin-1 (MYH1). Unconventional myosins contain the basic domains characteristic of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with autosomal dominant deafness. Alternatively spliced variants have been found for this gene. [provided by RefSeq, Dec 2011]
PHENOTYPE: Homozygous null mice display abnormal small intestine brush border morphology, but have normal hearing. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530002B09Rik	T	A	4: 122,689,322		probably benign	Het
Abca2	A	T	2: 25,437,505	I669F	possibly damaging	Het
Acin1	T	A	14: 54,665,254	Q360H	probably damaging	Het
Aldh3b1	T	A	19: 3,921,755	D72V	probably benign	Het
Alox12e	G	A	11: 70,316,002	R620W	probably damaging	Het
Alpl	T	C	4: 137,749,545		probably benign	Het
Amy2a1	T	C	3: 113,530,568	I108V	probably benign	Het
Ascc2	A	T	11: 4,681,496	M646L	probably benign	Het
Bod1l	G	A	5: 41,817,086	T2295M	probably benign	Het
Ccdc9	A	T	7: 16,278,550		probably null	Het
Clptm1l	C	T	13: 73,607,723	Q153*	probably null	Het
Csmd1	A	G	8: 15,900,782	S3476P	probably benign	Het
Ddias	A	T	7: 92,859,699	M336K	possibly damaging	Het
Ehd1	T	C	19: 6,298,078	L362P	probably benign	Het
Epha1	G	T	6: 42,366,053	H187Q	probably benign	Het
Espl1	T	C	15: 102,299,090	S330P	probably damaging	Het
Fbln7	G	T	2: 128,877,466	R61L	probably damaging	Het
Fbxo41	C	T	6: 85,478,471	W577*	probably null	Het
Fgb	C	A	3: 83,049,689	D25Y	probably benign	Het
Gc	T	A	5: 89,446,517	K37N	probably damaging	Het
Gfpt1	T	C	6: 87,057,754	I178T	probably benign	Het
Gm12695	T	C	4: 96,769,726	T69A	probably benign	Het
Gm3944	C	A	12: 18,853,894	S8*	probably null	Het
Gm9912	T	C	3: 149,185,159	T113A	unknown	Het
Gpr156	A	G	16: 37,978,751	D109G	probably benign	Het
Hoxd1	A	T	2: 74,763,366	T89S	probably benign	Het
Htt	C	T	5: 34,825,982	T975I	probably benign	Het
Itpr3	G	A	17: 27,098,076	M768I	probably benign	Het
Krt4	C	A	15: 101,924,664	A3S	possibly damaging	Het
Mrto4	T	C	4: 139,349,023	K86E	probably benign	Het
Mup4	T	A	4: 59,960,622		probably benign	Het
Myh1	G	A	11: 67,214,620	D1079N	possibly damaging	Het
Napa	A	T	7: 16,115,278		probably benign	Het
Ndufa12	A	G	10: 94,220,707	D99G	probably damaging	Het
Neb	A	G	2: 52,284,263	I1528T	probably benign	Het
Nek1	T	C	8: 61,007,162	S41P	probably damaging	Het
Nolc1	C	T	19: 46,083,607	T612M	probably damaging	Het
Nsun5	T	G	5: 135,375,072	Y301D	probably damaging	Het
Oas1g	T	C	5: 120,885,883	E121G	probably damaging	Het
Olfr1085	G	T	2: 86,658,437	T7K	probably damaging	Het
Olfr1170	A	G	2: 88,224,474	V186A	possibly damaging	Het
Olfr324	A	G	11: 58,597,570	N58S	probably damaging	Het
Olfr397	A	G	11: 73,964,914	Y102C	probably damaging	Het
Olfr522	T	A	7: 140,162,909	I14F	possibly damaging	Het
Olfr910	T	A	9: 38,539,280	N128K	probably benign	Het
Osmr	T	C	15: 6,815,415	N957D	probably benign	Het
Parn	G	A	16: 13,603,069	S473L	probably damaging	Het
Phc2	T	C	4: 128,747,136	F672S	probably damaging	Het
Pik3c2b	T	C	1: 133,099,611	S1283P	probably damaging	Het
Pole2	G	A	12: 69,228,152	R5W	probably benign	Het
Prl7a2	T	A	13: 27,660,887	Y172F	probably damaging	Het
Ptprz1	A	G	6: 23,050,389		probably benign	Het
Rapgef3	C	A	15: 97,766,961	G7V	probably damaging	Het
Ripor1	T	A	8: 105,617,708	S491R	probably benign	Het
Rnf141	A	G	7: 110,821,365		probably benign	Het
Ryr3	C	T	2: 112,946,957	R285Q	probably damaging	Het
Sall4	T	C	2: 168,756,545	N125S	probably benign	Het
Schip1	A	G	3: 68,617,786	K360R	probably damaging	Het
Senp6	T	A	9: 80,089,869	V55E	probably benign	Het
Skint1	T	A	4: 112,025,533	V258D	probably benign	Het
Slc25a16	T	A	10: 62,932,751	H130Q	probably benign	Het
Styx	T	C	14: 45,373,563	V217A	probably benign	Het
Syne2	G	A	12: 75,888,342		probably null	Het
Tagap1	A	G	17: 6,956,860	S146P	probably benign	Het
Tatdn2	A	G	6: 113,704,142	K379E	probably benign	Het
Thrap3	T	C	4: 126,175,396	Y654C	possibly damaging	Het
Tle2	T	C	10: 81,580,551	L135P	probably damaging	Het
Tmc1	A	G	19: 20,824,309	F451S	possibly damaging	Het
Trpm7	G	A	2: 126,797,727	P1650S	probably damaging	Het
Ttll4	G	A	1: 74,680,382	R16H	possibly damaging	Het
Ttn	T	C	2: 76,714,373	N32795S	probably damaging	Het
Tubgcp6	T	C	15: 89,104,489	E803G	probably benign	Het
Ubr2	A	G	17: 46,963,145		probably null	Het
Ugt2b35	A	G	5: 87,001,553	D221G	probably damaging	Het
Unc45b	G	A	11: 82,911,689	A4T	probably benign	Het
Vmn1r70	A	T	7: 10,634,337	I251F	possibly damaging	Het
Vmn2r120	T	A	17: 57,524,553	H412L	possibly damaging	Het
Zfp59	A	G	7: 27,853,510	N129S	probably benign	Het
Zgrf1	T	C	3: 127,613,350	C1589R	probably damaging	Het

Other mutations in Myo1a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01529:Myo1a	APN	10	127720660	missense	probably benign	0.00
IGL01896:Myo1a	APN	10	127719904	missense	probably benign
IGL02073:Myo1a	APN	10	127710225	missense	probably damaging	0.98
IGL02380:Myo1a	APN	10	127714485	missense	probably benign	0.00
IGL02507:Myo1a	APN	10	127712609	missense	probably damaging	0.98
R0106:Myo1a	UTSW	10	127719880	missense	probably benign	0.02
R0326:Myo1a	UTSW	10	127716297	missense	probably benign	0.00
R0357:Myo1a	UTSW	10	127710902	missense	probably benign	0.02
R0485:Myo1a	UTSW	10	127719242	splice site	probably benign
R0676:Myo1a	UTSW	10	127719880	missense	probably benign	0.02
R0707:Myo1a	UTSW	10	127719863	unclassified	probably benign
R1241:Myo1a	UTSW	10	127719279	missense	probably benign	0.00
R1441:Myo1a	UTSW	10	127719279	missense	probably benign	0.00
R1458:Myo1a	UTSW	10	127719937	missense	probably benign
R1546:Myo1a	UTSW	10	127712624	missense	probably damaging	1.00
R1692:Myo1a	UTSW	10	127719334	splice site	probably null
R1871:Myo1a	UTSW	10	127719671	missense	probably benign
R2079:Myo1a	UTSW	10	127720613	missense	probably benign	0.00
R2151:Myo1a	UTSW	10	127720181	missense	probably benign	0.18
R2375:Myo1a	UTSW	10	127705290	missense	probably damaging	1.00
R3014:Myo1a	UTSW	10	127716345	missense	probably damaging	1.00
R3741:Myo1a	UTSW	10	127714898	missense	probably benign	0.19
R3812:Myo1a	UTSW	10	127707415	missense	possibly damaging	0.89
R4303:Myo1a	UTSW	10	127713733	missense	probably benign	0.10
R4306:Myo1a	UTSW	10	127714081	missense	probably benign
R4472:Myo1a	UTSW	10	127710458	missense	probably benign	0.06
R4599:Myo1a	UTSW	10	127720151	splice site	probably null
R4604:Myo1a	UTSW	10	127711138	missense	probably damaging	1.00
R4649:Myo1a	UTSW	10	127710217	missense	probably benign	0.05
R4747:Myo1a	UTSW	10	127714438	missense	probably damaging	1.00
R4755:Myo1a	UTSW	10	127715688	missense	probably damaging	1.00
R4972:Myo1a	UTSW	10	127716309	missense	probably benign	0.31
R5072:Myo1a	UTSW	10	127707419	critical splice donor site	probably null
R5073:Myo1a	UTSW	10	127707419	critical splice donor site	probably null
R5074:Myo1a	UTSW	10	127707419	critical splice donor site	probably null
R5386:Myo1a	UTSW	10	127705897	nonsense	probably null
R5592:Myo1a	UTSW	10	127714039	missense	probably damaging	1.00
R5619:Myo1a	UTSW	10	127718544	missense	probably benign	0.00
R6001:Myo1a	UTSW	10	127706925	critical splice donor site	probably null
R6374:Myo1a	UTSW	10	127707680	missense	probably damaging	1.00
R6577:Myo1a	UTSW	10	127715320	missense	possibly damaging	0.94
R6932:Myo1a	UTSW	10	127710458	missense	probably benign	0.06
R7310:Myo1a	UTSW	10	127705828	missense	probably damaging	0.98
R7395:Myo1a	UTSW	10	127710440	missense	probably damaging	0.98
R7429:Myo1a	UTSW	10	127706847	missense	probably damaging	1.00
R7430:Myo1a	UTSW	10	127706847	missense	probably damaging	1.00
R8464:Myo1a	UTSW	10	127718584	missense	probably benign	0.01
R8523:Myo1a	UTSW	10	127711158	missense	probably damaging	1.00
R8722:Myo1a	UTSW	10	127706838	missense	probably damaging	1.00
R8803:Myo1a	UTSW	10	127710987	missense	probably benign	0.19
R8815:Myo1a	UTSW	10	127710174	missense	probably benign	0.17
R8862:Myo1a	UTSW	10	127712784	missense	probably benign	0.02
R8913:Myo1a	UTSW	10	127705841	missense	probably benign	0.06
R8917:Myo1a	UTSW	10	127715665	missense	possibly damaging	0.88
R9020:Myo1a	UTSW	10	127714123	missense	probably benign	0.01
R9429:Myo1a	UTSW	10	127707378	missense	probably damaging	1.00
X0067:Myo1a	UTSW	10	127713745	missense	probably damaging	1.00
Z1177:Myo1a	UTSW	10	127706875	missense	probably benign	0.20
Z1177:Myo1a	UTSW	10	127706881	missense	possibly damaging	0.69

Predicted Primers

PCR Primer
(F):5'- AAGACCTCATCCTCCTGGAG -3'
(R):5'- TCTAGGAAGGATTCTCAGGGG -3'

Sequencing Primer
(F):5'- ATCCTCCTGGAGCCCCTAGATG -3'
(R):5'- AGCTCCTTTGGGGAAAGACC -3'

Posted On

2014-09-17