Mutagenetix > Incidental Mutation

Incidental Mutation 'R2077:Vmn1r158'

229213

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r158

Ensembl Gene

ENSMUSG00000094700

Gene Name

vomeronasal 1 receptor 158

Synonyms

Gm16455

MMRRC Submission

040082-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.422) question?

Stock #

R2077 (G1)

Quality Score

202

Status

Validated

Chromosome

Chromosomal Location

22789859-22790782 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 22790390 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 131 (R131S)

Ref Sequence

ENSEMBL: ENSMUSP00000133990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000174643]

AlphaFold

G3UY92

Predicted Effect

probably benign
Transcript: ENSMUST00000174643
AA Change: R131S

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000133990
Gene: ENSMUSG00000094700
AA Change: R131S

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	298	2.9e-15	PFAM
Pfam:7tm_1	39	290	5.5e-9	PFAM
Pfam:V1R	41	298	3.2e-17	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

98% (48/49)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	T	A	4: 144,457,034 (GRCm38)		probably benign	Het
Abhd16b	A	C	2: 181,493,416 (GRCm38)	D37A	probably benign	Het
Acp7	T	C	7: 28,629,482 (GRCm38)	E91G	probably damaging	Het
Alms1	T	A	6: 85,622,309 (GRCm38)	N1841K	possibly damaging	Het
Arhgap25	T	A	6: 87,460,008 (GRCm38)	D620V	probably damaging	Het
Caps2	C	T	10: 112,199,727 (GRCm38)	T371I	possibly damaging	Het
Ccdc175	A	G	12: 72,140,020 (GRCm38)	I350T	possibly damaging	Het
Cdc25c	G	C	18: 34,738,239 (GRCm38)	L275V	probably damaging	Het
Cdc42bpb	A	G	12: 111,299,196 (GRCm38)	L1434P	probably damaging	Het
Cdkl3	A	T	11: 52,026,839 (GRCm38)	E321V	probably damaging	Het
Clec2d	G	T	6: 129,183,190 (GRCm38)	V56L	possibly damaging	Het
Cops3	A	T	11: 59,824,310 (GRCm38)	S301T	possibly damaging	Het
Crygd	T	C	1: 65,063,246 (GRCm38)	D19G	probably damaging	Het
Dnah2	T	C	11: 69,496,606 (GRCm38)	I931M	possibly damaging	Het
Dst	A	T	1: 34,211,170 (GRCm38)	R4068S	probably damaging	Het
Fas	C	T	19: 34,320,553 (GRCm38)		probably benign	Het
G6pd2	T	A	5: 61,810,251 (GRCm38)	D456E	probably damaging	Het
Galnt18	G	A	7: 111,554,602 (GRCm38)	R272W	probably damaging	Het
Grb2	C	A	11: 115,645,825 (GRCm38)	G200W	probably damaging	Het
Herc4	A	G	10: 63,264,053 (GRCm38)	N85S	probably benign	Het
Ighv7-2	T	C	12: 113,912,107 (GRCm38)	D92G	probably damaging	Het
Itih3	A	G	14: 30,909,835 (GRCm38)	V765A	possibly damaging	Het
Itm2b	T	C	14: 73,363,120 (GRCm38)	N247D	probably benign	Het
Kcnd3	T	C	3: 105,666,999 (GRCm38)	V500A	probably benign	Het
Lrp2	C	T	2: 69,507,843 (GRCm38)	G1198R	probably damaging	Het
Ltb4r2	A	G	14: 55,761,987 (GRCm38)	T22A	probably damaging	Het
Mdga2	A	G	12: 66,655,362 (GRCm38)	V355A	probably damaging	Het
Megf8	T	A	7: 25,353,738 (GRCm38)	V1778E	probably benign	Het
Mroh2b	G	A	15: 4,944,966 (GRCm38)	E1143K	probably damaging	Het
Nbn	A	T	4: 15,979,389 (GRCm38)	Y458F	probably damaging	Het
Nlrc3	A	G	16: 3,963,992 (GRCm38)	C534R	probably benign	Het
Nup155	A	G	15: 8,143,026 (GRCm38)	E832G	probably damaging	Het
Olfr1131	A	G	2: 87,628,829 (GRCm38)	Y122C	probably damaging	Het
Plcl2	A	G	17: 50,606,829 (GRCm38)	T289A	probably benign	Het
Ptprs	C	T	17: 56,434,990 (GRCm38)	R7Q	probably null	Het
Rab3ip	A	T	10: 116,918,960 (GRCm38)	D198E	possibly damaging	Het
Scaf4	A	T	16: 90,252,435 (GRCm38)	F255I	unknown	Het
Senp6	T	C	9: 80,126,155 (GRCm38)	S475P	probably benign	Het
Shpk	T	C	11: 73,203,959 (GRCm38)	L67P	probably damaging	Het
Sik3	T	A	9: 46,219,503 (GRCm38)	Y1246N	probably damaging	Het
Slc44a2	A	G	9: 21,353,724 (GRCm38)	Y686C	probably damaging	Het
Slc6a19	A	G	13: 73,700,566 (GRCm38)	V23A	probably benign	Het
Slit3	A	T	11: 35,544,748 (GRCm38)	I169F	possibly damaging	Het
Stxbp5l	A	G	16: 37,236,275 (GRCm38)	V379A	possibly damaging	Het
Tex2	T	C	11: 106,506,864 (GRCm38)		probably null	Het
Tnpo3	A	G	6: 29,586,144 (GRCm38)	V149A	possibly damaging	Het
Vmn2r24	T	A	6: 123,815,399 (GRCm38)	C562S	probably damaging	Het
Wipi1	T	C	11: 109,577,664 (GRCm38)	N368S	probably benign	Het
Zbtb41	T	C	1: 139,424,093 (GRCm38)	S315P	probably damaging	Het

Other mutations in Vmn1r158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01147:Vmn1r158	APN	7	22,790,779 (GRCm38)	missense	probably benign	0.01
R1173:Vmn1r158	UTSW	7	22,790,445 (GRCm38)	missense	probably benign	0.19
R1574:Vmn1r158	UTSW	7	22,790,347 (GRCm38)	missense	probably damaging	1.00
R1574:Vmn1r158	UTSW	7	22,790,347 (GRCm38)	missense	probably damaging	1.00
R1725:Vmn1r158	UTSW	7	22,790,647 (GRCm38)	missense	probably benign	0.08
R1777:Vmn1r158	UTSW	7	22,790,430 (GRCm38)	missense	probably damaging	1.00
R1813:Vmn1r158	UTSW	7	22,790,718 (GRCm38)	missense	probably damaging	1.00
R1896:Vmn1r158	UTSW	7	22,790,718 (GRCm38)	missense	probably damaging	1.00
R3749:Vmn1r158	UTSW	7	22,790,214 (GRCm38)	missense	probably damaging	1.00
R4872:Vmn1r158	UTSW	7	22,790,754 (GRCm38)	missense	possibly damaging	0.94
R5238:Vmn1r158	UTSW	7	22,790,374 (GRCm38)	missense	probably benign
R6500:Vmn1r158	UTSW	7	22,790,653 (GRCm38)	missense	possibly damaging	0.89
R6511:Vmn1r158	UTSW	7	22,790,691 (GRCm38)	missense	probably benign	0.00
R6581:Vmn1r158	UTSW	7	22,790,040 (GRCm38)	missense	possibly damaging	0.69
R6751:Vmn1r158	UTSW	7	22,789,881 (GRCm38)	missense	probably damaging	0.96
R7168:Vmn1r158	UTSW	7	22,790,676 (GRCm38)	missense	possibly damaging	0.82
R7337:Vmn1r158	UTSW	7	22,790,224 (GRCm38)	missense	probably benign	0.00
R7747:Vmn1r158	UTSW	7	22,790,300 (GRCm38)	missense	probably benign	0.15
R7902:Vmn1r158	UTSW	7	22,790,008 (GRCm38)	missense	possibly damaging	0.67
R8328:Vmn1r158	UTSW	7	22,790,062 (GRCm38)	missense	probably damaging	1.00
R8400:Vmn1r158	UTSW	7	22,789,880 (GRCm38)	nonsense	probably null
R8809:Vmn1r158	UTSW	7	22,790,350 (GRCm38)	missense	probably damaging	0.97
R9227:Vmn1r158	UTSW	7	22,790,044 (GRCm38)	missense	probably benign	0.02
R9468:Vmn1r158	UTSW	7	22,790,463 (GRCm38)	missense	probably damaging	1.00
R9702:Vmn1r158	UTSW	7	22,790,640 (GRCm38)	missense	probably benign	0.00
R9719:Vmn1r158	UTSW	7	22,789,906 (GRCm38)	missense	possibly damaging	0.95
Z1177:Vmn1r158	UTSW	7	22,790,458 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- AACTGCAAGAGGACAGTGCC -3'
(R):5'- ACAGGTGATTTTAAGCCACATG -3'

Sequencing Primer
(F):5'- GTGCCTACACTGAATCCAGAAGTG -3'
(R):5'- TTCCAAACAACATGATGGCTTTTGC -3'

Posted On

2014-09-17