Mutagenetix > Incidental Mutation

Incidental Mutation 'R2087:Rapgef6'

231529

Institutional Source

Beutler Lab

Gene Symbol

Rapgef6

Ensembl Gene

ENSMUSG00000037533

Gene Name

Rap guanine nucleotide exchange factor (GEF) 6

Synonyms

PDZ-GEF2, Pdzgef2, C030018K18Rik, RA-GEF-2

MMRRC Submission

040092-MU

Accession Numbers

Genbank: NM_175258; MGI: 2384761

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2087 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

54522847-54699285 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 54631249 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 291 (T291A)

Ref Sequence

ENSEMBL: ENSMUSP00000151509 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094536] [ENSMUST00000101206] [ENSMUST00000102743] [ENSMUST00000108894] [ENSMUST00000108895] [ENSMUST00000207429] [ENSMUST00000218995]

AlphaFold

Q5NCJ1

Predicted Effect

probably damaging
Transcript: ENSMUST00000094536
AA Change: T153A

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000092114
Gene: ENSMUSG00000037533
AA Change: T153A

Domain	Start	End	E-Value	Type
cNMP	1	113	6.64e-7	SMART
RasGEFN	127	240	4.35e-33	SMART
PDZ	255	327	8.86e-16	SMART
low complexity region	409	420	N/A	INTRINSIC
RA	464	550	1.47e-20	SMART
RasGEF	571	853	3.88e-84	SMART
low complexity region	944	957	N/A	INTRINSIC
low complexity region	972	989	N/A	INTRINSIC
low complexity region	1016	1061	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101206
AA Change: T438A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098766
Gene: ENSMUSG00000037533
AA Change: T438A

Domain	Start	End	E-Value	Type
internal_repeat_1	10	82	1.45e-5	PROSPERO
low complexity region	187	205	N/A	INTRINSIC
low complexity region	231	239	N/A	INTRINSIC
cNMP	280	398	4.8e-13	SMART
RasGEFN	412	525	4.35e-33	SMART
PDZ	540	612	8.86e-16	SMART
low complexity region	694	705	N/A	INTRINSIC
RA	749	835	1.47e-20	SMART
RasGEF	856	1095	5.35e-87	SMART
low complexity region	1237	1250	N/A	INTRINSIC
low complexity region	1270	1293	N/A	INTRINSIC
low complexity region	1345	1364	N/A	INTRINSIC
low complexity region	1368	1380	N/A	INTRINSIC
low complexity region	1444	1452	N/A	INTRINSIC
low complexity region	1555	1568	N/A	INTRINSIC
low complexity region	1591	1604	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000102743
AA Change: T438A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000099804
Gene: ENSMUSG00000037533
AA Change: T438A

Domain	Start	End	E-Value	Type
internal_repeat_1	10	82	1.42e-5	PROSPERO
low complexity region	187	205	N/A	INTRINSIC
low complexity region	231	239	N/A	INTRINSIC
cNMP	280	398	4.8e-13	SMART
RasGEFN	412	525	4.35e-33	SMART
PDZ	540	612	8.86e-16	SMART
low complexity region	694	705	N/A	INTRINSIC
RA	749	835	1.47e-20	SMART
RasGEF	856	1138	3.88e-84	SMART
low complexity region	1229	1242	N/A	INTRINSIC
low complexity region	1262	1285	N/A	INTRINSIC
low complexity region	1337	1356	N/A	INTRINSIC
low complexity region	1360	1372	N/A	INTRINSIC
low complexity region	1436	1444	N/A	INTRINSIC
low complexity region	1547	1560	N/A	INTRINSIC
low complexity region	1583	1596	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108894
AA Change: T153A

PolyPhen 2 Score 0.859 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000104522
Gene: ENSMUSG00000037533
AA Change: T153A

Domain	Start	End	E-Value	Type
cNMP	1	113	6.64e-7	SMART
RasGEFN	127	240	4.35e-33	SMART
PDZ	255	327	8.86e-16	SMART
low complexity region	409	420	N/A	INTRINSIC
RA	464	550	1.47e-20	SMART
RasGEF	571	810	5.35e-87	SMART
low complexity region	952	965	N/A	INTRINSIC
low complexity region	980	997	N/A	INTRINSIC
low complexity region	1024	1069	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000108895
AA Change: T438A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000104523
Gene: ENSMUSG00000037533
AA Change: T438A

Domain	Start	End	E-Value	Type
internal_repeat_1	10	82	1.95e-5	PROSPERO
low complexity region	187	205	N/A	INTRINSIC
low complexity region	231	239	N/A	INTRINSIC
cNMP	280	398	4.8e-13	SMART
RasGEFN	412	526	1.03e-25	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149372

Predicted Effect

probably damaging
Transcript: ENSMUST00000207429
AA Change: T438A

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000218995
AA Change: T291A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220269

Meta Mutation Damage Score

0.1760

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

95% (57/60)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit an inlarged spleen, increased IgE and IgG levels and altered cytokine production. [provided by MGI curators]

Allele List at MGI

All alleles(16) : Targeted, knock-out(1) Targeted, other(2) Gene trapped(13)

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700021P04Rik	T	A	19: 24,065,000 (GRCm38)		noncoding transcript	Het
Adamts8	G	A	9: 30,962,112 (GRCm38)	R871Q	probably damaging	Het
Ank1	T	C	8: 23,093,811 (GRCm38)	L310S	probably damaging	Het
Atp2a3	A	G	11: 72,980,448 (GRCm38)	T592A	probably damaging	Het
Bpifb6	A	G	2: 153,906,078 (GRCm38)	D219G	possibly damaging	Het
Catsper4	T	C	4: 134,226,605 (GRCm38)	N81S	probably damaging	Het
Ccdc14	T	C	16: 34,695,645 (GRCm38)		probably null	Het
Ces1c	A	C	8: 93,107,602 (GRCm38)	N353K	probably benign	Het
Chrna6	T	A	8: 27,407,127 (GRCm38)	M241L	probably benign	Het
Chtf8	T	A	8: 106,885,936 (GRCm38)	R124*	probably null	Het
Cpt1b	A	G	15: 89,422,208 (GRCm38)	V296A	probably benign	Het
Cryl1	G	A	14: 57,275,945 (GRCm38)	S273L	possibly damaging	Het
Ctps	T	C	4: 120,562,815 (GRCm38)	D134G	probably benign	Het
Cyp4f18	T	C	8: 72,000,988 (GRCm38)	M138V	probably benign	Het
Dnajb12	A	G	10: 59,890,845 (GRCm38)	K107R	possibly damaging	Het
Epha1	C	T	6: 42,363,568 (GRCm38)	D590N	probably benign	Het
Fndc3b	A	G	3: 27,451,554 (GRCm38)	V855A	probably benign	Het
Ganc	A	G	2: 120,457,257 (GRCm38)	Y822C	probably damaging	Het
Hook2	A	G	8: 85,002,691 (GRCm38)	D622G	probably damaging	Het
Kcnj8	T	A	6: 142,565,696 (GRCm38)	N395I	probably benign	Het
Kdm4b	T	C	17: 56,389,564 (GRCm38)	S427P	possibly damaging	Het
Krt12	A	G	11: 99,418,633 (GRCm38)	F267S	probably damaging	Het
Lrrn2	T	G	1: 132,937,751 (GRCm38)	F185V	probably damaging	Het
Mecom	A	G	3: 29,952,814 (GRCm38)	S764P	probably benign	Het
Mfsd14b	C	G	13: 65,067,982 (GRCm38)	G386R	probably damaging	Het
Mrm2	A	G	5: 140,328,400 (GRCm38)	S227P	probably damaging	Het
Mug1	A	G	6: 121,856,291 (GRCm38)	N285S	probably benign	Het
Nalcn	T	C	14: 123,281,145 (GRCm38)	T1661A	probably benign	Het
Ncoa6	G	A	2: 155,406,159 (GRCm38)	R1742*	probably null	Het
Nln	T	A	13: 104,037,369 (GRCm38)	H548L	probably damaging	Het
Nrros	C	T	16: 32,144,157 (GRCm38)	W311*	probably null	Het
Olfr457	A	T	6: 42,472,051 (GRCm38)	N42K	probably damaging	Het
Olfr688	T	A	7: 105,288,186 (GRCm38)	I31N	probably benign	Het
Pgm5	T	C	19: 24,733,563 (GRCm38)	Y425C	probably damaging	Het
Pld2	A	G	11: 70,542,960 (GRCm38)	D242G	probably damaging	Het
Plekhm1	A	G	11: 103,397,025 (GRCm38)		probably null	Het
Ptdss1	T	C	13: 66,976,817 (GRCm38)		probably benign	Het
Pyroxd2	G	T	19: 42,733,770 (GRCm38)	L415I	probably benign	Het
Rb1	A	G	14: 73,280,252 (GRCm38)	I238T	probably benign	Het
Rp1	G	A	1: 4,348,352 (GRCm38)	H846Y	probably damaging	Het
Rpgrip1	T	A	14: 52,136,622 (GRCm38)		probably null	Het
Scaf4	T	C	16: 90,252,425 (GRCm38)	D258G	unknown	Het
Ska1	A	T	18: 74,206,849 (GRCm38)	C9S	probably benign	Het
Skp2	C	A	15: 9,113,698 (GRCm38)	G376C	probably damaging	Het
Slc43a1	G	A	2: 84,849,831 (GRCm38)	R107Q	probably damaging	Het
Slc44a3	T	A	3: 121,525,670 (GRCm38)	N141Y	probably damaging	Het
Slit1	A	T	19: 41,637,483 (GRCm38)	D613E	probably benign	Het
Spata22	A	T	11: 73,340,253 (GRCm38)	Y111F	probably benign	Het
Tchh	C	A	3: 93,443,918 (GRCm38)	R222S	unknown	Het
Tekt3	A	G	11: 63,094,697 (GRCm38)	D443G	possibly damaging	Het
Tns2	A	T	15: 102,107,119 (GRCm38)	Q144L	possibly damaging	Het
Troap	T	C	15: 99,078,817 (GRCm38)	V274A	possibly damaging	Het
Ugt1a8	A	G	1: 88,088,273 (GRCm38)	Y136C	probably damaging	Het
Vmn2r23	A	T	6: 123,741,499 (GRCm38)	M604L	probably benign	Het
Vps13b	T	A	15: 35,597,493 (GRCm38)	W1060R	probably damaging	Het
Wdfy3	A	G	5: 101,895,060 (GRCm38)	S1942P	probably damaging	Het
Wdr90	G	A	17: 25,846,603 (GRCm38)	T1596I	probably damaging	Het
Zc3h4	A	G	7: 16,416,940 (GRCm38)	E69G	possibly damaging	Het

Other mutations in Rapgef6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00436:Rapgef6	APN	11	54,679,265 (GRCm38)	missense	probably benign	0.00
IGL00507:Rapgef6	APN	11	54,664,109 (GRCm38)	nonsense	probably null
IGL00809:Rapgef6	APN	11	54,649,300 (GRCm38)	missense	probably damaging	1.00
IGL00843:Rapgef6	APN	11	54,691,273 (GRCm38)	missense	probably benign	0.03
IGL00899:Rapgef6	APN	11	54,620,018 (GRCm38)	nonsense	probably null
IGL01372:Rapgef6	APN	11	54,668,611 (GRCm38)	splice site	probably benign
IGL01604:Rapgef6	APN	11	54,694,563 (GRCm38)	missense	probably damaging	0.99
IGL01935:Rapgef6	APN	11	54,610,842 (GRCm38)	missense	possibly damaging	0.78
IGL01991:Rapgef6	APN	11	54,552,869 (GRCm38)	missense	probably benign	0.37
IGL02243:Rapgef6	APN	11	54,676,400 (GRCm38)	missense	probably damaging	1.00
IGL02407:Rapgef6	APN	11	54,676,355 (GRCm38)	missense	possibly damaging	0.91
IGL02676:Rapgef6	APN	11	54,649,346 (GRCm38)	unclassified	probably benign
IGL02934:Rapgef6	APN	11	54,625,864 (GRCm38)	missense	probably damaging	1.00
IGL03076:Rapgef6	APN	11	54,625,967 (GRCm38)	missense	probably damaging	1.00
IGL03110:Rapgef6	APN	11	54,696,089 (GRCm38)	missense	probably damaging	0.97
IGL03256:Rapgef6	APN	11	54,657,429 (GRCm38)	missense	probably damaging	1.00
shocker	UTSW	11	54,620,016 (GRCm38)	missense	probably damaging	1.00
D4216:Rapgef6	UTSW	11	54,668,746 (GRCm38)	splice site	probably benign
PIT4305001:Rapgef6	UTSW	11	54,679,377 (GRCm38)	missense	probably damaging	1.00
PIT4366001:Rapgef6	UTSW	11	54,691,620 (GRCm38)	missense	probably damaging	0.98
R0047:Rapgef6	UTSW	11	54,546,378 (GRCm38)	missense	possibly damaging	0.65
R0047:Rapgef6	UTSW	11	54,546,378 (GRCm38)	missense	possibly damaging	0.65
R0125:Rapgef6	UTSW	11	54,625,875 (GRCm38)	nonsense	probably null
R0189:Rapgef6	UTSW	11	54,691,249 (GRCm38)	missense	probably benign
R0201:Rapgef6	UTSW	11	54,619,941 (GRCm38)	missense	probably damaging	1.00
R0505:Rapgef6	UTSW	11	54,625,963 (GRCm38)	missense	probably benign	0.00
R0524:Rapgef6	UTSW	11	54,690,284 (GRCm38)	missense	probably benign	0.32
R0853:Rapgef6	UTSW	11	54,668,677 (GRCm38)	missense	probably damaging	1.00
R1203:Rapgef6	UTSW	11	54,691,699 (GRCm38)	missense	probably benign	0.09
R1440:Rapgef6	UTSW	11	54,626,708 (GRCm38)	missense	probably damaging	1.00
R1453:Rapgef6	UTSW	11	54,639,727 (GRCm38)	splice site	probably null
R1530:Rapgef6	UTSW	11	54,661,183 (GRCm38)	missense	probably damaging	1.00
R1593:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1620:Rapgef6	UTSW	11	54,626,594 (GRCm38)	missense	possibly damaging	0.88
R1628:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1629:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1630:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1634:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1640:Rapgef6	UTSW	11	54,657,405 (GRCm38)	missense	probably damaging	1.00
R1686:Rapgef6	UTSW	11	54,691,632 (GRCm38)	missense	possibly damaging	0.81
R1722:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1743:Rapgef6	UTSW	11	54,676,284 (GRCm38)	missense	probably damaging	1.00
R1816:Rapgef6	UTSW	11	54,694,488 (GRCm38)	missense	probably benign
R1851:Rapgef6	UTSW	11	54,642,811 (GRCm38)	missense	probably benign	0.01
R1852:Rapgef6	UTSW	11	54,642,811 (GRCm38)	missense	probably benign	0.01
R1868:Rapgef6	UTSW	11	54,546,397 (GRCm38)	frame shift	probably null
R1888:Rapgef6	UTSW	11	54,660,828 (GRCm38)	missense	probably damaging	1.00
R1888:Rapgef6	UTSW	11	54,660,828 (GRCm38)	missense	probably damaging	1.00
R1942:Rapgef6	UTSW	11	54,657,263 (GRCm38)	missense	possibly damaging	0.95
R1943:Rapgef6	UTSW	11	54,657,263 (GRCm38)	missense	possibly damaging	0.95
R2031:Rapgef6	UTSW	11	54,552,858 (GRCm38)	missense	probably benign	0.30
R2106:Rapgef6	UTSW	11	54,668,686 (GRCm38)	missense	probably benign	0.17
R2362:Rapgef6	UTSW	11	54,694,272 (GRCm38)	missense	probably damaging	1.00
R2484:Rapgef6	UTSW	11	54,642,756 (GRCm38)	missense	possibly damaging	0.48
R2566:Rapgef6	UTSW	11	54,687,711 (GRCm38)	missense	possibly damaging	0.66
R2872:Rapgef6	UTSW	11	54,661,175 (GRCm38)	missense	probably damaging	1.00
R2872:Rapgef6	UTSW	11	54,661,175 (GRCm38)	missense	probably damaging	1.00
R3744:Rapgef6	UTSW	11	54,625,934 (GRCm38)	missense	probably benign	0.40
R3848:Rapgef6	UTSW	11	54,691,308 (GRCm38)	missense	probably damaging	0.97
R4823:Rapgef6	UTSW	11	54,694,500 (GRCm38)	missense	probably benign	0.08
R4859:Rapgef6	UTSW	11	54,636,163 (GRCm38)	missense	probably benign
R4906:Rapgef6	UTSW	11	54,552,836 (GRCm38)	missense	probably damaging	1.00
R4911:Rapgef6	UTSW	11	54,622,317 (GRCm38)	missense	probably damaging	0.97
R4937:Rapgef6	UTSW	11	54,657,317 (GRCm38)	missense	probably damaging	1.00
R5033:Rapgef6	UTSW	11	54,691,381 (GRCm38)	missense	possibly damaging	0.92
R5249:Rapgef6	UTSW	11	54,523,117 (GRCm38)	missense	probably benign	0.19
R5304:Rapgef6	UTSW	11	54,657,374 (GRCm38)	missense	probably benign	0.01
R5656:Rapgef6	UTSW	11	54,636,136 (GRCm38)	missense	possibly damaging	0.95
R5701:Rapgef6	UTSW	11	54,676,394 (GRCm38)	missense	possibly damaging	0.76
R5758:Rapgef6	UTSW	11	54,668,644 (GRCm38)	missense	probably damaging	1.00
R5973:Rapgef6	UTSW	11	54,639,783 (GRCm38)	missense	probably damaging	1.00
R6177:Rapgef6	UTSW	11	54,620,016 (GRCm38)	missense	probably damaging	1.00
R6268:Rapgef6	UTSW	11	54,649,247 (GRCm38)	missense	probably damaging	1.00
R6287:Rapgef6	UTSW	11	54,626,338 (GRCm38)	splice site	probably null
R6293:Rapgef6	UTSW	11	54,634,781 (GRCm38)	missense	probably damaging	1.00
R6471:Rapgef6	UTSW	11	54,691,737 (GRCm38)	missense	probably damaging	0.99
R6863:Rapgef6	UTSW	11	54,546,380 (GRCm38)	missense	probably benign	0.00
R6950:Rapgef6	UTSW	11	54,676,380 (GRCm38)	missense	probably benign	0.09
R7144:Rapgef6	UTSW	11	54,657,365 (GRCm38)	missense	possibly damaging	0.78
R7171:Rapgef6	UTSW	11	54,676,363 (GRCm38)	missense	possibly damaging	0.94
R7199:Rapgef6	UTSW	11	54,546,426 (GRCm38)	missense	probably benign	0.00
R7291:Rapgef6	UTSW	11	54,691,239 (GRCm38)	missense	probably benign	0.05
R7436:Rapgef6	UTSW	11	54,610,921 (GRCm38)	critical splice donor site	probably null
R7498:Rapgef6	UTSW	11	54,620,004 (GRCm38)	missense	probably damaging	1.00
R7506:Rapgef6	UTSW	11	54,636,171 (GRCm38)	missense	probably benign	0.00
R7527:Rapgef6	UTSW	11	54,634,961 (GRCm38)	missense	unknown
R7646:Rapgef6	UTSW	11	54,625,954 (GRCm38)	missense	probably benign	0.00
R7655:Rapgef6	UTSW	11	54,694,453 (GRCm38)	missense	probably benign	0.10
R7656:Rapgef6	UTSW	11	54,694,453 (GRCm38)	missense	probably benign	0.10
R7687:Rapgef6	UTSW	11	54,661,075 (GRCm38)	missense	possibly damaging	0.93
R7768:Rapgef6	UTSW	11	54,626,588 (GRCm38)	missense	probably damaging	1.00
R7788:Rapgef6	UTSW	11	54,694,399 (GRCm38)	missense	probably damaging	1.00
R7890:Rapgef6	UTSW	11	54,626,723 (GRCm38)	missense	probably damaging	1.00
R8113:Rapgef6	UTSW	11	54,625,958 (GRCm38)	missense	probably benign	0.03
R8337:Rapgef6	UTSW	11	54,631,301 (GRCm38)	nonsense	probably null
R8393:Rapgef6	UTSW	11	54,687,661 (GRCm38)	missense	probably benign
R8465:Rapgef6	UTSW	11	54,691,482 (GRCm38)	missense	probably benign	0.00
R8492:Rapgef6	UTSW	11	54,690,237 (GRCm38)	missense	probably damaging	0.99
R8791:Rapgef6	UTSW	11	54,568,469 (GRCm38)	missense	probably benign	0.15
R8866:Rapgef6	UTSW	11	54,552,874 (GRCm38)	critical splice donor site	probably null
R8917:Rapgef6	UTSW	11	54,691,566 (GRCm38)	nonsense	probably null
R8921:Rapgef6	UTSW	11	54,679,239 (GRCm38)	missense	probably benign	0.09
R9031:Rapgef6	UTSW	11	54,687,841 (GRCm38)	missense	probably benign	0.00
R9093:Rapgef6	UTSW	11	54,597,086 (GRCm38)	nonsense	probably null
R9354:Rapgef6	UTSW	11	54,619,923 (GRCm38)	missense	possibly damaging	0.66
R9514:Rapgef6	UTSW	11	54,552,858 (GRCm38)	missense	probably benign	0.14
R9516:Rapgef6	UTSW	11	54,691,343 (GRCm38)	missense	probably damaging	1.00
R9739:Rapgef6	UTSW	11	54,622,363 (GRCm38)	missense	probably benign	0.03
R9789:Rapgef6	UTSW	11	54,649,271 (GRCm38)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TCTAAGCCTCTGGTCGCTAC -3'
(R):5'- AGGCTCTTTCGTTAGTAAAATGACC -3'

Sequencing Primer
(F):5'- AAGCCTCTGGTCGCTACTAAATTAC -3'
(R):5'- CTTTCGTTAGTAAAATGACCAAACAC -3'

Posted On

2014-09-18