Incidental Mutation 'R2097:Or8k20'
ID 230302
Institutional Source Beutler Lab
Gene Symbol Or8k20
Ensembl Gene ENSMUSG00000075193
Gene Name olfactory receptor family 8 subfamily K member 20
Synonyms Olfr1051, GA_x6K02T2Q125-47756192-47755266, MOR187-5
MMRRC Submission 040101-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.053) question?
Stock # R2097 (G1)
Quality Score 225
Status Validated
Chromosome 2
Chromosomal Location 86105903-86106829 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) A to T at 86106383 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Stop codon at position 149 (Y149*)
Ref Sequence ENSEMBL: ENSMUSP00000150061 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000099898] [ENSMUST00000216432] [ENSMUST00000217245] [ENSMUST00000217294]
AlphaFold Q7TR76
Predicted Effect probably null
Transcript: ENSMUST00000099898
AA Change: Y149*
SMART Domains Protein: ENSMUSP00000097482
Gene: ENSMUSG00000075193
AA Change: Y149*

Pfam:7tm_4 31 308 1.8e-53 PFAM
Pfam:7tm_1 41 290 2.7e-17 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000216432
AA Change: Y149*
Predicted Effect probably null
Transcript: ENSMUST00000217245
AA Change: Y149*
Predicted Effect probably null
Transcript: ENSMUST00000217294
AA Change: Y149*
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.2%
Validation Efficiency 100% (43/43)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actr8 T C 14: 29,709,185 (GRCm39) V263A probably damaging Het
Apol6 T A 15: 76,931,333 (GRCm39) probably null Het
Aqp3 C T 4: 41,098,004 (GRCm39) V36M possibly damaging Het
Bace1 G T 9: 45,771,520 (GRCm39) C478F probably benign Het
Bbof1 C A 12: 84,460,081 (GRCm39) A116D probably damaging Het
Casq1 A T 1: 172,037,988 (GRCm39) L381Q probably damaging Het
Ccdc138 T A 10: 58,397,759 (GRCm39) L533* probably null Het
Cdcp3 A T 7: 130,783,693 (GRCm39) R29* probably null Het
Cnga1 C T 5: 72,776,404 (GRCm39) V20I possibly damaging Het
Cntn6 A G 6: 104,838,910 (GRCm39) E988G probably damaging Het
Cts6 T A 13: 61,343,259 (GRCm39) N321Y probably damaging Het
Dnmt1 A G 9: 20,821,084 (GRCm39) S1269P probably benign Het
Dsg4 C A 18: 20,604,101 (GRCm39) P856H probably damaging Het
Fndc3a C A 14: 72,811,791 (GRCm39) probably null Het
Galc T C 12: 98,218,291 (GRCm39) D187G probably benign Het
Gfm1 T C 3: 67,357,079 (GRCm39) I384T probably damaging Het
Hacd2 A G 16: 34,869,090 (GRCm39) I92V probably benign Het
Hmcn2 T A 2: 31,270,431 (GRCm39) Y1223N probably damaging Het
Il20ra T C 10: 19,635,211 (GRCm39) I484T probably damaging Het
Map7 G T 10: 20,122,362 (GRCm39) V143F probably damaging Het
Mcm3ap T C 10: 76,348,323 (GRCm39) L1893P probably damaging Het
Msh6 A G 17: 88,292,844 (GRCm39) N533S probably benign Het
Nbea T C 3: 55,630,638 (GRCm39) D2233G probably damaging Het
Nlrp6 A T 7: 140,503,117 (GRCm39) T408S probably damaging Het
Notch3 A T 17: 32,341,728 (GRCm39) L2008Q probably damaging Het
Odad4 T A 11: 100,454,408 (GRCm39) F398I possibly damaging Het
Or52e19b T A 7: 103,032,840 (GRCm39) D123V probably damaging Het
Pggt1b T C 18: 46,379,695 (GRCm39) N296D probably benign Het
Pglyrp3 T A 3: 91,935,478 (GRCm39) F243I possibly damaging Het
Phip T C 9: 82,797,392 (GRCm39) H537R possibly damaging Het
Ptch1 T G 13: 63,672,773 (GRCm39) E944A probably benign Het
Ptpdc1 T A 13: 48,746,135 (GRCm39) probably null Het
Ptprq T C 10: 107,489,354 (GRCm39) T924A probably benign Het
Pwp2 C A 10: 78,013,576 (GRCm39) probably benign Het
Slc7a4 G T 16: 17,391,319 (GRCm39) probably null Het
Tmem132b T A 5: 125,715,272 (GRCm39) I327K probably damaging Het
Trim9 T C 12: 70,393,933 (GRCm39) M4V probably damaging Het
Tspan13 T C 12: 36,071,829 (GRCm39) S128G probably benign Het
Zbtb20 A G 16: 43,429,882 (GRCm39) D131G probably null Het
Zeb2 A T 2: 44,887,168 (GRCm39) C615S probably damaging Het
Zfp777 C T 6: 48,021,176 (GRCm39) D149N probably benign Het
Zfp990 A G 4: 145,263,892 (GRCm39) K297E possibly damaging Het
Other mutations in Or8k20
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01578:Or8k20 APN 2 86,106,085 (GRCm39) missense probably damaging 0.99
IGL02263:Or8k20 APN 2 86,106,546 (GRCm39) missense probably benign 0.01
IGL03143:Or8k20 APN 2 86,106,580 (GRCm39) missense probably benign 0.03
R1182:Or8k20 UTSW 2 86,106,612 (GRCm39) missense probably damaging 0.98
R1475:Or8k20 UTSW 2 86,105,905 (GRCm39) makesense probably null
R1712:Or8k20 UTSW 2 86,106,337 (GRCm39) missense probably damaging 0.97
R1903:Or8k20 UTSW 2 86,106,190 (GRCm39) missense probably benign 0.00
R4680:Or8k20 UTSW 2 86,106,517 (GRCm39) missense possibly damaging 0.88
R4879:Or8k20 UTSW 2 86,106,107 (GRCm39) nonsense probably null
R5094:Or8k20 UTSW 2 86,106,384 (GRCm39) missense probably damaging 1.00
R6448:Or8k20 UTSW 2 86,106,691 (GRCm39) missense probably benign 0.07
R8250:Or8k20 UTSW 2 86,106,498 (GRCm39) missense probably damaging 1.00
R9525:Or8k20 UTSW 2 86,106,484 (GRCm39) missense probably benign 0.22
R9761:Or8k20 UTSW 2 86,106,150 (GRCm39) missense probably benign 0.06
X0064:Or8k20 UTSW 2 86,106,462 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2014-09-18