Mutagenetix > Incidental Mutation

Incidental Mutation 'R2079:Lama2'

229366

Institutional Source

Beutler Lab

Gene Symbol

Lama2

Ensembl Gene

ENSMUSG00000019899

Gene Name

laminin, alpha 2

Synonyms

mer, merosin

MMRRC Submission

040084-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.320) question?

Stock #

R2079 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

26857281-27493021 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 27245049 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Serine at position 244 (I244S)

Ref Sequence

ENSEMBL: ENSMUSP00000140716 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092639] [ENSMUST00000189575]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000092639
AA Change: I244S

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000090304
Gene: ENSMUSG00000019899
AA Change: I244S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LamNT	29	281	5.35e-129	SMART
EGF_Lam	283	337	2.11e-4	SMART
EGF_Lam	340	407	1.59e-8	SMART
EGF_Lam	410	462	5.44e-7	SMART
EGF_Lam	465	511	9.05e-4	SMART
LamB	574	706	2.26e-44	SMART
Pfam:Laminin_EGF	715	745	2.8e-4	PFAM
EGF_Lam	753	800	4.03e-10	SMART
EGF_Lam	803	858	3.01e-9	SMART
EGF_Lam	861	911	1.35e-11	SMART
EGF_Lam	914	960	7.23e-12	SMART
EGF_Lam	963	1007	5.87e-12	SMART
EGF_Lam	1010	1053	1.28e-12	SMART
EGF_Lam	1056	1099	2.37e-7	SMART
EGF_Lam	1102	1159	3.22e-9	SMART
LamB	1225	1360	1.95e-57	SMART
EGF_like	1364	1413	8.13e-1	SMART
EGF_Lam	1416	1462	5.48e-12	SMART
EGF_Lam	1465	1520	1.27e-7	SMART
EGF_Lam	1523	1567	2.4e-8	SMART
Pfam:Laminin_I	1584	1849	2e-92	PFAM
Blast:MA	1881	2113	1e-112	BLAST
LamG	2162	2307	1.28e-25	SMART
LamG	2356	2500	2.2e-33	SMART
LamG	2542	2688	3.31e-28	SMART
low complexity region	2725	2741	N/A	INTRINSIC
LamG	2781	2914	2.25e-39	SMART
LamG	2956	3092	1.53e-32	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185839

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186965

Predicted Effect

probably damaging
Transcript: ENSMUST00000189575
AA Change: I244S

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000140716
Gene: ENSMUSG00000019899
AA Change: I244S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
LamNT	29	281	2.5e-131	SMART
EGF_Lam	283	337	1e-6	SMART
EGF_Lam	340	407	7.7e-11	SMART
EGF_Lam	410	462	2.6e-9	SMART
EGF_Lam	465	511	4.5e-6	SMART
LamB	574	706	1.4e-46	SMART
Pfam:Laminin_EGF	715	745	1.7e-2	PFAM
EGF_Lam	753	800	1.9e-12	SMART
EGF_Lam	803	858	1.4e-11	SMART
EGF_Lam	861	911	6.7e-14	SMART
EGF_Lam	914	960	3.6e-14	SMART
EGF_Lam	963	1007	2.9e-14	SMART
EGF_Lam	1010	1053	6.1e-15	SMART
EGF_Lam	1056	1099	1.1e-9	SMART
EGF_Lam	1102	1159	1.5e-11	SMART
LamB	1225	1349	1.4e-45	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted and spontaneous mutations exhibit progressive growth retardation, ataxia, muscle atrophy and degeneration, infertility, and premature lethality. Muscle fiber degeneration is evident as early as the first week of life. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts6	A	T	13: 104,598,746 (GRCm39)	R862S	probably benign	Het
Aldoa	T	C	7: 126,396,076 (GRCm39)	D164G	probably null	Het
Ankle2	T	C	5: 110,392,371 (GRCm39)	V459A	probably damaging	Het
Atp11a	A	G	8: 12,907,902 (GRCm39)	Y482C	probably damaging	Het
BC035947	A	G	1: 78,488,561 (GRCm39)		probably benign	Het
Cfap65	G	A	1: 74,956,358 (GRCm39)	R1074C	probably benign	Het
Ciart	G	T	3: 95,786,350 (GRCm39)	H242N	probably damaging	Het
Cidec	T	A	6: 113,402,615 (GRCm39)	M220L	probably benign	Het
Clca3a1	A	G	3: 144,713,534 (GRCm39)	I699T	possibly damaging	Het
Csf2rb2	T	C	15: 78,172,207 (GRCm39)	D401G	probably benign	Het
Cyp2j6	C	A	4: 96,419,962 (GRCm39)	L256F	possibly damaging	Het
Ddr2	A	T	1: 169,832,345 (GRCm39)	Y148*	probably null	Het
Depdc5	T	A	5: 33,104,018 (GRCm39)	I373N	possibly damaging	Het
Dpp10	A	T	1: 123,360,721 (GRCm39)	M268K	probably damaging	Het
Fam118b	C	A	9: 35,134,960 (GRCm39)	V216F	possibly damaging	Het
Fancd2	T	C	6: 113,532,148 (GRCm39)	V487A	probably damaging	Het
Fhad1	T	A	4: 141,718,513 (GRCm39)	R147*	probably null	Het
Flt3	A	G	5: 147,291,893 (GRCm39)	S544P	probably damaging	Het
Frem3	C	A	8: 81,341,732 (GRCm39)	Q1342K	probably benign	Het
Garin3	T	G	11: 46,295,934 (GRCm39)	V102G	probably benign	Het
Gimap4	T	C	6: 48,667,881 (GRCm39)	M84T	possibly damaging	Het
Gm20481	T	C	17: 35,189,196 (GRCm39)	K569R	probably benign	Het
Gm4871	A	C	5: 144,966,741 (GRCm39)	D247E	possibly damaging	Het
Gm5141	A	T	13: 62,922,424 (GRCm39)	N248K	probably benign	Het
Gulo	A	G	14: 66,227,832 (GRCm39)	Y367H	probably damaging	Het
Hap1	T	C	11: 100,244,572 (GRCm39)	E120G	probably damaging	Het
Heatr3	T	A	8: 88,868,404 (GRCm39)	N51K	probably damaging	Het
Hipk2	T	C	6: 38,795,720 (GRCm39)	D183G	probably damaging	Het
Hnrnpll	T	A	17: 80,342,806 (GRCm39)	T439S	probably benign	Het
Hoxd3	A	C	2: 74,574,610 (GRCm39)	E85D	probably damaging	Het
Ipo8	A	T	6: 148,690,660 (GRCm39)	M694K	probably damaging	Het
Jag2	A	G	12: 112,883,997 (GRCm39)	I194T	probably damaging	Het
Jrkl	A	T	9: 13,244,864 (GRCm39)	F266I	probably damaging	Het
Kcnt1	A	G	2: 25,790,260 (GRCm39)	I436V	possibly damaging	Het
Kdm3b	A	T	18: 34,936,570 (GRCm39)	D284V	probably damaging	Het
Khdrbs2	A	G	1: 32,506,955 (GRCm39)	T200A	probably benign	Het
Kmt2c	T	C	5: 25,557,278 (GRCm39)	D1143G	possibly damaging	Het
Kremen1	G	GGGGT	11: 5,151,794 (GRCm39)		probably null	Het
Lama5	G	A	2: 179,867,301 (GRCm39)	P99S	possibly damaging	Het
Lrrc30	A	T	17: 67,938,875 (GRCm39)	L235Q	possibly damaging	Het
Man2b2	C	T	5: 36,971,716 (GRCm39)	V667M	possibly damaging	Het
Mmp2	T	A	8: 93,576,817 (GRCm39)	N77K	probably damaging	Het
Myo1a	A	G	10: 127,556,482 (GRCm39)	E1009G	probably benign	Het
Ndst1	A	G	18: 60,828,581 (GRCm39)	Y658H	probably damaging	Het
Nlrp10	A	G	7: 108,524,835 (GRCm39)	L215P	possibly damaging	Het
Nrbp1	T	C	5: 31,408,417 (GRCm39)	F526L	probably benign	Het
Or10p21	T	C	10: 128,847,898 (GRCm39)	V248A	probably damaging	Het
Or51t4	T	A	7: 102,598,702 (GRCm39)	F333L	probably benign	Het
Padi2	T	C	4: 140,660,507 (GRCm39)	L329P	probably damaging	Het
Pcdhb20	A	G	18: 37,638,224 (GRCm39)	Q250R	probably benign	Het
Pcdhb3	T	A	18: 37,436,362 (GRCm39)	L776Q	possibly damaging	Het
Pik3cb	A	T	9: 98,942,257 (GRCm39)	M700K	probably benign	Het
Pla2g6	C	T	15: 79,197,194 (GRCm39)	V127M	probably damaging	Het
Rabgef1	T	C	5: 130,219,776 (GRCm39)	S80P	probably damaging	Het
Sema3a	C	T	5: 13,501,098 (GRCm39)	T47I	possibly damaging	Het
Sik2	C	T	9: 50,818,706 (GRCm39)		probably null	Het
Sin3a	T	C	9: 56,996,807 (GRCm39)	V112A	probably benign	Het
Slc26a6	G	A	9: 108,736,257 (GRCm39)	A472T	probably damaging	Het
Syne1	A	G	10: 5,311,502 (GRCm39)	V561A	probably benign	Het
Syt16	C	T	12: 74,285,073 (GRCm39)	T422I	probably damaging	Het
Tlr11	A	T	14: 50,598,437 (GRCm39)	H141L	probably damaging	Het
Tmem252	A	T	19: 24,655,017 (GRCm39)	E131D	probably benign	Het
Trim55	T	G	3: 19,698,830 (GRCm39)	L20V	probably damaging	Het
Uqcrfs1	A	T	13: 30,725,291 (GRCm39)	V83D	probably benign	Het
Utf1	C	T	7: 139,524,808 (GRCm39)	R309*	probably null	Het
Uxs1	T	C	1: 43,804,133 (GRCm39)	T261A	probably damaging	Het
Vmn1r24	T	C	6: 57,932,655 (GRCm39)	I288V	probably benign	Het
Vmn1r80	A	T	7: 11,927,121 (GRCm39)	D77V	probably damaging	Het
Vmn2r74	T	G	7: 85,606,383 (GRCm39)	H321P	probably benign	Het
Zfp638	T	C	6: 83,930,371 (GRCm39)		probably null	Het
Zwilch	T	A	9: 64,060,856 (GRCm39)	Q332L	probably damaging	Het
Zwilch	G	T	9: 64,060,857 (GRCm39)	Q332K	probably damaging	Het

Other mutations in Lama2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Lama2	APN	10	27,064,261 (GRCm39)	missense	probably benign	0.01
IGL00467:Lama2	APN	10	27,343,193 (GRCm39)	splice site	probably benign
IGL00470:Lama2	APN	10	27,119,738 (GRCm39)	missense	probably benign	0.22
IGL00517:Lama2	APN	10	27,073,326 (GRCm39)	missense	probably benign	0.01
IGL00541:Lama2	APN	10	27,064,302 (GRCm39)	missense	probably benign	0.14
IGL00931:Lama2	APN	10	26,882,772 (GRCm39)	missense	possibly damaging	0.92
IGL00951:Lama2	APN	10	26,906,281 (GRCm39)	missense	probably benign	0.03
IGL00988:Lama2	APN	10	27,245,011 (GRCm39)	nonsense	probably null
IGL01098:Lama2	APN	10	26,907,108 (GRCm39)	missense	possibly damaging	0.66
IGL01152:Lama2	APN	10	27,084,425 (GRCm39)	missense	probably benign	0.00
IGL01293:Lama2	APN	10	27,107,632 (GRCm39)	missense	probably benign	0.38
IGL01338:Lama2	APN	10	27,064,268 (GRCm39)	missense	probably benign	0.13
IGL01609:Lama2	APN	10	27,220,417 (GRCm39)	missense	probably benign	0.03
IGL01643:Lama2	APN	10	26,946,368 (GRCm39)	splice site	probably benign
IGL01675:Lama2	APN	10	27,064,050 (GRCm39)	missense	possibly damaging	0.77
IGL01681:Lama2	APN	10	27,141,041 (GRCm39)	missense	probably benign	0.33
IGL01694:Lama2	APN	10	26,882,738 (GRCm39)	missense	possibly damaging	0.75
IGL01705:Lama2	APN	10	27,065,270 (GRCm39)	splice site	probably benign
IGL01885:Lama2	APN	10	26,981,135 (GRCm39)	nonsense	probably null
IGL01935:Lama2	APN	10	27,298,600 (GRCm39)	missense	probably damaging	0.98
IGL01994:Lama2	APN	10	27,343,199 (GRCm39)	critical splice donor site	probably null
IGL02041:Lama2	APN	10	26,860,322 (GRCm39)	missense	probably damaging	1.00
IGL02067:Lama2	APN	10	27,052,792 (GRCm39)	missense	probably benign	0.02
IGL02097:Lama2	APN	10	27,014,956 (GRCm39)	missense	probably benign	0.09
IGL02179:Lama2	APN	10	26,946,360 (GRCm39)	missense	probably benign	0.01
IGL02268:Lama2	APN	10	26,877,112 (GRCm39)	splice site	probably benign
IGL02302:Lama2	APN	10	27,088,039 (GRCm39)	missense	probably benign	0.06
IGL02363:Lama2	APN	10	27,242,062 (GRCm39)	missense	probably damaging	1.00
IGL02378:Lama2	APN	10	26,919,652 (GRCm39)	missense	probably damaging	0.99
IGL02642:Lama2	APN	10	27,343,269 (GRCm39)	missense	probably damaging	1.00
IGL02676:Lama2	APN	10	26,994,489 (GRCm39)	missense	probably benign	0.00
IGL02695:Lama2	APN	10	26,876,771 (GRCm39)	missense	probably benign
IGL02735:Lama2	APN	10	26,980,124 (GRCm39)	missense	probably damaging	1.00
IGL02794:Lama2	APN	10	26,917,227 (GRCm39)	missense	possibly damaging	0.73
IGL02823:Lama2	APN	10	26,877,141 (GRCm39)	missense	probably damaging	1.00
IGL02869:Lama2	APN	10	26,891,534 (GRCm39)	missense	probably damaging	0.99
IGL02942:Lama2	APN	10	26,917,216 (GRCm39)	missense	probably damaging	1.00
IGL03201:Lama2	APN	10	27,220,566 (GRCm39)	nonsense	probably null
IGL03268:Lama2	APN	10	27,298,649 (GRCm39)	missense	probably damaging	1.00
IGL03288:Lama2	APN	10	27,245,047 (GRCm39)	missense	probably damaging	1.00
IGL03380:Lama2	APN	10	26,926,261 (GRCm39)	missense	probably damaging	1.00
IGL03407:Lama2	APN	10	27,223,017 (GRCm39)	missense	probably damaging	1.00
cowboy	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
petri	UTSW	10	26,869,394 (GRCm39)	splice site	probably null
PIT4362001:Lama2	UTSW	10	27,245,132 (GRCm39)	missense	probably damaging	1.00
PIT4382001:Lama2	UTSW	10	27,080,901 (GRCm39)	missense	probably damaging	1.00
PIT4431001:Lama2	UTSW	10	26,977,426 (GRCm39)	missense	probably damaging	1.00
R0038:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R0038:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R0114:Lama2	UTSW	10	26,869,064 (GRCm39)	nonsense	probably null
R0142:Lama2	UTSW	10	27,063,841 (GRCm39)	missense	probably benign
R0313:Lama2	UTSW	10	26,869,394 (GRCm39)	splice site	probably null
R0376:Lama2	UTSW	10	26,891,542 (GRCm39)	missense	possibly damaging	0.68
R0412:Lama2	UTSW	10	27,066,621 (GRCm39)	missense	possibly damaging	0.58
R0472:Lama2	UTSW	10	26,866,863 (GRCm39)	missense	probably damaging	1.00
R0607:Lama2	UTSW	10	27,065,127 (GRCm39)	missense	probably benign	0.34
R0648:Lama2	UTSW	10	26,865,372 (GRCm39)	missense	probably benign	0.00
R0667:Lama2	UTSW	10	27,220,406 (GRCm39)	splice site	probably null
R0760:Lama2	UTSW	10	26,920,429 (GRCm39)	critical splice donor site	probably null
R1240:Lama2	UTSW	10	26,917,120 (GRCm39)	missense	probably damaging	1.00
R1385:Lama2	UTSW	10	27,100,039 (GRCm39)	missense	probably benign	0.11
R1433:Lama2	UTSW	10	27,063,750 (GRCm39)	missense	probably damaging	1.00
R1434:Lama2	UTSW	10	27,084,366 (GRCm39)	missense	probably damaging	1.00
R1574:Lama2	UTSW	10	27,200,750 (GRCm39)	missense	possibly damaging	0.65
R1574:Lama2	UTSW	10	27,200,750 (GRCm39)	missense	possibly damaging	0.65
R1645:Lama2	UTSW	10	27,244,981 (GRCm39)	missense	probably damaging	1.00
R1702:Lama2	UTSW	10	27,066,525 (GRCm39)	missense	probably benign
R1703:Lama2	UTSW	10	27,142,667 (GRCm39)	missense	probably damaging	1.00
R1769:Lama2	UTSW	10	27,084,403 (GRCm39)	missense	probably benign
R1769:Lama2	UTSW	10	27,084,402 (GRCm39)	missense	probably damaging	1.00
R1846:Lama2	UTSW	10	27,088,092 (GRCm39)	missense	probably damaging	1.00
R1859:Lama2	UTSW	10	26,907,078 (GRCm39)	missense	possibly damaging	0.51
R1871:Lama2	UTSW	10	26,860,490 (GRCm39)	missense	probably damaging	1.00
R1903:Lama2	UTSW	10	27,064,395 (GRCm39)	missense	probably damaging	1.00
R1906:Lama2	UTSW	10	26,932,523 (GRCm39)	critical splice donor site	probably null
R1958:Lama2	UTSW	10	26,857,594 (GRCm39)	missense	probably damaging	0.97
R1959:Lama2	UTSW	10	27,298,614 (GRCm39)	missense	probably damaging	1.00
R1977:Lama2	UTSW	10	26,866,796 (GRCm39)	splice site	probably null
R2063:Lama2	UTSW	10	27,040,922 (GRCm39)	missense	probably damaging	1.00
R2085:Lama2	UTSW	10	27,080,837 (GRCm39)	nonsense	probably null
R2125:Lama2	UTSW	10	26,920,449 (GRCm39)	nonsense	probably null
R2140:Lama2	UTSW	10	26,930,690 (GRCm39)	splice site	probably null
R2219:Lama2	UTSW	10	26,919,565 (GRCm39)	missense	probably damaging	0.99
R2259:Lama2	UTSW	10	26,907,123 (GRCm39)	missense	probably benign	0.00
R2265:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2266:Lama2	UTSW	10	26,862,793 (GRCm39)	missense	probably benign	0.02
R2267:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2268:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2269:Lama2	UTSW	10	26,868,932 (GRCm39)	missense	probably damaging	1.00
R2862:Lama2	UTSW	10	27,298,608 (GRCm39)	nonsense	probably null
R2912:Lama2	UTSW	10	26,876,799 (GRCm39)	missense	probably benign
R2999:Lama2	UTSW	10	26,865,417 (GRCm39)	missense	probably benign	0.18
R3034:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3081:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3107:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3109:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3436:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3437:Lama2	UTSW	10	26,877,231 (GRCm39)	missense	probably benign	0.11
R3706:Lama2	UTSW	10	27,014,992 (GRCm39)	missense	probably damaging	1.00
R3780:Lama2	UTSW	10	27,335,335 (GRCm39)	missense	probably damaging	1.00
R3807:Lama2	UTSW	10	27,066,661 (GRCm39)	frame shift	probably null
R3919:Lama2	UTSW	10	26,994,501 (GRCm39)	missense	probably damaging	1.00
R4014:Lama2	UTSW	10	26,860,372 (GRCm39)	missense	probably damaging	1.00
R4131:Lama2	UTSW	10	26,917,170 (GRCm39)	missense	probably benign	0.00
R4190:Lama2	UTSW	10	27,142,660 (GRCm39)	missense	probably damaging	0.96
R4273:Lama2	UTSW	10	27,223,050 (GRCm39)	missense	probably damaging	1.00
R4358:Lama2	UTSW	10	26,860,489 (GRCm39)	missense	probably damaging	1.00
R4407:Lama2	UTSW	10	27,088,124 (GRCm39)	small deletion	probably benign
R4415:Lama2	UTSW	10	26,865,340 (GRCm39)	nonsense	probably null
R4426:Lama2	UTSW	10	27,298,554 (GRCm39)	missense	probably damaging	1.00
R4590:Lama2	UTSW	10	26,865,410 (GRCm39)	missense	probably benign	0.00
R4615:Lama2	UTSW	10	26,857,520 (GRCm39)	missense	probably damaging	0.99
R4736:Lama2	UTSW	10	27,080,925 (GRCm39)	missense	probably damaging	1.00
R4754:Lama2	UTSW	10	26,994,527 (GRCm39)	missense	possibly damaging	0.58
R4791:Lama2	UTSW	10	27,343,267 (GRCm39)	missense	probably damaging	1.00
R4834:Lama2	UTSW	10	26,882,745 (GRCm39)	missense	probably benign	0.30
R4856:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4858:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4859:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4897:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4898:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4899:Lama2	UTSW	10	26,919,639 (GRCm39)	frame shift	probably null
R4907:Lama2	UTSW	10	27,040,942 (GRCm39)	missense	probably benign	0.11
R4911:Lama2	UTSW	10	27,014,923 (GRCm39)	missense	probably damaging	1.00
R4924:Lama2	UTSW	10	27,245,137 (GRCm39)	missense	probably damaging	0.98
R5023:Lama2	UTSW	10	27,066,500 (GRCm39)	missense	probably damaging	0.97
R5057:Lama2	UTSW	10	27,040,982 (GRCm39)	missense	probably damaging	1.00
R5070:Lama2	UTSW	10	27,226,247 (GRCm39)	critical splice donor site	probably null
R5116:Lama2	UTSW	10	26,994,556 (GRCm39)	missense	probably benign	0.08
R5177:Lama2	UTSW	10	27,066,699 (GRCm39)	missense	possibly damaging	0.94
R5198:Lama2	UTSW	10	27,222,999 (GRCm39)	missense	probably damaging	0.96
R5289:Lama2	UTSW	10	27,088,069 (GRCm39)	nonsense	probably null
R5327:Lama2	UTSW	10	27,014,942 (GRCm39)	missense	probably benign
R5424:Lama2	UTSW	10	26,860,392 (GRCm39)	missense	probably damaging	1.00
R5469:Lama2	UTSW	10	26,917,185 (GRCm39)	missense	possibly damaging	0.92
R5620:Lama2	UTSW	10	26,866,876 (GRCm39)	missense	probably damaging	0.99
R5667:Lama2	UTSW	10	27,066,540 (GRCm39)	missense	probably damaging	1.00
R5671:Lama2	UTSW	10	27,066,540 (GRCm39)	missense	probably damaging	1.00
R5815:Lama2	UTSW	10	26,862,847 (GRCm39)	missense	probably damaging	1.00
R5917:Lama2	UTSW	10	27,066,693 (GRCm39)	missense	probably damaging	1.00
R5935:Lama2	UTSW	10	26,891,494 (GRCm39)	missense	probably benign
R5976:Lama2	UTSW	10	27,066,672 (GRCm39)	missense	probably benign	0.00
R5979:Lama2	UTSW	10	27,111,728 (GRCm39)	missense	probably damaging	0.99
R6004:Lama2	UTSW	10	27,111,781 (GRCm39)	missense	probably benign	0.01
R6180:Lama2	UTSW	10	26,857,495 (GRCm39)	missense	probably benign	0.03
R6198:Lama2	UTSW	10	27,064,018 (GRCm39)	missense	probably damaging	1.00
R6257:Lama2	UTSW	10	26,862,895 (GRCm39)	missense	possibly damaging	0.85
R6271:Lama2	UTSW	10	26,899,325 (GRCm39)	missense	possibly damaging	0.67
R6322:Lama2	UTSW	10	27,066,543 (GRCm39)	missense	probably damaging	0.96
R6354:Lama2	UTSW	10	27,088,064 (GRCm39)	missense	probably damaging	1.00
R6431:Lama2	UTSW	10	26,929,027 (GRCm39)	missense	possibly damaging	0.50
R6499:Lama2	UTSW	10	26,907,154 (GRCm39)	missense	probably damaging	1.00
R6535:Lama2	UTSW	10	26,980,127 (GRCm39)	missense	probably damaging	1.00
R6545:Lama2	UTSW	10	27,052,793 (GRCm39)	missense	probably benign
R6636:Lama2	UTSW	10	27,000,564 (GRCm39)	missense	probably benign	0.13
R6891:Lama2	UTSW	10	27,204,078 (GRCm39)	nonsense	probably null
R6891:Lama2	UTSW	10	27,204,068 (GRCm39)	nonsense	probably null
R6902:Lama2	UTSW	10	26,857,625 (GRCm39)	missense	probably damaging	1.00
R6908:Lama2	UTSW	10	26,907,192 (GRCm39)	splice site	probably null
R7168:Lama2	UTSW	10	27,242,148 (GRCm39)	critical splice acceptor site	probably null
R7233:Lama2	UTSW	10	27,107,659 (GRCm39)	missense	probably damaging	1.00
R7272:Lama2	UTSW	10	27,000,552 (GRCm39)	missense	probably damaging	1.00
R7274:Lama2	UTSW	10	26,995,976 (GRCm39)	missense	probably damaging	0.99
R7419:Lama2	UTSW	10	27,142,630 (GRCm39)	missense	probably benign
R7423:Lama2	UTSW	10	27,088,222 (GRCm39)	missense	probably benign	0.00
R7554:Lama2	UTSW	10	27,031,492 (GRCm39)	missense	probably damaging	1.00
R7569:Lama2	UTSW	10	27,141,046 (GRCm39)	missense	probably damaging	1.00
R7574:Lama2	UTSW	10	26,882,726 (GRCm39)	missense	probably benign	0.03
R7584:Lama2	UTSW	10	26,980,257 (GRCm39)	missense	possibly damaging	0.78
R7586:Lama2	UTSW	10	26,977,389 (GRCm39)	missense	probably benign	0.00
R7603:Lama2	UTSW	10	27,142,676 (GRCm39)	missense	possibly damaging	0.55
R7691:Lama2	UTSW	10	27,084,389 (GRCm39)	missense	possibly damaging	0.67
R7750:Lama2	UTSW	10	26,866,920 (GRCm39)	missense	probably damaging	0.97
R7841:Lama2	UTSW	10	27,031,529 (GRCm39)	missense	probably benign	0.00
R7864:Lama2	UTSW	10	26,932,611 (GRCm39)	missense	probably benign	0.08
R7960:Lama2	UTSW	10	26,869,094 (GRCm39)	missense	probably benign	0.04
R7964:Lama2	UTSW	10	27,099,977 (GRCm39)	critical splice donor site	probably null
R7980:Lama2	UTSW	10	27,239,609 (GRCm39)	missense	probably damaging	0.98
R8013:Lama2	UTSW	10	27,220,494 (GRCm39)	missense	probably benign	0.00
R8028:Lama2	UTSW	10	27,204,145 (GRCm39)	missense	probably benign	0.13
R8097:Lama2	UTSW	10	27,066,660 (GRCm39)	nonsense	probably null
R8100:Lama2	UTSW	10	26,917,113 (GRCm39)	missense	probably benign	0.03
R8110:Lama2	UTSW	10	26,866,866 (GRCm39)	missense	probably damaging	1.00
R8122:Lama2	UTSW	10	26,930,592 (GRCm39)	missense	possibly damaging	0.87
R8264:Lama2	UTSW	10	27,343,218 (GRCm39)	missense	probably benign	0.07
R8315:Lama2	UTSW	10	27,298,655 (GRCm39)	missense	probably damaging	1.00
R8318:Lama2	UTSW	10	26,860,334 (GRCm39)	missense	probably damaging	1.00
R8419:Lama2	UTSW	10	27,298,559 (GRCm39)	missense	probably benign	0.26
R8475:Lama2	UTSW	10	26,977,369 (GRCm39)	missense	possibly damaging	0.69
R8735:Lama2	UTSW	10	27,066,530 (GRCm39)	missense	probably damaging	1.00
R8754:Lama2	UTSW	10	26,877,147 (GRCm39)	missense	possibly damaging	0.83
R8817:Lama2	UTSW	10	27,063,869 (GRCm39)	missense	probably damaging	1.00
R8851:Lama2	UTSW	10	27,242,119 (GRCm39)	missense	possibly damaging	0.94
R8859:Lama2	UTSW	10	27,335,384 (GRCm39)	missense	possibly damaging	0.58
R8886:Lama2	UTSW	10	27,245,157 (GRCm39)	splice site	probably benign
R8937:Lama2	UTSW	10	26,862,816 (GRCm39)	missense	probably damaging	1.00
R8993:Lama2	UTSW	10	27,298,710 (GRCm39)	missense	possibly damaging	0.91
R9025:Lama2	UTSW	10	26,860,367 (GRCm39)	missense	probably benign	0.00
R9027:Lama2	UTSW	10	27,080,881 (GRCm39)	missense	probably damaging	1.00
R9047:Lama2	UTSW	10	26,882,697 (GRCm39)	missense	possibly damaging	0.50
R9075:Lama2	UTSW	10	26,857,588 (GRCm39)	missense	probably damaging	1.00
R9135:Lama2	UTSW	10	27,298,515 (GRCm39)	missense	probably damaging	1.00
R9165:Lama2	UTSW	10	26,929,022 (GRCm39)	critical splice donor site	probably null
R9192:Lama2	UTSW	10	27,204,181 (GRCm39)	missense	possibly damaging	0.95
R9254:Lama2	UTSW	10	27,298,685 (GRCm39)	missense	probably damaging	0.96
R9326:Lama2	UTSW	10	26,906,193 (GRCm39)	missense	probably benign	0.04
R9356:Lama2	UTSW	10	27,088,186 (GRCm39)	missense	probably damaging	0.99
R9358:Lama2	UTSW	10	27,064,378 (GRCm39)	missense	possibly damaging	0.95
R9358:Lama2	UTSW	10	27,492,761 (GRCm39)	missense	unknown
R9376:Lama2	UTSW	10	26,994,620 (GRCm39)	missense	probably benign	0.11
R9381:Lama2	UTSW	10	27,064,023 (GRCm39)	nonsense	probably null
R9397:Lama2	UTSW	10	26,981,117 (GRCm39)	missense	probably benign	0.01
R9460:Lama2	UTSW	10	27,298,475 (GRCm39)	missense	probably damaging	1.00
R9478:Lama2	UTSW	10	26,891,478 (GRCm39)	missense	probably damaging	0.98
R9503:Lama2	UTSW	10	26,865,440 (GRCm39)	missense	possibly damaging	0.57
R9514:Lama2	UTSW	10	27,100,015 (GRCm39)	missense	probably benign	0.00
R9515:Lama2	UTSW	10	26,877,170 (GRCm39)	missense	probably benign	0.23
R9516:Lama2	UTSW	10	27,100,015 (GRCm39)	missense	probably benign	0.00
R9533:Lama2	UTSW	10	26,862,871 (GRCm39)	missense	probably damaging	1.00
R9619:Lama2	UTSW	10	27,064,282 (GRCm39)	missense	probably damaging	1.00
R9721:Lama2	UTSW	10	27,343,338 (GRCm39)	missense	possibly damaging	0.58

Predicted Primers

PCR Primer
(F):5'- ATCCTTGACAGACCGCTAAG -3'
(R):5'- ATGAGCAAACTGTTACATGTGCATG -3'

Sequencing Primer
(F):5'- CTAAGAAGCTGGGCACAATATG -3'
(R):5'- TGAGCACAAGCCTGCATCTG -3'

Posted On

2014-09-17