Mutagenetix > Incidental Mutation

Incidental Mutation 'R2064:Slc4a7'

229006

Institutional Source

Beutler Lab

Gene Symbol

Slc4a7

Ensembl Gene

ENSMUSG00000021733

Gene Name

solute carrier family 4, sodium bicarbonate cotransporter, member 7

Synonyms

NBC3, NBCn1, E430014N10Rik

MMRRC Submission

040069-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.925) question?

Stock #

R2064 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

7669819-7766808 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 14733773 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 67 (R67H)

Ref Sequence

ENSEMBL: ENSMUSP00000153470 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057015] [ENSMUST00000223607] [ENSMUST00000223695] [ENSMUST00000223740] [ENSMUST00000223761] [ENSMUST00000223981] [ENSMUST00000224049] [ENSMUST00000224333] [ENSMUST00000225175] [ENSMUST00000224752] [ENSMUST00000224672] [ENSMUST00000225232] [ENSMUST00000225238] [ENSMUST00000224222] [ENSMUST00000225979] [ENSMUST00000226079] [ENSMUST00000225630]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000057015
AA Change: R61H

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000058313
Gene: ENSMUSG00000021733
AA Change: R61H

Domain	Start	End	E-Value	Type
low complexity region	57	89	N/A	INTRINSIC
Pfam:Band_3_cyto	146	413	1.4e-110	PFAM
Pfam:HCO3_cotransp	456	969	1.6e-242	PFAM
transmembrane domain	977	999	N/A	INTRINSIC
coiled coil region	1021	1050	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000223607
AA Change: R61H

PolyPhen 2 Score 0.093 (Sensitivity: 0.93; Specificity: 0.85)

Predicted Effect

probably benign
Transcript: ENSMUST00000223695
AA Change: R67H

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

Predicted Effect

probably benign
Transcript: ENSMUST00000223740
AA Change: R67H

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000223761
AA Change: R61H

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

Predicted Effect

probably damaging
Transcript: ENSMUST00000223981
AA Change: R61H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

probably benign
Transcript: ENSMUST00000224049
AA Change: R67H

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

Predicted Effect

probably damaging
Transcript: ENSMUST00000224333
AA Change: R67H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

probably benign
Transcript: ENSMUST00000225175
AA Change: R61H

PolyPhen 2 Score 0.099 (Sensitivity: 0.93; Specificity: 0.85)

Predicted Effect

probably benign
Transcript: ENSMUST00000224752
AA Change: R66H

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000224672
AA Change: R67H

PolyPhen 2 Score 0.766 (Sensitivity: 0.85; Specificity: 0.92)

Predicted Effect

probably benign
Transcript: ENSMUST00000225232
AA Change: R61H

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000225238
AA Change: R61H

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000224222
AA Change: R61H

PolyPhen 2 Score 0.093 (Sensitivity: 0.93; Specificity: 0.85)

Predicted Effect

probably benign
Transcript: ENSMUST00000225979
AA Change: R61H

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000226079
AA Change: R61H

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

unknown
Transcript: ENSMUST00000224952
AA Change: R80H

Predicted Effect

probably benign
Transcript: ENSMUST00000225630
AA Change: R61H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224197

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225496

Meta Mutation Damage Score

0.0747

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.3%

Validation Efficiency

99% (114/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This locus encodes a sodium bicarbonate cotransporter. The encoded transmembrane protein appears to transport sodium and bicarbonate ions in a 1:1 ratio, and is thus considered an electroneutral cotransporter. The encoded protein likely plays a critical role in regulation of intracellular pH involved in visual and auditory sensory transmission. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]
PHENOTYPE: Mice homozygous for a disruption at this locus display defects of the auditory and visual systems similar to those observed in patients with Ushers syndrome. Mice homozygous for a gene trap allele exhibit disruption in sodium/bicarbonate function that impacts vasodilation and hypertension. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933436I01Rik	T	A	X: 66,964,308 (GRCm39)	I184L	probably benign	Het
9530002B09Rik	T	A	4: 122,583,115 (GRCm39)		probably benign	Het
Actr1b	A	G	1: 36,741,168 (GRCm39)	F138L	possibly damaging	Het
Adamts14	G	T	10: 61,041,301 (GRCm39)	P803Q	probably benign	Het
Afap1l1	A	T	18: 61,872,193 (GRCm39)		probably null	Het
Akt3	A	G	1: 176,930,551 (GRCm39)	S136P	possibly damaging	Het
Aldh16a1	C	T	7: 44,796,585 (GRCm39)		probably null	Het
Alg12	A	G	15: 88,696,318 (GRCm39)	W238R	probably damaging	Het
Amy2a1	T	C	3: 113,324,217 (GRCm39)	I108V	probably benign	Het
Arhgap23	T	A	11: 97,383,888 (GRCm39)	C1144S	probably benign	Het
Arhgef40	G	A	14: 52,233,640 (GRCm39)	V829M	probably damaging	Het
Atg7	C	A	6: 114,680,324 (GRCm39)	N344K	probably damaging	Het
Aunip	T	A	4: 134,250,618 (GRCm39)	S188T	probably benign	Het
Bmpr1b	A	T	3: 141,576,568 (GRCm39)	H88Q	probably benign	Het
Brpf3	G	A	17: 29,040,338 (GRCm39)	G920S	probably benign	Het
Cdc123	C	T	2: 5,800,354 (GRCm39)		probably benign	Het
Cdhr1	T	C	14: 36,817,062 (GRCm39)	R100G	probably benign	Het
Cdkn2d	A	G	9: 21,202,175 (GRCm39)	V24A	probably damaging	Het
Cirbp	T	C	10: 80,006,166 (GRCm39)		probably benign	Het
Clptm1l	C	T	13: 73,755,842 (GRCm39)	Q153*	probably null	Het
Col12a1	A	G	9: 79,569,736 (GRCm39)		probably benign	Het
Cplane1	T	A	15: 8,215,649 (GRCm39)	S402T	probably damaging	Het
Cpox	T	A	16: 58,494,772 (GRCm39)	C270S	probably benign	Het
Cspg4	A	G	9: 56,803,940 (GRCm39)	D1677G	probably damaging	Het
Cyp4a10	T	A	4: 115,381,917 (GRCm39)		probably benign	Het
Dctn4	T	C	18: 60,671,349 (GRCm39)	F74L	possibly damaging	Het
Dennd5a	G	A	7: 109,497,900 (GRCm39)		probably benign	Het
Dnaaf3	T	C	7: 4,526,798 (GRCm39)	I426M	possibly damaging	Het
Dnah9	C	T	11: 66,036,261 (GRCm39)	S185N	probably benign	Het
Dqx1	T	G	6: 83,035,524 (GRCm39)		probably benign	Het
Dtx2	C	T	5: 136,059,431 (GRCm39)	S493F	probably damaging	Het
Ehd1	T	C	19: 6,348,108 (GRCm39)	L362P	probably benign	Het
Endov	T	C	11: 119,390,408 (GRCm39)	F12S	probably damaging	Het
Ep400	A	C	5: 110,883,270 (GRCm39)		probably benign	Het
Epha1	G	T	6: 42,342,987 (GRCm39)	H187Q	probably benign	Het
Exoc2	T	C	13: 31,119,544 (GRCm39)	D119G	probably benign	Het
Fbn2	C	T	18: 58,181,921 (GRCm39)	E1827K	probably damaging	Het
Fbxo41	C	T	6: 85,455,453 (GRCm39)	W577*	probably null	Het
Fgd6	G	A	10: 93,880,903 (GRCm39)	A586T	probably damaging	Het
Gm12695	T	C	4: 96,657,963 (GRCm39)	T69A	probably benign	Het
Gm12888	A	T	4: 121,182,069 (GRCm39)	W8R	unknown	Het
Gm2959	A	G	14: 42,235,658 (GRCm39)		noncoding transcript	Het
Gm6214	A	G	3: 140,544,978 (GRCm39)		noncoding transcript	Het
Gm9912	T	C	3: 148,890,795 (GRCm39)	T113A	unknown	Het
Gmeb2	A	G	2: 180,895,763 (GRCm39)	L469P	probably benign	Het
Gosr1	T	C	11: 76,628,224 (GRCm39)	I177V	probably benign	Het
Gria1	T	C	11: 57,208,534 (GRCm39)	F810L	probably damaging	Het
Gtf2ird2	C	T	5: 134,245,340 (GRCm39)	Q533*	probably null	Het
Hadhb	T	A	5: 30,378,796 (GRCm39)		probably null	Het
Hat1	A	T	2: 71,240,504 (GRCm39)	Y66F	possibly damaging	Het
Hk1	T	C	10: 62,122,315 (GRCm39)	Y488C	probably benign	Het
Htt	C	T	5: 34,983,326 (GRCm39)	T975I	probably benign	Het
Ifnb1	T	A	4: 88,440,996 (GRCm39)	I6F	possibly damaging	Het
Il19	T	C	1: 130,866,854 (GRCm39)	H42R	probably benign	Het
Il2rg	A	G	X: 100,311,416 (GRCm39)	L57P	possibly damaging	Het
Impg2	G	A	16: 56,063,993 (GRCm39)		probably null	Het
Invs	T	A	4: 48,396,287 (GRCm39)	L320Q	probably damaging	Het
Itga9	T	C	9: 118,636,361 (GRCm39)	F683S	probably damaging	Het
Itih2	A	G	2: 10,135,385 (GRCm39)	S2P	possibly damaging	Het
Itpr3	G	A	17: 27,317,050 (GRCm39)	M768I	probably benign	Het
Kcnab1	A	G	3: 65,272,060 (GRCm39)	E315G	probably benign	Het
Kcnmb2	A	C	3: 32,252,437 (GRCm39)	I213L	probably damaging	Het
Khdc1a	T	A	1: 21,421,196 (GRCm39)	M127K	probably benign	Het
Klhl28	T	C	12: 64,990,246 (GRCm39)	N565S	probably benign	Het
Lcp1	A	G	14: 75,435,515 (GRCm39)		probably null	Het
Mcm4	T	C	16: 15,452,333 (GRCm39)	T267A	possibly damaging	Het
Mlxipl	A	T	5: 135,161,631 (GRCm39)	T517S	possibly damaging	Het
Mpp3	T	A	11: 101,891,516 (GRCm39)	I541L	probably benign	Het
Mpp4	G	A	1: 59,182,941 (GRCm39)	P322L	possibly damaging	Het
Myh15	G	A	16: 48,975,984 (GRCm39)	A1351T	possibly damaging	Het
Nacc1	A	T	8: 85,399,747 (GRCm39)	M490K	probably benign	Het
Nbeal1	C	A	1: 60,309,515 (GRCm39)	Q496K	possibly damaging	Het
Oas1g	T	C	5: 121,023,946 (GRCm39)	E121G	probably damaging	Het
Or14j1	T	A	17: 38,145,893 (GRCm39)	M1K	probably null	Het
Pard3	T	C	8: 128,337,092 (GRCm39)	L1236P	probably damaging	Het
Phc2	T	C	4: 128,640,929 (GRCm39)	F672S	probably damaging	Het
Phf21a	C	A	2: 92,157,422 (GRCm39)	N183K	possibly damaging	Het
Plxnb3	T	A	X: 72,815,357 (GRCm39)	Y1845*	probably null	Het
Prl3c1	G	A	13: 27,380,720 (GRCm39)		probably null	Het
Prl7a2	T	A	13: 27,844,870 (GRCm39)	Y172F	probably damaging	Het
Psg16	T	G	7: 16,827,673 (GRCm39)	S210A	possibly damaging	Het
Psg25	T	A	7: 18,255,178 (GRCm39)	K446M	probably damaging	Het
Ptprz1	A	G	6: 23,050,388 (GRCm39)		probably benign	Het
Rgs7	A	T	1: 174,949,508 (GRCm39)	F160L	probably damaging	Het
Rpl22l1	A	T	3: 28,860,957 (GRCm39)	E57D	possibly damaging	Het
Rpl35	A	G	2: 38,894,753 (GRCm39)	L44P	possibly damaging	Het
Rtn4r	T	C	16: 17,969,121 (GRCm39)	L183P	probably damaging	Het
Skint1	T	A	4: 111,882,730 (GRCm39)	V258D	probably benign	Het
Smg1	C	T	7: 117,756,090 (GRCm39)		probably benign	Het
Smurf2	T	C	11: 106,762,374 (GRCm39)	T39A	probably damaging	Het
Sspo	A	T	6: 48,450,596 (GRCm39)	D2595V	probably damaging	Het
Tatdn2	A	G	6: 113,681,103 (GRCm39)	K379E	probably benign	Het
Tbc1d14	G	A	5: 36,680,274 (GRCm39)	R68*	probably null	Het
Tgm1	A	G	14: 55,946,928 (GRCm39)	I360T	probably damaging	Het
Thrap3	T	C	4: 126,069,189 (GRCm39)	Y654C	possibly damaging	Het
Traf1	A	T	2: 34,838,202 (GRCm39)	I212N	probably benign	Het
Ttc17	C	A	2: 94,196,892 (GRCm39)	W485L	probably damaging	Het
Ttn	C	A	2: 76,768,675 (GRCm39)	V2920F	probably damaging	Het
Tyr	T	C	7: 87,142,051 (GRCm39)	I93V	probably benign	Het
Ubap2	C	T	4: 41,199,872 (GRCm39)	A752T	probably benign	Het
Vmn1r212	A	G	13: 23,068,285 (GRCm39)	V16A	probably benign	Het
Vmn1r55	A	G	7: 5,150,048 (GRCm39)	V125A	possibly damaging	Het
Vmn2r120	T	A	17: 57,831,553 (GRCm39)	H412L	possibly damaging	Het
Xirp1	T	C	9: 119,845,962 (GRCm39)	I974V	probably benign	Het
Zap70	A	G	1: 36,818,215 (GRCm39)	T301A	probably benign	Het
Zfhx4	A	G	3: 5,463,987 (GRCm39)	K1382E	probably damaging	Het
Zfp280b	T	A	10: 75,875,017 (GRCm39)	C299S	probably damaging	Het
Zfp352	C	T	4: 90,113,357 (GRCm39)	S499L	probably benign	Het
Zfp423	G	A	8: 88,507,986 (GRCm39)	A661V	probably benign	Het
Zfp57	T	A	17: 37,320,568 (GRCm39)	F141I	possibly damaging	Het
Zfp946	A	T	17: 22,674,446 (GRCm39)	H400L	probably damaging	Het
Zgrf1	T	C	3: 127,406,999 (GRCm39)	C1589R	probably damaging	Het

Other mutations in Slc4a7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00974:Slc4a7	APN	14	14,760,292 (GRCm38)	missense	probably benign	0.18
IGL01468:Slc4a7	APN	14	14,737,480 (GRCm38)	missense	probably damaging	1.00
IGL01863:Slc4a7	APN	14	14,762,430 (GRCm38)	missense	probably damaging	0.97
IGL03122:Slc4a7	APN	14	14,782,040 (GRCm38)	splice site	probably benign
R0020:Slc4a7	UTSW	14	14,796,108 (GRCm38)	missense	probably benign
R0403:Slc4a7	UTSW	14	14,766,808 (GRCm38)	missense	probably benign	0.02
R0410:Slc4a7	UTSW	14	14,738,299 (GRCm38)	missense	probably damaging	1.00
R0624:Slc4a7	UTSW	14	14,794,059 (GRCm38)	critical splice donor site	probably null
R0631:Slc4a7	UTSW	14	14,757,382 (GRCm38)	missense	probably damaging	1.00
R1128:Slc4a7	UTSW	14	14,733,832 (GRCm38)	missense	probably damaging	1.00
R1556:Slc4a7	UTSW	14	14,778,872 (GRCm38)	missense	probably benign	0.01
R1672:Slc4a7	UTSW	14	14,760,247 (GRCm38)	missense	possibly damaging	0.91
R1711:Slc4a7	UTSW	14	14,765,709 (GRCm38)	missense	probably benign	0.45
R1870:Slc4a7	UTSW	14	14,737,509 (GRCm38)	critical splice donor site	probably null
R1939:Slc4a7	UTSW	14	14,748,581 (GRCm38)	missense	probably damaging	1.00
R2012:Slc4a7	UTSW	14	14,733,727 (GRCm38)	nonsense	probably null
R2042:Slc4a7	UTSW	14	14,737,386 (GRCm38)	missense	probably damaging	1.00
R2404:Slc4a7	UTSW	14	14,733,733 (GRCm38)	missense	probably damaging	1.00
R2880:Slc4a7	UTSW	14	14,773,277 (GRCm38)	missense	probably damaging	1.00
R3729:Slc4a7	UTSW	14	14,729,276 (GRCm38)	missense	probably damaging	1.00
R4368:Slc4a7	UTSW	14	14,733,775 (GRCm38)	missense	probably damaging	1.00
R4395:Slc4a7	UTSW	14	14,765,665 (GRCm38)	missense	probably damaging	1.00
R4432:Slc4a7	UTSW	14	14,757,323 (GRCm38)	missense	probably damaging	1.00
R4592:Slc4a7	UTSW	14	14,778,850 (GRCm38)	missense	probably damaging	1.00
R4705:Slc4a7	UTSW	14	14,733,856 (GRCm38)	missense	probably damaging	1.00
R4743:Slc4a7	UTSW	14	14,796,073 (GRCm38)	splice site	probably null
R4765:Slc4a7	UTSW	14	14,762,414 (GRCm38)	missense	probably damaging	1.00
R4831:Slc4a7	UTSW	14	14,772,699 (GRCm38)	critical splice donor site	probably null
R4845:Slc4a7	UTSW	14	14,733,803 (GRCm38)	missense	probably damaging	1.00
R4880:Slc4a7	UTSW	14	14,757,342 (GRCm38)	missense	probably damaging	1.00
R4948:Slc4a7	UTSW	14	14,771,283 (GRCm38)	missense	possibly damaging	0.68
R5348:Slc4a7	UTSW	14	14,786,310 (GRCm38)	missense	probably benign	0.02
R5385:Slc4a7	UTSW	14	14,773,345 (GRCm38)	missense	possibly damaging	0.94
R5418:Slc4a7	UTSW	14	14,760,280 (GRCm38)	missense	probably benign	0.25
R5480:Slc4a7	UTSW	14	14,782,138 (GRCm38)	missense	probably damaging	1.00
R5842:Slc4a7	UTSW	14	14,778,866 (GRCm38)	missense	probably damaging	1.00
R5919:Slc4a7	UTSW	14	14,791,092 (GRCm38)	missense	probably benign
R6063:Slc4a7	UTSW	14	14,793,964 (GRCm38)	missense	possibly damaging	0.60
R6065:Slc4a7	UTSW	14	14,739,836 (GRCm38)	missense	probably benign	0.29
R6549:Slc4a7	UTSW	14	14,748,564 (GRCm38)	missense	probably damaging	1.00
R6845:Slc4a7	UTSW	14	14,775,000 (GRCm38)	missense	probably damaging	1.00
R6870:Slc4a7	UTSW	14	14,733,846 (GRCm38)	missense	probably damaging	1.00
R6881:Slc4a7	UTSW	14	14,737,452 (GRCm38)	missense	probably benign	0.43
R6962:Slc4a7	UTSW	14	14,746,021 (GRCm38)	missense	probably damaging	0.99
R7099:Slc4a7	UTSW	14	14,733,750 (GRCm38)	missense	probably damaging	1.00
R7180:Slc4a7	UTSW	14	14,765,580 (GRCm38)	missense	probably damaging	1.00
R7346:Slc4a7	UTSW	14	14,775,000 (GRCm38)	missense	probably damaging	1.00
R7378:Slc4a7	UTSW	14	14,757,421 (GRCm38)	missense	probably damaging	1.00
R7646:Slc4a7	UTSW	14	14,773,348 (GRCm38)	missense	probably benign	0.01
R7647:Slc4a7	UTSW	14	14,773,348 (GRCm38)	missense	probably benign	0.01
R7648:Slc4a7	UTSW	14	14,773,348 (GRCm38)	missense	probably benign	0.01
R7650:Slc4a7	UTSW	14	14,773,348 (GRCm38)	missense	probably benign	0.01
R7857:Slc4a7	UTSW	14	14,772,624 (GRCm38)	missense	probably benign	0.00
R7892:Slc4a7	UTSW	14	14,773,348 (GRCm38)	missense	probably benign	0.01
R8124:Slc4a7	UTSW	14	14,729,211 (GRCm38)	missense	possibly damaging	0.92
R8225:Slc4a7	UTSW	14	14,738,224 (GRCm38)	nonsense	probably null
R8354:Slc4a7	UTSW	14	14,786,313 (GRCm38)	missense	probably damaging	1.00
R8998:Slc4a7	UTSW	14	14,775,346 (GRCm38)	missense	probably damaging	1.00
R9016:Slc4a7	UTSW	14	14,773,241 (GRCm38)	missense	probably damaging	0.99
R9043:Slc4a7	UTSW	14	14,775,048 (GRCm38)	missense	probably damaging	1.00
R9139:Slc4a7	UTSW	14	14,796,115 (GRCm38)	missense	probably damaging	0.98
R9342:Slc4a7	UTSW	14	14,772,541 (GRCm38)	nonsense	probably null
R9383:Slc4a7	UTSW	14	14,766,803 (GRCm38)	nonsense	probably null
R9568:Slc4a7	UTSW	14	14,796,073 (GRCm38)	splice site	probably null
R9798:Slc4a7	UTSW	14	14,782,056 (GRCm38)	missense	probably damaging	1.00
X0067:Slc4a7	UTSW	14	14,771,276 (GRCm38)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- CATCGTGACTTTGAGCTCACATG -3'
(R):5'- ACACTCATGCACCAGATTCTG -3'

Sequencing Primer
(F):5'- CGTGACTTTGAGCTCACATGTAATTC -3'
(R):5'- TAGACCCCGCTGTAGAAATGCTTG -3'

Posted On

2014-09-17