Mutagenetix > Incidental Mutation

Incidental Mutation 'R2101:Vmn2r99'

230534

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

040105-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R2101 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 19377991 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 92 (N92K)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably damaging
Transcript: ENSMUST00000176107
AA Change: N92K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: N92K

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000231989
AA Change: N92K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.6%

Validation Efficiency

100% (67/67)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abtb2	G	A	2: 103,566,862	A46T	probably benign	Het
Agap3	T	A	5: 24,487,799	L47Q	probably damaging	Het
Apaf1	T	G	10: 91,060,080	I377L	probably benign	Het
Astn2	G	A	4: 65,581,686	R937*	probably null	Het
Cacnb1	A	C	11: 98,005,728	V369G	probably damaging	Het
Cdc42bpa	G	T	1: 180,146,968	V1464L	probably benign	Het
Cdkn2aip	A	T	8: 47,713,001	M90K	probably damaging	Het
Chd3	A	T	11: 69,349,051	D1650E	probably benign	Het
Chd9	C	T	8: 91,033,987	P2120L	probably benign	Het
Clca2	T	C	3: 145,077,938	T639A	probably damaging	Het
Cluh	G	T	11: 74,660,502		probably benign	Het
Cnga1	C	T	5: 72,619,061	V20I	possibly damaging	Het
Col11a2	A	T	17: 34,052,169	D528V	probably damaging	Het
Cyfip2	A	G	11: 46,242,443	L810P	probably damaging	Het
D430041D05Rik	A	G	2: 104,148,830	V2084A	probably damaging	Het
Dcc	G	A	18: 71,810,870	H237Y	possibly damaging	Het
Ddx60	T	A	8: 61,940,645	F38L	probably damaging	Het
Dio2	T	C	12: 90,729,823	*130W	probably null	Het
Dock5	G	T	14: 67,794,010	F990L	probably benign	Het
Dpy19l1	A	T	9: 24,482,035	V146E	probably damaging	Het
Dscam	T	C	16: 96,610,349	T1776A	probably benign	Het
Enpep	C	T	3: 129,298,938	S532N	probably benign	Het
Gdf6	A	G	4: 9,860,025	D369G	probably damaging	Het
Gm6169	G	T	13: 97,099,222	R6S	probably damaging	Het
Gtf2b	T	C	3: 142,771,383		probably benign	Het
Hmbox1	A	G	14: 64,828,579		probably benign	Het
Hps3	C	A	3: 20,012,783	V540F	possibly damaging	Het
Hsd3b6	T	A	3: 98,806,237	I249F	possibly damaging	Het
Hunk	T	A	16: 90,432,500		probably null	Het
Kidins220	T	A	12: 25,057,423	L1625Q	probably damaging	Het
Krt1	C	T	15: 101,846,187	G543S	unknown	Het
Myo10	T	A	15: 25,722,259	L186Q	probably benign	Het
N4bp2	T	A	5: 65,790,881	W285R	probably damaging	Het
Nat8l	T	C	5: 33,998,372	L124P	probably damaging	Het
Nemp2	T	C	1: 52,641,066		probably null	Het
Nkain2	G	A	10: 32,329,817	T74I	possibly damaging	Het
Nol4	A	G	18: 22,823,409	S239P	probably damaging	Het
Olfr1223	T	A	2: 89,144,957	E22V	probably benign	Het
Pcbp3	T	C	10: 76,789,755	I152V	possibly damaging	Het
Pcp4l1	G	T	1: 171,175,605	P10Q	probably damaging	Het
Pla1a	T	C	16: 38,415,368	N135D	probably damaging	Het
Plb1	T	A	5: 32,349,660	M1193K	probably damaging	Het
Prc1	T	C	7: 80,312,284	V73A	probably benign	Het
Prkd2	T	A	7: 16,869,565	W807R	probably damaging	Het
Ptprb	C	T	10: 116,315,038		probably benign	Het
Rb1cc1	A	G	1: 6,249,335	I993V	probably benign	Het
Rbp3	C	T	14: 33,956,018	T641M	probably damaging	Het
Rev3l	T	C	10: 39,828,096	V2046A	probably benign	Het
Rnpep	G	T	1: 135,271,617	N334K	probably damaging	Het
Sarm1	G	A	11: 78,475,289	P695S	probably damaging	Het
Sf3a3	C	T	4: 124,718,343	T131I	possibly damaging	Het
Slc38a10	A	T	11: 120,132,741	V283E	probably damaging	Het
Slco6c1	A	T	1: 97,072,870	L552*	probably null	Het
Tas2r136	T	C	6: 132,777,532	M211V	probably benign	Het
Tax1bp3	G	A	11: 73,181,121	D65N	probably damaging	Het
Tdrd5	A	G	1: 156,301,639	F167S	probably damaging	Het
Tfeb	G	A	17: 47,789,665	V269M	probably damaging	Het
Tspan12	A	T	6: 21,799,888	F153L	probably benign	Het
Txnrd1	T	G	10: 82,881,739	L186V	probably damaging	Het
Upk1b	A	T	16: 38,780,137	C160*	probably null	Het
Uty	T	C	Y: 1,176,541	Q172R	probably damaging	Het
Vmn1r23	T	C	6: 57,926,452	K114E	possibly damaging	Het
Vstm2a	T	A	11: 16,263,191	M192K	probably benign	Het
Zc3h12c	A	G	9: 52,116,421	V547A	probably benign	Het
Zfp944	T	A	17: 22,339,828	N146I	probably benign	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19378854	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19394256	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19382623	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19393658	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19380115	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19380232	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19378690	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19378223	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19394343	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19394573	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19379043	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19362259	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19380060	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19362252	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19377945	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19362153	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19378815	missense	probably benign	0.01
R2474:Vmn2r99	UTSW	17	19378629	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19378708	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19394373	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19378570	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19379260	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19393662	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19362135	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19378606	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19379339	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19379269	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19394146	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19377948	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19378980	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19382605	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19380031	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19380034	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19380195	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19378110	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19394564	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19393710	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19379311	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19379145	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19393817	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19380040	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19393758	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19394181	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19393660	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19378126	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19378627	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19362301	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19379301	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- CTAGTTCAACTGTTCATGGTGAAG -3'
(R):5'- CCCAGTTAGAGCAGATGTTATCC -3'

Sequencing Primer
(F):5'- CTCTACTAAATATGTAGATGTGTATG -3'
(R):5'- TCCAAGAAGTTCCTGTCAGTGCAG -3'

Posted On

2014-09-18