Mutagenetix > Incidental Mutation

Incidental Mutation 'R2073:Zscan29'

227305

Institutional Source

Beutler Lab

Gene Symbol

Zscan29

Ensembl Gene

ENSMUSG00000050619

Gene Name

zinc finger SCAN domains 29

Synonyms

Zfp690

MMRRC Submission

040078-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.159) question?

Stock #

R2073 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

120988754-121001606 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 120991336 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 817 (C817*)

Ref Sequence

ENSEMBL: ENSMUSP00000125987 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028702] [ENSMUST00000066155] [ENSMUST00000079024] [ENSMUST00000110661] [ENSMUST00000110665] [ENSMUST00000119031] [ENSMUST00000146243] [ENSMUST00000163766]

AlphaFold

E9Q5B4

Predicted Effect

probably benign
Transcript: ENSMUST00000028702

SMART Domains

Protein: ENSMUSP00000028702
Gene: ENSMUSG00000027259

Domain	Start	End	E-Value	Type
Pfam:A_deaminase	1	276	1.8e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000066155

SMART Domains

Protein: ENSMUSP00000067133
Gene: ENSMUSG00000027259

Domain	Start	End	E-Value	Type
Pfam:A_deaminase	16	343	1.6e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000079024

SMART Domains

Protein: ENSMUSP00000078033
Gene: ENSMUSG00000050619

Domain	Start	End	E-Value	Type
SCAN	13	125	5.83e-70	SMART
Pfam:Myb_DNA-bind_4	238	323	3e-21	PFAM
Pfam:Myb_DNA-bind_4	399	484	4.1e-22	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000110661
AA Change: C782*

SMART Domains

Protein: ENSMUSP00000106289
Gene: ENSMUSG00000050619
AA Change: C782*

Domain	Start	End	E-Value	Type
SCAN	13	125	5.83e-70	SMART
Pfam:Myb_DNA-bind_4	238	323	5.4e-21	PFAM
Pfam:Myb_DNA-bind_4	399	484	7.4e-22	PFAM
low complexity region	518	532	N/A	INTRINSIC
ZnF_C2H2	665	687	2.99e-4	SMART
ZnF_C2H2	693	715	2.75e-3	SMART
ZnF_C2H2	721	743	8.02e-5	SMART
ZnF_C2H2	749	771	1.13e-4	SMART
ZnF_C2H2	777	799	1.18e-2	SMART
ZnF_C2H2	805	827	1.33e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000110665

SMART Domains

Protein: ENSMUSP00000106293
Gene: ENSMUSG00000027259

Domain	Start	End	E-Value	Type
Pfam:A_deaminase	2	236	4.3e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000119031

SMART Domains

Protein: ENSMUSP00000113052
Gene: ENSMUSG00000027259

Domain	Start	End	E-Value	Type
Pfam:A_deaminase	16	343	3e-44	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000146243

SMART Domains

Protein: ENSMUSP00000120997
Gene: ENSMUSG00000050619

Domain	Start	End	E-Value	Type
SCAN	13	118	4.23e-58	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000163766
AA Change: C817*

SMART Domains

Protein: ENSMUSP00000125987
Gene: ENSMUSG00000050619
AA Change: C817*

Domain	Start	End	E-Value	Type
SCAN	13	125	5.83e-70	SMART
Pfam:Myb_DNA-bind_4	238	323	5.9e-21	PFAM
Pfam:Myb_DNA-bind_4	434	519	1.3e-21	PFAM
low complexity region	553	567	N/A	INTRINSIC
ZnF_C2H2	700	722	2.99e-4	SMART
ZnF_C2H2	728	750	2.75e-3	SMART
ZnF_C2H2	756	778	8.02e-5	SMART
ZnF_C2H2	784	806	1.13e-4	SMART
ZnF_C2H2	812	834	1.18e-2	SMART
ZnF_C2H2	840	862	1.33e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152164

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156370

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 86 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057M21Rik	T	C	7: 130,959,242 (GRCm39)	R153G	probably benign	Het
5730596B20Rik	C	A	6: 52,155,962 (GRCm39)	Y9*	probably null	Het
Aco1	G	A	4: 40,183,605 (GRCm39)	G508S	probably damaging	Het
Acot3	A	G	12: 84,100,230 (GRCm39)	H2R	possibly damaging	Het
Adamts13	T	A	2: 26,896,326 (GRCm39)	C1240S	probably damaging	Het
Adra1b	T	C	11: 43,726,698 (GRCm39)	N73S	probably damaging	Het
Aebp2	G	A	6: 140,579,420 (GRCm39)	S219N	probably benign	Het
Akap8l	C	T	17: 32,551,457 (GRCm39)	R511H	probably damaging	Het
Ank	T	C	15: 27,565,108 (GRCm39)	S270P	probably benign	Het
Ankle1	G	A	8: 71,861,973 (GRCm39)	R492H	possibly damaging	Het
Ankub1	G	A	3: 57,599,713 (GRCm39)	H19Y	possibly damaging	Het
Anln	C	A	9: 22,244,464 (GRCm39)	W1083L	probably benign	Het
Apaf1	G	T	10: 90,867,556 (GRCm39)	S763*	probably null	Het
Apba3	A	G	10: 81,105,128 (GRCm39)	T134A	probably benign	Het
Armh3	A	T	19: 45,953,820 (GRCm39)	I188K	probably damaging	Het
Bod1l	C	T	5: 41,976,532 (GRCm39)	S1594N	probably benign	Het
C7	T	C	15: 5,019,910 (GRCm39)	M746V	probably benign	Het
Cdkn2a	T	A	4: 89,212,730 (GRCm39)	I11F	possibly damaging	Het
Cideb	T	C	14: 55,992,617 (GRCm39)	M100V	possibly damaging	Het
Cntnap5c	T	C	17: 58,612,547 (GRCm39)	L862P	possibly damaging	Het
Coch	A	T	12: 51,649,472 (GRCm39)	D261V	probably benign	Het
Cyp2ab1	A	G	16: 20,132,639 (GRCm39)	F220L	possibly damaging	Het
Ddx10	A	T	9: 53,151,805 (GRCm39)	D73E	probably benign	Het
Dgkg	A	G	16: 22,384,067 (GRCm39)	F462S	probably damaging	Het
Dlgap3	C	T	4: 127,089,159 (GRCm39)	H252Y	probably damaging	Het
Dmd	G	C	X: 83,356,089 (GRCm39)	A2257P	probably benign	Het
Dnaaf6rt	A	G	1: 31,262,077 (GRCm39)	S20G	probably benign	Het
Dnai4	G	A	4: 102,907,390 (GRCm39)	T632M	probably damaging	Het
Dtnb	A	G	12: 3,831,273 (GRCm39)	T658A	probably benign	Het
Duox2	T	A	2: 122,125,639 (GRCm39)	S323C	probably damaging	Het
Dync1h1	T	A	12: 110,581,026 (GRCm39)	I251N	probably damaging	Het
Eml5	A	G	12: 98,768,705 (GRCm39)	S1457P	probably damaging	Het
Fam114a1	T	A	5: 65,153,247 (GRCm39)		probably null	Het
Fcho1	A	T	8: 72,163,133 (GRCm39)	L632Q	probably damaging	Het
Gm12695	T	C	4: 96,612,182 (GRCm39)	Y527C	possibly damaging	Het
Gm16223	T	C	5: 42,371,942 (GRCm39)	C111R	unknown	Het
H2-Q4	T	A	17: 35,599,378 (GRCm39)	S154T	possibly damaging	Het
Hhat	A	G	1: 192,409,687 (GRCm39)	F125L	possibly damaging	Het
Ier5l	T	A	2: 30,363,068 (GRCm39)	D319V	probably damaging	Het
Kif5a	T	C	10: 127,081,238 (GRCm39)	D232G	probably damaging	Het
M1ap	A	G	6: 82,958,863 (GRCm39)	I165V	probably benign	Het
Map2k4	A	C	11: 65,584,282 (GRCm39)	F334V	probably damaging	Het
Mmp19	G	A	10: 128,630,848 (GRCm39)	R156H	probably damaging	Het
Mpped2	T	A	2: 106,575,147 (GRCm39)	Y77*	probably null	Het
Nhs	T	A	X: 160,625,717 (GRCm39)	H544L	probably damaging	Het
Nr4a1	C	A	15: 101,171,948 (GRCm39)	H541N	probably damaging	Het
Or4a74	A	G	2: 89,439,822 (GRCm39)	V208A	probably benign	Het
Or5b122	A	T	19: 13,562,965 (GRCm39)	Q99L	probably damaging	Het
Pak6	A	C	2: 118,519,332 (GRCm39)	N17T	probably damaging	Het
Pcnt	T	A	10: 76,216,214 (GRCm39)	T2225S	possibly damaging	Het
Pdzd2	A	G	15: 12,385,905 (GRCm39)	L955P	probably damaging	Het
Phf21a	A	G	2: 92,178,381 (GRCm39)	D357G	probably damaging	Het
Pkhd1l1	G	T	15: 44,422,035 (GRCm39)	A3102S	probably damaging	Het
Plch2	A	T	4: 155,074,366 (GRCm39)	L754Q	probably damaging	Het
Plekhd1	A	G	12: 80,768,066 (GRCm39)	N335D	probably benign	Het
Pole	C	A	5: 110,473,417 (GRCm39)	T1737N	probably damaging	Het
Potefam1	T	C	2: 111,030,763 (GRCm39)	E382G	probably damaging	Het
Pramel6	G	A	2: 87,339,088 (GRCm39)	S96N	probably damaging	Het
Prdm14	A	C	1: 13,195,954 (GRCm39)	Y36D	possibly damaging	Het
Psg27	T	C	7: 18,294,342 (GRCm39)	D355G	probably damaging	Het
Rfc1	A	T	5: 65,459,282 (GRCm39)	D225E	probably damaging	Het
Sec16a	A	G	2: 26,330,251 (GRCm39)	I588T	probably damaging	Het
Setd6	G	A	8: 96,443,416 (GRCm39)	V60M	probably damaging	Het
Sgk3	A	G	1: 9,961,649 (GRCm39)	I432V	probably benign	Het
Six2	A	G	17: 85,994,933 (GRCm39)	S150P	probably damaging	Het
Slc1a6	T	C	10: 78,635,964 (GRCm39)	V343A	possibly damaging	Het
Slc43a3	T	C	2: 84,774,956 (GRCm39)		probably null	Het
Smc3	A	G	19: 53,619,964 (GRCm39)	D620G	probably benign	Het
Smg6	C	G	11: 74,821,120 (GRCm39)	P464A	probably damaging	Het
Sox11	T	C	12: 27,392,278 (GRCm39)	T44A	possibly damaging	Het
Spata22	A	G	11: 73,227,052 (GRCm39)	R89G	possibly damaging	Het
Spmip6	A	G	4: 41,507,519 (GRCm39)		probably null	Het
Stk3	T	C	15: 34,959,195 (GRCm39)	M256V	possibly damaging	Het
Syne2	A	G	12: 76,062,353 (GRCm39)	D4226G	possibly damaging	Het
Tecpr2	T	C	12: 110,934,863 (GRCm39)	S1370P	possibly damaging	Het
Tek	A	G	4: 94,715,966 (GRCm39)	I463V	probably benign	Het
Trib1	T	A	15: 59,526,189 (GRCm39)	I253N	probably damaging	Het
Triobp	G	T	15: 78,858,095 (GRCm39)	G1232V	probably damaging	Het
Trpm6	G	A	19: 18,853,406 (GRCm39)	V1809M	probably damaging	Het
Tsc22d4	A	G	5: 137,760,749 (GRCm39)	K57E	possibly damaging	Het
Vmn2r109	T	A	17: 20,784,974 (GRCm39)	K15N	probably benign	Het
Wnt9a	A	G	11: 59,222,055 (GRCm39)	N318D	probably damaging	Het
Wwp1	A	G	4: 19,662,181 (GRCm39)	V138A	possibly damaging	Het
Zfp37	A	T	4: 62,109,945 (GRCm39)	M411K	probably damaging	Het
Zfp715	T	A	7: 42,960,544 (GRCm39)	T16S	probably benign	Het
Zfp932	A	G	5: 110,157,684 (GRCm39)	T461A	possibly damaging	Het

Other mutations in Zscan29

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01935:Zscan29	APN	2	121,000,538 (GRCm39)	missense	probably damaging	1.00
IGL01938:Zscan29	APN	2	120,996,690 (GRCm39)	missense	probably benign	0.16
IGL02220:Zscan29	APN	2	120,997,170 (GRCm39)	missense	probably damaging	0.99
IGL02370:Zscan29	APN	2	120,994,314 (GRCm39)	missense	probably benign	0.00
IGL02585:Zscan29	APN	2	120,994,357 (GRCm39)	nonsense	probably null
R0284:Zscan29	UTSW	2	120,997,214 (GRCm39)	unclassified	probably benign
R0842:Zscan29	UTSW	2	120,991,960 (GRCm39)	missense	possibly damaging	0.84
R1245:Zscan29	UTSW	2	120,996,984 (GRCm39)	missense	probably damaging	1.00
R1586:Zscan29	UTSW	2	120,991,641 (GRCm39)	missense	probably damaging	1.00
R1654:Zscan29	UTSW	2	120,995,260 (GRCm39)	missense	probably benign	0.06
R1958:Zscan29	UTSW	2	121,000,289 (GRCm39)	critical splice donor site	probably null
R2085:Zscan29	UTSW	2	121,000,427 (GRCm39)	nonsense	probably null
R2145:Zscan29	UTSW	2	121,000,587 (GRCm39)	missense	probably damaging	1.00
R2201:Zscan29	UTSW	2	120,999,883 (GRCm39)	missense	probably damaging	1.00
R2875:Zscan29	UTSW	2	120,994,581 (GRCm39)	missense	probably damaging	1.00
R2876:Zscan29	UTSW	2	120,994,581 (GRCm39)	missense	probably damaging	1.00
R3861:Zscan29	UTSW	2	120,991,212 (GRCm39)	missense	probably benign	0.01
R4244:Zscan29	UTSW	2	120,995,275 (GRCm39)	splice site	probably null
R4245:Zscan29	UTSW	2	120,995,275 (GRCm39)	splice site	probably null
R4447:Zscan29	UTSW	2	121,000,367 (GRCm39)	splice site	probably null
R4662:Zscan29	UTSW	2	120,997,096 (GRCm39)	missense	probably benign	0.26
R4757:Zscan29	UTSW	2	120,991,392 (GRCm39)	missense	possibly damaging	0.92
R4777:Zscan29	UTSW	2	120,999,805 (GRCm39)	missense	probably damaging	0.96
R4905:Zscan29	UTSW	2	120,991,864 (GRCm39)	missense	possibly damaging	0.53
R4970:Zscan29	UTSW	2	120,999,676 (GRCm39)	splice site	probably null
R5860:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R5861:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R5862:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R5916:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R5917:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R5918:Zscan29	UTSW	2	120,994,518 (GRCm39)	missense	probably damaging	1.00
R6335:Zscan29	UTSW	2	120,991,917 (GRCm39)	missense	possibly damaging	0.49
R7214:Zscan29	UTSW	2	120,999,761 (GRCm39)	nonsense	probably null
R7326:Zscan29	UTSW	2	120,991,469 (GRCm39)	missense	probably damaging	1.00
R7997:Zscan29	UTSW	2	120,991,221 (GRCm39)	missense	probably benign	0.01
R8787:Zscan29	UTSW	2	120,996,876 (GRCm39)	missense	probably damaging	1.00
RF001:Zscan29	UTSW	2	120,994,477 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- GCACTGACTATGACAGCAGC -3'
(R):5'- ACTCCCATCTCAGGTATCCG -3'

Sequencing Primer
(F):5'- GACTATGACAGCAGCTTTTCTCGAG -3'
(R):5'- AAAGGTTTCCGTGACAGTTCC -3'

Posted On

2014-09-17