Mutagenetix > Incidental Mutation

Incidental Mutation 'R2060:Unc80'

228532

Institutional Source

Beutler Lab

Gene Symbol

Unc80

Ensembl Gene

ENSMUSG00000055567

Gene Name

unc-80, NALCN activator

Synonyms

C030018G13Rik, C230061B10Rik

MMRRC Submission

040065-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.920) question?

Stock #

R2060 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66468367-66699148 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 66640595 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 2108 (H2108Q)

Ref Sequence

ENSEMBL: ENSMUSP00000053692 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061620] [ENSMUST00000212557]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061620
AA Change: H2108Q

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000053692
Gene: ENSMUSG00000055567
AA Change: H2108Q

Domain	Start	End	E-Value	Type
Pfam:UNC80	16	236	2.2e-94	PFAM
low complexity region	372	385	N/A	INTRINSIC
low complexity region	493	502	N/A	INTRINSIC
low complexity region	693	711	N/A	INTRINSIC
low complexity region	723	738	N/A	INTRINSIC
low complexity region	739	769	N/A	INTRINSIC
low complexity region	1038	1055	N/A	INTRINSIC
low complexity region	1067	1084	N/A	INTRINSIC
low complexity region	1309	1328	N/A	INTRINSIC
low complexity region	1477	1489	N/A	INTRINSIC
low complexity region	1676	1681	N/A	INTRINSIC
low complexity region	1842	1848	N/A	INTRINSIC
low complexity region	1854	1868	N/A	INTRINSIC
low complexity region	2461	2480	N/A	INTRINSIC
low complexity region	2726	2740	N/A	INTRINSIC
low complexity region	3121	3144	N/A	INTRINSIC
low complexity region	3245	3254	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000212557
AA Change: H2040Q

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of a voltage-independent 'leak' ion-channel complex, in which it performs essential functions, such as serving as a bridge between two other components (sodium leak channel non-selective and UNC79) and as a scaffold for Src kinases. Leak channels play an importnat role in establishment and maintenance of resting membrane potentials in neurons. Mutations in this gene are associated with congenital infantile encephalopathy, intellectual disability and growth issues. [provided by RefSeq, Aug 2016]

Allele List at MGI

Other mutations in this stock

Total: 129 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
8430408G22Rik	G	A	6: 116,651,722 (GRCm38)	V9M	possibly damaging	Het
Abca14	G	A	7: 120,227,518 (GRCm38)	W462*	probably null	Het
Aftph	A	T	11: 20,692,571 (GRCm38)	Y821N	probably damaging	Het
Ahnak	A	T	19: 9,008,041 (GRCm38)	M2230L	probably benign	Het
Arfgap1	T	C	2: 180,972,782 (GRCm38)	F144L	probably benign	Het
Arid4b	C	T	13: 14,195,452 (GRCm38)	R1178C	probably damaging	Het
Asb8	A	T	15: 98,141,373 (GRCm38)	C49S	possibly damaging	Het
Baz1b	A	G	5: 135,205,114 (GRCm38)	N165S	probably damaging	Het
BC017158	A	G	7: 128,288,331 (GRCm38)	L176P	probably damaging	Het
Bod1l	A	T	5: 41,808,742 (GRCm38)	I2660N	possibly damaging	Het
C2cd3	T	C	7: 100,454,948 (GRCm38)	I825T	probably damaging	Het
C4b	A	G	17: 34,736,101 (GRCm38)	W804R	probably damaging	Het
Cadm3	A	T	1: 173,344,402 (GRCm38)	D201E	probably damaging	Het
Cdh17	T	C	4: 11,803,982 (GRCm38)	F552L	probably benign	Het
Cdh7	G	C	1: 110,048,877 (GRCm38)	A91P	probably damaging	Het
Cela1	A	G	15: 100,675,322 (GRCm38)		probably null	Het
Clk3	T	C	9: 57,751,117 (GRCm38)	Y582C	probably damaging	Het
Cma1	C	T	14: 55,943,698 (GRCm38)		probably null	Het
Ctcfl	A	G	2: 173,118,506 (GRCm38)	S95P	probably benign	Het
Cylc1	C	A	X: 111,123,123 (GRCm38)	T391K	unknown	Het
Cyp3a11	T	A	5: 145,855,081 (GRCm38)	I501L	probably benign	Het
Cyp3a59	T	A	5: 146,104,714 (GRCm38)	L356Q	probably damaging	Het
Dcdc2a	A	C	13: 25,107,710 (GRCm38)	D226A	possibly damaging	Het
Dlec1	A	C	9: 119,112,086 (GRCm38)	T235P	probably damaging	Het
Dnaaf1	G	A	8: 119,590,602 (GRCm38)	R290Q	probably benign	Het
Dnaaf5	C	T	5: 139,178,003 (GRCm38)	R377W	probably damaging	Het
Dpep1	T	A	8: 123,200,391 (GRCm38)	V293E	probably damaging	Het
Drosha	T	A	15: 12,924,159 (GRCm38)	V1209E	possibly damaging	Het
Dync2h1	T	G	9: 7,162,802 (GRCm38)	I596L	possibly damaging	Het
Edem1	T	A	6: 108,854,287 (GRCm38)	Y570N	probably damaging	Het
Edrf1	G	T	7: 133,657,129 (GRCm38)	E9*	probably null	Het
Enpep	T	A	3: 129,280,523 (GRCm38)	N792Y	probably benign	Het
Enpp2	A	T	15: 54,875,714 (GRCm38)	M391K	probably damaging	Het
Fanca	A	C	8: 123,274,481 (GRCm38)	V1105G	probably damaging	Het
Fbxo22	T	A	9: 55,218,383 (GRCm38)	L74I	probably damaging	Het
Fchsd2	T	A	7: 101,277,417 (GRCm38)	F571L	probably benign	Het
Fhad1	T	C	4: 141,899,249 (GRCm38)	D1345G	probably benign	Het
G2e3	T	C	12: 51,372,606 (GRCm38)	F702L	probably damaging	Het
Glce	A	T	9: 62,060,946 (GRCm38)	S308T	possibly damaging	Het
Glt1d1	A	G	5: 127,657,119 (GRCm38)	D119G	probably benign	Het
Gpr137c	T	C	14: 45,244,159 (GRCm38)	I144T	probably damaging	Het
Gprin3	A	G	6: 59,354,519 (GRCm38)	C268R	possibly damaging	Het
Hadha	G	A	5: 30,128,836 (GRCm38)	T395M	probably benign	Het
Hdhd2	T	G	18: 76,965,042 (GRCm38)		probably null	Het
Homer2	C	T	7: 81,618,703 (GRCm38)	E70K	probably benign	Het
Hp1bp3	T	A	4: 138,240,672 (GRCm38)	D397E	probably damaging	Het
Hrh3	T	C	2: 180,101,250 (GRCm38)	N195S	possibly damaging	Het
Hyou1	T	C	9: 44,381,552 (GRCm38)	V153A	probably benign	Het
Igf2r	A	T	17: 12,701,319 (GRCm38)	S1378T	possibly damaging	Het
Ints4	G	A	7: 97,501,763 (GRCm38)	R279H	possibly damaging	Het
Itga10	A	T	3: 96,654,998 (GRCm38)	R699*	probably null	Het
Itpkb	A	G	1: 180,421,858 (GRCm38)	T933A	probably benign	Het
Itsn2	T	G	12: 4,627,879 (GRCm38)	F79V	probably damaging	Het
Jak3	A	T	8: 71,683,415 (GRCm38)	K620*	probably null	Het
Jak3	C	A	8: 71,680,714 (GRCm38)	C350*	probably null	Het
Kcnq5	T	C	1: 21,461,597 (GRCm38)	S421G	probably benign	Het
Kdm2b	A	T	5: 122,883,365 (GRCm38)	M50K	probably damaging	Het
Klk1b1	A	G	7: 43,970,623 (GRCm38)	D170G	possibly damaging	Het
Lama3	A	T	18: 12,528,726 (GRCm38)	T2160S	probably benign	Het
Lman1	A	T	18: 65,998,352 (GRCm38)		probably benign	Het
Lmtk3	G	A	7: 45,800,911 (GRCm38)		probably null	Het
Ltb	A	G	17: 35,195,763 (GRCm38)	R180G	probably damaging	Het
Ltbp4	C	T	7: 27,308,953 (GRCm38)	R1310Q	probably damaging	Het
Macf1	T	A	4: 123,499,919 (GRCm38)		probably null	Het
Mast4	G	T	13: 102,738,846 (GRCm38)	P1146Q	probably damaging	Het
Micall2	C	T	5: 139,711,562 (GRCm38)	S678N	probably damaging	Het
Mon2	A	T	10: 122,995,776 (GRCm38)	I1675N	probably damaging	Het
Mug2	A	G	6: 122,079,612 (GRCm38)	N1172S	probably benign	Het
Naa30	C	G	14: 49,173,099 (GRCm38)	S161R	possibly damaging	Het
Ncaph	T	C	2: 127,124,875 (GRCm38)	N220D	probably damaging	Het
Nell1	T	C	7: 50,560,830 (GRCm38)	V497A	possibly damaging	Het
Ngly1	A	G	14: 16,277,877 (GRCm38)	N142S	possibly damaging	Het
Nin	T	A	12: 70,042,418 (GRCm38)	T1408S	possibly damaging	Het
Nlrp4g	T	A	9: 124,349,693 (GRCm38)		noncoding transcript	Het
Nrg1	T	G	8: 31,918,015 (GRCm38)	E63D	probably damaging	Het
Ntn4	C	T	10: 93,707,353 (GRCm38)	R314W	probably damaging	Het
Olfr1167	T	C	2: 88,149,143 (GRCm38)	Y292C	probably damaging	Het
Olfr128	A	G	17: 37,923,880 (GRCm38)	T105A	probably benign	Het
Olfr473	C	T	7: 107,933,661 (GRCm38)	T47M	probably benign	Het
Olfr522	T	A	7: 140,162,824 (GRCm38)	E42V	probably damaging	Het
Olfr845	A	G	9: 19,339,056 (GRCm38)	I199V	probably benign	Het
Olfr851	T	A	9: 19,497,237 (GRCm38)	V163E	possibly damaging	Het
Olfr867	A	T	9: 20,054,596 (GRCm38)	I289N	probably damaging	Het
Olfr998	G	A	2: 85,591,283 (GRCm38)	V248I	possibly damaging	Het
Orc5	C	T	5: 22,516,703 (GRCm38)		probably null	Het
Pard3	G	A	8: 127,398,604 (GRCm38)	R691Q	probably benign	Het
Pofut1	T	A	2: 153,243,660 (GRCm38)	D54E	probably benign	Het
Prl2c5	T	A	13: 13,190,653 (GRCm38)	V128E	probably damaging	Het
Ptk7	T	C	17: 46,566,238 (GRCm38)	M965V	possibly damaging	Het
Pum2	C	T	12: 8,728,726 (GRCm38)	R459*	probably null	Het
Pzp	A	T	6: 128,483,710 (GRCm38)	N1494K	probably benign	Het
Rad21l	A	G	2: 151,645,429 (GRCm38)	V545A	probably benign	Het
Rps6kc1	A	T	1: 190,810,108 (GRCm38)	M352K	possibly damaging	Het
Rpusd2	G	A	2: 119,037,215 (GRCm38)		probably null	Het
Rsph14	A	T	10: 75,029,771 (GRCm38)	D78E	probably damaging	Het
Rtl9	A	T	X: 143,102,030 (GRCm38)	M813L	possibly damaging	Het
Rtp4	A	T	16: 23,612,940 (GRCm38)	H74L	probably damaging	Het
Rusc1	G	A	3: 89,087,848 (GRCm38)	T725I	possibly damaging	Het
Ryr2	C	T	13: 11,595,736 (GRCm38)	C4068Y	probably damaging	Het
Ryr3	A	G	2: 112,954,364 (GRCm38)	V224A	possibly damaging	Het
Shprh	A	T	10: 11,152,120 (GRCm38)	N157I	probably benign	Het
Siglece	A	G	7: 43,657,786 (GRCm38)	I67T	probably benign	Het
Slc9b2	A	T	3: 135,326,266 (GRCm38)	T296S	probably damaging	Het
Sorbs2	A	G	8: 45,775,629 (GRCm38)	K276E	probably damaging	Het
Synj2	A	G	17: 6,037,480 (GRCm38)	T1269A	probably benign	Het
Taar7b	T	C	10: 24,000,675 (GRCm38)	I246T	possibly damaging	Het
Taldo1	A	G	7: 141,396,154 (GRCm38)	Y113C	probably damaging	Het
Tarbp1	A	T	8: 126,447,594 (GRCm38)		probably null	Het
Tars	C	T	15: 11,394,373 (GRCm38)	M59I	probably benign	Het
Tas2r130	G	A	6: 131,630,817 (GRCm38)	T5I	probably benign	Het
Tex48	T	C	4: 63,607,415 (GRCm38)	E77G	probably damaging	Het
Tmco5	A	G	2: 116,892,255 (GRCm38)	R286G	probably damaging	Het
Trdmt1	A	T	2: 13,519,914 (GRCm38)	H243Q	probably benign	Het
Ttn	G	A	2: 76,897,580 (GRCm38)		probably benign	Het
Ttn	T	A	2: 76,734,294 (GRCm38)	R26754*	probably null	Het
Ubqln3	A	C	7: 104,142,151 (GRCm38)	L244R	probably damaging	Het
Ubxn1	C	T	19: 8,873,566 (GRCm38)	R115*	probably null	Het
Umod	T	G	7: 119,476,715 (GRCm38)	N276T	probably damaging	Het
Unc13c	A	T	9: 73,665,656 (GRCm38)	L1528Q	probably damaging	Het
Utp20	A	C	10: 88,774,795 (GRCm38)	D1442E	probably damaging	Het
Utp4	A	G	8: 106,898,521 (GRCm38)	Q144R	probably benign	Het
Vmn2r17	T	A	5: 109,427,209 (GRCm38)	N127K	probably benign	Het
Vmn2r75	T	A	7: 86,165,164 (GRCm38)	T374S	probably benign	Het
Washc5	A	G	15: 59,350,408 (GRCm38)	F523L	probably damaging	Het
Wdr3	A	T	3: 100,159,897 (GRCm38)		probably null	Het
Wdr89	T	C	12: 75,632,988 (GRCm38)	Y164C	probably damaging	Het
Xpo5	T	C	17: 46,225,091 (GRCm38)	S550P	probably damaging	Het
Zfp69	T	C	4: 120,930,832 (GRCm38)	T429A	probably damaging	Het
Zfpm1	G	T	8: 122,336,592 (GRCm38)	G797C	probably benign	Het

Other mutations in Unc80

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00160:Unc80	APN	1	66,654,395 (GRCm38)	missense	possibly damaging	0.53
IGL00340:Unc80	APN	1	66,606,459 (GRCm38)	missense	possibly damaging	0.73
IGL00783:Unc80	APN	1	66,608,437 (GRCm38)	missense	probably benign	0.37
IGL00784:Unc80	APN	1	66,608,437 (GRCm38)	missense	probably benign	0.37
IGL00935:Unc80	APN	1	66,627,266 (GRCm38)	missense	possibly damaging	0.53
IGL01094:Unc80	APN	1	66,695,433 (GRCm38)	missense	possibly damaging	0.90
IGL01466:Unc80	APN	1	66,622,486 (GRCm38)	missense	probably benign	0.33
IGL01577:Unc80	APN	1	66,529,968 (GRCm38)	splice site	probably null
IGL01626:Unc80	APN	1	66,551,054 (GRCm38)	critical splice donor site	probably null
IGL01640:Unc80	APN	1	66,679,585 (GRCm38)	missense	probably benign	0.33
IGL01775:Unc80	APN	1	66,601,056 (GRCm38)	missense	possibly damaging	0.94
IGL01960:Unc80	APN	1	66,608,500 (GRCm38)	splice site	probably benign
IGL01991:Unc80	APN	1	66,469,509 (GRCm38)	nonsense	probably null
IGL02022:Unc80	APN	1	66,626,516 (GRCm38)	missense	possibly damaging	0.53
IGL02073:Unc80	APN	1	66,612,227 (GRCm38)	missense	possibly damaging	0.85
IGL02077:Unc80	APN	1	66,525,716 (GRCm38)	missense	possibly damaging	0.77
IGL02197:Unc80	APN	1	66,530,065 (GRCm38)	missense	probably benign	0.39
IGL02198:Unc80	APN	1	66,529,986 (GRCm38)	missense	possibly damaging	0.88
IGL02228:Unc80	APN	1	66,608,428 (GRCm38)	missense	possibly damaging	0.72
IGL02327:Unc80	APN	1	66,641,673 (GRCm38)	missense	probably benign	0.33
IGL02447:Unc80	APN	1	66,503,544 (GRCm38)	missense	possibly damaging	0.86
IGL02489:Unc80	APN	1	66,525,701 (GRCm38)	missense	probably benign	0.07
IGL02546:Unc80	APN	1	66,554,953 (GRCm38)	missense	possibly damaging	0.83
IGL02629:Unc80	APN	1	66,483,317 (GRCm38)	missense	possibly damaging	0.46
IGL02631:Unc80	APN	1	66,530,063 (GRCm38)	missense	probably damaging	0.98
IGL02839:Unc80	APN	1	66,671,675 (GRCm38)	missense	possibly damaging	0.53
IGL02960:Unc80	APN	1	66,678,058 (GRCm38)	splice site	probably benign
IGL02974:Unc80	APN	1	66,525,658 (GRCm38)	missense	possibly damaging	0.95
IGL03060:Unc80	APN	1	66,637,010 (GRCm38)	missense	possibly damaging	0.96
IGL03062:Unc80	APN	1	66,509,489 (GRCm38)	missense	probably damaging	0.96
IGL03074:Unc80	APN	1	66,671,718 (GRCm38)	splice site	probably benign
IGL03086:Unc80	APN	1	66,509,474 (GRCm38)	missense	probably damaging	0.99
IGL03105:Unc80	APN	1	66,472,099 (GRCm38)	missense	probably damaging	0.96
IGL03107:Unc80	APN	1	66,631,454 (GRCm38)	missense	probably damaging	0.98
IGL03158:Unc80	APN	1	66,641,674 (GRCm38)	missense	probably benign	0.33
IGL03220:Unc80	APN	1	66,504,938 (GRCm38)	missense	probably damaging	0.99
IGL03271:Unc80	APN	1	66,695,603 (GRCm38)	unclassified	probably benign
IGL03332:Unc80	APN	1	66,503,631 (GRCm38)	missense	probably damaging	1.00
IGL03347:Unc80	APN	1	66,695,466 (GRCm38)	missense	probably damaging	1.00
R0012:Unc80	UTSW	1	66,507,391 (GRCm38)	missense	probably damaging	1.00
R0012:Unc80	UTSW	1	66,507,391 (GRCm38)	missense	probably damaging	1.00
R0026:Unc80	UTSW	1	66,521,584 (GRCm38)	missense	probably benign	0.27
R0055:Unc80	UTSW	1	66,506,623 (GRCm38)	splice site	probably benign
R0149:Unc80	UTSW	1	66,521,601 (GRCm38)	missense	possibly damaging	0.82
R0325:Unc80	UTSW	1	66,510,881 (GRCm38)	missense	probably damaging	1.00
R0329:Unc80	UTSW	1	66,674,087 (GRCm38)	missense	possibly damaging	0.96
R0330:Unc80	UTSW	1	66,674,087 (GRCm38)	missense	possibly damaging	0.96
R0355:Unc80	UTSW	1	66,549,856 (GRCm38)	missense	possibly damaging	0.77
R0412:Unc80	UTSW	1	66,550,937 (GRCm38)	splice site	probably benign
R0422:Unc80	UTSW	1	66,483,338 (GRCm38)	missense	probably damaging	1.00
R0477:Unc80	UTSW	1	66,570,001 (GRCm38)	missense	probably damaging	0.99
R0507:Unc80	UTSW	1	66,527,893 (GRCm38)	missense	possibly damaging	0.66
R0513:Unc80	UTSW	1	66,622,474 (GRCm38)	missense	possibly damaging	0.73
R0553:Unc80	UTSW	1	66,506,669 (GRCm38)	missense	probably damaging	0.97
R0626:Unc80	UTSW	1	66,608,442 (GRCm38)	missense	probably benign	0.01
R0655:Unc80	UTSW	1	66,503,781 (GRCm38)	missense	probably damaging	0.98
R0742:Unc80	UTSW	1	66,527,893 (GRCm38)	missense	possibly damaging	0.66
R0755:Unc80	UTSW	1	66,504,923 (GRCm38)	missense	probably damaging	1.00
R0782:Unc80	UTSW	1	66,622,581 (GRCm38)	missense	possibly damaging	0.53
R0837:Unc80	UTSW	1	66,648,944 (GRCm38)	missense	possibly damaging	0.73
R0841:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R0893:Unc80	UTSW	1	66,521,486 (GRCm38)	missense	probably damaging	0.97
R0900:Unc80	UTSW	1	66,671,598 (GRCm38)	missense	probably benign	0.33
R0924:Unc80	UTSW	1	66,510,641 (GRCm38)	missense	possibly damaging	0.95
R0930:Unc80	UTSW	1	66,510,641 (GRCm38)	missense	possibly damaging	0.95
R0989:Unc80	UTSW	1	66,646,440 (GRCm38)	missense	possibly damaging	0.53
R1145:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R1145:Unc80	UTSW	1	66,472,088 (GRCm38)	missense	probably damaging	1.00
R1224:Unc80	UTSW	1	66,471,980 (GRCm38)	missense	probably damaging	1.00
R1240:Unc80	UTSW	1	66,635,902 (GRCm38)	missense	possibly damaging	0.85
R1245:Unc80	UTSW	1	66,555,095 (GRCm38)	missense	possibly damaging	0.94
R1467:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1473:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1500:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1556:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R1562:Unc80	UTSW	1	66,637,957 (GRCm38)	missense	probably damaging	1.00
R1655:Unc80	UTSW	1	66,672,756 (GRCm38)	missense	possibly damaging	0.86
R1674:Unc80	UTSW	1	66,509,308 (GRCm38)	missense	probably damaging	1.00
R1680:Unc80	UTSW	1	66,503,669 (GRCm38)	nonsense	probably null
R1739:Unc80	UTSW	1	66,527,892 (GRCm38)	missense	probably damaging	0.97
R1756:Unc80	UTSW	1	66,639,248 (GRCm38)	missense	possibly damaging	0.53
R1783:Unc80	UTSW	1	66,683,273 (GRCm38)	missense	probably benign	0.01
R1834:Unc80	UTSW	1	66,639,248 (GRCm38)	missense	possibly damaging	0.53
R1854:Unc80	UTSW	1	66,631,414 (GRCm38)	missense	possibly damaging	0.93
R1871:Unc80	UTSW	1	66,510,717 (GRCm38)	missense	possibly damaging	0.77
R1878:Unc80	UTSW	1	66,509,402 (GRCm38)	missense	probably damaging	0.96
R1883:Unc80	UTSW	1	66,525,770 (GRCm38)	missense	possibly damaging	0.89
R1912:Unc80	UTSW	1	66,510,625 (GRCm38)	missense	probably damaging	1.00
R1990:Unc80	UTSW	1	66,692,549 (GRCm38)	missense	probably damaging	0.97
R2007:Unc80	UTSW	1	66,503,776 (GRCm38)	missense	probably damaging	1.00
R2035:Unc80	UTSW	1	66,606,593 (GRCm38)	missense	probably damaging	0.98
R2056:Unc80	UTSW	1	66,640,552 (GRCm38)	missense	possibly damaging	0.72
R2074:Unc80	UTSW	1	66,679,744 (GRCm38)	critical splice donor site	probably null
R2088:Unc80	UTSW	1	66,590,227 (GRCm38)	missense	possibly damaging	0.77
R2089:Unc80	UTSW	1	66,671,715 (GRCm38)	splice site	probably benign
R2091:Unc80	UTSW	1	66,671,715 (GRCm38)	splice site	probably benign
R2139:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2169:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2175:Unc80	UTSW	1	66,677,355 (GRCm38)	missense	probably damaging	1.00
R2248:Unc80	UTSW	1	66,623,206 (GRCm38)	splice site	probably benign
R2255:Unc80	UTSW	1	66,618,258 (GRCm38)	missense	possibly damaging	0.53
R2308:Unc80	UTSW	1	66,648,997 (GRCm38)	missense	possibly damaging	0.53
R2484:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
R2507:Unc80	UTSW	1	66,612,107 (GRCm38)	missense	possibly damaging	0.53
R2512:Unc80	UTSW	1	66,671,608 (GRCm38)	missense	possibly damaging	0.70
R2878:Unc80	UTSW	1	66,671,576 (GRCm38)	critical splice acceptor site	probably benign
R3040:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3104:Unc80	UTSW	1	66,623,291 (GRCm38)	missense	probably benign	0.33
R3402:Unc80	UTSW	1	66,510,686 (GRCm38)	missense	probably damaging	0.97
R3403:Unc80	UTSW	1	66,510,686 (GRCm38)	missense	probably damaging	0.97
R3413:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3426:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3427:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3428:Unc80	UTSW	1	66,639,305 (GRCm38)	missense	probably benign	0.33
R3904:Unc80	UTSW	1	66,639,296 (GRCm38)	nonsense	probably null
R3916:Unc80	UTSW	1	66,677,495 (GRCm38)	missense	probably benign	0.11
R3950:Unc80	UTSW	1	66,622,570 (GRCm38)	missense	possibly damaging	0.53
R4642:Unc80	UTSW	1	66,671,714 (GRCm38)	splice site	probably null
R4646:Unc80	UTSW	1	66,669,235 (GRCm38)	missense	probably benign	0.03
R4655:Unc80	UTSW	1	66,671,662 (GRCm38)	missense	probably benign	0.18
R4662:Unc80	UTSW	1	66,646,436 (GRCm38)	missense	probably benign	0.01
R4720:Unc80	UTSW	1	66,510,792 (GRCm38)	missense	possibly damaging	0.92
R4736:Unc80	UTSW	1	66,649,672 (GRCm38)	critical splice acceptor site	probably null
R4795:Unc80	UTSW	1	66,527,941 (GRCm38)	missense	probably damaging	0.97
R4888:Unc80	UTSW	1	66,644,447 (GRCm38)	missense	probably damaging	0.98
R4917:Unc80	UTSW	1	66,646,550 (GRCm38)	missense	possibly damaging	0.86
R4918:Unc80	UTSW	1	66,646,550 (GRCm38)	missense	possibly damaging	0.86
R4983:Unc80	UTSW	1	66,674,732 (GRCm38)	splice site	probably null
R5051:Unc80	UTSW	1	66,509,477 (GRCm38)	missense	probably damaging	0.96
R5111:Unc80	UTSW	1	66,527,995 (GRCm38)	missense	possibly damaging	0.66
R5122:Unc80	UTSW	1	66,679,590 (GRCm38)	missense	possibly damaging	0.53
R5260:Unc80	UTSW	1	66,646,587 (GRCm38)	missense	possibly damaging	0.53
R5351:Unc80	UTSW	1	66,606,513 (GRCm38)	missense	possibly damaging	0.73
R5387:Unc80	UTSW	1	66,530,021 (GRCm38)	missense	possibly damaging	0.77
R5437:Unc80	UTSW	1	66,654,578 (GRCm38)	missense	possibly damaging	0.96
R5525:Unc80	UTSW	1	66,606,614 (GRCm38)	missense	possibly damaging	0.72
R5621:Unc80	UTSW	1	66,638,043 (GRCm38)	missense	possibly damaging	0.53
R5690:Unc80	UTSW	1	66,640,572 (GRCm38)	missense	probably benign	0.08
R5762:Unc80	UTSW	1	66,693,796 (GRCm38)	missense	possibly damaging	0.82
R5956:Unc80	UTSW	1	66,527,964 (GRCm38)	missense	probably damaging	0.97
R6005:Unc80	UTSW	1	66,627,257 (GRCm38)	missense	possibly damaging	0.53
R6025:Unc80	UTSW	1	66,695,568 (GRCm38)	missense	possibly damaging	0.90
R6033:Unc80	UTSW	1	66,473,260 (GRCm38)	missense	possibly damaging	0.92
R6033:Unc80	UTSW	1	66,473,260 (GRCm38)	missense	possibly damaging	0.92
R6117:Unc80	UTSW	1	66,675,067 (GRCm38)	missense	possibly damaging	0.72
R6156:Unc80	UTSW	1	66,612,250 (GRCm38)	missense	probably benign	0.01
R6157:Unc80	UTSW	1	66,654,029 (GRCm38)	nonsense	probably null
R6189:Unc80	UTSW	1	66,677,471 (GRCm38)	missense	probably benign	0.33
R6291:Unc80	UTSW	1	66,521,597 (GRCm38)	missense	possibly damaging	0.82
R6367:Unc80	UTSW	1	66,672,766 (GRCm38)	missense	probably benign	0.33
R6598:Unc80	UTSW	1	66,468,540 (GRCm38)	critical splice donor site	probably null
R6724:Unc80	UTSW	1	66,683,191 (GRCm38)	missense	possibly damaging	0.90
R6763:Unc80	UTSW	1	66,521,477 (GRCm38)	missense	probably benign	0.00
R6773:Unc80	UTSW	1	66,651,543 (GRCm38)	missense	probably benign	0.33
R6883:Unc80	UTSW	1	66,646,404 (GRCm38)	missense	probably benign	0.33
R6951:Unc80	UTSW	1	66,648,511 (GRCm38)	missense	possibly damaging	0.53
R6965:Unc80	UTSW	1	66,646,566 (GRCm38)	missense	probably benign	0.33
R6993:Unc80	UTSW	1	66,549,793 (GRCm38)	missense	possibly damaging	0.60
R7041:Unc80	UTSW	1	66,503,593 (GRCm38)	missense	probably benign	0.00
R7050:Unc80	UTSW	1	66,550,908 (GRCm38)	splice site	probably null
R7067:Unc80	UTSW	1	66,646,572 (GRCm38)	missense	possibly damaging	0.86
R7080:Unc80	UTSW	1	66,646,521 (GRCm38)	missense	possibly damaging	0.53
R7193:Unc80	UTSW	1	66,549,784 (GRCm38)	missense	possibly damaging	0.60
R7197:Unc80	UTSW	1	66,521,566 (GRCm38)	nonsense	probably null
R7278:Unc80	UTSW	1	66,552,209 (GRCm38)	missense	possibly damaging	0.82
R7290:Unc80	UTSW	1	66,601,197 (GRCm38)	missense	probably damaging	0.97
R7391:Unc80	UTSW	1	66,695,528 (GRCm38)	missense	probably benign	0.18
R7401:Unc80	UTSW	1	66,646,415 (GRCm38)	missense	possibly damaging	0.96
R7470:Unc80	UTSW	1	66,622,462 (GRCm38)	missense	probably benign	0.02
R7573:Unc80	UTSW	1	66,521,537 (GRCm38)	missense	probably damaging	1.00
R7637:Unc80	UTSW	1	66,672,684 (GRCm38)	missense	possibly damaging	0.86
R7678:Unc80	UTSW	1	66,649,722 (GRCm38)	missense	probably benign	0.33
R7697:Unc80	UTSW	1	66,637,945 (GRCm38)	missense	possibly damaging	0.93
R7746:Unc80	UTSW	1	66,677,385 (GRCm38)	missense	probably benign	0.33
R7768:Unc80	UTSW	1	66,510,595 (GRCm38)	missense	possibly damaging	0.56
R7796:Unc80	UTSW	1	66,503,714 (GRCm38)	missense	probably benign
R7855:Unc80	UTSW	1	66,483,349 (GRCm38)	missense	possibly damaging	0.78
R7878:Unc80	UTSW	1	66,601,141 (GRCm38)	missense	possibly damaging	0.88
R7879:Unc80	UTSW	1	66,510,707 (GRCm38)	missense	probably benign	0.00
R8024:Unc80	UTSW	1	66,606,644 (GRCm38)	missense	possibly damaging	0.86
R8026:Unc80	UTSW	1	66,483,304 (GRCm38)	missense	possibly damaging	0.92
R8115:Unc80	UTSW	1	66,648,913 (GRCm38)	missense	probably benign	0.00
R8135:Unc80	UTSW	1	66,509,287 (GRCm38)	missense	possibly damaging	0.49
R8170:Unc80	UTSW	1	66,651,533 (GRCm38)	missense	probably benign	0.33
R8239:Unc80	UTSW	1	66,654,019 (GRCm38)	missense	probably benign
R8249:Unc80	UTSW	1	66,619,491 (GRCm38)	missense	probably benign	0.01
R8275:Unc80	UTSW	1	66,640,614 (GRCm38)	nonsense	probably null
R8288:Unc80	UTSW	1	66,473,350 (GRCm38)	missense	probably benign	0.07
R8341:Unc80	UTSW	1	66,649,033 (GRCm38)	missense	possibly damaging	0.73
R8356:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8433:Unc80	UTSW	1	66,638,028 (GRCm38)	nonsense	probably null
R8456:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8464:Unc80	UTSW	1	66,473,264 (GRCm38)	missense	probably damaging	1.00
R8483:Unc80	UTSW	1	66,693,710 (GRCm38)	missense	possibly damaging	0.83
R8509:Unc80	UTSW	1	66,641,629 (GRCm38)	missense	possibly damaging	0.85
R8686:Unc80	UTSW	1	66,612,268 (GRCm38)	missense	possibly damaging	0.53
R8701:Unc80	UTSW	1	66,638,032 (GRCm38)	missense	possibly damaging	0.85
R8729:Unc80	UTSW	1	66,608,490 (GRCm38)	missense	probably benign	0.01
R8755:Unc80	UTSW	1	66,612,131 (GRCm38)	missense	possibly damaging	0.53
R8771:Unc80	UTSW	1	66,646,395 (GRCm38)	missense	possibly damaging	0.85
R8866:Unc80	UTSW	1	66,590,229 (GRCm38)	missense	probably benign	0.05
R8877:Unc80	UTSW	1	66,527,985 (GRCm38)	missense	possibly damaging	0.89
R8942:Unc80	UTSW	1	66,473,309 (GRCm38)	missense	possibly damaging	0.94
R8976:Unc80	UTSW	1	66,472,010 (GRCm38)	missense	possibly damaging	0.87
R9063:Unc80	UTSW	1	66,606,657 (GRCm38)	critical splice donor site	probably null
R9095:Unc80	UTSW	1	66,506,753 (GRCm38)	missense	probably damaging	1.00
R9125:Unc80	UTSW	1	66,679,581 (GRCm38)	missense	probably benign	0.18
R9130:Unc80	UTSW	1	66,638,085 (GRCm38)	missense	possibly damaging	0.85
R9165:Unc80	UTSW	1	66,549,841 (GRCm38)	missense	probably null	0.95
R9220:Unc80	UTSW	1	66,507,375 (GRCm38)	missense	probably damaging	1.00
R9262:Unc80	UTSW	1	66,555,252 (GRCm38)	intron	probably benign
R9334:Unc80	UTSW	1	66,649,760 (GRCm38)	missense	possibly damaging	0.73
R9374:Unc80	UTSW	1	66,590,301 (GRCm38)	missense	possibly damaging	0.95
R9387:Unc80	UTSW	1	66,549,938 (GRCm38)	critical splice donor site	probably null
R9415:Unc80	UTSW	1	66,510,905 (GRCm38)	missense
R9427:Unc80	UTSW	1	66,554,999 (GRCm38)	missense	probably damaging	1.00
R9436:Unc80	UTSW	1	66,693,805 (GRCm38)	critical splice donor site	probably null
R9454:Unc80	UTSW	1	66,695,590 (GRCm38)	missense	possibly damaging	0.53
R9522:Unc80	UTSW	1	66,638,062 (GRCm38)	missense	possibly damaging	0.73
R9539:Unc80	UTSW	1	66,570,004 (GRCm38)	critical splice donor site	probably null
R9552:Unc80	UTSW	1	66,678,123 (GRCm38)	missense	possibly damaging	0.85
R9667:Unc80	UTSW	1	66,612,128 (GRCm38)	missense	possibly damaging	0.86
R9720:Unc80	UTSW	1	66,644,326 (GRCm38)	missense	possibly damaging	0.53
R9749:Unc80	UTSW	1	66,505,020 (GRCm38)	missense	probably damaging	0.99
R9789:Unc80	UTSW	1	66,612,212 (GRCm38)	missense	possibly damaging	0.53
X0019:Unc80	UTSW	1	66,648,382 (GRCm38)	missense	probably benign	0.33
X0021:Unc80	UTSW	1	66,509,266 (GRCm38)	critical splice acceptor site	probably null
X0024:Unc80	UTSW	1	66,491,046 (GRCm38)	missense	probably benign	0.21
X0062:Unc80	UTSW	1	66,623,259 (GRCm38)	missense	probably benign	0.02
X0066:Unc80	UTSW	1	66,530,757 (GRCm38)	missense	possibly damaging	0.77
Y4335:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Y4336:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Y4338:Unc80	UTSW	1	66,521,581 (GRCm38)	missense	possibly damaging	0.46
Z1088:Unc80	UTSW	1	66,646,451 (GRCm38)	missense	possibly damaging	0.85
Z1176:Unc80	UTSW	1	66,694,409 (GRCm38)	missense	probably benign
Z1177:Unc80	UTSW	1	66,695,339 (GRCm38)	missense	probably benign	0.03
Z1177:Unc80	UTSW	1	66,646,398 (GRCm38)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- TGGGCCACTTTAGACAGTGTAAG -3'
(R):5'- AAGTAAATCAATTGCCCTGGAGG -3'

Sequencing Primer
(F):5'- GCCACTTTAGACAGTGTAAGAATAG -3'
(R):5'- CCCTGGAGGCAAAGAGACC -3'

Posted On

2014-09-17