Mutagenetix > Incidental Mutation

Incidental Mutation 'R0194:Helz2'

23264

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

038453-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0194 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 181232759 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 1981 (G1981C)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094203
AA Change: G1981C

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: G1981C

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108831
AA Change: G1981C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: G1981C

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121484
AA Change: G1981C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: G1981C

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149417

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155049

Meta Mutation Damage Score

0.4696

Coding Region Coverage

1x: 98.6%
3x: 97.3%
10x: 91.4%
20x: 70.1%

Validation Efficiency

91% (439/482)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 102 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110043O21Rik	T	A	4: 35,197,207 (GRCm38)	D122V	probably damaging	Het
4930553M12Rik	T	A	4: 88,868,243 (GRCm38)	D46V	unknown	Het
Abcb9	A	G	5: 124,077,295 (GRCm38)	V461A	probably damaging	Het
Ackr4	T	A	9: 104,099,480 (GRCm38)	L89F	probably benign	Het
Acsf2	T	C	11: 94,561,370 (GRCm38)	T449A	probably benign	Het
Acsl4	C	G	X: 142,333,718 (GRCm38)	G489R	probably damaging	Het
Actl6a	T	A	3: 32,725,320 (GRCm38)	I399N	probably damaging	Het
Adamts19	G	A	18: 59,011,148 (GRCm38)	C934Y	probably null	Het
Adsl	A	G	15: 80,961,360 (GRCm38)	E40G	possibly damaging	Het
AI481877	T	A	4: 59,066,534 (GRCm38)		probably benign	Het
Alppl2	T	G	1: 87,088,743 (GRCm38)	D203A	probably damaging	Het
Asb10	C	A	5: 24,537,932 (GRCm38)	A268S	probably benign	Het
Atp9a	T	C	2: 168,643,885 (GRCm38)	S832G	probably benign	Het
Bckdha	A	T	7: 25,631,450 (GRCm38)	I297N	probably damaging	Het
Blm	G	A	7: 80,464,946 (GRCm38)		probably benign	Het
Cacna1h	A	G	17: 25,380,924 (GRCm38)		probably benign	Het
Camsap2	G	A	1: 136,292,948 (GRCm38)	Q298*	probably null	Het
Ccdc38	A	T	10: 93,565,912 (GRCm38)	K145*	probably null	Het
Cfap45	C	T	1: 172,541,327 (GRCm38)	T434M	probably benign	Het
Cfap54	A	T	10: 93,034,662 (GRCm38)		probably benign	Het
Clcn6	G	A	4: 148,012,756 (GRCm38)	P618L	probably damaging	Het
Copg1	T	C	6: 87,904,197 (GRCm38)		probably benign	Het
Dctd	T	A	8: 48,112,078 (GRCm38)	N79K	probably benign	Het
Dgkq	A	G	5: 108,654,644 (GRCm38)		probably benign	Het
Dntt	A	T	19: 41,038,970 (GRCm38)	T159S	possibly damaging	Het
Doc2g	G	A	19: 4,003,656 (GRCm38)	R29Q	probably benign	Het
Dsg3	A	G	18: 20,540,142 (GRCm38)	T957A	probably damaging	Het
Eif3c	T	A	7: 126,558,623 (GRCm38)		probably benign	Het
Ephb3	T	A	16: 21,218,109 (GRCm38)	D107E	probably benign	Het
Esrrb	A	T	12: 86,470,481 (GRCm38)	D108V	probably damaging	Het
Exo1	A	G	1: 175,892,030 (GRCm38)	K214E	probably damaging	Het
Fam186a	G	A	15: 99,941,763 (GRCm38)	T2200I	possibly damaging	Het
Fam227a	C	T	15: 79,640,669 (GRCm38)	W194*	probably null	Het
Foxn4	A	G	5: 114,259,748 (GRCm38)		probably null	Het
Gabbr2	T	C	4: 46,787,565 (GRCm38)	K366R	possibly damaging	Het
Garem2	T	A	5: 30,113,930 (GRCm38)	V130E	probably damaging	Het
Grin2b	A	G	6: 135,779,305 (GRCm38)	F474S	probably damaging	Het
H2-M10.6	G	T	17: 36,814,042 (GRCm38)	V284F	probably damaging	Het
Hivep1	G	T	13: 42,155,435 (GRCm38)	V384F	probably damaging	Het
Hmox1	A	G	8: 75,097,108 (GRCm38)	T135A	probably damaging	Het
Hpse	T	C	5: 100,719,512 (GRCm38)	D28G	probably benign	Het
Itm2b	G	T	14: 73,364,618 (GRCm38)	D213E	probably benign	Het
Jakmip1	T	A	5: 37,134,283 (GRCm38)	M692K	possibly damaging	Het
Kdm3a	T	C	6: 71,624,594 (GRCm38)	Q151R	probably null	Het
Limch1	C	A	5: 66,999,273 (GRCm38)	A517E	probably benign	Het
Lrit1	T	A	14: 37,061,720 (GRCm38)	L335Q	probably damaging	Het
Lrrc37a	A	G	11: 103,499,790 (GRCm38)	V1603A	possibly damaging	Het
Mbtps1	T	A	8: 119,535,369 (GRCm38)	N347I	probably damaging	Het
Mier1	A	T	4: 103,139,519 (GRCm38)		probably null	Het
Mt2	A	T	8: 94,172,848 (GRCm38)	M1L	probably damaging	Het
Mug1	A	T	6: 121,840,107 (GRCm38)	E45V	probably damaging	Het
Mybphl	A	G	3: 108,374,168 (GRCm38)	K67E	probably benign	Het
Myh4	A	G	11: 67,252,336 (GRCm38)	K1030R	probably damaging	Het
Myl3	T	A	9: 110,769,121 (GRCm38)	D176E	probably benign	Het
Ncapg2	A	G	12: 116,420,683 (GRCm38)		probably null	Het
Ndor1	T	C	2: 25,248,706 (GRCm38)		probably null	Het
Nedd4	T	G	9: 72,670,053 (GRCm38)	N53K	possibly damaging	Het
Nek11	C	A	9: 105,392,952 (GRCm38)	A24S	probably benign	Het
Nudt19	G	T	7: 35,551,514 (GRCm38)	P267T	probably benign	Het
Olfml2b	T	C	1: 170,681,115 (GRCm38)	M514T	possibly damaging	Het
Olfr304	A	T	7: 86,386,374 (GRCm38)	C95*	probably null	Het
Olfr424	A	T	1: 174,136,761 (GRCm38)	T6S	probably benign	Het
Olfr556	A	G	7: 102,670,199 (GRCm38)	D93G	probably benign	Het
Olfr699	C	A	7: 106,790,823 (GRCm38)	M59I	probably benign	Het
P3h1	T	A	4: 119,237,952 (GRCm38)	F302Y	probably damaging	Het
Pappa2	T	A	1: 158,765,101 (GRCm38)		probably benign	Het
Pex2	A	C	3: 5,561,364 (GRCm38)	H128Q	probably benign	Het
Phf11d	A	C	14: 59,352,731 (GRCm38)	L214R	probably damaging	Het
Plcg2	G	A	8: 117,573,397 (GRCm38)		probably benign	Het
Ppargc1b	A	C	18: 61,307,945 (GRCm38)	L634R	possibly damaging	Het
Prune1	A	T	3: 95,262,360 (GRCm38)	I177N	probably damaging	Het
Puf60	T	C	15: 76,070,485 (GRCm38)	D496G	probably damaging	Het
Rasl11b	A	G	5: 74,196,163 (GRCm38)		probably null	Het
Sdr42e1	A	T	8: 117,663,109 (GRCm38)	F264L	probably damaging	Het
Sec24b	A	T	3: 129,984,165 (GRCm38)		probably null	Het
Sgta	G	T	10: 81,051,059 (GRCm38)	P79T	probably benign	Het
Shisa9	C	T	16: 11,984,954 (GRCm38)	T125M	probably damaging	Het
Slc12a2	A	G	18: 57,930,211 (GRCm38)	D921G	probably damaging	Het
Slc13a5	T	A	11: 72,262,130 (GRCm38)	I42L	possibly damaging	Het
Slc13a5	C	T	11: 72,245,233 (GRCm38)	V494I	probably benign	Het
Spire2	G	A	8: 123,363,011 (GRCm38)		probably benign	Het
Sptbn4	G	A	7: 27,404,911 (GRCm38)	R962C	probably benign	Het
St8sia5	G	A	18: 77,254,724 (GRCm38)	V377I	probably benign	Het
Stag2	T	G	X: 42,206,137 (GRCm38)		probably benign	Het
Syne1	C	A	10: 5,424,311 (GRCm38)	M165I	probably benign	Het
Synm	C	A	7: 67,734,924 (GRCm38)	V997L	probably damaging	Het
Tacc1	A	G	8: 25,182,376 (GRCm38)	S279P	probably benign	Het
Tbc1d10a	T	C	11: 4,212,901 (GRCm38)		probably null	Het
Tbc1d19	A	G	5: 53,860,156 (GRCm38)	T302A	probably damaging	Het
Tecpr1	A	C	5: 144,218,517 (GRCm38)	N74K	probably damaging	Het
Tmem120a	T	C	5: 135,742,398 (GRCm38)	E28G	possibly damaging	Het
Tnfrsf1b	A	T	4: 145,224,812 (GRCm38)	I186N	probably benign	Het
Trim55	A	G	3: 19,661,861 (GRCm38)	D195G	probably benign	Het
Trpm3	G	T	19: 22,715,356 (GRCm38)		probably null	Het
Ttc39a	T	A	4: 109,444,179 (GRCm38)	S571T	probably benign	Het
Vwf	T	G	6: 125,643,297 (GRCm38)	I1646S	probably benign	Het
Wbp2nl	T	C	15: 82,314,282 (GRCm38)	F340S	possibly damaging	Het
Yeats2	T	C	16: 20,152,969 (GRCm38)	M1T	probably null	Het
Zfp236	T	A	18: 82,656,987 (GRCm38)	E460V	probably damaging	Het
Zfp277	G	A	12: 40,378,877 (GRCm38)		probably benign	Het
Zfp975	T	A	7: 42,662,492 (GRCm38)	K232N	probably benign	Het
Zxdc	T	C	6: 90,372,537 (GRCm38)		probably benign	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181,229,702 (GRCm38)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181,233,006 (GRCm38)	nonsense	probably null
IGL00704:Helz2	APN	2	181,234,385 (GRCm38)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181,232,245 (GRCm38)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181,233,977 (GRCm38)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181,232,881 (GRCm38)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181,238,481 (GRCm38)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181,232,185 (GRCm38)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181,231,690 (GRCm38)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181,230,911 (GRCm38)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181,231,022 (GRCm38)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181,235,026 (GRCm38)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181,231,146 (GRCm38)	intron	probably benign
IGL03003:Helz2	APN	2	181,240,253 (GRCm38)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181,229,222 (GRCm38)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181,231,804 (GRCm38)	missense	probably benign	0.00
Colby	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181,234,834 (GRCm38)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181,240,959 (GRCm38)	missense	probably benign
R0013:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181,237,802 (GRCm38)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181,232,269 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181,230,593 (GRCm38)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181,232,209 (GRCm38)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181,229,656 (GRCm38)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181,227,770 (GRCm38)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181,234,825 (GRCm38)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181,232,089 (GRCm38)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181,232,116 (GRCm38)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181,240,881 (GRCm38)	missense	probably benign
R0826:Helz2	UTSW	2	181,240,853 (GRCm38)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181,230,777 (GRCm38)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181,236,135 (GRCm38)	missense	probably benign
R1170:Helz2	UTSW	2	181,229,815 (GRCm38)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181,231,128 (GRCm38)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181,237,596 (GRCm38)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181,232,981 (GRCm38)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181,235,524 (GRCm38)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181,232,804 (GRCm38)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181,233,228 (GRCm38)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181,234,147 (GRCm38)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181,236,263 (GRCm38)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181,238,459 (GRCm38)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	181,234,987 (GRCm38)	missense	probably benign
R1837:Helz2	UTSW	2	181,229,289 (GRCm38)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181,232,085 (GRCm38)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181,234,289 (GRCm38)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181,233,750 (GRCm38)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	181,231,329 (GRCm38)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	181,232,578 (GRCm38)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181,237,479 (GRCm38)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181,240,544 (GRCm38)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181,235,102 (GRCm38)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181,231,380 (GRCm38)	missense	probably benign
R2180:Helz2	UTSW	2	181,233,732 (GRCm38)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181,229,048 (GRCm38)	nonsense	probably null
R2248:Helz2	UTSW	2	181,233,433 (GRCm38)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181,232,912 (GRCm38)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181,240,742 (GRCm38)	missense	probably benign
R3617:Helz2	UTSW	2	181,233,061 (GRCm38)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181,240,389 (GRCm38)	nonsense	probably null
R3803:Helz2	UTSW	2	181,239,996 (GRCm38)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181,229,710 (GRCm38)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181,240,475 (GRCm38)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181,229,902 (GRCm38)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181,228,833 (GRCm38)	missense	probably benign
R4624:Helz2	UTSW	2	181,239,308 (GRCm38)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181,238,417 (GRCm38)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181,237,417 (GRCm38)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181,230,120 (GRCm38)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181,236,147 (GRCm38)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181,232,438 (GRCm38)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181,240,916 (GRCm38)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181,240,569 (GRCm38)	missense	probably benign
R5089:Helz2	UTSW	2	181,235,149 (GRCm38)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181,230,757 (GRCm38)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181,234,846 (GRCm38)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181,235,528 (GRCm38)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R5559:Helz2	UTSW	2	181,230,126 (GRCm38)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181,240,258 (GRCm38)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181,237,289 (GRCm38)	intron	probably benign
R5805:Helz2	UTSW	2	181,240,508 (GRCm38)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181,236,396 (GRCm38)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181,232,656 (GRCm38)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181,234,028 (GRCm38)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181,230,384 (GRCm38)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181,230,767 (GRCm38)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181,231,050 (GRCm38)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181,240,313 (GRCm38)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181,233,038 (GRCm38)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181,232,294 (GRCm38)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181,235,945 (GRCm38)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R6581:Helz2	UTSW	2	181,229,379 (GRCm38)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181,239,557 (GRCm38)	nonsense	probably null
R6964:Helz2	UTSW	2	181,230,428 (GRCm38)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181,240,514 (GRCm38)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181,231,285 (GRCm38)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181,238,423 (GRCm38)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181,235,600 (GRCm38)	splice site	probably null
R7512:Helz2	UTSW	2	181,230,854 (GRCm38)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	181,237,572 (GRCm38)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	181,231,996 (GRCm38)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181,230,355 (GRCm38)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181,234,531 (GRCm38)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181,233,991 (GRCm38)	missense	probably benign
R7799:Helz2	UTSW	2	181,237,989 (GRCm38)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181,232,902 (GRCm38)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181,237,750 (GRCm38)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181,240,205 (GRCm38)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	181,237,896 (GRCm38)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	181,238,262 (GRCm38)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181,238,102 (GRCm38)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181,230,157 (GRCm38)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181,229,557 (GRCm38)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181,232,767 (GRCm38)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181,233,127 (GRCm38)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181,238,380 (GRCm38)	missense
R8953:Helz2	UTSW	2	181,233,091 (GRCm38)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181,229,614 (GRCm38)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181,237,788 (GRCm38)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181,234,693 (GRCm38)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181,228,999 (GRCm38)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181,232,468 (GRCm38)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181,240,175 (GRCm38)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181,239,640 (GRCm38)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181,240,055 (GRCm38)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181,230,090 (GRCm38)	missense	probably benign
R9186:Helz2	UTSW	2	181,234,664 (GRCm38)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181,240,948 (GRCm38)	missense	probably benign
R9407:Helz2	UTSW	2	181,240,182 (GRCm38)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181,232,917 (GRCm38)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181,236,452 (GRCm38)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181,240,221 (GRCm38)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181,240,677 (GRCm38)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181,240,232 (GRCm38)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181,240,823 (GRCm38)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181,231,741 (GRCm38)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181,237,564 (GRCm38)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181,235,961 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGATCAAGACTCGTGCCCAGAAG -3'
(R):5'- AGCAACCTGCTGTTCACCACTC -3'

Sequencing Primer
(F):5'- TCGTGCCCAGAAGTGGTAAAG -3'
(R):5'- TTGGTACACATGGGCAACCTC -3'

Posted On

2013-04-16