Mutagenetix > Incidental Mutations

Incidental Mutation 'R2110:Znrd1as'

232498

Institutional Source

Beutler Lab

Gene Symbol

Znrd1as

Ensembl Gene

ENSMUSG00000036214

Gene Name

zinc ribbon domain containing 1, antisense

Synonyms

1700022C21Rik

MMRRC Submission

040114-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.163) question?

Stock #

R2110 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36958592-36965625 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 36965444 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 306 (V306A)

Ref Sequence

ENSEMBL: ENSMUSP00000147556 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040177] [ENSMUST00000173814] [ENSMUST00000209623]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000040177
AA Change: V236A

PolyPhen 2 Score 0.466 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000048695
Gene: ENSMUSG00000036214
AA Change: V236A

Domain	Start	End	E-Value	Type
low complexity region	98	115	N/A	INTRINSIC
coiled coil region	163	195	N/A	INTRINSIC
low complexity region	224	236	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000119193

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000172672

Predicted Effect

possibly damaging
Transcript: ENSMUST00000173814
AA Change: V157A

PolyPhen 2 Score 0.466 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000134016
Gene: ENSMUSG00000036214
AA Change: V157A

Domain	Start	End	E-Value	Type
low complexity region	19	36	N/A	INTRINSIC
coiled coil region	84	116	N/A	INTRINSIC
low complexity region	145	157	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185085

Predicted Effect

possibly damaging
Transcript: ENSMUST00000209623
AA Change: V306A

PolyPhen 2 Score 0.466 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330161L09Rik	T	C	12: 103,407,589		probably benign	Het
Ablim1	T	C	19: 57,043,813	D638G	possibly damaging	Het
Adam1b	A	G	5: 121,500,714		probably benign	Het
Aim2	T	C	1: 173,459,713	M93T	probably benign	Het
Alpk2	T	C	18: 65,307,080	E414G	possibly damaging	Het
Ap1b1	T	C	11: 5,015,613	F51L	probably damaging	Het
Bard1	T	A	1: 71,075,391	K144*	probably null	Het
Begain	G	T	12: 109,033,917	Y514*	probably null	Het
Ccdc186	A	T	19: 56,800,142	I545N	possibly damaging	Het
Cfap43	T	C	19: 47,835,758	Y58C	probably damaging	Het
Cfap54	A	T	10: 92,886,367	D2437E	unknown	Het
Chd2	A	G	7: 73,429,987	S1722P	probably benign	Het
Clec5a	C	T	6: 40,585,203	G9E	probably damaging	Het
Cog4	A	G	8: 110,858,582	Y292C	possibly damaging	Het
Col3a1	C	T	1: 45,330,145	P331S	unknown	Het
Ctsk	T	C	3: 95,506,677	I245T	probably benign	Het
Dthd1	T	C	5: 62,821,908	Y304H	probably damaging	Het
Dthd1	T	C	5: 62,842,879	S515P	probably damaging	Het
Dtx2	C	T	5: 136,030,577	S493F	probably damaging	Het
Eci2	A	G	13: 34,990,716		probably null	Het
Ecm1	C	T	3: 95,735,942	A349T	probably benign	Het
Efemp2	A	G	19: 5,475,162	E32G	probably damaging	Het
Flt4	C	A	11: 49,625,304	T78K	probably benign	Het
Foxg1	A	G	12: 49,384,925		probably benign	Het
Fv1	T	C	4: 147,870,162	V395A	possibly damaging	Het
Gcfc2	C	A	6: 81,923,778	D24E	probably benign	Het
Gm10696	A	G	3: 94,175,527	S326P	probably damaging	Het
Gpha2	T	G	19: 6,227,502	V96G	probably damaging	Het
Gse1	A	G	8: 120,566,980	Y228C	probably damaging	Het
Hmgxb3	T	C	18: 61,155,386	R470G	possibly damaging	Het
Ildr1	A	G	16: 36,721,979	E247G	probably damaging	Het
Ktn1	T	A	14: 47,693,888	M646K	possibly damaging	Het
Lrrc37a	T	A	11: 103,497,822	H2259L	unknown	Het
Map1b	A	T	13: 99,431,121	H1697Q	unknown	Het
Mdn1	A	G	4: 32,700,409	E1456G	probably damaging	Het
Mta1	C	T	12: 113,131,628	T467I	probably damaging	Het
Nav1	C	T	1: 135,449,004	R1694Q	probably damaging	Het
Ncoa5	C	A	2: 165,012,918	D95Y	possibly damaging	Het
Nfatc1	A	T	18: 80,635,664	C836*	probably null	Het
Nr3c2	T	C	8: 76,908,527	S86P	possibly damaging	Het
Nup153	A	G	13: 46,683,928	S1273P	probably benign	Het
Olfr965	G	A	9: 39,719,722	R165Q	probably benign	Het
Pcsk5	C	A	19: 17,473,059	G1142C	probably damaging	Het
Pgghg	A	G	7: 140,943,540	D244G	possibly damaging	Het
Ppargc1b	G	A	18: 61,311,250	P297S	probably benign	Het
Rad52	A	T	6: 119,920,894	Q356L	possibly damaging	Het
Rhpn1	T	A	15: 75,713,234	F507I	probably damaging	Het
Rhpn2	T	C	7: 35,377,008	M328T	probably benign	Het
Rnf19a	T	C	15: 36,254,519	I298V	possibly damaging	Het
Rwdd2a	T	C	9: 86,574,131	V120A	probably benign	Het
Scml2	G	T	X: 161,231,446	E566D	possibly damaging	Het
Serpina3a	G	A	12: 104,116,222	A85T	probably damaging	Het
Slc16a11	G	A	11: 70,215,320	G80D	probably damaging	Het
Slc5a5	A	T	8: 70,889,751		probably null	Het
Sparcl1	T	A	5: 104,088,423	Q488L	probably damaging	Het
Spg21	T	A	9: 65,484,429		probably null	Het
Sry	T	C	Y: 2,662,901	H253R	unknown	Het
Swt1	A	G	1: 151,403,885	S391P	probably damaging	Het
Uhrf2	A	G	19: 30,056,488	Y200C	probably damaging	Het
Utp15	T	C	13: 98,254,985	H248R	probably damaging	Het
Utp20	A	G	10: 88,767,451		probably null	Het
Vcan	T	A	13: 89,693,303	D414V	probably damaging	Het
Vmn1r56	A	T	7: 5,196,180	M146K	probably damaging	Het
Wdr66	G	A	5: 123,254,375		probably benign	Het
Zfp143	A	T	7: 110,086,246	K399N	probably damaging	Het
Zfp516	A	G	18: 82,957,411	D578G	probably damaging	Het
Zfp90	G	A	8: 106,425,488	C611Y	probably damaging	Het

Other mutations in Znrd1as

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00807:Znrd1as	APN	17	36964921	missense	probably damaging	0.99
R0347:Znrd1as	UTSW	17	36965315	missense	probably damaging	1.00
R0789:Znrd1as	UTSW	17	36964960	missense	probably damaging	1.00
R0993:Znrd1as	UTSW	17	36965047	small deletion	probably benign
R2866:Znrd1as	UTSW	17	36965160	missense	possibly damaging	0.91
R4224:Znrd1as	UTSW	17	36958725	utr 5 prime	probably benign
R4746:Znrd1as	UTSW	17	36964873	missense	probably benign	0.00
R7449:Znrd1as	UTSW	17	36964383	missense	probably damaging	1.00
RF005:Znrd1as	UTSW	17	36965048	small insertion	probably benign
RF008:Znrd1as	UTSW	17	36965054	small insertion	probably benign
RF010:Znrd1as	UTSW	17	36965063	small insertion	probably benign
RF014:Znrd1as	UTSW	17	36965060	small insertion	probably benign
RF024:Znrd1as	UTSW	17	36965057	small insertion	probably benign
RF025:Znrd1as	UTSW	17	36965048	small insertion	probably benign
RF029:Znrd1as	UTSW	17	36965071	small insertion	probably benign
RF035:Znrd1as	UTSW	17	36965066	small insertion	probably benign
RF046:Znrd1as	UTSW	17	36965057	small insertion	probably benign
RF048:Znrd1as	UTSW	17	36965059	small insertion	probably benign
RF053:Znrd1as	UTSW	17	36965066	small insertion	probably benign
RF064:Znrd1as	UTSW	17	36965050	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- ACAGCCACGACTCTTCAGTG -3'
(R):5'- TCGCAGAAGAAACATGGTACC -3'

Sequencing Primer
(F):5'- ACTACCCAAAATTGTGCTAGAGG -3'
(R):5'- CATGGTACCATTAGCAAAGCTCTG -3'

Posted On

2014-09-18