Mutagenetix > Incidental Mutation

Incidental Mutation 'R2117:Prdm16'

231047

Institutional Source

Beutler Lab

Gene Symbol

Prdm16

Ensembl Gene

ENSMUSG00000039410

Gene Name

PR domain containing 16

Synonyms

line 27, Mel1, 5730557K01Rik, csp1

MMRRC Submission

040121-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.731) question?

Stock #

R2117 (G1)

Quality Score

212

Status

Not validated

Chromosome

Chromosomal Location

154400582-154721330 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 154432382 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Cysteine at position 296 (S296C)

Ref Sequence

ENSEMBL: ENSMUSP00000101263 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030902] [ENSMUST00000070313] [ENSMUST00000097759] [ENSMUST00000105636] [ENSMUST00000105637] [ENSMUST00000105638] [ENSMUST00000181143]

AlphaFold

A2A935

Predicted Effect

probably null
Transcript: ENSMUST00000030902
AA Change: S296C

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000030902
Gene: ENSMUSG00000039410
AA Change: S296C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	217	8.75e-5	SMART
ZnF_C2H2	230	250	2.82e1	SMART
low complexity region	260	274	N/A	INTRINSIC
ZnF_C2H2	282	304	1.04e-3	SMART
ZnF_C2H2	310	332	1.6e-4	SMART
ZnF_C2H2	338	361	3.95e-4	SMART
ZnF_C2H2	367	389	1.95e-3	SMART
ZnF_C2H2	395	417	8.22e-2	SMART
ZnF_C2H2	424	451	9.96e0	SMART
low complexity region	496	517	N/A	INTRINSIC
low complexity region	520	540	N/A	INTRINSIC
low complexity region	610	641	N/A	INTRINSIC
ZnF_C2H2	951	973	5.29e-5	SMART
ZnF_C2H2	979	1002	1.6e-4	SMART
ZnF_C2H2	1008	1030	7.26e-3	SMART
low complexity region	1116	1132	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000070313
AA Change: S297C

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064546
Gene: ENSMUSG00000039410
AA Change: S297C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	218	8.75e-5	SMART
ZnF_C2H2	231	251	2.82e1	SMART
low complexity region	261	275	N/A	INTRINSIC
ZnF_C2H2	283	305	1.04e-3	SMART
ZnF_C2H2	311	333	1.6e-4	SMART
ZnF_C2H2	339	362	3.95e-4	SMART
ZnF_C2H2	368	390	1.95e-3	SMART
ZnF_C2H2	396	418	8.22e-2	SMART
ZnF_C2H2	425	452	9.96e0	SMART
low complexity region	497	518	N/A	INTRINSIC
low complexity region	521	541	N/A	INTRINSIC
low complexity region	611	642	N/A	INTRINSIC
ZnF_C2H2	953	975	5.29e-5	SMART
ZnF_C2H2	981	1004	1.6e-4	SMART
ZnF_C2H2	1010	1032	7.26e-3	SMART
low complexity region	1118	1134	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000097759
AA Change: S296C

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000095365
Gene: ENSMUSG00000039410
AA Change: S296C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	217	8.75e-5	SMART
ZnF_C2H2	230	250	2.82e1	SMART
low complexity region	260	274	N/A	INTRINSIC
ZnF_C2H2	282	304	1.04e-3	SMART
ZnF_C2H2	310	332	1.6e-4	SMART
ZnF_C2H2	338	361	3.95e-4	SMART
ZnF_C2H2	367	389	1.95e-3	SMART
ZnF_C2H2	395	417	8.22e-2	SMART
ZnF_C2H2	424	451	9.96e0	SMART
low complexity region	496	517	N/A	INTRINSIC
low complexity region	520	540	N/A	INTRINSIC
low complexity region	610	641	N/A	INTRINSIC
ZnF_C2H2	952	974	5.29e-5	SMART
ZnF_C2H2	980	1003	1.6e-4	SMART
ZnF_C2H2	1009	1031	7.26e-3	SMART
low complexity region	1117	1133	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105636
AA Change: S297C

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000101261
Gene: ENSMUSG00000039410
AA Change: S297C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	218	8.75e-5	SMART
ZnF_C2H2	231	251	2.82e1	SMART
low complexity region	261	275	N/A	INTRINSIC
ZnF_C2H2	283	305	1.04e-3	SMART
ZnF_C2H2	311	333	1.6e-4	SMART
ZnF_C2H2	339	362	3.95e-4	SMART
ZnF_C2H2	368	390	1.95e-3	SMART
ZnF_C2H2	396	418	8.22e-2	SMART
ZnF_C2H2	425	452	9.96e0	SMART
low complexity region	497	518	N/A	INTRINSIC
low complexity region	521	541	N/A	INTRINSIC
low complexity region	611	642	N/A	INTRINSIC
ZnF_C2H2	952	974	5.29e-5	SMART
ZnF_C2H2	980	1003	1.6e-4	SMART
ZnF_C2H2	1009	1031	7.26e-3	SMART
low complexity region	1117	1133	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105637
AA Change: S295C

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000101262
Gene: ENSMUSG00000039410
AA Change: S295C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	216	8.75e-5	SMART
ZnF_C2H2	229	249	2.82e1	SMART
low complexity region	259	273	N/A	INTRINSIC
ZnF_C2H2	281	303	1.04e-3	SMART
ZnF_C2H2	309	331	1.6e-4	SMART
ZnF_C2H2	337	360	3.95e-4	SMART
ZnF_C2H2	366	388	1.95e-3	SMART
ZnF_C2H2	394	416	8.22e-2	SMART
ZnF_C2H2	423	450	9.96e0	SMART
low complexity region	495	516	N/A	INTRINSIC
low complexity region	519	539	N/A	INTRINSIC
low complexity region	609	640	N/A	INTRINSIC
ZnF_C2H2	950	972	5.29e-5	SMART
ZnF_C2H2	978	1001	1.6e-4	SMART
ZnF_C2H2	1007	1029	7.26e-3	SMART
low complexity region	1115	1131	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105638
AA Change: S296C

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000101263
Gene: ENSMUSG00000039410
AA Change: S296C

Domain	Start	End	E-Value	Type
low complexity region	44	57	N/A	INTRINSIC
low complexity region	74	83	N/A	INTRINSIC
SET	84	217	8.75e-5	SMART
ZnF_C2H2	230	250	2.82e1	SMART
low complexity region	260	274	N/A	INTRINSIC
ZnF_C2H2	282	304	1.04e-3	SMART
ZnF_C2H2	310	332	1.6e-4	SMART
ZnF_C2H2	338	361	3.95e-4	SMART
ZnF_C2H2	367	389	1.95e-3	SMART
ZnF_C2H2	395	417	8.22e-2	SMART
ZnF_C2H2	424	451	9.96e0	SMART
low complexity region	496	517	N/A	INTRINSIC
low complexity region	520	540	N/A	INTRINSIC
low complexity region	610	641	N/A	INTRINSIC
ZnF_C2H2	952	974	5.29e-5	SMART
ZnF_C2H2	980	1003	1.6e-4	SMART
ZnF_C2H2	1009	1031	7.26e-3	SMART
low complexity region	1117	1133	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131762

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141164

Predicted Effect

probably benign
Transcript: ENSMUST00000181143

SMART Domains

Protein: ENSMUSP00000138001
Gene: ENSMUSG00000097871

Domain	Start	End	E-Value	Type
low complexity region	17	30	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mutant mice exhibit a cleft secondary palate, whitening of brown adipose tissue in older or fed a high-fat diet, and impaired adaptive thermogenesis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 101 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	A	T	13: 81,640,656 (GRCm39)	C3357S	probably benign	Het
Ago1	T	A	4: 126,357,650 (GRCm39)		probably null	Het
Akap10	A	T	11: 61,781,129 (GRCm39)	D562E	possibly damaging	Het
Akr1b7	G	A	6: 34,395,929 (GRCm39)	A144T	possibly damaging	Het
Ankle1	A	G	8: 71,860,562 (GRCm39)	T340A	probably benign	Het
Arf5	C	T	6: 28,424,783 (GRCm39)	Q71*	probably null	Het
Arl15	C	T	13: 114,104,196 (GRCm39)	S111F	probably damaging	Het
Asph	G	A	4: 9,517,671 (GRCm39)	Q468*	probably null	Het
Bcam	G	T	7: 19,492,352 (GRCm39)	A581E	possibly damaging	Het
Blvra	G	T	2: 126,927,989 (GRCm39)	E80*	probably null	Het
Ccr6	T	C	17: 8,474,914 (GRCm39)	F40L	possibly damaging	Het
Cfap161	A	T	7: 83,425,184 (GRCm39)	N302K	possibly damaging	Het
Ckmt2	T	A	13: 92,003,964 (GRCm39)	I345F	probably benign	Het
Cpsf6	A	G	10: 117,202,025 (GRCm39)		probably benign	Het
Ctnna1	A	G	18: 35,285,678 (GRCm39)	N8S	possibly damaging	Het
Cyp2d11	A	G	15: 82,275,954 (GRCm39)	L209P	probably damaging	Het
Dab2	T	C	15: 6,465,096 (GRCm39)	V628A	probably damaging	Het
Dcstamp	C	T	15: 39,618,571 (GRCm39)	Q327*	probably null	Het
Defb38	A	T	8: 19,073,483 (GRCm39)	Y63*	probably null	Het
Dlg5	A	T	14: 24,227,826 (GRCm39)	L365*	probably null	Het
Dnai7	C	T	6: 145,150,967 (GRCm39)		probably null	Het
Dnmt3l	C	A	10: 77,899,130 (GRCm39)	L110I	probably damaging	Het
Exoc3l2	G	T	7: 19,228,907 (GRCm39)	L108F	possibly damaging	Het
Exoc4	T	A	6: 33,324,760 (GRCm39)	N351K	possibly damaging	Het
Fam83h	C	A	15: 75,876,582 (GRCm39)	E252*	probably null	Het
Fancm	A	G	12: 65,123,948 (GRCm39)	D202G	probably damaging	Het
Fat1	T	A	8: 45,490,500 (GRCm39)	V3804E	probably benign	Het
Fbxw28	T	C	9: 109,159,985 (GRCm39)	T190A	probably benign	Het
Fer1l4	T	C	2: 155,881,038 (GRCm39)	T843A	probably benign	Het
Fnip1	A	T	11: 54,391,450 (GRCm39)	H461L	probably damaging	Het
Gcn1	A	T	5: 115,736,884 (GRCm39)	M1276L	probably benign	Het
Gemin4	A	G	11: 76,101,827 (GRCm39)	V978A	possibly damaging	Het
Gm7247	A	T	14: 51,602,792 (GRCm39)	I43F	probably damaging	Het
Gm9978	C	T	10: 78,322,731 (GRCm39)		noncoding transcript	Het
Gpr4	T	C	7: 18,957,070 (GRCm39)	S331P	probably damaging	Het
Hspa1a	T	A	17: 35,189,455 (GRCm39)	N483Y	probably damaging	Het
Ift74	A	G	4: 94,515,496 (GRCm39)	T138A	probably benign	Het
Ints14	T	G	9: 64,887,077 (GRCm39)	L336R	probably damaging	Het
Irak1	G	T	X: 73,066,218 (GRCm39)	P197Q	possibly damaging	Het
Kif4	A	G	X: 99,709,323 (GRCm39)	S315G	probably benign	Het
L3mbtl1	T	A	2: 162,801,990 (GRCm39)		probably null	Het
Lamb3	A	G	1: 193,016,489 (GRCm39)	R657G	probably benign	Het
Ldc1	C	A	4: 130,109,156 (GRCm39)	V295L	possibly damaging	Het
Lrp2	A	C	2: 69,313,729 (GRCm39)	V2334G	probably benign	Het
Lrwd1	T	A	5: 136,159,332 (GRCm39)	Y431F	probably damaging	Het
Map3k21	A	T	8: 126,650,781 (GRCm39)	H261L	probably benign	Het
Meis1	G	T	11: 18,831,679 (GRCm39)	P453Q	probably damaging	Het
Mettl16	G	T	11: 74,693,755 (GRCm39)	M255I	probably benign	Het
Mllt10	A	G	2: 18,167,380 (GRCm39)	N435S	probably benign	Het
Mta2	T	C	19: 8,920,880 (GRCm39)	I27T	probably damaging	Het
Nav2	A	G	7: 49,114,328 (GRCm39)	I771V	probably benign	Het
Nisch	A	T	14: 30,899,242 (GRCm39)		probably benign	Het
Npc1	G	A	18: 12,329,613 (GRCm39)	P990L	probably damaging	Het
Nrxn1	A	T	17: 91,011,705 (GRCm39)	I308K	probably damaging	Het
Or13l2	C	A	3: 97,317,765 (GRCm39)	C244F	probably damaging	Het
Or1ad6	A	T	11: 50,860,147 (GRCm39)	I101F	probably damaging	Het
Or5h17	T	C	16: 58,820,783 (GRCm39)	L245P	possibly damaging	Het
Otogl	T	C	10: 107,694,779 (GRCm39)	D823G	probably benign	Het
P2rx7	A	G	5: 122,819,329 (GRCm39)	T584A	probably benign	Het
Pals1	T	C	12: 78,856,696 (GRCm39)	F180L	possibly damaging	Het
Pank1	T	A	19: 34,818,486 (GRCm39)	I18F	probably damaging	Het
Pgap3	A	G	11: 98,281,933 (GRCm39)	L126P	probably damaging	Het
Pgap6	C	T	17: 26,336,858 (GRCm39)	L259F	possibly damaging	Het
Phka1	C	T	X: 101,653,807 (GRCm39)	R290H	probably damaging	Het
Pkd2	G	A	5: 104,631,042 (GRCm39)	E489K	probably damaging	Het
Prex2	T	A	1: 11,256,937 (GRCm39)	N1216K	probably damaging	Het
Prmt7	A	G	8: 106,953,930 (GRCm39)	T124A	probably damaging	Het
Ptpra	G	T	2: 130,381,655 (GRCm39)	R372L	probably damaging	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Rap2b	G	T	3: 61,272,512 (GRCm39)	G12V	probably damaging	Het
Rapgef5	T	A	12: 117,677,799 (GRCm39)		probably null	Het
Rassf4	A	G	6: 116,622,088 (GRCm39)	F168S	possibly damaging	Het
Rtf1	T	A	2: 119,535,999 (GRCm39)	H184Q	probably benign	Het
Sacs	A	G	14: 61,451,220 (GRCm39)	K4422R	probably benign	Het
Sec11c	A	T	18: 65,933,720 (GRCm39)	T9S	probably benign	Het
Sema3d	C	T	5: 12,613,240 (GRCm39)	T439I	probably benign	Het
Sephs1	A	G	2: 4,904,351 (GRCm39)	N243S	probably benign	Het
Setd2	TTGGGA	T	9: 110,433,212 (GRCm39)		probably null	Het
Setx	A	G	2: 29,020,313 (GRCm39)	D100G	probably benign	Het
Slc22a7	C	A	17: 46,744,898 (GRCm39)	V383L	possibly damaging	Het
Slc25a40	T	C	5: 8,480,417 (GRCm39)	C56R	probably damaging	Het
Stk38l	T	A	6: 146,670,344 (GRCm39)	L229I	probably damaging	Het
Sult2a5	T	C	7: 13,359,359 (GRCm39)	S112P	probably damaging	Het
Syt4	A	G	18: 31,573,520 (GRCm39)	Y332H	probably damaging	Het
Tcof1	T	C	18: 60,965,857 (GRCm39)	E415G	possibly damaging	Het
Tenm2	A	G	11: 35,915,681 (GRCm39)	L1951P	probably damaging	Het
Tlr9	A	G	9: 106,102,536 (GRCm39)	N609S	probably damaging	Het
Tmem120a	A	T	5: 135,764,977 (GRCm39)	S266T	possibly damaging	Het
Tmem132b	A	G	5: 125,699,615 (GRCm39)	E92G	probably damaging	Het
Tnfrsf18	T	A	4: 156,112,973 (GRCm39)	V196E	probably damaging	Het
Trpc1	A	T	9: 95,599,637 (GRCm39)	L474H	probably damaging	Het
Trpm6	T	C	19: 18,807,316 (GRCm39)	V1020A	probably damaging	Het
Usp20	T	A	2: 30,906,317 (GRCm39)	C562S	probably damaging	Het
Usp47	T	A	7: 111,666,443 (GRCm39)		probably null	Het
Vgll1	A	C	X: 56,137,790 (GRCm39)	K53T	probably damaging	Het
Vmn1r26	T	C	6: 57,985,335 (GRCm39)	N285D	possibly damaging	Het
Zfp445	T	A	9: 122,682,502 (GRCm39)	K480*	probably null	Het
Zfp786	A	G	6: 47,803,931 (GRCm39)	V37A	probably damaging	Het
Zfp821	A	G	8: 110,447,851 (GRCm39)	E64G	probably damaging	Het
Zfp994	T	A	17: 22,419,962 (GRCm39)	D329V	probably damaging	Het
Zkscan8	T	C	13: 21,704,488 (GRCm39)	S484G	probably damaging	Het

Other mutations in Prdm16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00987:Prdm16	APN	4	154,426,426 (GRCm39)	missense	possibly damaging	0.89
IGL01517:Prdm16	APN	4	154,412,882 (GRCm39)	missense	probably damaging	1.00
IGL01810:Prdm16	APN	4	154,432,384 (GRCm39)	missense	probably benign	0.10
IGL02260:Prdm16	APN	4	154,412,744 (GRCm39)	missense	probably benign	0.00
IGL02623:Prdm16	APN	4	154,425,334 (GRCm39)	missense	probably damaging	0.98
IGL02744:Prdm16	APN	4	154,429,910 (GRCm39)	missense	probably damaging	1.00
R0126:Prdm16	UTSW	4	154,413,295 (GRCm39)	splice site	probably benign
R0345:Prdm16	UTSW	4	154,425,568 (GRCm39)	missense	probably benign	0.09
R0365:Prdm16	UTSW	4	154,426,513 (GRCm39)	missense	probably damaging	1.00
R0440:Prdm16	UTSW	4	154,561,084 (GRCm39)	splice site	probably benign
R0899:Prdm16	UTSW	4	154,613,366 (GRCm39)	missense	probably damaging	1.00
R1127:Prdm16	UTSW	4	154,613,256 (GRCm39)	missense	probably damaging	1.00
R1546:Prdm16	UTSW	4	154,613,117 (GRCm39)	missense	possibly damaging	0.94
R1803:Prdm16	UTSW	4	154,419,718 (GRCm39)	missense	probably damaging	1.00
R3814:Prdm16	UTSW	4	154,412,750 (GRCm39)	missense	probably damaging	1.00
R4348:Prdm16	UTSW	4	154,561,124 (GRCm39)	missense	probably benign	0.00
R4458:Prdm16	UTSW	4	154,406,765 (GRCm39)	missense	probably benign
R4557:Prdm16	UTSW	4	154,613,284 (GRCm39)	missense	probably benign	0.01
R4581:Prdm16	UTSW	4	154,407,810 (GRCm39)	missense	probably damaging	1.00
R4584:Prdm16	UTSW	4	154,422,140 (GRCm39)	missense	probably damaging	1.00
R4627:Prdm16	UTSW	4	154,451,697 (GRCm39)	missense	probably damaging	1.00
R4926:Prdm16	UTSW	4	154,426,009 (GRCm39)	missense	possibly damaging	0.74
R5055:Prdm16	UTSW	4	154,419,719 (GRCm39)	missense	possibly damaging	0.48
R5152:Prdm16	UTSW	4	154,430,559 (GRCm39)	missense	probably damaging	0.99
R5257:Prdm16	UTSW	4	154,451,671 (GRCm39)	missense	possibly damaging	0.89
R5258:Prdm16	UTSW	4	154,451,671 (GRCm39)	missense	possibly damaging	0.89
R5368:Prdm16	UTSW	4	154,429,848 (GRCm39)	missense	probably damaging	1.00
R5464:Prdm16	UTSW	4	154,430,601 (GRCm39)	critical splice acceptor site	probably null
R5640:Prdm16	UTSW	4	154,426,367 (GRCm39)	missense	probably benign
R5744:Prdm16	UTSW	4	154,613,161 (GRCm39)	missense	probably damaging	1.00
R5892:Prdm16	UTSW	4	154,407,716 (GRCm39)	missense	possibly damaging	0.74
R5938:Prdm16	UTSW	4	154,432,411 (GRCm39)	missense	probably damaging	0.99
R6380:Prdm16	UTSW	4	154,425,824 (GRCm39)	missense	probably benign	0.00
R6784:Prdm16	UTSW	4	154,407,764 (GRCm39)	missense	probably damaging	1.00
R7097:Prdm16	UTSW	4	154,429,925 (GRCm39)	missense	probably damaging	1.00
R7181:Prdm16	UTSW	4	154,613,094 (GRCm39)	missense	probably damaging	1.00
R7197:Prdm16	UTSW	4	154,425,967 (GRCm39)	missense	probably damaging	1.00
R7273:Prdm16	UTSW	4	154,429,910 (GRCm39)	missense	probably damaging	1.00
R7379:Prdm16	UTSW	4	154,613,316 (GRCm39)	missense	probably damaging	1.00
R7641:Prdm16	UTSW	4	154,429,901 (GRCm39)	missense	probably damaging	1.00
R7704:Prdm16	UTSW	4	154,425,947 (GRCm39)	missense	probably damaging	1.00
R7751:Prdm16	UTSW	4	154,412,756 (GRCm39)	missense	probably damaging	1.00
R8048:Prdm16	UTSW	4	154,405,339 (GRCm39)	missense	probably damaging	1.00
R8225:Prdm16	UTSW	4	154,439,702 (GRCm39)	critical splice donor site	probably null
R8503:Prdm16	UTSW	4	154,426,009 (GRCm39)	missense	probably benign	0.04
R8683:Prdm16	UTSW	4	154,613,161 (GRCm39)	missense	probably damaging	1.00
R8880:Prdm16	UTSW	4	154,613,370 (GRCm39)	missense	probably damaging	1.00
R9257:Prdm16	UTSW	4	154,422,155 (GRCm39)	missense	probably damaging	1.00
R9259:Prdm16	UTSW	4	154,430,525 (GRCm39)	missense	possibly damaging	0.69
RF008:Prdm16	UTSW	4	154,426,452 (GRCm39)	missense	probably damaging	1.00
X0010:Prdm16	UTSW	4	154,407,834 (GRCm39)	missense	probably damaging	1.00
Z1176:Prdm16	UTSW	4	154,426,243 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- AGCGGACCAGGACCTTTATG -3'
(R):5'- ACATTCCGCTGTGATGAGTG -3'

Sequencing Primer
(F):5'- CACATGGTTCCCAGTTCA -3'
(R):5'- TGATGAGCTCTTCCAGTGCAG -3'

Posted On

2014-09-18