Mutagenetix > Incidental Mutation

Incidental Mutation 'R2110:Olfr965'

232466

Institutional Source

Beutler Lab

Gene Symbol

Olfr965

Ensembl Gene

ENSMUSG00000095839

Gene Name

olfactory receptor 965

Synonyms

GA_x6K02T2PVTD-33416730-33417668, MOR171-28

MMRRC Submission

040114-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R2110 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

39711353-39720614 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 39719722 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 165 (R165Q)

Ref Sequence

ENSEMBL: ENSMUSP00000150401 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069342] [ENSMUST00000213335] [ENSMUST00000215164]

AlphaFold

Q7TRA7

Predicted Effect

probably benign
Transcript: ENSMUST00000069342
AA Change: R165Q

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000069696
Gene: ENSMUSG00000095839
AA Change: R165Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.4e-47	PFAM
Pfam:7tm_1	41	290	4.2e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213335
AA Change: R165Q

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214875

Predicted Effect

probably benign
Transcript: ENSMUST00000215164
AA Change: R165Q

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330161L09Rik	T	C	12: 103,407,589		probably benign	Het
Ablim1	T	C	19: 57,043,813	D638G	possibly damaging	Het
Adam1b	A	G	5: 121,500,714		probably benign	Het
Aim2	T	C	1: 173,459,713	M93T	probably benign	Het
Alpk2	T	C	18: 65,307,080	E414G	possibly damaging	Het
Ap1b1	T	C	11: 5,015,613	F51L	probably damaging	Het
Bard1	T	A	1: 71,075,391	K144*	probably null	Het
Begain	G	T	12: 109,033,917	Y514*	probably null	Het
Ccdc186	A	T	19: 56,800,142	I545N	possibly damaging	Het
Cfap43	T	C	19: 47,835,758	Y58C	probably damaging	Het
Cfap54	A	T	10: 92,886,367	D2437E	unknown	Het
Chd2	A	G	7: 73,429,987	S1722P	probably benign	Het
Clec5a	C	T	6: 40,585,203	G9E	probably damaging	Het
Cog4	A	G	8: 110,858,582	Y292C	possibly damaging	Het
Col3a1	C	T	1: 45,330,145	P331S	unknown	Het
Ctsk	T	C	3: 95,506,677	I245T	probably benign	Het
Dthd1	T	C	5: 62,821,908	Y304H	probably damaging	Het
Dthd1	T	C	5: 62,842,879	S515P	probably damaging	Het
Dtx2	C	T	5: 136,030,577	S493F	probably damaging	Het
Eci2	A	G	13: 34,990,716		probably null	Het
Ecm1	C	T	3: 95,735,942	A349T	probably benign	Het
Efemp2	A	G	19: 5,475,162	E32G	probably damaging	Het
Flt4	C	A	11: 49,625,304	T78K	probably benign	Het
Foxg1	A	G	12: 49,384,925		probably benign	Het
Fv1	T	C	4: 147,870,162	V395A	possibly damaging	Het
Gcfc2	C	A	6: 81,923,778	D24E	probably benign	Het
Gm10696	A	G	3: 94,175,527	S326P	probably damaging	Het
Gpha2	T	G	19: 6,227,502	V96G	probably damaging	Het
Gse1	A	G	8: 120,566,980	Y228C	probably damaging	Het
Hmgxb3	T	C	18: 61,155,386	R470G	possibly damaging	Het
Ildr1	A	G	16: 36,721,979	E247G	probably damaging	Het
Ktn1	T	A	14: 47,693,888	M646K	possibly damaging	Het
Lrrc37a	T	A	11: 103,497,822	H2259L	unknown	Het
Map1b	A	T	13: 99,431,121	H1697Q	unknown	Het
Mdn1	A	G	4: 32,700,409	E1456G	probably damaging	Het
Mta1	C	T	12: 113,131,628	T467I	probably damaging	Het
Nav1	C	T	1: 135,449,004	R1694Q	probably damaging	Het
Ncoa5	C	A	2: 165,012,918	D95Y	possibly damaging	Het
Nfatc1	A	T	18: 80,635,664	C836*	probably null	Het
Nr3c2	T	C	8: 76,908,527	S86P	possibly damaging	Het
Nup153	A	G	13: 46,683,928	S1273P	probably benign	Het
Pcsk5	C	A	19: 17,473,059	G1142C	probably damaging	Het
Pgghg	A	G	7: 140,943,540	D244G	possibly damaging	Het
Ppargc1b	G	A	18: 61,311,250	P297S	probably benign	Het
Rad52	A	T	6: 119,920,894	Q356L	possibly damaging	Het
Rhpn1	T	A	15: 75,713,234	F507I	probably damaging	Het
Rhpn2	T	C	7: 35,377,008	M328T	probably benign	Het
Rnf19a	T	C	15: 36,254,519	I298V	possibly damaging	Het
Rwdd2a	T	C	9: 86,574,131	V120A	probably benign	Het
Scml2	G	T	X: 161,231,446	E566D	possibly damaging	Het
Serpina3a	G	A	12: 104,116,222	A85T	probably damaging	Het
Slc16a11	G	A	11: 70,215,320	G80D	probably damaging	Het
Slc5a5	A	T	8: 70,889,751		probably null	Het
Sparcl1	T	A	5: 104,088,423	Q488L	probably damaging	Het
Spg21	T	A	9: 65,484,429		probably null	Het
Sry	T	C	Y: 2,662,901	H253R	unknown	Het
Swt1	A	G	1: 151,403,885	S391P	probably damaging	Het
Uhrf2	A	G	19: 30,056,488	Y200C	probably damaging	Het
Utp15	T	C	13: 98,254,985	H248R	probably damaging	Het
Utp20	A	G	10: 88,767,451		probably null	Het
Vcan	T	A	13: 89,693,303	D414V	probably damaging	Het
Vmn1r56	A	T	7: 5,196,180	M146K	probably damaging	Het
Wdr66	G	A	5: 123,254,375		probably benign	Het
Zfp143	A	T	7: 110,086,246	K399N	probably damaging	Het
Zfp516	A	G	18: 82,957,411	D578G	probably damaging	Het
Zfp90	G	A	8: 106,425,488	C611Y	probably damaging	Het
Znrd1as	T	C	17: 36,965,444	V306A	possibly damaging	Het

Other mutations in Olfr965

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01705:Olfr965	APN	9	39719581	missense	possibly damaging	0.95
IGL02365:Olfr965	APN	9	39719674	missense	possibly damaging	0.60
IGL02365:Olfr965	APN	9	39720100	missense	probably damaging	0.98
IGL03062:Olfr965	APN	9	39720035	missense	probably benign	0.26
IGL03330:Olfr965	APN	9	39719488	missense	probably benign	0.08
R0011:Olfr965	UTSW	9	39719627	missense	probably benign	0.26
R0462:Olfr965	UTSW	9	39719410	missense	probably benign	0.01
R1505:Olfr965	UTSW	9	39719478	missense	probably damaging	1.00
R1995:Olfr965	UTSW	9	39719413	missense	probably damaging	1.00
R2049:Olfr965	UTSW	9	39720115	missense	probably damaging	1.00
R3817:Olfr965	UTSW	9	39720108	missense	possibly damaging	0.95
R4152:Olfr965	UTSW	9	39720000	missense	probably benign	0.10
R4153:Olfr965	UTSW	9	39720000	missense	probably benign	0.10
R4351:Olfr965	UTSW	9	39719569	missense	probably damaging	0.99
R4377:Olfr965	UTSW	9	39719807	missense	probably benign	0.04
R4667:Olfr965	UTSW	9	39719709	missense	probably benign	0.09
R5526:Olfr965	UTSW	9	39719596	missense	possibly damaging	0.95
R5816:Olfr965	UTSW	9	39719230	start codon destroyed	probably null	1.00
R7113:Olfr965	UTSW	9	39719677	missense	probably benign
R7336:Olfr965	UTSW	9	39719610	missense	probably benign	0.28
R8153:Olfr965	UTSW	9	39719658	missense	possibly damaging	0.68
R8291:Olfr965	UTSW	9	39719545	missense	probably benign	0.00
R8779:Olfr965	UTSW	9	39719340	missense	probably damaging	0.99
R9617:Olfr965	UTSW	9	39719382	missense	possibly damaging	0.80
R9631:Olfr965	UTSW	9	39719865	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- TCCTACCCTGAATGCATGACAC -3'
(R):5'- GGATTCTGGCAATGATAAAGCTG -3'

Sequencing Primer
(F):5'- TGAATGCATGACACAGCTCTAC -3'
(R):5'- CTGTAAGAGCTCAAGATTGTCAGC -3'

Posted On

2014-09-18