Mutagenetix > Incidental Mutation

Incidental Mutation 'R2072:Vmn2r15'

227216

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r15

Ensembl Gene

ENSMUSG00000091375

Gene Name

vomeronasal 2, receptor 15

Synonyms

EG211223

MMRRC Submission

040077-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.135) question?

Stock #

R2072 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109286269-109297556 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109286753 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 695 (M695K)

Ref Sequence

ENSEMBL: ENSMUSP00000128333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167133]

AlphaFold

L7N2A0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167133
AA Change: M695K

PolyPhen 2 Score 0.655 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000128333
Gene: ENSMUSG00000091375
AA Change: M695K

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	75	472	1e-29	PFAM
Pfam:NCD3G	514	568	5.8e-18	PFAM
Pfam:7tm_3	601	836	9.1e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	C	5: 63,898,737	R272P	possibly damaging	Het
9130011E15Rik	A	T	19: 45,965,381	I188K	probably damaging	Het
Ablim3	A	T	18: 61,857,088	D83E	possibly damaging	Het
Aco1	G	A	4: 40,183,605	G508S	probably damaging	Het
Adamts13	C	A	2: 27,005,425	T1176N	probably benign	Het
Adgre5	T	C	8: 83,727,804	T357A	probably benign	Het
Akap8l	C	T	17: 32,332,483	R511H	probably damaging	Het
Ankrd33	T	C	15: 101,119,636	V310A	probably benign	Het
Bnipl	C	T	3: 95,244,211	G232E	probably damaging	Het
Btbd17	A	T	11: 114,791,952		probably null	Het
Cacna1s	G	A	1: 136,079,504	V173I	probably benign	Het
Ccdc122	T	C	14: 77,068,951		probably null	Het
Ces1a	T	A	8: 93,048,075	N12Y	probably benign	Het
Chrdl1	T	C	X: 143,303,418	I231V	probably benign	Het
Ciita	C	T	16: 10,518,353	T958I	probably benign	Het
Cnot1	G	T	8: 95,739,833	T1592K	possibly damaging	Het
Dcaf15	T	C	8: 84,101,741	D240G	probably damaging	Het
Ddx58	A	T	4: 40,224,069		probably null	Het
Dlgap1	C	T	17: 70,662,770	R524C	probably damaging	Het
Dmd	G	C	X: 84,312,483	A2257P	probably benign	Het
Dsg1c	A	G	18: 20,275,252	M453V	probably benign	Het
Ednrb	G	T	14: 103,817,099	N432K	probably benign	Het
Fcgbp	G	A	7: 28,120,389	G2514S	probably damaging	Het
Fez1	A	G	9: 36,867,945	K306R	probably benign	Het
Fmo4	A	G	1: 162,809,887	V12A	probably benign	Het
Fpgt	T	C	3: 155,087,874	Y172C	probably damaging	Het
Fsip2	T	A	2: 83,008,815	F6976I	possibly damaging	Het
Galnt12	C	T	4: 47,108,477	R205*	probably null	Het
Grik5	A	T	7: 25,015,313	M752K	possibly damaging	Het
Herc2	A	G	7: 56,226,964	N4516S	probably damaging	Het
Ifrd2	A	T	9: 107,592,545	D439V	probably damaging	Het
Igsf3	A	G	3: 101,439,515	T609A	probably benign	Het
Kif5a	T	C	10: 127,245,369	D232G	probably damaging	Het
Lgi2	A	G	5: 52,538,505	S371P	probably damaging	Het
March3	A	T	18: 56,811,853	V56E	possibly damaging	Het
Mib2	T	C	4: 155,659,701	D168G	probably damaging	Het
Nhs	T	A	X: 161,842,721	H544L	probably damaging	Het
Nlrp2	A	G	7: 5,325,006	S683P	probably damaging	Het
Olfr1260	C	A	2: 89,978,213	T145K	probably benign	Het
Olfr1454	A	T	19: 13,063,680	M90L	probably benign	Het
Olfr527	T	C	7: 140,336,653	S264P	possibly damaging	Het
Onecut3	T	G	10: 80,495,014	L3V	unknown	Het
Otogl	C	T	10: 107,781,043	C1791Y	probably damaging	Het
Paip1	T	C	13: 119,430,262	V128A	possibly damaging	Het
Pcnx2	A	T	8: 125,761,742	C1688S	possibly damaging	Het
Pdzd2	A	G	15: 12,385,819	L955P	probably damaging	Het
Phlpp2	T	C	8: 109,928,492	S605P	possibly damaging	Het
Pkhd1l1	G	T	15: 44,558,639	A3102S	probably damaging	Het
Plxnb2	G	T	15: 89,158,451	R1545S	probably damaging	Het
Ppp4c	T	C	7: 126,787,348		probably null	Het
Prune1	C	T	3: 95,255,408	R318Q	probably benign	Het
Psg27	T	C	7: 18,560,417	D355G	probably damaging	Het
Psg27	A	G	7: 18,565,009	L129P	probably benign	Het
Psmc6	A	G	14: 45,329,866	K7E	possibly damaging	Het
Reln	A	T	5: 21,919,177	V2777E	probably damaging	Het
Scn11a	G	T	9: 119,811,208	A207E	possibly damaging	Het
Slc5a5	C	T	8: 70,892,439	G75R	possibly damaging	Het
Smarcd2	A	T	11: 106,265,307	L42*	probably null	Het
Smg1	T	C	7: 118,163,166		probably benign	Het
Smurf2	T	A	11: 106,841,769	Q335L	probably benign	Het
Sspo	A	T	6: 48,473,517	H2580L	probably benign	Het
Stk3	T	C	15: 34,959,049	M256V	possibly damaging	Het
Syt1	A	G	10: 108,583,972	I276T	probably damaging	Het
Syt10	C	T	15: 89,790,776	D456N	probably damaging	Het
Taar9	A	T	10: 24,108,979	C186S	probably damaging	Het
Tnrc6b	C	T	15: 80,882,965	P977L	possibly damaging	Het
Trp53bp2	A	G	1: 182,458,867	T1091A	probably benign	Het
Ttn	G	T	2: 76,937,776	T2947N	probably damaging	Het
Ube2q1	T	C	3: 89,779,571		probably null	Het
Ube3c	A	G	5: 29,635,640	E671G	probably benign	Het
Upf3a	A	G	8: 13,785,850	K56R	possibly damaging	Het
Vmn2r3	A	T	3: 64,275,072	M402K	possibly damaging	Het
Zfp354b	T	A	11: 50,922,452	R549*	probably null	Het
Zfp37	A	T	4: 62,191,708	M411K	probably damaging	Het
Zfp747	T	A	7: 127,373,970	T343S	possibly damaging	Het
Zfp853	T	A	5: 143,289,382	Q161L	unknown	Het

Other mutations in Vmn2r15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01335:Vmn2r15	APN	5	109286736	missense	possibly damaging	0.70
IGL01367:Vmn2r15	APN	5	109293209	missense	probably damaging	0.99
IGL01844:Vmn2r15	APN	5	109286269	makesense	probably null
IGL02190:Vmn2r15	APN	5	109293374	missense	probably damaging	1.00
IGL02754:Vmn2r15	APN	5	109293268	nonsense	probably null
IGL02797:Vmn2r15	APN	5	109297384	missense	probably benign	0.18
IGL03301:Vmn2r15	APN	5	109297355	critical splice donor site	probably null
IGL03407:Vmn2r15	APN	5	109286319	nonsense	probably null
BB001:Vmn2r15	UTSW	5	109286388	missense	probably damaging	1.00
BB011:Vmn2r15	UTSW	5	109286388	missense	probably damaging	1.00
PIT4445001:Vmn2r15	UTSW	5	109287142	missense	probably damaging	0.99
PIT4520001:Vmn2r15	UTSW	5	109287005	missense	probably damaging	1.00
R0038:Vmn2r15	UTSW	5	109293144	missense	possibly damaging	0.46
R0111:Vmn2r15	UTSW	5	109287156	missense	possibly damaging	0.56
R0379:Vmn2r15	UTSW	5	109286478	missense	probably damaging	1.00
R0427:Vmn2r15	UTSW	5	109287087	missense	probably damaging	1.00
R0639:Vmn2r15	UTSW	5	109293015	missense	probably benign	0.22
R0964:Vmn2r15	UTSW	5	109297535	missense	probably benign	0.34
R1147:Vmn2r15	UTSW	5	109293206	missense	probably damaging	1.00
R1147:Vmn2r15	UTSW	5	109293206	missense	probably damaging	1.00
R1232:Vmn2r15	UTSW	5	109293302	missense	probably benign	0.39
R1241:Vmn2r15	UTSW	5	109292904	missense	probably damaging	1.00
R1244:Vmn2r15	UTSW	5	109293226	nonsense	probably null
R1394:Vmn2r15	UTSW	5	109294148	missense	probably benign	0.44
R1395:Vmn2r15	UTSW	5	109294148	missense	probably benign	0.44
R1423:Vmn2r15	UTSW	5	109293227	missense	probably damaging	1.00
R1439:Vmn2r15	UTSW	5	109294087	missense	probably damaging	1.00
R1513:Vmn2r15	UTSW	5	109293329	missense	probably damaging	1.00
R1777:Vmn2r15	UTSW	5	109294270	missense	possibly damaging	0.79
R1844:Vmn2r15	UTSW	5	109286994	nonsense	probably null
R2074:Vmn2r15	UTSW	5	109286753	missense	possibly damaging	0.65
R2122:Vmn2r15	UTSW	5	109286456	missense	probably damaging	1.00
R2208:Vmn2r15	UTSW	5	109297443	missense	possibly damaging	0.64
R2268:Vmn2r15	UTSW	5	109293207	missense	probably benign	0.31
R2831:Vmn2r15	UTSW	5	109286592	missense	probably damaging	1.00
R3848:Vmn2r15	UTSW	5	109297446	missense	probably benign	0.00
R4058:Vmn2r15	UTSW	5	109293446	missense	probably damaging	0.99
R4615:Vmn2r15	UTSW	5	109293482	missense	possibly damaging	0.91
R4663:Vmn2r15	UTSW	5	109294074	missense	probably benign
R4681:Vmn2r15	UTSW	5	109286622	missense	probably damaging	0.97
R4751:Vmn2r15	UTSW	5	109286754	missense	probably benign	0.01
R5095:Vmn2r15	UTSW	5	109288451	critical splice acceptor site	probably null
R5300:Vmn2r15	UTSW	5	109294108	missense	probably damaging	0.99
R5309:Vmn2r15	UTSW	5	109293090	missense	probably damaging	0.99
R5335:Vmn2r15	UTSW	5	109286807	missense	probably damaging	0.99
R5421:Vmn2r15	UTSW	5	109286535	missense	probably damaging	1.00
R5805:Vmn2r15	UTSW	5	109286940	missense	possibly damaging	0.88
R6280:Vmn2r15	UTSW	5	109293425	missense	possibly damaging	0.65
R6324:Vmn2r15	UTSW	5	109286271	makesense	probably null
R6383:Vmn2r15	UTSW	5	109293226	nonsense	probably null
R6772:Vmn2r15	UTSW	5	109286372	missense	probably damaging	0.99
R6991:Vmn2r15	UTSW	5	109293314	missense	probably damaging	1.00
R7194:Vmn2r15	UTSW	5	109292783	missense	probably damaging	1.00
R7365:Vmn2r15	UTSW	5	109293239	missense	probably benign	0.19
R7365:Vmn2r15	UTSW	5	109297522	missense	probably benign	0.15
R7423:Vmn2r15	UTSW	5	109297528	missense	probably benign	0.00
R7552:Vmn2r15	UTSW	5	109292908	nonsense	probably null
R7619:Vmn2r15	UTSW	5	109288324	critical splice donor site	probably null
R7892:Vmn2r15	UTSW	5	109286351	missense	probably damaging	1.00
R7924:Vmn2r15	UTSW	5	109286388	missense	probably damaging	1.00
R8058:Vmn2r15	UTSW	5	109293090	missense	probably damaging	0.99
R8099:Vmn2r15	UTSW	5	109293319	missense	possibly damaging	0.58
R8189:Vmn2r15	UTSW	5	109286847	missense	probably benign	0.02
R8275:Vmn2r15	UTSW	5	109286284	missense	probably benign	0.08
R8277:Vmn2r15	UTSW	5	109293104	missense	probably benign	0.00
R8277:Vmn2r15	UTSW	5	109293444	missense	probably damaging	1.00
R8465:Vmn2r15	UTSW	5	109297436	missense	probably damaging	1.00
R8679:Vmn2r15	UTSW	5	109286913	missense	probably benign	0.31
R8682:Vmn2r15	UTSW	5	109294072	missense	probably damaging	0.98
R8809:Vmn2r15	UTSW	5	109287008	missense	probably benign	0.22
R9016:Vmn2r15	UTSW	5	109294243	missense	probably benign	0.01
R9372:Vmn2r15	UTSW	5	109294087	missense	possibly damaging	0.60
R9596:Vmn2r15	UTSW	5	109292925	missense	probably benign	0.01
R9619:Vmn2r15	UTSW	5	109292756	missense	possibly damaging	0.58
R9685:Vmn2r15	UTSW	5	109292732	missense	probably benign	0.05
R9716:Vmn2r15	UTSW	5	109297358	missense	possibly damaging	0.88
R9772:Vmn2r15	UTSW	5	109287057	missense	probably damaging	1.00
X0065:Vmn2r15	UTSW	5	109293308	missense	probably damaging	1.00
Z1187:Vmn2r15	UTSW	5	109286663	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGAAGGCAATGACTGAGCC -3'
(R):5'- CCTATTGTGAAGGCCAATAACC -3'

Sequencing Primer
(F):5'- CAATGACTGAGCCTTTGTTGC -3'
(R):5'- TTGTGAAGGCCAATAACCGCATTG -3'

Posted On

2014-09-17