Mutagenetix > Incidental Mutation

Incidental Mutation 'R2093:Cyp2g1'

231910

Institutional Source

Beutler Lab

Gene Symbol

Cyp2g1

Ensembl Gene

ENSMUSG00000049685

Gene Name

cytochrome P450, family 2, subfamily g, polypeptide 1

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2093 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

26808892-26821205 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 26819433 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 418 (D418G)

Ref Sequence

ENSEMBL: ENSMUSP00000047150 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040944]

AlphaFold

Q9WV19

Predicted Effect

probably benign
Transcript: ENSMUST00000040944
AA Change: D418G

PolyPhen 2 Score 0.353 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000047150
Gene: ENSMUSG00000049685
AA Change: D418G

Domain	Start	End	E-Value	Type
low complexity region	12	23	N/A	INTRINSIC
Pfam:p450	34	491	4e-150	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205273

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for disruptions in this gene display an essentially normal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931408C20Rik	G	T	1: 26,682,141	Y1319*	probably null	Het
AA986860	A	G	1: 130,743,304	D421G	probably benign	Het
Adamts1	T	G	16: 85,802,445	Q89P	probably benign	Het
Arhgef10l	T	G	4: 140,570,290	N277T	possibly damaging	Het
Atp2c1	G	A	9: 105,418,121	R669*	probably null	Het
Brip1	T	C	11: 86,139,145	T558A	possibly damaging	Het
Cbr2	G	A	11: 120,730,429	T148I	probably benign	Het
Ccdc110	C	A	8: 45,942,077	T335K	probably damaging	Het
Cd44	T	G	2: 102,814,284	D731A	probably damaging	Het
Cnot1	T	C	8: 95,775,358	D44G	probably damaging	Het
Cnrip1	T	C	11: 17,052,237	V23A	probably damaging	Het
Cntnap5c	A	T	17: 58,199,000	H673L	probably benign	Het
Col3a1	C	T	1: 45,332,990	A493V	probably damaging	Het
Crem	A	T	18: 3,299,256	V19E	probably damaging	Het
Dmrta1	A	T	4: 89,691,505	H234L	probably benign	Het
Drc3	C	T	11: 60,370,484	R154W	probably damaging	Het
Eef1d	A	G	15: 75,902,701	S370P	probably benign	Het
Fdx1l	T	C	9: 21,073,424	H28R	probably benign	Het
Fmo5	A	G	3: 97,645,878	I381V	probably benign	Het
Fpr-rs4	CAGGAA	CA	17: 18,022,334		probably null	Het
Fras1	T	C	5: 96,781,203	L3822P	probably damaging	Het
Gm13212	C	A	4: 145,622,569	T192N	probably benign	Het
Gm3604	G	T	13: 62,369,606	H313N	possibly damaging	Het
Heatr4	T	C	12: 83,975,081	E460G	possibly damaging	Het
Ino80	T	A	2: 119,426,670	H834L	possibly damaging	Het
Insr	A	T	8: 3,204,762	C331S	probably damaging	Het
Itih4	C	T	14: 30,891,737	L304F	probably damaging	Het
Klhdc3	C	T	17: 46,677,953	V104I	probably benign	Het
Lrp2	C	T	2: 69,536,021	D245N	probably benign	Het
Map1b	T	C	13: 99,429,670	E2181G	unknown	Het
Map2	T	C	1: 66,399,440	V41A	probably damaging	Het
Mical3	A	T	6: 121,040,386	H156Q	probably damaging	Het
Mrgpra2b	C	T	7: 47,464,160	V249I	probably benign	Het
Myo5b	T	A	18: 74,759,192	L1669Q	probably damaging	Het
Nme8	G	A	13: 19,650,872	S548F	probably damaging	Het
Npas1	A	T	7: 16,459,277	N408K	probably benign	Het
Olfr1076	A	C	2: 86,509,243	Q261H	probably damaging	Het
Olfr339	A	T	2: 36,421,929	Y177F	probably benign	Het
Pcnx4	T	C	12: 72,579,442	Y1141H	probably damaging	Het
Pmaip1	C	A	18: 66,460,981	P64Q	probably damaging	Het
Prrxl1	C	T	14: 32,647,155		probably benign	Het
Ranbp3	T	A	17: 56,710,145	M387K	probably damaging	Het
Rbm4	A	G	19: 4,787,764	Y231H	probably damaging	Het
Rpgrip1l	T	C	8: 91,270,132	S105G	possibly damaging	Het
Rsf1	CG	CGACGGCGGGG	7: 97,579,908		probably benign	Het
Rtn3	A	T	19: 7,456,850	D573E	probably damaging	Het
Rxrg	T	C	1: 167,627,324	C159R	probably damaging	Het
Sdk2	T	C	11: 113,943,122	Y78C	probably damaging	Het
Smu1	A	G	4: 40,738,438	V432A	probably benign	Het
Spag1	T	C	15: 36,224,130	L609P	probably damaging	Het
Spata21	T	G	4: 141,096,966	V180G	probably benign	Het
Sptlc3	A	T	2: 139,625,874	I451F	possibly damaging	Het
Srl	A	G	16: 4,523,032	C8R	unknown	Het
Tmprss13	A	G	9: 45,345,042	R485G	probably damaging	Het
Trpv4	C	T	5: 114,635,504	A266T	probably damaging	Het
Vmn1r198	A	G	13: 22,354,685	T25A	probably benign	Het
Vmn2r77	T	C	7: 86,801,494	V196A	probably benign	Het
Vmn2r82	C	T	10: 79,395,979	T604I	probably benign	Het
Zfp354a	C	T	11: 51,069,724	T254I	probably damaging	Het

Other mutations in Cyp2g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01134:Cyp2g1	APN	7	26809831	missense	probably benign	0.05
IGL01137:Cyp2g1	APN	7	26814259	missense	possibly damaging	0.87
IGL02052:Cyp2g1	APN	7	26814294	splice site	probably benign
IGL02338:Cyp2g1	APN	7	26814804	splice site	probably benign
IGL02452:Cyp2g1	APN	7	26811446	missense	probably benign	0.28
IGL02523:Cyp2g1	APN	7	26819187	missense	probably damaging	1.00
IGL03165:Cyp2g1	APN	7	26809776	missense	possibly damaging	0.94
IGL03230:Cyp2g1	APN	7	26819403	missense	probably damaging	1.00
PIT4472001:Cyp2g1	UTSW	7	26814194	missense	probably benign	0.28
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0380:Cyp2g1	UTSW	7	26814295	splice site	probably benign
R0697:Cyp2g1	UTSW	7	26814727	nonsense	probably null
R0830:Cyp2g1	UTSW	7	26814791	missense	probably benign	0.00
R1660:Cyp2g1	UTSW	7	26809682	critical splice acceptor site	probably null
R2131:Cyp2g1	UTSW	7	26820710	missense	probably damaging	0.99
R4606:Cyp2g1	UTSW	7	26814154	missense	possibly damaging	0.80
R5030:Cyp2g1	UTSW	7	26820801	missense	probably benign	0.06
R5574:Cyp2g1	UTSW	7	26820740	missense	possibly damaging	0.81
R5877:Cyp2g1	UTSW	7	26816640	missense	possibly damaging	0.80
R6745:Cyp2g1	UTSW	7	26814179	missense	probably damaging	1.00
R7040:Cyp2g1	UTSW	7	26820759	missense	probably damaging	0.99
R7223:Cyp2g1	UTSW	7	26814632	missense	probably damaging	0.98
R7934:Cyp2g1	UTSW	7	26819193	missense	probably damaging	1.00
R8112:Cyp2g1	UTSW	7	26819461	missense	probably benign
R8177:Cyp2g1	UTSW	7	26819153	missense	probably damaging	1.00
R8194:Cyp2g1	UTSW	7	26814734	missense	possibly damaging	0.89
R9043:Cyp2g1	UTSW	7	26809831	missense	probably benign	0.05
R9406:Cyp2g1	UTSW	7	26819485	critical splice donor site	probably null
R9441:Cyp2g1	UTSW	7	26814635	missense	possibly damaging	0.72
X0067:Cyp2g1	UTSW	7	26820762	missense	possibly damaging	0.70

Predicted Primers

PCR Primer
(F):5'- AAGATTGTACCCCAGTGTTGTCC -3'
(R):5'- CCTATGGCTAGGTTTACTAAATGTTTG -3'

Sequencing Primer
(F):5'- GTACCCCAGTGTTGTCCTGTCAC -3'
(R):5'- AGTCAGGATCTCACGTTAGCC -3'

Posted On

2014-09-18