Mutagenetix > Incidental Mutation

Incidental Mutation 'R2126:Akap13'

229892

Institutional Source

Beutler Lab

Gene Symbol

Akap13

Ensembl Gene

ENSMUSG00000066406

Gene Name

A kinase (PRKA) anchor protein 13

Synonyms

AKAP-Lbc, 5730522G15Rik, 1700026G02Rik, PROTO-LBC, PROTO-LB, Ht31, 5830460E08Rik

MMRRC Submission

040129-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2126 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

75455534-75754609 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 75725304 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 1895 (G1895S)

Ref Sequence

ENSEMBL: ENSMUSP00000147237 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166315] [ENSMUST00000207239] [ENSMUST00000207750]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000147005
AA Change: G1895S

PolyPhen 2 Score 0.484 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000117686
Gene: ENSMUSG00000066406
AA Change: G1895S

Domain	Start	End	E-Value	Type
low complexity region	174	184	N/A	INTRINSIC
internal_repeat_1	485	695	4.85e-5	PROSPERO
low complexity region	773	789	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	1021	1032	N/A	INTRINSIC
Pfam:RII_binding_1	1218	1235	4.3e-6	PFAM
low complexity region	1429	1444	N/A	INTRINSIC
low complexity region	1479	1501	N/A	INTRINSIC
low complexity region	1601	1612	N/A	INTRINSIC
low complexity region	1738	1768	N/A	INTRINSIC
C1	1773	1819	1.95e-4	SMART
low complexity region	1876	1887	N/A	INTRINSIC
RhoGEF	1979	2171	1.28e-61	SMART
PH	2213	2316	2.94e-11	SMART
coiled coil region	2326	2363	N/A	INTRINSIC
low complexity region	2411	2430	N/A	INTRINSIC
low complexity region	2462	2472	N/A	INTRINSIC
coiled coil region	2551	2664	N/A	INTRINSIC
low complexity region	2746	2752	N/A	INTRINSIC
low complexity region	2758	2771	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000166315
AA Change: G1877S

PolyPhen 2 Score 0.950 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000129784
Gene: ENSMUSG00000066406
AA Change: G1877S

Domain	Start	End	E-Value	Type
low complexity region	174	184	N/A	INTRINSIC
low complexity region	773	789	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	1021	1032	N/A	INTRINSIC
low complexity region	1433	1448	N/A	INTRINSIC
low complexity region	1483	1505	N/A	INTRINSIC
low complexity region	1583	1594	N/A	INTRINSIC
low complexity region	1720	1750	N/A	INTRINSIC
C1	1755	1801	1.95e-4	SMART
low complexity region	1858	1869	N/A	INTRINSIC
RhoGEF	1961	2153	1.28e-61	SMART
PH	2195	2298	2.94e-11	SMART
coiled coil region	2308	2345	N/A	INTRINSIC
low complexity region	2393	2412	N/A	INTRINSIC
low complexity region	2444	2454	N/A	INTRINSIC
coiled coil region	2533	2646	N/A	INTRINSIC
low complexity region	2728	2734	N/A	INTRINSIC
low complexity region	2740	2753	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000207239
AA Change: G176S

PolyPhen 2 Score 0.323 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000207750
AA Change: G1895S

PolyPhen 2 Score 0.950 (Sensitivity: 0.79; Specificity: 0.95)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. [provided by RefSeq, Jul 2012]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality during organogenesis, arrested heart development, and forebrain hypoplasia. Heterozygous mice exhibit small spleen, impaired lymphocyte response to osmotic stress, decreased response to glucocorticoid, osteoporosis and impared osteogenesis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadac	A	G	3: 60,039,645 (GRCm38)	T255A	possibly damaging	Het
Acat3	C	T	17: 12,927,407 (GRCm38)	A230T	probably benign	Het
Acsl1	C	A	8: 46,533,626 (GRCm38)	P650Q	probably benign	Het
Adgrl3	T	A	5: 81,512,536 (GRCm38)	I316N	probably damaging	Het
Agrp	G	T	8: 105,566,835 (GRCm38)	T106K	probably damaging	Het
AI429214	T	A	8: 36,994,208 (GRCm38)	V170E	probably benign	Het
Alpk2	G	A	18: 65,350,368 (GRCm38)	Q190*	probably null	Het
Aox3	C	A	1: 58,158,216 (GRCm38)	Q574K	probably benign	Het
Apeh	A	G	9: 108,085,667 (GRCm38)	Y702H	probably damaging	Het
Aqp11	A	G	7: 97,737,485 (GRCm38)	I151T	probably benign	Het
Arhgap28	A	G	17: 67,869,015 (GRCm38)	V363A	possibly damaging	Het
Arhgef18	A	G	8: 3,451,939 (GRCm38)	N699S	probably damaging	Het
Asnsd1	A	G	1: 53,347,317 (GRCm38)	S384P	probably benign	Het
Atl1	G	T	12: 69,931,657 (GRCm38)		probably null	Het
Atp13a2	A	T	4: 140,995,391 (GRCm38)	D203V	possibly damaging	Het
Axdnd1	A	T	1: 156,333,214 (GRCm38)	N164K	probably benign	Het
Bnipl	T	A	3: 95,245,683 (GRCm38)	I162F	probably damaging	Het
Cacna1d	A	T	14: 30,123,163 (GRCm38)	L655I	probably damaging	Het
Canx	T	C	11: 50,304,358 (GRCm38)	I294M	probably damaging	Het
Casz1	T	C	4: 148,946,064 (GRCm38)	F1180S	probably damaging	Het
Cav3	T	C	6: 112,472,383 (GRCm38)	Y121H	probably benign	Het
Cd4	A	C	6: 124,870,536 (GRCm38)	S222A	probably benign	Het
Cds1	T	C	5: 101,812,550 (GRCm38)	I289T	probably benign	Het
Cep112	T	C	11: 108,508,258 (GRCm38)	F328L	probably damaging	Het
Ces4a	G	A	8: 105,138,097 (GRCm38)	G69S	probably damaging	Het
Cfap44	A	T	16: 44,410,475 (GRCm38)	D273V	probably benign	Het
Clec7a	C	T	6: 129,470,955 (GRCm38)	G49D	probably benign	Het
Col22a1	G	A	15: 71,857,253 (GRCm38)	Q599*	probably null	Het
Col6a5	T	A	9: 105,945,600 (GRCm38)	H186L	unknown	Het
Dclk2	C	T	3: 86,805,639 (GRCm38)	R503Q	possibly damaging	Het
Dnah12	A	T	14: 26,724,458 (GRCm38)	R725*	probably null	Het
Dnajc10	G	A	2: 80,350,734 (GRCm38)		probably null	Het
Edem3	A	T	1: 151,794,731 (GRCm38)	H337L	possibly damaging	Het
Eif2ak4	A	T	2: 118,422,123 (GRCm38)	H392L	probably benign	Het
Ercc6l2	G	A	13: 63,848,771 (GRCm38)	V365I	probably damaging	Het
Fam129a	A	G	1: 151,709,133 (GRCm38)	I494V	possibly damaging	Het
Fam129a	A	C	1: 151,696,135 (GRCm38)	E277A	probably damaging	Het
Fan1	T	C	7: 64,346,888 (GRCm38)	E978G	probably damaging	Het
Fcrl6	C	T	1: 172,599,248 (GRCm38)	V44M	probably benign	Het
Gaa	G	A	11: 119,270,282 (GRCm38)	W50*	probably null	Het
Gm10032	T	C	14: 66,792,778 (GRCm38)		noncoding transcript	Het
Gm10985	CTCTAT	CT	3: 53,845,249 (GRCm38)		probably null	Het
Gprc5b	G	T	7: 118,984,175 (GRCm38)	P157Q	probably damaging	Het
Gsdmc3	G	A	15: 63,858,534 (GRCm38)	Q394*	probably null	Het
Gucd1	A	G	10: 75,512,088 (GRCm38)	S38P	probably damaging	Het
Higd1a	A	T	9: 121,850,247 (GRCm38)	I58N	probably damaging	Het
Hmx3	G	C	7: 131,544,549 (GRCm38)	V329L	possibly damaging	Het
Hnrnpul2	A	T	19: 8,824,438 (GRCm38)	R337*	probably null	Het
Idua	T	A	5: 108,681,438 (GRCm38)	H368Q	possibly damaging	Het
Ifih1	A	G	2: 62,623,467 (GRCm38)	V218A	probably benign	Het
Ip6k1	G	A	9: 108,040,996 (GRCm38)	E77K	possibly damaging	Het
Kif1b	G	A	4: 149,187,640 (GRCm38)	S1568L	possibly damaging	Het
Klhl30	T	A	1: 91,358,777 (GRCm38)		probably null	Het
Lasp1	T	A	11: 97,836,134 (GRCm38)	D227E	probably benign	Het
Lhx6	A	G	2: 36,091,324 (GRCm38)	I85T	possibly damaging	Het
Limch1	A	G	5: 67,029,760 (GRCm38)	D840G	probably damaging	Het
Loxl4	C	G	19: 42,603,963 (GRCm38)	E385D	probably damaging	Het
Lrrtm4	A	T	6: 80,021,739 (GRCm38)	I44F	probably damaging	Het
Ltbp2	T	C	12: 84,785,709 (GRCm38)		probably null	Het
Lyg1	T	C	1: 37,950,674 (GRCm38)	Y44C	probably damaging	Het
Maats1	T	C	16: 38,341,762 (GRCm38)	T6A	probably benign	Het
Map1a	A	G	2: 121,298,641 (GRCm38)	I129V	probably damaging	Het
Med27	C	T	2: 29,524,430 (GRCm38)	Q150*	probably null	Het
Ms4a5	A	T	19: 11,279,368 (GRCm38)	I55N	probably damaging	Het
Muc5ac	T	C	7: 141,810,742 (GRCm38)	S2597P	possibly damaging	Het
Mxd1	A	C	6: 86,651,440 (GRCm38)		probably null	Het
Myo3a	A	G	2: 22,578,174 (GRCm38)	D480G	probably benign	Het
Neb	T	C	2: 52,310,638 (GRCm38)	Y343C	probably damaging	Het
Nrn1	A	C	13: 36,730,206 (GRCm38)	V34G	probably damaging	Het
Olfr1090	A	T	2: 86,754,452 (GRCm38)	N95K	probably benign	Het
Olfr1099	T	C	2: 86,959,098 (GRCm38)	Y120C	possibly damaging	Het
Olfr1302	A	G	2: 111,780,496 (GRCm38)	M59V	probably damaging	Het
Olfr679	A	G	7: 105,086,615 (GRCm38)	T300A	probably damaging	Het
Olfr690	C	T	7: 105,329,252 (GRCm38)	W313*	probably null	Het
Olfr722	A	G	14: 49,895,067 (GRCm38)	I245T	probably benign	Het
Olfr887	G	T	9: 38,085,276 (GRCm38)	V147L	probably benign	Het
Pdia4	A	T	6: 47,796,837 (GRCm38)	V526E	probably damaging	Het
Pdia6	T	C	12: 17,278,545 (GRCm38)	V167A	probably damaging	Het
Pgk2	A	G	17: 40,207,509 (GRCm38)	F343L	probably damaging	Het
Piwil1	T	C	5: 128,754,096 (GRCm38)	V827A	probably damaging	Het
Plag1	A	T	4: 3,904,169 (GRCm38)	Y341N	possibly damaging	Het
Plch2	T	C	4: 154,998,999 (GRCm38)	E393G	probably damaging	Het
Ptprg	T	A	14: 12,154,355 (GRCm38)	M692K	probably benign	Het
Rassf8	G	A	6: 145,815,182 (GRCm38)	R78H	probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,110,324 (GRCm38)		probably benign	Het
Rnf213	A	G	11: 119,450,201 (GRCm38)	Q3556R	probably damaging	Het
Rpa2	T	C	4: 132,768,788 (GRCm38)		probably null	Het
Rpe	T	G	1: 66,715,980 (GRCm38)	F174V	possibly damaging	Het
Sbf2	T	C	7: 110,560,295 (GRCm38)	D36G	probably damaging	Het
Scn2a	T	A	2: 65,752,079 (GRCm38)	Y1590*	probably null	Het
Slc24a4	T	C	12: 102,222,759 (GRCm38)	V151A	probably damaging	Het
Slc30a8	A	T	15: 52,295,934 (GRCm38)	M17L	probably benign	Het
Slit3	T	C	11: 35,688,679 (GRCm38)	Y1228H	probably damaging	Het
Smad4	G	A	18: 73,662,744 (GRCm38)	T193M	probably benign	Het
Smtn	T	A	11: 3,530,045 (GRCm38)	H392L	probably benign	Het
St8sia3	A	G	18: 64,269,674 (GRCm38)	D128G	probably damaging	Het
Sucla2	A	T	14: 73,592,668 (GRCm38)	M382L	possibly damaging	Het
Tbx5	A	G	5: 119,836,923 (GRCm38)	T4A	probably benign	Het
Tdrd5	T	C	1: 156,276,573 (GRCm38)	R528G	probably damaging	Het
Tex44	T	C	1: 86,427,089 (GRCm38)	L240P	probably benign	Het
Tns4	A	T	11: 99,080,078 (GRCm38)		probably null	Het
Trappc10	A	T	10: 78,203,924 (GRCm38)	V731E	possibly damaging	Het
Ttf1	A	G	2: 29,071,345 (GRCm38)	K582E	probably damaging	Het
Ttf2	C	A	3: 100,948,193 (GRCm38)	Q895H	possibly damaging	Het
Ttll6	A	G	11: 96,147,532 (GRCm38)	E402G	probably damaging	Het
Ttn	A	T	2: 76,890,092 (GRCm38)		probably null	Het
Ugt8a	T	C	3: 125,875,546 (GRCm38)	D303G	probably damaging	Het
Ulk1	G	T	5: 110,792,436 (GRCm38)	A373D	probably benign	Het
Vmn2r13	A	G	5: 109,158,192 (GRCm38)	S507P	probably benign	Het
Vmn2r19	T	A	6: 123,316,074 (GRCm38)	D358E	possibly damaging	Het
Vmn2r88	T	C	14: 51,413,807 (GRCm38)	S201P	probably benign	Het
Zfp36l2	A	G	17: 84,186,975 (GRCm38)	F78S	probably damaging	Het
Zfp454	A	C	11: 50,873,995 (GRCm38)	S203R	probably benign	Het
Zfp616	C	A	11: 74,085,403 (GRCm38)	Q833K	probably benign	Het
Znhit2	A	G	19: 6,062,061 (GRCm38)	T279A	probably benign	Het
Zswim3	T	A	2: 164,819,993 (GRCm38)	I131N	probably benign	Het

Other mutations in Akap13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Akap13	APN	7	75,725,971 (GRCm38)	missense	probably damaging	0.99
IGL00332:Akap13	APN	7	75,728,919 (GRCm38)	missense	probably damaging	1.00
IGL00481:Akap13	APN	7	75,723,895 (GRCm38)	missense	probably damaging	1.00
IGL00590:Akap13	APN	7	75,610,669 (GRCm38)	missense	probably benign	0.01
IGL00655:Akap13	APN	7	75,704,398 (GRCm38)	missense	probably damaging	0.99
IGL00766:Akap13	APN	7	75,704,512 (GRCm38)	missense	probably damaging	0.96
IGL00818:Akap13	APN	7	75,609,727 (GRCm38)	missense	probably benign	0.00
IGL00826:Akap13	APN	7	75,677,447 (GRCm38)	missense	probably damaging	1.00
IGL01014:Akap13	APN	7	75,750,633 (GRCm38)	utr 3 prime	probably benign
IGL01090:Akap13	APN	7	75,666,531 (GRCm38)	missense	probably benign	0.44
IGL01155:Akap13	APN	7	75,569,936 (GRCm38)	missense	probably damaging	1.00
IGL01326:Akap13	APN	7	75,725,348 (GRCm38)	missense	probably benign	0.30
IGL01456:Akap13	APN	7	75,602,847 (GRCm38)	missense	probably damaging	0.98
IGL01460:Akap13	APN	7	75,747,846 (GRCm38)	missense	probably benign	0.29
IGL01568:Akap13	APN	7	75,608,522 (GRCm38)	nonsense	probably null	0.00
IGL01610:Akap13	APN	7	75,747,605 (GRCm38)	missense	probably damaging	1.00
IGL01610:Akap13	APN	7	75,720,180 (GRCm38)	missense	possibly damaging	0.71
IGL01615:Akap13	APN	7	75,697,393 (GRCm38)	missense	probably damaging	1.00
IGL01667:Akap13	APN	7	75,570,019 (GRCm38)	missense	probably damaging	1.00
IGL01705:Akap13	APN	7	75,746,767 (GRCm38)	missense	possibly damaging	0.86
IGL02070:Akap13	APN	7	75,666,545 (GRCm38)	missense	probably benign	0.27
IGL02269:Akap13	APN	7	75,602,911 (GRCm38)	missense	probably benign
IGL02421:Akap13	APN	7	75,717,806 (GRCm38)	missense	possibly damaging	0.66
IGL02870:Akap13	APN	7	75,609,188 (GRCm38)	missense	probably damaging	0.96
IGL02944:Akap13	APN	7	75,608,657 (GRCm38)	missense	probably benign
IGL03051:Akap13	APN	7	75,610,485 (GRCm38)	nonsense	probably null
IGL03160:Akap13	APN	7	75,730,417 (GRCm38)	missense	probably damaging	1.00
IGL03245:Akap13	APN	7	75,609,752 (GRCm38)	missense	probably damaging	0.99
R0254:Akap13	UTSW	7	75,736,604 (GRCm38)	splice site	probably benign
R0310:Akap13	UTSW	7	75,614,930 (GRCm38)	missense	probably damaging	0.99
R0373:Akap13	UTSW	7	75,730,500 (GRCm38)	missense	probably damaging	1.00
R0373:Akap13	UTSW	7	75,609,929 (GRCm38)	missense	probably benign	0.00
R0408:Akap13	UTSW	7	75,746,796 (GRCm38)	missense	probably damaging	1.00
R0631:Akap13	UTSW	7	75,614,996 (GRCm38)	missense	probably damaging	0.99
R0646:Akap13	UTSW	7	75,747,746 (GRCm38)	missense	probably damaging	1.00
R0781:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	possibly damaging	0.56
R0845:Akap13	UTSW	7	75,725,380 (GRCm38)	missense	probably damaging	1.00
R1004:Akap13	UTSW	7	75,687,286 (GRCm38)	missense	probably damaging	0.99
R1024:Akap13	UTSW	7	75,677,409 (GRCm38)	missense	probably damaging	1.00
R1110:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	possibly damaging	0.56
R1346:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1349:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1372:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1387:Akap13	UTSW	7	75,586,193 (GRCm38)	missense	probably damaging	0.97
R1442:Akap13	UTSW	7	75,735,778 (GRCm38)	missense	probably damaging	0.99
R1466:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1466:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1584:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1696:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1738:Akap13	UTSW	7	75,677,194 (GRCm38)	missense	probably damaging	1.00
R1773:Akap13	UTSW	7	75,683,451 (GRCm38)	missense	possibly damaging	0.80
R1785:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R1786:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R1791:Akap13	UTSW	7	75,611,035 (GRCm38)	missense	probably benign	0.00
R1819:Akap13	UTSW	7	75,608,705 (GRCm38)	missense	probably benign	0.04
R1879:Akap13	UTSW	7	75,610,727 (GRCm38)	missense	probably benign	0.01
R1989:Akap13	UTSW	7	75,704,516 (GRCm38)	missense	probably benign	0.01
R2016:Akap13	UTSW	7	75,704,531 (GRCm38)	missense	probably damaging	0.99
R2092:Akap13	UTSW	7	75,610,570 (GRCm38)	missense	probably benign	0.05
R2131:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2132:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2133:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2251:Akap13	UTSW	7	75,739,477 (GRCm38)	missense	possibly damaging	0.50
R3704:Akap13	UTSW	7	75,666,550 (GRCm38)	missense	probably damaging	1.00
R3713:Akap13	UTSW	7	75,586,181 (GRCm38)	missense	probably damaging	0.98
R3731:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	probably benign	0.39
R3765:Akap13	UTSW	7	75,608,837 (GRCm38)	missense	probably benign	0.04
R3788:Akap13	UTSW	7	75,702,153 (GRCm38)	critical splice donor site	probably null
R3793:Akap13	UTSW	7	75,610,141 (GRCm38)	missense	probably benign	0.00
R3970:Akap13	UTSW	7	75,569,951 (GRCm38)	nonsense	probably null
R4205:Akap13	UTSW	7	75,610,919 (GRCm38)	missense	probably benign	0.05
R4257:Akap13	UTSW	7	75,611,285 (GRCm38)	missense	probably damaging	0.98
R4374:Akap13	UTSW	7	75,608,984 (GRCm38)	missense	probably damaging	0.96
R4448:Akap13	UTSW	7	75,742,760 (GRCm38)	missense	probably damaging	1.00
R4450:Akap13	UTSW	7	75,742,760 (GRCm38)	missense	probably damaging	1.00
R4457:Akap13	UTSW	7	75,739,465 (GRCm38)	missense	probably damaging	0.99
R4458:Akap13	UTSW	7	75,739,465 (GRCm38)	missense	probably damaging	0.99
R4466:Akap13	UTSW	7	75,602,773 (GRCm38)	splice site	probably null
R4632:Akap13	UTSW	7	75,666,553 (GRCm38)	missense	possibly damaging	0.91
R4667:Akap13	UTSW	7	75,729,094 (GRCm38)	missense	probably damaging	1.00
R4669:Akap13	UTSW	7	75,729,094 (GRCm38)	missense	probably damaging	1.00
R4671:Akap13	UTSW	7	75,579,564 (GRCm38)	nonsense	probably null
R4821:Akap13	UTSW	7	75,677,507 (GRCm38)	intron	probably benign
R4868:Akap13	UTSW	7	75,743,504 (GRCm38)	missense	probably damaging	1.00
R4894:Akap13	UTSW	7	75,725,320 (GRCm38)	missense	possibly damaging	0.76
R4943:Akap13	UTSW	7	75,749,240 (GRCm38)	missense	probably benign	0.22
R4962:Akap13	UTSW	7	75,749,430 (GRCm38)	missense	probably damaging	0.98
R4988:Akap13	UTSW	7	75,730,528 (GRCm38)	missense	probably damaging	1.00
R5119:Akap13	UTSW	7	75,687,252 (GRCm38)	missense	probably damaging	0.98
R5141:Akap13	UTSW	7	75,609,614 (GRCm38)	missense	probably benign	0.18
R5419:Akap13	UTSW	7	75,610,243 (GRCm38)	missense	probably benign	0.01
R5427:Akap13	UTSW	7	75,728,869 (GRCm38)	missense	possibly damaging	0.89
R5429:Akap13	UTSW	7	75,602,904 (GRCm38)	missense	possibly damaging	0.70
R5432:Akap13	UTSW	7	75,602,830 (GRCm38)	missense	probably damaging	1.00
R5458:Akap13	UTSW	7	75,586,301 (GRCm38)	missense	probably damaging	1.00
R5636:Akap13	UTSW	7	75,704,372 (GRCm38)	missense	probably damaging	0.96
R5643:Akap13	UTSW	7	75,702,154 (GRCm38)	critical splice donor site	probably null
R5898:Akap13	UTSW	7	75,729,146 (GRCm38)	missense	probably damaging	1.00
R5932:Akap13	UTSW	7	75,610,184 (GRCm38)	missense	probably damaging	1.00
R6135:Akap13	UTSW	7	75,609,908 (GRCm38)	missense	possibly damaging	0.94
R6137:Akap13	UTSW	7	75,677,416 (GRCm38)	missense	probably damaging	1.00
R6182:Akap13	UTSW	7	75,586,280 (GRCm38)	missense	probably benign	0.45
R6310:Akap13	UTSW	7	75,749,193 (GRCm38)	missense	probably damaging	0.99
R6346:Akap13	UTSW	7	75,685,254 (GRCm38)	missense	probably damaging	1.00
R6466:Akap13	UTSW	7	75,727,044 (GRCm38)	missense	probably benign	0.01
R6605:Akap13	UTSW	7	75,579,768 (GRCm38)	missense	probably damaging	0.98
R6617:Akap13	UTSW	7	75,730,363 (GRCm38)	missense	possibly damaging	0.95
R6621:Akap13	UTSW	7	75,569,981 (GRCm38)	missense	probably damaging	1.00
R6703:Akap13	UTSW	7	75,602,898 (GRCm38)	missense	probably damaging	1.00
R6750:Akap13	UTSW	7	75,739,458 (GRCm38)	missense	probably benign	0.03
R7069:Akap13	UTSW	7	75,610,262 (GRCm38)	missense	probably benign	0.29
R7116:Akap13	UTSW	7	75,720,195 (GRCm38)	missense	probably benign	0.00
R7158:Akap13	UTSW	7	75,579,594 (GRCm38)	missense	probably damaging	0.97
R7159:Akap13	UTSW	7	75,730,579 (GRCm38)	missense	possibly damaging	0.72
R7467:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7468:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7471:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7472:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7477:Akap13	UTSW	7	75,749,247 (GRCm38)	missense	probably benign
R7636:Akap13	UTSW	7	75,609,873 (GRCm38)	missense	probably benign	0.04
R7650:Akap13	UTSW	7	75,643,454 (GRCm38)	missense	probably benign	0.20
R7671:Akap13	UTSW	7	75,569,900 (GRCm38)	missense	probably damaging	1.00
R7681:Akap13	UTSW	7	75,728,796 (GRCm38)	missense	possibly damaging	0.91
R7752:Akap13	UTSW	7	75,677,258 (GRCm38)	missense	possibly damaging	0.74
R7784:Akap13	UTSW	7	75,610,328 (GRCm38)	missense	probably benign	0.00
R7816:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7817:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7834:Akap13	UTSW	7	75,742,642 (GRCm38)	missense	possibly damaging	0.85
R7880:Akap13	UTSW	7	75,586,216 (GRCm38)	missense	probably damaging	0.97
R7942:Akap13	UTSW	7	75,611,470 (GRCm38)	missense	possibly damaging	0.50
R8006:Akap13	UTSW	7	75,579,696 (GRCm38)	missense	probably damaging	1.00
R8009:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8011:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8012:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8013:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8016:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8089:Akap13	UTSW	7	75,610,592 (GRCm38)	missense	possibly damaging	0.94
R8138:Akap13	UTSW	7	75,702,231 (GRCm38)	splice site	probably null
R8174:Akap13	UTSW	7	75,728,869 (GRCm38)	missense	possibly damaging	0.89
R8298:Akap13	UTSW	7	75,747,804 (GRCm38)	missense	probably damaging	1.00
R8444:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8445:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8465:Akap13	UTSW	7	75,727,038 (GRCm38)	missense	probably benign	0.11
R8512:Akap13	UTSW	7	75,611,086 (GRCm38)	missense	probably damaging	0.99
R8523:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8793:Akap13	UTSW	7	75,725,328 (GRCm38)	missense	probably benign	0.35
R8907:Akap13	UTSW	7	75,610,708 (GRCm38)	missense	probably damaging	0.99
R8907:Akap13	UTSW	7	75,610,696 (GRCm38)	missense	probably benign	0.08
R8928:Akap13	UTSW	7	75,609,858 (GRCm38)	missense	probably benign	0.00
R8929:Akap13	UTSW	7	75,609,004 (GRCm38)	missense	probably benign	0.00
R8937:Akap13	UTSW	7	75,534,853 (GRCm38)	critical splice donor site	probably null
R8967:Akap13	UTSW	7	75,729,134 (GRCm38)	missense	possibly damaging	0.80
R8986:Akap13	UTSW	7	75,609,326 (GRCm38)	missense	probably benign
R9152:Akap13	UTSW	7	75,611,285 (GRCm38)	missense	probably damaging	0.98
R9153:Akap13	UTSW	7	75,609,481 (GRCm38)	missense	probably benign	0.00
R9160:Akap13	UTSW	7	75,735,778 (GRCm38)	missense	possibly damaging	0.88
R9192:Akap13	UTSW	7	75,704,501 (GRCm38)	missense	probably benign	0.06
R9319:Akap13	UTSW	7	75,609,088 (GRCm38)	missense	probably benign	0.01
R9513:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9515:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9516:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9523:Akap13	UTSW	7	75,643,445 (GRCm38)	missense
R9564:Akap13	UTSW	7	75,609,413 (GRCm38)	missense	probably benign
R9621:Akap13	UTSW	7	75,736,342 (GRCm38)	missense	probably benign	0.09
R9686:Akap13	UTSW	7	75,586,336 (GRCm38)	missense	probably damaging	1.00
Z1176:Akap13	UTSW	7	75,730,552 (GRCm38)	missense	probably damaging	0.99
Z1177:Akap13	UTSW	7	75,615,005 (GRCm38)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- AAAACTTACCTTGCTGTTGACC -3'
(R):5'- GGTAACGTATGCTAGGACATACAC -3'

Sequencing Primer
(F):5'- GTCTCAAAAGTTGGGCTAAGATTG -3'
(R):5'- CGTATGCTAGGACATACACTGAATC -3'

Posted On

2014-09-17