Mutagenetix > Incidental Mutation

Incidental Mutation 'R2095:Vmn2r8'

232035

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r8

Ensembl Gene

ENSMUSG00000090961

Gene Name

vomeronasal 2, receptor 8

Synonyms

EG627479

MMRRC Submission

040099-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R2095 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108797193-108808754 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 108808621 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 45 (D45A)

Ref Sequence

ENSEMBL: ENSMUSP00000126953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172140]

AlphaFold

L7N472

Predicted Effect

possibly damaging
Transcript: ENSMUST00000172140
AA Change: D45A

PolyPhen 2 Score 0.938 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000126953
Gene: ENSMUSG00000090961
AA Change: D45A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	419	1.1e-28	PFAM
Pfam:NCD3G	507	561	8.2e-18	PFAM
Pfam:7tm_3	594	829	1.1e-54	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 95.6%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530002B09Rik	C	A	4: 122,702,358	Y127*	probably null	Het
Adam33	C	T	2: 131,053,709	G562D	probably damaging	Het
Adh1	T	C	3: 138,282,796	F177L	probably damaging	Het
Arhgef17	T	C	7: 100,881,263	T1439A	probably damaging	Het
Arl5c	C	T	11: 97,993,451	E105K	probably damaging	Het
Armc3	T	A	2: 19,288,929	D510E	possibly damaging	Het
Bbx	T	C	16: 50,224,689	E395G	possibly damaging	Het
Cacna2d2	G	A	9: 107,527,165	E1087K	probably benign	Het
Casq1	T	C	1: 172,215,962	N113S	probably benign	Het
Cbfa2t3	A	G	8: 122,634,988	S432P	probably benign	Het
Cd163	A	G	6: 124,317,822	D615G	probably damaging	Het
Chrnb2	A	T	3: 89,761,437	D190E	probably benign	Het
Clec9a	T	C	6: 129,416,358	L115P	possibly damaging	Het
Cluh	C	T	11: 74,661,724	R532*	probably null	Het
Cntnap5a	T	A	1: 116,442,260	L869Q	probably damaging	Het
Ctgf	T	C	10: 24,596,479	V140A	probably benign	Het
Cyp3a57	A	T	5: 145,369,134	K143*	probably null	Het
Dcaf12	G	T	4: 41,294,085	H351N	probably benign	Het
Dtna	T	A	18: 23,569,748	L112Q	probably damaging	Het
Fnta	G	A	8: 25,999,879	Q303*	probably null	Het
Frs2	T	C	10: 117,074,602	E285G	probably benign	Het
Gabra4	T	C	5: 71,624,112	I293V	probably damaging	Het
Gm2431	A	T	7: 142,257,781	C129S	unknown	Het
Gnl2	C	T	4: 125,034,318	R49C	probably damaging	Het
Helz	T	A	11: 107,646,146	Y275N	probably damaging	Het
Hydin	A	G	8: 110,462,657	E1231G	probably damaging	Het
Kctd11	T	A	11: 69,879,576	D212V	probably damaging	Het
Klf3	T	A	5: 64,821,902	M29K	probably benign	Het
Klhl21	T	C	4: 152,009,393	S151P	probably benign	Het
Lsm14b	A	T	2: 180,031,787		probably benign	Het
Mlxipl	T	C	5: 135,122,120		probably benign	Het
Mov10	G	A	3: 104,801,531	R389C	probably damaging	Het
Msh6	A	G	17: 87,988,233	M1071V	possibly damaging	Het
Myh8	G	T	11: 67,286,224	A401S	probably benign	Het
Ndufv3	T	C	17: 31,527,486	S117P	possibly damaging	Het
Obscn	A	T	11: 59,093,584		probably null	Het
Olfr1205	T	C	2: 88,831,290	Y58H	probably damaging	Het
Olfr150	T	A	9: 39,737,261	Y149N	probably damaging	Het
Olfr166	T	C	16: 19,486,931	I31T	probably benign	Het
Olfr381	T	A	11: 73,486,594	T77S	probably damaging	Het
Pcdhb17	T	A	18: 37,486,322	D388E	probably benign	Het
Pgm3	T	C	9: 86,556,341	T423A	probably damaging	Het
Rufy4	T	C	1: 74,147,663	C537R	probably damaging	Het
S1pr5	T	A	9: 21,244,858	T91S	probably benign	Het
Sgcz	T	C	8: 37,540,392		probably benign	Het
Slc33a1	A	G	3: 63,963,955	L79P	probably damaging	Het
Slco2a1	A	G	9: 103,076,968	T383A	probably benign	Het
Slit1	G	A	19: 41,606,380	R1184W	probably damaging	Het
Spta1	T	A	1: 174,244,198	M2248K	possibly damaging	Het
Stub1	A	G	17: 25,830,890	Y304H	probably damaging	Het
Taar7e	T	A	10: 24,038,051	Y146*	probably null	Het
Tbc1d1	C	T	5: 64,316,501	S660L	probably benign	Het
Tcea2	C	A	2: 181,686,932	F259L	probably damaging	Het
Tcp11l1	T	A	2: 104,681,840	K482N	probably damaging	Het
Trpv6	C	T	6: 41,621,756	R645Q	probably damaging	Het
Uaca	T	A	9: 60,840,843	S30T	probably benign	Het
Ubap2	C	G	4: 41,206,901	V492L	possibly damaging	Het
Ube3c	T	C	5: 29,668,040	F1026S	probably damaging	Het
Zfp738	G	T	13: 67,671,303	L180I	probably damaging	Het

Other mutations in Vmn2r8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02957:Vmn2r8	APN	5	108802225	missense	probably benign	0.01
R0324:Vmn2r8	UTSW	5	108797941	splice site	probably null
R0335:Vmn2r8	UTSW	5	108797451	splice site	probably null
R0394:Vmn2r8	UTSW	5	108802072	missense	probably benign	0.12
R0501:Vmn2r8	UTSW	5	108803183	missense	probably benign	0.03
R0615:Vmn2r8	UTSW	5	108799329	missense	probably damaging	1.00
R0678:Vmn2r8	UTSW	5	108800546	missense	probably benign	0.00
R1167:Vmn2r8	UTSW	5	108803176	missense	probably benign	0.01
R1187:Vmn2r8	UTSW	5	108803219	nonsense	probably null
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1406:Vmn2r8	UTSW	5	108802368	missense	probably benign
R1451:Vmn2r8	UTSW	5	108798067	missense	probably damaging	1.00
R1535:Vmn2r8	UTSW	5	108802174	missense	probably damaging	1.00
R1795:Vmn2r8	UTSW	5	108803106	missense	probably benign
R1874:Vmn2r8	UTSW	5	108802418	missense	possibly damaging	0.74
R1908:Vmn2r8	UTSW	5	108797570	missense	probably benign	0.03
R1925:Vmn2r8	UTSW	5	108802153	missense	probably damaging	0.97
R1960:Vmn2r8	UTSW	5	108799286	missense	probably damaging	0.99
R1961:Vmn2r8	UTSW	5	108798095	missense	probably benign	0.45
R1967:Vmn2r8	UTSW	5	108802383	missense	probably benign	0.01
R2159:Vmn2r8	UTSW	5	108802303	missense	probably benign	0.22
R4240:Vmn2r8	UTSW	5	108797503	missense	probably damaging	0.99
R4581:Vmn2r8	UTSW	5	108801704	missense	probably benign	0.03
R4744:Vmn2r8	UTSW	5	108808581	missense	probably benign	0.00
R4755:Vmn2r8	UTSW	5	108801700	missense	probably benign	0.03
R4917:Vmn2r8	UTSW	5	108797398	missense	probably damaging	1.00
R4957:Vmn2r8	UTSW	5	108799263	missense	probably benign	0.16
R5141:Vmn2r8	UTSW	5	108808706	missense	probably damaging	0.96
R5481:Vmn2r8	UTSW	5	108801770	missense	probably benign	0.09
R5571:Vmn2r8	UTSW	5	108802240	missense	probably damaging	1.00
R5624:Vmn2r8	UTSW	5	108802459	missense	probably damaging	0.99
R6003:Vmn2r8	UTSW	5	108797382	missense	probably damaging	1.00
R6243:Vmn2r8	UTSW	5	108799345	missense	probably benign	0.01
R6265:Vmn2r8	UTSW	5	108808597	missense	probably benign
R6315:Vmn2r8	UTSW	5	108801891	missense	probably benign
R6413:Vmn2r8	UTSW	5	108801723	missense	probably benign	0.09
R7120:Vmn2r8	UTSW	5	108808638	missense	possibly damaging	0.56
R7406:Vmn2r8	UTSW	5	108800576	missense	probably benign	0.00
R7409:Vmn2r8	UTSW	5	108808583	nonsense	probably null
R7489:Vmn2r8	UTSW	5	108797656	missense	possibly damaging	0.95
R7532:Vmn2r8	UTSW	5	108802240	missense	probably benign	0.22
R7534:Vmn2r8	UTSW	5	108802174	missense	possibly damaging	0.94
R7739:Vmn2r8	UTSW	5	108802177	missense	probably damaging	1.00
R8099:Vmn2r8	UTSW	5	108801834	missense	probably benign
R8245:Vmn2r8	UTSW	5	108798070	missense	probably damaging	1.00
R8711:Vmn2r8	UTSW	5	108798096	missense	possibly damaging	0.89
R8781:Vmn2r8	UTSW	5	108797731	missense	possibly damaging	0.95
R8874:Vmn2r8	UTSW	5	108808751	missense	probably damaging	1.00
R8927:Vmn2r8	UTSW	5	108802265	missense
R8928:Vmn2r8	UTSW	5	108802265	missense
R9288:Vmn2r8	UTSW	5	108802319	missense	probably benign	0.39
R9596:Vmn2r8	UTSW	5	108799330	missense	possibly damaging	0.94
R9652:Vmn2r8	UTSW	5	108803241	missense	probably benign	0.18
Z1088:Vmn2r8	UTSW	5	108801998	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCTGGCATTGTGAACTGGAATC -3'
(R):5'- TGCAGAAGAAACGGATTTCAC -3'

Sequencing Primer
(F):5'- GCATTGTGAACTGGAATCCATTG -3'
(R):5'- GGATTTCACAACTACTCCATCTTAG -3'

Posted On

2014-09-18