|Institutional Source||Beutler Lab|
|Gene Name||CKLF-like MARVEL transmembrane domain containing 8|
|Is this an essential gene?||Probably non essential (E-score: 0.090)|
|Stock #||R2107 (G1)|
|Chromosomal Location||114789345-114844156 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 114796108 bp|
|Amino Acid Change||Valine to Aspartic acid at position 85 (V85D)|
|Ref Sequence||ENSEMBL: ENSMUSP00000043706 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000047013]|
|Predicted Effect||possibly damaging
AA Change: V85D
PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
AA Change: V85D
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.5749|
|Coding Region Coverage||
|Validation Efficiency||100% (72/72)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 3. This gene acts as a tumor suppressor, and plays a role in regulating the migration of tumor cells. The encoded protein is thought to function as a a negative regulator of epidermal growth factor-induced signaling. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cmtm8||
(F):5'- TGTGCTGGCTTCACTCTCAG -3'
(R):5'- GGCATTGTGACTAATGACTTCCTC -3'
(F):5'- ACTCTCAGGCCATCGGGTAAG -3'
(R):5'- TGTGACTAATGACTTCCTCTGTATC -3'