Mutagenetix > Incidental Mutation

Incidental Mutation 'R2126:Dclk2'

229855

Institutional Source

Beutler Lab

Gene Symbol

Dclk2

Ensembl Gene

ENSMUSG00000028078

Gene Name

doublecortin-like kinase 2

Synonyms

Dcamkl2, 6330415M09Rik, Click-II

MMRRC Submission

040129-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2126 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86693458-86828159 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 86712946 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 503 (R503Q)

Ref Sequence

ENSEMBL: ENSMUSP00000141707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029719] [ENSMUST00000191752] [ENSMUST00000192773] [ENSMUST00000193632] [ENSMUST00000194452] [ENSMUST00000195561]

AlphaFold

Q6PGN3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000029719
AA Change: R503Q

PolyPhen 2 Score 0.500 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000029719
Gene: ENSMUSG00000028078
AA Change: R503Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	2.29e-42	SMART
DCX	191	279	2.17e-34	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	323	346	N/A	INTRINSIC
S_TKc	393	650	4.96e-101	SMART
low complexity region	718	740	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000191752
AA Change: R503Q

PolyPhen 2 Score 0.500 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000141707
Gene: ENSMUSG00000028078
AA Change: R503Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	2.29e-42	SMART
DCX	191	279	2.17e-34	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	323	346	N/A	INTRINSIC
S_TKc	393	646	2.4e-76	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000192773
AA Change: R502Q

PolyPhen 2 Score 0.042 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000141567
Gene: ENSMUSG00000028078
AA Change: R502Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	1.1e-44	SMART
DCX	191	279	9.9e-37	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	323	346	N/A	INTRINSIC
S_TKc	392	641	8.6e-81	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000193632
AA Change: R519Q

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000141866
Gene: ENSMUSG00000028078
AA Change: R519Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	1.1e-44	SMART
DCX	191	279	9.9e-37	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	339	362	N/A	INTRINSIC
S_TKc	409	666	2.4e-103	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000194452
AA Change: R502Q

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000141816
Gene: ENSMUSG00000028078
AA Change: R502Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	2.29e-42	SMART
DCX	191	279	2.17e-34	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	323	346	N/A	INTRINSIC
Pfam:Pkinase_Tyr	392	590	2.2e-31	PFAM
Pfam:Pkinase	392	591	4.7e-59	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000195561
AA Change: R502Q

PolyPhen 2 Score 0.208 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000142267
Gene: ENSMUSG00000028078
AA Change: R502Q

Domain	Start	End	E-Value	Type
low complexity region	18	43	N/A	INTRINSIC
DCX	67	158	2.29e-42	SMART
DCX	191	279	2.17e-34	SMART
low complexity region	291	317	N/A	INTRINSIC
low complexity region	323	346	N/A	INTRINSIC
S_TKc	392	649	4.96e-101	SMART
low complexity region	717	739	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(59) : Targeted, knock-out(1) Gene trapped(58)

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadac	A	G	3: 59,947,066 (GRCm39)	T255A	possibly damaging	Het
Acat3	C	T	17: 13,146,294 (GRCm39)	A230T	probably benign	Het
Acsl1	C	A	8: 46,986,663 (GRCm39)	P650Q	probably benign	Het
Adgrl3	T	A	5: 81,660,383 (GRCm39)	I316N	probably damaging	Het
Agrp	G	T	8: 106,293,467 (GRCm39)	T106K	probably damaging	Het
AI429214	T	A	8: 37,461,362 (GRCm39)	V170E	probably benign	Het
Akap13	G	A	7: 75,375,052 (GRCm39)	G1895S	possibly damaging	Het
Alpk2	G	A	18: 65,483,439 (GRCm39)	Q190*	probably null	Het
Aox3	C	A	1: 58,197,375 (GRCm39)	Q574K	probably benign	Het
Apeh	A	G	9: 107,962,866 (GRCm39)	Y702H	probably damaging	Het
Aqp11	A	G	7: 97,386,692 (GRCm39)	I151T	probably benign	Het
Arhgap28	A	G	17: 68,176,010 (GRCm39)	V363A	possibly damaging	Het
Arhgef18	A	G	8: 3,501,939 (GRCm39)	N699S	probably damaging	Het
Asnsd1	A	G	1: 53,386,476 (GRCm39)	S384P	probably benign	Het
Atl1	G	T	12: 69,978,431 (GRCm39)		probably null	Het
Atp13a2	A	T	4: 140,722,702 (GRCm39)	D203V	possibly damaging	Het
Axdnd1	A	T	1: 156,160,784 (GRCm39)	N164K	probably benign	Het
Bnipl	T	A	3: 95,152,994 (GRCm39)	I162F	probably damaging	Het
Cacna1d	A	T	14: 29,845,120 (GRCm39)	L655I	probably damaging	Het
Canx	T	C	11: 50,195,185 (GRCm39)	I294M	probably damaging	Het
Casz1	T	C	4: 149,030,521 (GRCm39)	F1180S	probably damaging	Het
Cav3	T	C	6: 112,449,344 (GRCm39)	Y121H	probably benign	Het
Cd4	A	C	6: 124,847,499 (GRCm39)	S222A	probably benign	Het
Cds1	T	C	5: 101,960,416 (GRCm39)	I289T	probably benign	Het
Cep112	T	C	11: 108,399,084 (GRCm39)	F328L	probably damaging	Het
Ces4a	G	A	8: 105,864,729 (GRCm39)	G69S	probably damaging	Het
Cfap44	A	T	16: 44,230,838 (GRCm39)	D273V	probably benign	Het
Cfap91	T	C	16: 38,162,124 (GRCm39)	T6A	probably benign	Het
Clec7a	C	T	6: 129,447,918 (GRCm39)	G49D	probably benign	Het
Col22a1	G	A	15: 71,729,102 (GRCm39)	Q599*	probably null	Het
Col6a5	T	A	9: 105,822,799 (GRCm39)	H186L	unknown	Het
Dnah12	A	T	14: 26,445,613 (GRCm39)	R725*	probably null	Het
Dnajc10	G	A	2: 80,181,078 (GRCm39)		probably null	Het
Edem3	A	T	1: 151,670,482 (GRCm39)	H337L	possibly damaging	Het
Eif2ak4	A	T	2: 118,252,604 (GRCm39)	H392L	probably benign	Het
Ercc6l2	G	A	13: 63,996,585 (GRCm39)	V365I	probably damaging	Het
Fan1	T	C	7: 63,996,636 (GRCm39)	E978G	probably damaging	Het
Fcrl6	C	T	1: 172,426,815 (GRCm39)	V44M	probably benign	Het
Gaa	G	A	11: 119,161,108 (GRCm39)	W50*	probably null	Het
Gm10032	T	C	14: 67,030,227 (GRCm39)		noncoding transcript	Het
Gm10985	CTCTAT	CT	3: 53,752,670 (GRCm39)		probably null	Het
Gprc5b	G	T	7: 118,583,398 (GRCm39)	P157Q	probably damaging	Het
Gsdmc3	G	A	15: 63,730,383 (GRCm39)	Q394*	probably null	Het
Gucd1	A	G	10: 75,347,922 (GRCm39)	S38P	probably damaging	Het
Higd1a	A	T	9: 121,679,313 (GRCm39)	I58N	probably damaging	Het
Hmx3	G	C	7: 131,146,278 (GRCm39)	V329L	possibly damaging	Het
Hnrnpul2	A	T	19: 8,801,802 (GRCm39)	R337*	probably null	Het
Idua	T	A	5: 108,829,304 (GRCm39)	H368Q	possibly damaging	Het
Ifih1	A	G	2: 62,453,811 (GRCm39)	V218A	probably benign	Het
Ip6k1	G	A	9: 107,918,195 (GRCm39)	E77K	possibly damaging	Het
Kif1b	G	A	4: 149,272,097 (GRCm39)	S1568L	possibly damaging	Het
Klhl30	T	A	1: 91,286,499 (GRCm39)		probably null	Het
Lasp1	T	A	11: 97,726,960 (GRCm39)	D227E	probably benign	Het
Lhx6	A	G	2: 35,981,336 (GRCm39)	I85T	possibly damaging	Het
Limch1	A	G	5: 67,187,103 (GRCm39)	D840G	probably damaging	Het
Loxl4	C	G	19: 42,592,402 (GRCm39)	E385D	probably damaging	Het
Lrrtm4	A	T	6: 79,998,722 (GRCm39)	I44F	probably damaging	Het
Ltbp2	T	C	12: 84,832,483 (GRCm39)		probably null	Het
Lyg1	T	C	1: 37,989,755 (GRCm39)	Y44C	probably damaging	Het
Map1a	A	G	2: 121,129,122 (GRCm39)	I129V	probably damaging	Het
Med27	C	T	2: 29,414,442 (GRCm39)	Q150*	probably null	Het
Ms4a5	A	T	19: 11,256,732 (GRCm39)	I55N	probably damaging	Het
Muc5ac	T	C	7: 141,364,479 (GRCm39)	S2597P	possibly damaging	Het
Mxd1	A	C	6: 86,628,422 (GRCm39)		probably null	Het
Myo3a	A	G	2: 22,468,186 (GRCm39)	D480G	probably benign	Het
Neb	T	C	2: 52,200,650 (GRCm39)	Y343C	probably damaging	Het
Niban1	A	C	1: 151,571,886 (GRCm39)	E277A	probably damaging	Het
Niban1	A	G	1: 151,584,884 (GRCm39)	I494V	possibly damaging	Het
Nrn1	A	C	13: 36,914,180 (GRCm39)	V34G	probably damaging	Het
Or4k52	A	G	2: 111,610,841 (GRCm39)	M59V	probably damaging	Het
Or4n5	A	G	14: 50,132,524 (GRCm39)	I245T	probably benign	Het
Or52b1	C	T	7: 104,978,459 (GRCm39)	W313*	probably null	Het
Or56a3	A	G	7: 104,735,822 (GRCm39)	T300A	probably damaging	Het
Or8b39	G	T	9: 37,996,572 (GRCm39)	V147L	probably benign	Het
Or8h9	T	C	2: 86,789,442 (GRCm39)	Y120C	possibly damaging	Het
Or8k40	A	T	2: 86,584,796 (GRCm39)	N95K	probably benign	Het
Pdia4	A	T	6: 47,773,771 (GRCm39)	V526E	probably damaging	Het
Pdia6	T	C	12: 17,328,546 (GRCm39)	V167A	probably damaging	Het
Pgk2	A	G	17: 40,518,400 (GRCm39)	F343L	probably damaging	Het
Piwil1	T	C	5: 128,831,160 (GRCm39)	V827A	probably damaging	Het
Plag1	A	T	4: 3,904,169 (GRCm39)	Y341N	possibly damaging	Het
Plch2	T	C	4: 155,083,456 (GRCm39)	E393G	probably damaging	Het
Ptprg	T	A	14: 12,154,355 (GRCm38)	M692K	probably benign	Het
Rassf8	G	A	6: 145,760,908 (GRCm39)	R78H	probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnf213	A	G	11: 119,341,027 (GRCm39)	Q3556R	probably damaging	Het
Rpa2	T	C	4: 132,496,099 (GRCm39)		probably null	Het
Rpe	T	G	1: 66,755,139 (GRCm39)	F174V	possibly damaging	Het
Sbf2	T	C	7: 110,159,502 (GRCm39)	D36G	probably damaging	Het
Scn2a	T	A	2: 65,582,423 (GRCm39)	Y1590*	probably null	Het
Slc24a4	T	C	12: 102,189,018 (GRCm39)	V151A	probably damaging	Het
Slc30a8	A	T	15: 52,159,330 (GRCm39)	M17L	probably benign	Het
Slit3	T	C	11: 35,579,506 (GRCm39)	Y1228H	probably damaging	Het
Smad4	G	A	18: 73,795,815 (GRCm39)	T193M	probably benign	Het
Smtn	T	A	11: 3,480,045 (GRCm39)	H392L	probably benign	Het
St8sia3	A	G	18: 64,402,745 (GRCm39)	D128G	probably damaging	Het
Sucla2	A	T	14: 73,830,108 (GRCm39)	M382L	possibly damaging	Het
Tbx5	A	G	5: 119,974,988 (GRCm39)	T4A	probably benign	Het
Tdrd5	T	C	1: 156,104,143 (GRCm39)	R528G	probably damaging	Het
Tex44	T	C	1: 86,354,811 (GRCm39)	L240P	probably benign	Het
Tns4	A	T	11: 98,970,904 (GRCm39)		probably null	Het
Trappc10	A	T	10: 78,039,758 (GRCm39)	V731E	possibly damaging	Het
Ttf1	A	G	2: 28,961,357 (GRCm39)	K582E	probably damaging	Het
Ttf2	C	A	3: 100,855,509 (GRCm39)	Q895H	possibly damaging	Het
Ttll6	A	G	11: 96,038,358 (GRCm39)	E402G	probably damaging	Het
Ttn	A	T	2: 76,720,436 (GRCm39)		probably null	Het
Ugt8a	T	C	3: 125,669,195 (GRCm39)	D303G	probably damaging	Het
Ulk1	G	T	5: 110,940,302 (GRCm39)	A373D	probably benign	Het
Vmn2r13	A	G	5: 109,306,058 (GRCm39)	S507P	probably benign	Het
Vmn2r19	T	A	6: 123,293,033 (GRCm39)	D358E	possibly damaging	Het
Vmn2r88	T	C	14: 51,651,264 (GRCm39)	S201P	probably benign	Het
Zfp36l2	A	G	17: 84,494,403 (GRCm39)	F78S	probably damaging	Het
Zfp454	A	C	11: 50,764,822 (GRCm39)	S203R	probably benign	Het
Zfp616	C	A	11: 73,976,229 (GRCm39)	Q833K	probably benign	Het
Znhit2	A	G	19: 6,112,091 (GRCm39)	T279A	probably benign	Het
Zswim3	T	A	2: 164,661,913 (GRCm39)	I131N	probably benign	Het

Other mutations in Dclk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Dclk2	APN	3	86,706,397 (GRCm39)	critical splice acceptor site	probably null
IGL01769:Dclk2	APN	3	86,723,667 (GRCm39)	missense	possibly damaging	0.50
IGL01802:Dclk2	APN	3	86,706,334 (GRCm39)	missense	probably damaging	1.00
IGL02296:Dclk2	APN	3	86,700,600 (GRCm39)	missense	probably damaging	1.00
IGL02390:Dclk2	APN	3	86,731,990 (GRCm39)	missense	probably damaging	0.99
IGL02522:Dclk2	APN	3	86,827,423 (GRCm39)	missense	probably benign	0.01
IGL03104:Dclk2	APN	3	86,743,666 (GRCm39)	missense	probably damaging	1.00
IGL03337:Dclk2	APN	3	86,813,366 (GRCm39)	missense	probably damaging	1.00
R0219:Dclk2	UTSW	3	86,720,976 (GRCm39)	splice site	probably benign
R0400:Dclk2	UTSW	3	86,721,054 (GRCm39)	splice site	probably null
R0606:Dclk2	UTSW	3	86,813,311 (GRCm39)	missense	probably damaging	1.00
R1537:Dclk2	UTSW	3	86,713,491 (GRCm39)	missense	probably damaging	0.97
R1569:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1571:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1612:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1680:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1689:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1714:Dclk2	UTSW	3	86,813,400 (GRCm39)	missense	probably benign	0.00
R1745:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1746:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R1752:Dclk2	UTSW	3	86,713,434 (GRCm39)	missense	possibly damaging	0.61
R1829:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R2008:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R2125:Dclk2	UTSW	3	86,712,946 (GRCm39)	missense	possibly damaging	0.50
R2132:Dclk2	UTSW	3	86,827,353 (GRCm39)	missense	probably benign	0.44
R2314:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R2338:Dclk2	UTSW	3	86,706,324 (GRCm39)	missense	probably damaging	1.00
R2849:Dclk2	UTSW	3	86,700,530 (GRCm39)	missense	probably damaging	1.00
R3108:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R3109:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R3615:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R3616:Dclk2	UTSW	3	86,827,342 (GRCm39)	missense	probably damaging	1.00
R4051:Dclk2	UTSW	3	86,738,129 (GRCm39)	critical splice donor site	probably null
R4052:Dclk2	UTSW	3	86,738,129 (GRCm39)	critical splice donor site	probably null
R4208:Dclk2	UTSW	3	86,738,129 (GRCm39)	critical splice donor site	probably null
R4643:Dclk2	UTSW	3	86,713,487 (GRCm39)	missense	possibly damaging	0.93
R4654:Dclk2	UTSW	3	86,743,683 (GRCm39)	missense	probably damaging	1.00
R4693:Dclk2	UTSW	3	86,722,400 (GRCm39)	missense	possibly damaging	0.67
R4716:Dclk2	UTSW	3	86,827,188 (GRCm39)	missense	probably damaging	1.00
R4914:Dclk2	UTSW	3	86,732,049 (GRCm39)	splice site	probably null
R4915:Dclk2	UTSW	3	86,732,049 (GRCm39)	splice site	probably null
R4917:Dclk2	UTSW	3	86,732,049 (GRCm39)	splice site	probably null
R5218:Dclk2	UTSW	3	86,712,985 (GRCm39)	missense	probably damaging	1.00
R5510:Dclk2	UTSW	3	86,813,344 (GRCm39)	missense	possibly damaging	0.93
R5520:Dclk2	UTSW	3	86,827,147 (GRCm39)	missense	probably damaging	1.00
R5867:Dclk2	UTSW	3	86,699,166 (GRCm39)	makesense	probably null
R5976:Dclk2	UTSW	3	86,694,532 (GRCm39)	missense	possibly damaging	0.53
R6048:Dclk2	UTSW	3	86,813,272 (GRCm39)	missense	probably damaging	1.00
R6111:Dclk2	UTSW	3	86,712,968 (GRCm39)	missense	probably benign	0.28
R6192:Dclk2	UTSW	3	86,722,457 (GRCm39)	missense	probably damaging	1.00
R6289:Dclk2	UTSW	3	86,739,124 (GRCm39)	missense	probably benign	0.18
R6595:Dclk2	UTSW	3	86,699,374 (GRCm39)	critical splice donor site	probably benign
R6897:Dclk2	UTSW	3	86,739,070 (GRCm39)	missense	probably benign	0.00
R7061:Dclk2	UTSW	3	86,739,038 (GRCm39)	critical splice donor site	probably null
R7095:Dclk2	UTSW	3	86,700,566 (GRCm39)	missense	probably damaging	1.00
R7096:Dclk2	UTSW	3	86,700,566 (GRCm39)	missense	probably damaging	1.00
R7208:Dclk2	UTSW	3	86,706,909 (GRCm39)	splice site	probably null
R7253:Dclk2	UTSW	3	86,700,566 (GRCm39)	missense	probably damaging	1.00
R7256:Dclk2	UTSW	3	86,700,566 (GRCm39)	missense	probably damaging	1.00
R8003:Dclk2	UTSW	3	86,700,608 (GRCm39)	critical splice acceptor site	probably null
R8061:Dclk2	UTSW	3	86,720,981 (GRCm39)	splice site	probably benign
R8927:Dclk2	UTSW	3	86,739,048 (GRCm39)	missense	probably damaging	1.00
R8928:Dclk2	UTSW	3	86,739,048 (GRCm39)	missense	probably damaging	1.00
R8964:Dclk2	UTSW	3	86,743,698 (GRCm39)	missense	probably damaging	1.00
R9704:Dclk2	UTSW	3	86,827,387 (GRCm39)	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- GTGTGCATGCAAAACACCCC -3'
(R):5'- TTCTGCAAACTGATGTGACACCC -3'

Sequencing Primer
(F):5'- ACCAACTTGAACTTGCTGGC -3'
(R):5'- CTTCTTATTTCAGAGGACCGAACTG -3'

Posted On

2014-09-17