Mutagenetix > Incidental Mutation

Incidental Mutation 'R0157:Cenpf'

22888

Institutional Source

Beutler Lab

Gene Symbol

Cenpf

Ensembl Gene

ENSMUSG00000026605

Gene Name

centromere protein F

Synonyms

6530404A22Rik, mitosin, Lek1

MMRRC Submission

038437-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.536) question?

Stock #

R0157 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

189372803-189420283 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 189384556 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 2575 (T2575S)

Ref Sequence

ENSEMBL: ENSMUSP00000129738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171929]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000165798

SMART Domains

Protein: ENSMUSP00000132430
Gene: ENSMUSG00000026605

Domain	Start	End	E-Value	Type
Pfam:CENP-F_C_Rb_bdg	23	50	1.6e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171929
AA Change: T2575S

PolyPhen 2 Score 0.073 (Sensitivity: 0.93; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000129738
Gene: ENSMUSG00000026605
AA Change: T2575S

Domain	Start	End	E-Value	Type
Pfam:CENP-F_N	1	300	2.8e-144	PFAM
low complexity region	349	364	N/A	INTRINSIC
low complexity region	378	396	N/A	INTRINSIC
low complexity region	544	557	N/A	INTRINSIC
low complexity region	589	609	N/A	INTRINSIC
internal_repeat_5	678	707	8.32e-5	PROSPERO
internal_repeat_3	743	798	2.21e-5	PROSPERO
internal_repeat_1	780	812	2.12e-7	PROSPERO
coiled coil region	829	881	N/A	INTRINSIC
coiled coil region	902	1169	N/A	INTRINSIC
coiled coil region	1206	1364	N/A	INTRINSIC
internal_repeat_2	1381	1413	3.02e-6	PROSPERO
internal_repeat_3	1412	1470	2.21e-5	PROSPERO
low complexity region	1526	1542	N/A	INTRINSIC
coiled coil region	1560	1650	N/A	INTRINSIC
internal_repeat_2	1655	1687	3.02e-6	PROSPERO
low complexity region	1744	1755	N/A	INTRINSIC
coiled coil region	1822	1852	N/A	INTRINSIC
Pfam:CENP-F_leu_zip	1893	2035	1.2e-14	PFAM
Pfam:CENP-F_leu_zip	2131	2270	1.5e-47	PFAM
Pfam:CENP-F_leu_zip	2313	2449	1e-46	PFAM
low complexity region	2544	2555	N/A	INTRINSIC
low complexity region	2642	2654	N/A	INTRINSIC
low complexity region	2755	2769	N/A	INTRINSIC
internal_repeat_4	2778	2801	4.28e-5	PROSPERO
low complexity region	2837	2847	N/A	INTRINSIC
Pfam:CENP-F_C_Rb_bdg	2850	2896	6.6e-29	PFAM
internal_repeat_1	2935	2964	2.12e-7	PROSPERO
internal_repeat_4	2946	2969	4.28e-5	PROSPERO

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 88.5%

Validation Efficiency

64% (47/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adap2	T	A	11: 80,056,527 (GRCm39)	I180N	probably damaging	Het
Alk	T	A	17: 72,256,840 (GRCm39)	N673I	probably benign	Het
Ankrd7	T	C	6: 18,866,539 (GRCm39)	S20P	probably damaging	Het
Arhgef26	T	G	3: 62,288,392 (GRCm39)	D487E	probably damaging	Het
Arhgef4	A	G	1: 34,845,475 (GRCm39)	D1500G	probably damaging	Het
Arhgef7	A	G	8: 11,835,812 (GRCm39)	I39V	probably damaging	Het
Asap2	T	A	12: 21,256,326 (GRCm39)	I208N	probably damaging	Het
Atad5	T	C	11: 79,980,643 (GRCm39)	V16A	possibly damaging	Het
Atp2b1	T	C	10: 98,835,809 (GRCm39)	I518T	probably damaging	Het
B130006D01Rik	T	C	11: 95,617,211 (GRCm39)		probably benign	Het
BC028528	A	G	3: 95,792,280 (GRCm39)		probably null	Het
Bpifb6	T	A	2: 153,745,886 (GRCm39)	L74Q	probably benign	Het
Bptf	T	C	11: 106,965,484 (GRCm39)	T1122A	possibly damaging	Het
Cacna2d4	T	A	6: 119,289,385 (GRCm39)	D806E	probably benign	Het
Cdhr3	T	C	12: 33,111,649 (GRCm39)	Q287R	possibly damaging	Het
Cdk12	A	G	11: 98,140,602 (GRCm39)		probably benign	Het
Chd7	T	A	4: 8,833,759 (GRCm39)	I1171N	probably damaging	Het
Chd9	T	C	8: 91,735,464 (GRCm39)		probably null	Het
Ckmt1	A	G	2: 121,193,522 (GRCm39)	T361A	possibly damaging	Het
Clec4d	G	T	6: 123,244,095 (GRCm39)	R68L	probably benign	Het
Csmd2	G	T	4: 128,415,704 (GRCm39)	V2678F	probably benign	Het
Cul7	T	A	17: 46,964,761 (GRCm39)	V131E	possibly damaging	Het
Dab2	T	C	15: 6,459,308 (GRCm39)	S407P	probably benign	Het
Dnah17	C	T	11: 118,017,997 (GRCm39)	G166D	probably benign	Het
F13b	G	A	1: 139,431,585 (GRCm39)	V52I	probably benign	Het
Gjd4	T	C	18: 9,280,549 (GRCm39)	I176M	probably benign	Het
Hoxc11	A	G	15: 102,863,436 (GRCm39)	Y159C	probably damaging	Het
Hydin	T	C	8: 111,026,642 (GRCm39)	I120T	possibly damaging	Het
Il20rb	A	G	9: 100,355,132 (GRCm39)	Y104H	probably damaging	Het
Krtap21-1	A	G	16: 89,200,430 (GRCm39)	C71R	unknown	Het
Lamc1	T	C	1: 153,138,353 (GRCm39)	D167G	probably benign	Het
Lin7c	C	A	2: 109,725,514 (GRCm39)	A73E	probably damaging	Het
Meiosin	T	C	7: 18,840,945 (GRCm39)	H63R	possibly damaging	Het
Mms22l	C	A	4: 24,588,224 (GRCm39)	A952E	probably damaging	Het
Myh3	A	G	11: 66,973,735 (GRCm39)	N136S	probably benign	Het
Ndufb10	T	C	17: 24,943,218 (GRCm39)	T31A	probably benign	Het
Nlrp2	T	C	7: 5,311,769 (GRCm39)	Y37C	possibly damaging	Het
Or2t44	T	C	11: 58,677,885 (GRCm39)	F275S	probably damaging	Het
Or2y14	T	C	11: 49,404,600 (GRCm39)	I45T	probably damaging	Het
Orc3	C	A	4: 34,607,130 (GRCm39)		probably null	Het
Pard3b	A	C	1: 62,250,792 (GRCm39)	M512L	probably damaging	Het
Pcdh10	A	G	3: 45,334,136 (GRCm39)	D150G	probably damaging	Het
Pcolce	A	T	5: 137,608,741 (GRCm39)		probably null	Het
Pdcl	A	C	2: 37,242,189 (GRCm39)	I187S	probably damaging	Het
Pkn1	T	C	8: 84,419,449 (GRCm39)	I51M	probably damaging	Het
Pla2g4e	T	A	2: 120,000,662 (GRCm39)	T692S	probably benign	Het
Plcb2	C	A	2: 118,549,022 (GRCm39)	V380F	probably damaging	Het
Pmpcb	A	T	5: 21,947,950 (GRCm39)	I218F	probably damaging	Het
Pms1	A	T	1: 53,234,196 (GRCm39)	Y773*	probably null	Het
Polr2e	C	T	10: 79,872,615 (GRCm39)	G184R	probably damaging	Het
Polr3a	T	C	14: 24,529,254 (GRCm39)	I369V	probably damaging	Het
Pramel21	C	T	4: 143,342,366 (GRCm39)	P158S	probably damaging	Het
Prpf4b	T	C	13: 35,068,014 (GRCm39)		probably benign	Het
Pzp	G	A	6: 128,500,939 (GRCm39)	Q140*	probably null	Het
Qrich2	T	A	11: 116,332,221 (GRCm39)	E2325V	probably damaging	Het
R3hdm2	T	G	10: 127,307,858 (GRCm39)	L373R	probably damaging	Het
Sema3d	A	T	5: 12,558,104 (GRCm39)	D212V	possibly damaging	Het
Sidt2	A	G	9: 45,850,565 (GRCm39)	I850T	probably damaging	Het
Slc22a29	C	T	19: 8,140,106 (GRCm39)	R433H	possibly damaging	Het
Slitrk6	A	T	14: 110,987,364 (GRCm39)	L781H	probably damaging	Het
Sox21	G	A	14: 118,473,354 (GRCm39)		probably benign	Het
Steap3	A	G	1: 120,155,379 (GRCm39)	*527R	probably null	Het
Svep1	T	C	4: 58,069,830 (GRCm39)	E2652G	possibly damaging	Het
Taar2	T	A	10: 23,817,389 (GRCm39)	F310I	probably damaging	Het
Tasor2	C	A	13: 3,625,550 (GRCm39)	V1467L	probably benign	Het
Tecta	A	G	9: 42,286,307 (GRCm39)	V783A	probably benign	Het
Vmn1r173	T	A	7: 23,401,822 (GRCm39)	I19N	probably damaging	Het
Vwa5b1	T	A	4: 138,332,190 (GRCm39)	M276L	probably benign	Het
Yeats2	A	C	16: 20,040,427 (GRCm39)	*142C	probably null	Het
Zfp26	G	T	9: 20,349,166 (GRCm39)	T466K	probably benign	Het
Zfp426	T	C	9: 20,382,432 (GRCm39)	N171S	probably benign	Het

Other mutations in Cenpf

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00778:Cenpf	APN	1	189,387,109 (GRCm39)	missense	probably benign	0.01
IGL01154:Cenpf	APN	1	189,412,530 (GRCm39)	missense	probably benign	0.19
IGL01434:Cenpf	APN	1	189,390,065 (GRCm39)	nonsense	probably null
IGL01461:Cenpf	APN	1	189,389,293 (GRCm39)	missense	probably damaging	1.00
IGL01615:Cenpf	APN	1	189,385,381 (GRCm39)	missense	possibly damaging	0.68
IGL01720:Cenpf	APN	1	189,383,412 (GRCm39)	missense	probably damaging	0.99
IGL01720:Cenpf	APN	1	189,414,583 (GRCm39)	missense	probably benign	0.05
IGL01803:Cenpf	APN	1	189,386,968 (GRCm39)	nonsense	probably null
IGL02152:Cenpf	APN	1	189,381,209 (GRCm39)	missense	probably benign
IGL02222:Cenpf	APN	1	189,386,641 (GRCm39)	missense	probably benign
IGL02338:Cenpf	APN	1	189,412,615 (GRCm39)	missense	probably damaging	1.00
IGL02580:Cenpf	APN	1	189,389,638 (GRCm39)	missense	probably benign	0.20
IGL02629:Cenpf	APN	1	189,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02650:Cenpf	APN	1	189,384,670 (GRCm39)	missense	possibly damaging	0.91
IGL02660:Cenpf	APN	1	189,386,979 (GRCm39)	missense	probably damaging	1.00
IGL02703:Cenpf	APN	1	189,391,955 (GRCm39)	missense	probably benign	0.14
IGL02809:Cenpf	APN	1	189,414,555 (GRCm39)	splice site	probably benign
IGL02851:Cenpf	APN	1	189,390,227 (GRCm39)	missense	probably damaging	1.00
IGL02903:Cenpf	APN	1	189,379,073 (GRCm39)	missense	probably damaging	0.99
IGL03126:Cenpf	APN	1	189,391,207 (GRCm39)	missense	probably damaging	1.00
IGL03235:Cenpf	APN	1	189,416,124 (GRCm39)	missense	probably damaging	1.00
IGL03336:Cenpf	APN	1	189,384,844 (GRCm39)	missense	probably damaging	0.99
IGL03402:Cenpf	APN	1	189,387,273 (GRCm39)	missense	probably damaging	1.00
IGL02799:Cenpf	UTSW	1	189,391,849 (GRCm39)	missense	probably damaging	1.00
R0011:Cenpf	UTSW	1	189,382,903 (GRCm39)	missense	probably benign	0.05
R0129:Cenpf	UTSW	1	189,391,847 (GRCm39)	missense	probably benign	0.26
R0270:Cenpf	UTSW	1	189,382,911 (GRCm39)	missense	probably benign	0.01
R0607:Cenpf	UTSW	1	189,414,660 (GRCm39)	splice site	probably null
R0621:Cenpf	UTSW	1	189,404,825 (GRCm39)	missense	probably benign
R0639:Cenpf	UTSW	1	189,390,259 (GRCm39)	missense	probably benign	0.01
R0653:Cenpf	UTSW	1	189,392,183 (GRCm39)	missense	probably damaging	1.00
R0718:Cenpf	UTSW	1	189,386,181 (GRCm39)	missense	probably damaging	1.00
R1157:Cenpf	UTSW	1	189,390,650 (GRCm39)	missense	probably benign	0.20
R1331:Cenpf	UTSW	1	189,374,998 (GRCm39)	missense	probably damaging	0.99
R1463:Cenpf	UTSW	1	189,386,936 (GRCm39)	missense	probably damaging	0.97
R1514:Cenpf	UTSW	1	189,411,338 (GRCm39)	missense	possibly damaging	0.67
R1529:Cenpf	UTSW	1	189,392,235 (GRCm39)	missense	probably benign	0.00
R1574:Cenpf	UTSW	1	189,384,910 (GRCm39)	missense	probably damaging	1.00
R1574:Cenpf	UTSW	1	189,384,910 (GRCm39)	missense	probably damaging	1.00
R1662:Cenpf	UTSW	1	189,389,968 (GRCm39)	missense	probably damaging	0.99
R1671:Cenpf	UTSW	1	189,411,341 (GRCm39)	splice site	probably null
R1725:Cenpf	UTSW	1	189,412,676 (GRCm39)	missense	probably damaging	0.99
R1743:Cenpf	UTSW	1	189,386,460 (GRCm39)	missense	probably benign	0.19
R1874:Cenpf	UTSW	1	189,416,013 (GRCm39)	missense	probably damaging	1.00
R1884:Cenpf	UTSW	1	189,379,046 (GRCm39)	missense	probably benign
R1980:Cenpf	UTSW	1	189,386,112 (GRCm39)	missense	probably benign	0.04
R2074:Cenpf	UTSW	1	189,389,098 (GRCm39)	missense	probably damaging	1.00
R2096:Cenpf	UTSW	1	189,385,656 (GRCm39)	missense	possibly damaging	0.95
R2109:Cenpf	UTSW	1	189,411,264 (GRCm39)	missense	probably damaging	0.99
R2113:Cenpf	UTSW	1	189,411,299 (GRCm39)	missense	probably damaging	0.96
R2134:Cenpf	UTSW	1	189,390,839 (GRCm39)	missense	probably benign	0.03
R2209:Cenpf	UTSW	1	189,384,795 (GRCm39)	missense	probably benign	0.04
R2875:Cenpf	UTSW	1	189,390,841 (GRCm39)	missense	probably benign	0.11
R2876:Cenpf	UTSW	1	189,390,841 (GRCm39)	missense	probably benign	0.11
R3433:Cenpf	UTSW	1	189,392,146 (GRCm39)	missense	probably damaging	0.99
R3709:Cenpf	UTSW	1	189,381,009 (GRCm39)	missense	possibly damaging	0.72
R3786:Cenpf	UTSW	1	189,390,534 (GRCm39)	missense	probably damaging	1.00
R4014:Cenpf	UTSW	1	189,385,356 (GRCm39)	missense	probably benign	0.01
R4108:Cenpf	UTSW	1	189,416,065 (GRCm39)	missense	probably damaging	1.00
R4119:Cenpf	UTSW	1	189,385,242 (GRCm39)	missense	probably benign	0.01
R4177:Cenpf	UTSW	1	189,400,816 (GRCm39)	missense	possibly damaging	0.95
R4422:Cenpf	UTSW	1	189,390,547 (GRCm39)	missense	probably damaging	1.00
R4546:Cenpf	UTSW	1	189,386,847 (GRCm39)	missense	probably damaging	1.00
R4592:Cenpf	UTSW	1	189,411,230 (GRCm39)	missense	probably damaging	1.00
R4643:Cenpf	UTSW	1	189,391,786 (GRCm39)	missense	probably benign	0.00
R4650:Cenpf	UTSW	1	189,392,235 (GRCm39)	missense	probably benign	0.00
R4801:Cenpf	UTSW	1	189,383,417 (GRCm39)	missense	probably damaging	1.00
R4802:Cenpf	UTSW	1	189,383,417 (GRCm39)	missense	probably damaging	1.00
R4817:Cenpf	UTSW	1	189,414,566 (GRCm39)	missense	possibly damaging	0.93
R4871:Cenpf	UTSW	1	189,390,728 (GRCm39)	missense	probably damaging	1.00
R5037:Cenpf	UTSW	1	189,416,043 (GRCm39)	missense	probably damaging	1.00
R5106:Cenpf	UTSW	1	189,416,005 (GRCm39)	missense	probably benign	0.00
R5208:Cenpf	UTSW	1	189,403,243 (GRCm39)	critical splice donor site	probably null
R5213:Cenpf	UTSW	1	189,387,177 (GRCm39)	missense	probably benign	0.04
R5237:Cenpf	UTSW	1	189,391,730 (GRCm39)	missense	probably benign	0.28
R5255:Cenpf	UTSW	1	189,404,824 (GRCm39)	missense	possibly damaging	0.49
R5378:Cenpf	UTSW	1	189,385,663 (GRCm39)	missense	possibly damaging	0.95
R5468:Cenpf	UTSW	1	189,384,568 (GRCm39)	missense	probably damaging	1.00
R5510:Cenpf	UTSW	1	189,415,100 (GRCm39)	missense	probably benign	0.14
R5616:Cenpf	UTSW	1	189,389,483 (GRCm39)	missense	probably damaging	1.00
R5652:Cenpf	UTSW	1	189,389,279 (GRCm39)	missense	probably damaging	0.99
R5735:Cenpf	UTSW	1	189,386,560 (GRCm39)	missense	probably benign	0.10
R5841:Cenpf	UTSW	1	189,389,641 (GRCm39)	missense	possibly damaging	0.90
R5943:Cenpf	UTSW	1	189,392,166 (GRCm39)	missense	possibly damaging	0.75
R6082:Cenpf	UTSW	1	189,390,301 (GRCm39)	missense	probably benign	0.11
R6108:Cenpf	UTSW	1	189,394,210 (GRCm39)	missense	probably benign	0.03
R6269:Cenpf	UTSW	1	189,392,117 (GRCm39)	missense	probably benign	0.37
R6284:Cenpf	UTSW	1	189,384,939 (GRCm39)	missense	probably damaging	1.00
R6425:Cenpf	UTSW	1	189,392,095 (GRCm39)	missense	probably benign	0.09
R6587:Cenpf	UTSW	1	189,390,571 (GRCm39)	missense	probably damaging	1.00
R6747:Cenpf	UTSW	1	189,385,051 (GRCm39)	missense	probably benign	0.15
R6811:Cenpf	UTSW	1	189,386,739 (GRCm39)	missense	probably benign	0.06
R6834:Cenpf	UTSW	1	189,391,643 (GRCm39)	missense	probably damaging	1.00
R6951:Cenpf	UTSW	1	189,385,989 (GRCm39)	missense	probably damaging	1.00
R7095:Cenpf	UTSW	1	189,391,373 (GRCm39)	missense	probably benign	0.01
R7128:Cenpf	UTSW	1	189,417,188 (GRCm39)	missense	probably damaging	1.00
R7185:Cenpf	UTSW	1	189,385,686 (GRCm39)	missense	probably damaging	1.00
R7292:Cenpf	UTSW	1	189,382,891 (GRCm39)	missense	probably damaging	1.00
R7353:Cenpf	UTSW	1	189,386,335 (GRCm39)	nonsense	probably null
R7402:Cenpf	UTSW	1	189,391,575 (GRCm39)	nonsense	probably null
R7460:Cenpf	UTSW	1	189,386,247 (GRCm39)	missense	probably damaging	0.97
R7484:Cenpf	UTSW	1	189,389,018 (GRCm39)	missense	probably damaging	1.00
R7574:Cenpf	UTSW	1	189,390,864 (GRCm39)	missense	probably damaging	1.00
R7691:Cenpf	UTSW	1	189,390,404 (GRCm39)	nonsense	probably null
R7698:Cenpf	UTSW	1	189,394,269 (GRCm39)	missense	probably benign	0.01
R7901:Cenpf	UTSW	1	189,389,445 (GRCm39)	missense	probably damaging	1.00
R7941:Cenpf	UTSW	1	189,389,483 (GRCm39)	missense	probably damaging	1.00
R8007:Cenpf	UTSW	1	189,379,144 (GRCm39)	missense
R8194:Cenpf	UTSW	1	189,414,600 (GRCm39)	missense	probably benign	0.06
R8420:Cenpf	UTSW	1	189,404,782 (GRCm39)	missense	probably damaging	1.00
R8429:Cenpf	UTSW	1	189,389,504 (GRCm39)	missense	possibly damaging	0.72
R8477:Cenpf	UTSW	1	189,385,385 (GRCm39)	missense	probably benign
R8492:Cenpf	UTSW	1	189,390,926 (GRCm39)	missense	probably damaging	0.99
R8510:Cenpf	UTSW	1	189,382,903 (GRCm39)	missense	probably benign	0.01
R8686:Cenpf	UTSW	1	189,391,801 (GRCm39)	missense	probably benign	0.00
R8696:Cenpf	UTSW	1	189,390,194 (GRCm39)	missense	probably benign	0.20
R8855:Cenpf	UTSW	1	189,385,430 (GRCm39)	missense	probably benign	0.11
R8901:Cenpf	UTSW	1	189,394,248 (GRCm39)	missense	probably benign	0.30
R8958:Cenpf	UTSW	1	189,385,350 (GRCm39)	missense	possibly damaging	0.81
R9109:Cenpf	UTSW	1	189,391,571 (GRCm39)	missense	probably benign	0.06
R9135:Cenpf	UTSW	1	189,404,746 (GRCm39)	missense	probably damaging	1.00
R9136:Cenpf	UTSW	1	189,403,352 (GRCm39)	missense	probably benign	0.02
R9198:Cenpf	UTSW	1	189,388,987 (GRCm39)	missense	probably damaging	1.00
R9240:Cenpf	UTSW	1	189,389,167 (GRCm39)	missense	probably benign	0.01
R9303:Cenpf	UTSW	1	189,392,271 (GRCm39)	critical splice acceptor site	probably null
R9305:Cenpf	UTSW	1	189,392,271 (GRCm39)	critical splice acceptor site	probably null
R9354:Cenpf	UTSW	1	189,379,114 (GRCm39)	missense
R9502:Cenpf	UTSW	1	189,388,978 (GRCm39)	missense	probably damaging	1.00
R9619:Cenpf	UTSW	1	189,385,965 (GRCm39)	missense	probably benign	0.01
RF006:Cenpf	UTSW	1	189,389,583 (GRCm39)	missense	probably damaging	1.00
X0025:Cenpf	UTSW	1	189,386,071 (GRCm39)	missense	possibly damaging	0.79
X0066:Cenpf	UTSW	1	189,390,126 (GRCm39)	missense	probably benign	0.23
Z1088:Cenpf	UTSW	1	189,385,128 (GRCm39)	missense	probably damaging	1.00
Z1176:Cenpf	UTSW	1	189,391,669 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCTGCCTGAGCTGACTCAATG -3'
(R):5'- GCCCTCACTCAAGAAGTAGAAGCTG -3'

Sequencing Primer
(F):5'- GACTCAATGTCCCTGTAGTCAAG -3'
(R):5'- CTCAAGAAGTAGAAGCTGGCAAAC -3'

Posted On

2013-04-16