Mutagenetix > Incidental Mutation

Incidental Mutation 'R2113:Trrap'

232762

Institutional Source

Beutler Lab

Gene Symbol

Trrap

Ensembl Gene

ENSMUSG00000045482

Gene Name

transformation/transcription domain-associated protein

Synonyms

transactivation/transformation-domain associated protein

MMRRC Submission

040117-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2113 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144704547-144796588 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 144781021 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 3261 (Y3261N)

Ref Sequence

ENSEMBL: ENSMUSP00000148419 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038980] [ENSMUST00000094120] [ENSMUST00000100467] [ENSMUST00000213013]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000038980
AA Change: Y3231N

PolyPhen 2 Score 0.669 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000042544
Gene: ENSMUSG00000045482
AA Change: Y3231N

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3363	3376	N/A	INTRINSIC
low complexity region	3407	3418	N/A	INTRINSIC
PI3Kc	3509	3798	5.11e-8	SMART
FATC	3797	3829	1.89e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094120
AA Change: Y3260N

PolyPhen 2 Score 0.473 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000091668
Gene: ENSMUSG00000045482
AA Change: Y3260N

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1682	2e-6	SMART
low complexity region	1850	1861	N/A	INTRINSIC
low complexity region	1884	1899	N/A	INTRINSIC
low complexity region	2307	2321	N/A	INTRINSIC
Pfam:FAT	2848	3203	1.1e-68	PFAM
low complexity region	3392	3405	N/A	INTRINSIC
low complexity region	3436	3447	N/A	INTRINSIC
PI3Kc	3538	3827	5.11e-8	SMART
FATC	3826	3858	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000100467
AA Change: Y3231N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098035
Gene: ENSMUSG00000045482
AA Change: Y3231N

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3381	3394	N/A	INTRINSIC
low complexity region	3425	3436	N/A	INTRINSIC
PI3Kc	3527	3816	5.11e-8	SMART
FATC	3815	3847	1.89e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124909

Predicted Effect

SMART Domains

Protein: ENSMUSP00000122021
Gene: ENSMUSG00000045482
AA Change: Y2970N

Domain	Start	End	E-Value	Type
low complexity region	197	242	N/A	INTRINSIC
low complexity region	244	255	N/A	INTRINSIC
SCOP:d1gw5a_	474	1003	9e-7	SMART
Blast:PI3Kc	480	579	1e-13	BLAST
low complexity region	1083	1092	N/A	INTRINSIC
low complexity region	1572	1583	N/A	INTRINSIC
low complexity region	1606	1621	N/A	INTRINSIC
low complexity region	2029	2043	N/A	INTRINSIC
Pfam:FAT	2570	2914	1.5e-69	PFAM
low complexity region	3121	3134	N/A	INTRINSIC
low complexity region	3165	3176	N/A	INTRINSIC
PI3Kc	3267	3556	5.11e-8	SMART
FATC	3555	3587	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000213013
AA Change: Y3261N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygous embryos die prior to E3.5 and exhibit embryonic and extraembryonic tissue disorganization. Mitotic abnormalities were also noted in homozygous cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 127 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd4	C	A	12: 84,655,790 (GRCm39)	G295*	probably null	Het
Acad9	T	C	3: 36,128,525 (GRCm39)	Y129H	probably damaging	Het
Actn4	C	T	7: 28,597,549 (GRCm39)	G608D	probably benign	Het
Adcyap1r1	T	A	6: 55,458,100 (GRCm39)	N300K	probably damaging	Het
Adh5	A	G	3: 138,157,245 (GRCm39)	I269V	probably benign	Het
Afap1l2	G	A	19: 56,901,821 (GRCm39)	A842V	possibly damaging	Het
AI429214	A	T	8: 37,461,154 (GRCm39)	K101*	probably null	Het
Alg10b	T	A	15: 90,109,860 (GRCm39)	W58R	probably damaging	Het
Alpk2	C	T	18: 65,438,754 (GRCm39)	E1347K	probably benign	Het
Amotl2	G	T	9: 102,601,922 (GRCm39)	E389*	probably null	Het
Ap5m1	G	A	14: 49,323,705 (GRCm39)	R465Q	probably damaging	Het
Apoa1	A	C	9: 46,140,512 (GRCm39)	S48R	probably damaging	Het
Asb1	T	C	1: 91,471,950 (GRCm39)	L34P	probably damaging	Het
Atg14	C	T	14: 47,788,781 (GRCm39)	A191T	probably damaging	Het
Atp13a4	C	T	16: 29,260,102 (GRCm39)	V235I	possibly damaging	Het
Atrnl1	C	T	19: 57,744,048 (GRCm39)	Q1217*	probably null	Het
Bltp2	T	C	11: 78,159,598 (GRCm39)	V549A	probably benign	Het
Bsn	A	T	9: 107,992,085 (GRCm39)	H1222Q	probably benign	Het
Catsper4	A	T	4: 133,945,552 (GRCm39)	V169E	probably damaging	Het
Cbx4	C	T	11: 118,972,718 (GRCm39)	G219E	probably damaging	Het
Ccdc136	C	T	6: 29,413,031 (GRCm39)	T406I	possibly damaging	Het
Celsr3	A	T	9: 108,715,669 (GRCm39)	Y2117F	probably damaging	Het
Cenpf	T	A	1: 189,411,299 (GRCm39)	Q295L	probably damaging	Het
Ces1b	A	G	8: 93,794,783 (GRCm39)	V272A	probably benign	Het
Cgn	A	T	3: 94,687,116 (GRCm39)	V62D	probably damaging	Het
Ckap4	T	G	10: 84,369,387 (GRCm39)	Q115P	possibly damaging	Het
Clec10a	T	C	11: 70,060,650 (GRCm39)		probably null	Het
Clmn	A	T	12: 104,747,067 (GRCm39)	S827T	probably benign	Het
Cntn4	T	C	6: 106,466,658 (GRCm39)	S187P	probably damaging	Het
Cntnap5a	A	T	1: 116,116,095 (GRCm39)	I526F	probably damaging	Het
Cntnap5b	A	G	1: 100,202,140 (GRCm39)	Q329R	probably benign	Het
Col1a1	T	A	11: 94,839,188 (GRCm39)	S979T	unknown	Het
Crat	T	A	2: 30,292,654 (GRCm39)	Y606F	probably benign	Het
Cyp2c29	G	T	19: 39,318,708 (GRCm39)	C396F	probably damaging	Het
Cyp2d34	G	T	15: 82,501,817 (GRCm39)	P231Q	probably damaging	Het
Ddi2	A	T	4: 141,430,591 (GRCm39)		probably null	Het
Dicer1	A	T	12: 104,679,473 (GRCm39)	H501Q	probably damaging	Het
Dnah12	A	G	14: 26,488,098 (GRCm39)	I1001V	probably damaging	Het
Dnase2a	T	A	8: 85,637,500 (GRCm39)	C301S	probably damaging	Het
Dock10	T	A	1: 80,584,280 (GRCm39)	D142V	probably damaging	Het
Dpp8	G	A	9: 64,971,150 (GRCm39)	C590Y	probably benign	Het
Dst	T	A	1: 34,314,317 (GRCm39)	M6707K	probably damaging	Het
Duox1	T	C	2: 122,167,735 (GRCm39)	V1006A	probably benign	Het
Dync1h1	T	C	12: 110,596,420 (GRCm39)	S1623P	probably damaging	Het
Ehmt1	A	T	2: 24,694,015 (GRCm39)	D1011E	probably damaging	Het
Epc2	T	A	2: 49,422,235 (GRCm39)	D376E	probably benign	Het
Eps8	C	A	6: 137,514,633 (GRCm39)		probably null	Het
F11	T	C	8: 45,699,869 (GRCm39)	T406A	probably benign	Het
Fat3	T	G	9: 15,911,082 (GRCm39)	D1640A	probably damaging	Het
Fbxo15	A	T	18: 84,977,230 (GRCm39)	R47S	probably benign	Het
Fbxo28	A	T	1: 182,157,490 (GRCm39)	V109E	probably damaging	Het
Fn1	A	T	1: 71,665,323 (GRCm39)	S931R	probably damaging	Het
Fndc3b	T	C	3: 27,697,185 (GRCm39)	D9G	probably damaging	Het
Frem2	G	A	3: 53,560,343 (GRCm39)	T1388I	probably damaging	Het
Fstl3	C	A	10: 79,617,012 (GRCm39)	T185N	probably damaging	Het
Glo1	A	T	17: 30,823,014 (GRCm39)	Y49*	probably null	Het
Gtsf2	T	C	15: 103,348,100 (GRCm39)	M137V	probably benign	Het
Gzmk	C	T	13: 113,310,489 (GRCm39)	G110S	probably benign	Het
Hdac9	G	T	12: 34,439,331 (GRCm39)	S428R	probably damaging	Het
Hnrnpul1	G	A	7: 25,432,694 (GRCm39)	T456I	possibly damaging	Het
Ino80	T	A	2: 119,284,565 (GRCm39)	H172L	probably damaging	Het
Itgb8	A	C	12: 119,154,347 (GRCm39)	L230R	probably damaging	Het
Klf9	C	T	19: 23,142,052 (GRCm39)	R171W	probably damaging	Het
Kmt2b	G	T	7: 30,282,812 (GRCm39)	P1050Q	probably damaging	Het
Krt15	A	T	11: 100,026,484 (GRCm39)	F67L	unknown	Het
Maml3	T	A	3: 51,598,077 (GRCm39)	Y223F	probably damaging	Het
Mcm9	C	T	10: 53,491,943 (GRCm39)		probably null	Het
Mettl3	A	G	14: 52,532,441 (GRCm39)	*104Q	probably null	Het
Msrb3	T	A	10: 120,687,985 (GRCm39)	D30V	possibly damaging	Het
Mycbp2	T	C	14: 103,457,512 (GRCm39)	T1562A	probably damaging	Het
Nat14	T	C	7: 4,927,038 (GRCm39)	V70A	possibly damaging	Het
Nbeal2	T	C	9: 110,454,474 (GRCm39)	T2685A	probably damaging	Het
Nek1	A	G	8: 61,469,327 (GRCm39)	D128G	probably damaging	Het
Nfam1	T	A	15: 82,899,202 (GRCm39)	K155*	probably null	Het
Noc4l	A	T	5: 110,798,425 (GRCm39)	M255K	possibly damaging	Het
Nolc1	GCA	GCAACA	19: 46,069,800 (GRCm39)		probably benign	Het
Nolc1	CAG	CAGGAG	19: 46,069,798 (GRCm39)		probably benign	Het
Nprl2	T	C	9: 107,422,511 (GRCm39)	S334P	probably benign	Het
Nsrp1	A	G	11: 76,937,396 (GRCm39)	S267P	probably benign	Het
Ntn4	A	T	10: 93,480,701 (GRCm39)	M142L	probably damaging	Het
Nup188	T	A	2: 30,194,113 (GRCm39)	C139*	probably null	Het
Nup210l	A	T	3: 90,098,281 (GRCm39)	N1411I	possibly damaging	Het
Or10g1	A	G	14: 52,647,753 (GRCm39)	L192P	probably damaging	Het
Or5ak24	T	G	2: 85,260,430 (GRCm39)	T248P	probably damaging	Het
Pard3	A	T	8: 128,115,018 (GRCm39)	T579S	probably damaging	Het
Pcnx3	G	T	19: 5,721,584 (GRCm39)	D1071E	possibly damaging	Het
Pi4k2a	T	A	19: 42,103,510 (GRCm39)	I340N	possibly damaging	Het
Pkdrej	T	A	15: 85,703,185 (GRCm39)	D917V	probably damaging	Het
Plekhg4	T	A	8: 106,106,066 (GRCm39)	F821Y	probably damaging	Het
Ptgfrn	C	T	3: 100,984,625 (GRCm39)	R189H	probably benign	Het
Ptpn18	T	A	1: 34,510,742 (GRCm39)	S235R	probably damaging	Het
Rabep2	A	G	7: 126,044,460 (GRCm39)		probably null	Het
Rbp3	T	G	14: 33,678,014 (GRCm39)	I654S	probably benign	Het
Rimbp3	T	C	16: 17,027,539 (GRCm39)	L321P	probably benign	Het
Rims2	T	A	15: 39,374,722 (GRCm39)	M1028K	probably benign	Het
Ruvbl1	T	A	6: 88,460,003 (GRCm39)	V221D	probably damaging	Het
Ruvbl2	A	T	7: 45,073,527 (GRCm39)		probably null	Het
Scn7a	C	T	2: 66,506,312 (GRCm39)	D1526N	probably damaging	Het
Scnn1a	T	C	6: 125,314,774 (GRCm39)	F218S	possibly damaging	Het
Sh3tc2	T	A	18: 62,146,176 (GRCm39)	M1185K	probably damaging	Het
Slain1	C	A	14: 103,888,282 (GRCm39)	D67E	possibly damaging	Het
Snx33	T	C	9: 56,833,724 (GRCm39)	D115G	probably benign	Het
Sowaha	G	A	11: 53,369,789 (GRCm39)	R316C	probably damaging	Het
Spata17	A	T	1: 186,830,108 (GRCm39)	F309I	possibly damaging	Het
Spmip7	G	A	11: 11,440,293 (GRCm39)		probably null	Het
Ssrp1	G	T	2: 84,873,350 (GRCm39)		probably null	Het
St6gal1	C	G	16: 23,147,167 (GRCm39)	T225S	probably damaging	Het
Sulf1	C	G	1: 12,918,398 (GRCm39)	F38L	probably damaging	Het
Tbc1d7	G	A	13: 43,306,562 (GRCm39)	T138M	probably damaging	Het
Tdpoz4	T	A	3: 93,704,351 (GRCm39)	M216K	probably damaging	Het
Tmem200a	A	G	10: 25,869,220 (GRCm39)	S350P	probably damaging	Het
Tnpo3	A	T	6: 29,551,871 (GRCm39)	D903E	probably benign	Het
Trpc4ap	A	T	2: 155,499,856 (GRCm39)	I222N	probably damaging	Het
Ttn	A	G	2: 76,542,954 (GRCm39)	V33344A	possibly damaging	Het
Uchl4	A	T	9: 64,142,818 (GRCm39)	T100S	probably damaging	Het
Uxs1	A	G	1: 43,810,933 (GRCm39)	Y97H	probably damaging	Het
Vmn1r192	A	T	13: 22,371,800 (GRCm39)	V140D	possibly damaging	Het
Vmn2r114	ATTT	ATT	17: 23,509,906 (GRCm39)		probably null	Het
Vmn2r7	A	G	3: 64,599,025 (GRCm39)	S511P	possibly damaging	Het
Vmn2r88	T	C	14: 51,655,651 (GRCm39)	L621P	probably damaging	Het
Vps45	A	T	3: 95,954,365 (GRCm39)	M174K	probably benign	Het
Wdr72	A	T	9: 74,052,454 (GRCm39)	M162L	probably benign	Het
Wdr81	T	A	11: 75,344,461 (GRCm39)	M269L	probably benign	Het
Zfp53	A	G	17: 21,728,713 (GRCm39)	T249A	probably benign	Het
Zfp758	T	C	17: 22,580,626 (GRCm39)	S22P	probably benign	Het
Zfp780b	T	C	7: 27,663,298 (GRCm39)	D419G	possibly damaging	Het
Zfp934	A	T	13: 62,666,507 (GRCm39)	Y45N	probably damaging	Het

Other mutations in Trrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Trrap	APN	5	144,716,784 (GRCm39)	splice site	probably benign
IGL00470:Trrap	APN	5	144,754,848 (GRCm39)	missense	probably damaging	1.00
IGL00490:Trrap	APN	5	144,762,035 (GRCm39)	missense	probably benign	0.40
IGL01072:Trrap	APN	5	144,721,065 (GRCm39)	splice site	probably benign
IGL01087:Trrap	APN	5	144,783,349 (GRCm39)	missense	probably damaging	0.99
IGL01300:Trrap	APN	5	144,741,628 (GRCm39)	missense	probably damaging	1.00
IGL01350:Trrap	APN	5	144,767,779 (GRCm39)	missense	possibly damaging	0.92
IGL01410:Trrap	APN	5	144,767,831 (GRCm39)	missense	probably benign	0.00
IGL01571:Trrap	APN	5	144,770,097 (GRCm39)	splice site	probably benign
IGL01748:Trrap	APN	5	144,770,150 (GRCm39)	missense	probably damaging	1.00
IGL01839:Trrap	APN	5	144,758,685 (GRCm39)	missense	probably damaging	1.00
IGL01976:Trrap	APN	5	144,793,799 (GRCm39)	missense	probably benign	0.00
IGL02075:Trrap	APN	5	144,765,304 (GRCm39)	missense	probably benign	0.00
IGL02127:Trrap	APN	5	144,753,243 (GRCm39)	missense	probably benign	0.22
IGL02131:Trrap	APN	5	144,777,246 (GRCm39)	missense	probably damaging	1.00
IGL02287:Trrap	APN	5	144,769,348 (GRCm39)	missense	probably damaging	1.00
IGL02301:Trrap	APN	5	144,714,727 (GRCm39)	missense	probably benign	0.05
IGL02336:Trrap	APN	5	144,735,200 (GRCm39)	missense	probably benign	0.39
IGL02526:Trrap	APN	5	144,761,360 (GRCm39)	missense	probably benign	0.00
IGL02873:Trrap	APN	5	144,777,889 (GRCm39)	splice site	probably benign
IGL02953:Trrap	APN	5	144,752,774 (GRCm39)	missense	probably damaging	0.99
IGL03404:Trrap	APN	5	144,769,996 (GRCm39)	missense	probably benign	0.00
Buffer	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
Card-tower	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
Cookie	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
Glass_house	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
Immovable	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R5049_trrap_520	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R7167_Trrap_977	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
vitreous	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
PIT4243001:Trrap	UTSW	5	144,733,781 (GRCm39)	missense	probably benign	0.00
PIT4466001:Trrap	UTSW	5	144,765,410 (GRCm39)	missense	probably benign	0.02
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0062:Trrap	UTSW	5	144,719,003 (GRCm39)	splice site	probably benign
R0112:Trrap	UTSW	5	144,759,571 (GRCm39)	nonsense	probably null
R0126:Trrap	UTSW	5	144,742,560 (GRCm39)	nonsense	probably null
R0257:Trrap	UTSW	5	144,741,045 (GRCm39)	missense	probably benign	0.31
R0325:Trrap	UTSW	5	144,753,205 (GRCm39)	missense	probably benign	0.05
R0376:Trrap	UTSW	5	144,753,149 (GRCm39)	missense	probably benign	0.03
R0396:Trrap	UTSW	5	144,751,366 (GRCm39)	missense	probably damaging	0.99
R0448:Trrap	UTSW	5	144,776,377 (GRCm39)	missense	possibly damaging	0.66
R0454:Trrap	UTSW	5	144,783,287 (GRCm39)	missense	probably damaging	1.00
R0711:Trrap	UTSW	5	144,790,309 (GRCm39)	missense	probably damaging	1.00
R0827:Trrap	UTSW	5	144,751,640 (GRCm39)	missense	probably benign	0.00
R1005:Trrap	UTSW	5	144,742,537 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,741,576 (GRCm39)	missense	probably damaging	1.00
R1179:Trrap	UTSW	5	144,714,749 (GRCm39)	missense	possibly damaging	0.94
R1218:Trrap	UTSW	5	144,753,219 (GRCm39)	missense	probably damaging	1.00
R1264:Trrap	UTSW	5	144,726,409 (GRCm39)	splice site	probably benign
R1374:Trrap	UTSW	5	144,783,428 (GRCm39)	missense	probably damaging	1.00
R1401:Trrap	UTSW	5	144,794,232 (GRCm39)	missense	possibly damaging	0.93
R1480:Trrap	UTSW	5	144,755,123 (GRCm39)	missense	probably benign
R1538:Trrap	UTSW	5	144,774,012 (GRCm39)	missense	possibly damaging	0.65
R1751:Trrap	UTSW	5	144,751,385 (GRCm39)	critical splice donor site	probably null
R1779:Trrap	UTSW	5	144,765,400 (GRCm39)	missense	probably benign	0.01
R1782:Trrap	UTSW	5	144,759,513 (GRCm39)	missense	possibly damaging	0.93
R1792:Trrap	UTSW	5	144,790,396 (GRCm39)	missense	possibly damaging	0.87
R1859:Trrap	UTSW	5	144,767,761 (GRCm39)	missense	probably benign	0.04
R1861:Trrap	UTSW	5	144,752,727 (GRCm39)	splice site	probably null
R1902:Trrap	UTSW	5	144,752,863 (GRCm39)	missense	probably damaging	1.00
R1903:Trrap	UTSW	5	144,752,863 (GRCm39)	missense	probably damaging	1.00
R2021:Trrap	UTSW	5	144,790,298 (GRCm39)	missense	possibly damaging	0.94
R2026:Trrap	UTSW	5	144,739,854 (GRCm39)	missense	possibly damaging	0.86
R2036:Trrap	UTSW	5	144,765,372 (GRCm39)	missense	probably benign	0.08
R2099:Trrap	UTSW	5	144,719,049 (GRCm39)	missense	possibly damaging	0.46
R2108:Trrap	UTSW	5	144,762,684 (GRCm39)	missense	probably benign	0.01
R2174:Trrap	UTSW	5	144,758,665 (GRCm39)	missense	probably benign	0.40
R2442:Trrap	UTSW	5	144,754,776 (GRCm39)	missense	probably damaging	1.00
R2568:Trrap	UTSW	5	144,780,179 (GRCm39)	critical splice donor site	probably null
R3442:Trrap	UTSW	5	144,729,062 (GRCm39)	missense	probably benign	0.03
R3853:Trrap	UTSW	5	144,728,975 (GRCm39)	missense	probably damaging	1.00
R4401:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R4493:Trrap	UTSW	5	144,767,858 (GRCm39)	missense	probably benign	0.21
R4524:Trrap	UTSW	5	144,762,131 (GRCm39)	missense	probably benign	0.38
R4569:Trrap	UTSW	5	144,728,928 (GRCm39)	missense	probably benign	0.13
R4672:Trrap	UTSW	5	144,722,290 (GRCm39)	missense	probably damaging	0.97
R4732:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4733:Trrap	UTSW	5	144,753,380 (GRCm39)	missense	probably damaging	1.00
R4791:Trrap	UTSW	5	144,740,087 (GRCm39)	missense	probably damaging	1.00
R4795:Trrap	UTSW	5	144,769,298 (GRCm39)	missense	probably benign	0.06
R4827:Trrap	UTSW	5	144,737,758 (GRCm39)	missense	probably benign	0.02
R4839:Trrap	UTSW	5	144,782,402 (GRCm39)	missense	probably damaging	1.00
R4915:Trrap	UTSW	5	144,742,545 (GRCm39)	missense	probably damaging	0.99
R4951:Trrap	UTSW	5	144,742,530 (GRCm39)	missense	possibly damaging	0.65
R4959:Trrap	UTSW	5	144,793,770 (GRCm39)	missense	probably damaging	1.00
R5049:Trrap	UTSW	5	144,763,527 (GRCm39)	missense	probably damaging	1.00
R5074:Trrap	UTSW	5	144,787,989 (GRCm39)	missense	probably damaging	1.00
R5236:Trrap	UTSW	5	144,754,596 (GRCm39)	missense	probably benign	0.07
R5281:Trrap	UTSW	5	144,750,313 (GRCm39)	missense	probably benign	0.13
R5322:Trrap	UTSW	5	144,781,034 (GRCm39)	missense	probably damaging	1.00
R5457:Trrap	UTSW	5	144,786,787 (GRCm39)	missense	probably damaging	1.00
R5590:Trrap	UTSW	5	144,719,075 (GRCm39)	missense	probably benign	0.05
R5799:Trrap	UTSW	5	144,767,755 (GRCm39)	missense	probably benign
R5885:Trrap	UTSW	5	144,731,603 (GRCm39)	missense	probably damaging	1.00
R5905:Trrap	UTSW	5	144,786,730 (GRCm39)	missense	possibly damaging	0.95
R5908:Trrap	UTSW	5	144,723,518 (GRCm39)	missense	probably damaging	0.96
R5956:Trrap	UTSW	5	144,744,201 (GRCm39)	splice site	silent
R5992:Trrap	UTSW	5	144,746,994 (GRCm39)	missense	probably benign	0.00
R6017:Trrap	UTSW	5	144,781,051 (GRCm39)	missense	probably damaging	1.00
R6029:Trrap	UTSW	5	144,762,724 (GRCm39)	missense	possibly damaging	0.75
R6029:Trrap	UTSW	5	144,754,489 (GRCm39)	missense	possibly damaging	0.94
R6117:Trrap	UTSW	5	144,739,771 (GRCm39)	missense	possibly damaging	0.78
R6166:Trrap	UTSW	5	144,718,791 (GRCm39)	missense	possibly damaging	0.66
R6234:Trrap	UTSW	5	144,776,523 (GRCm39)	splice site	probably null
R6288:Trrap	UTSW	5	144,748,802 (GRCm39)	missense	probably damaging	1.00
R6290:Trrap	UTSW	5	144,741,828 (GRCm39)	missense	probably damaging	1.00
R6316:Trrap	UTSW	5	144,750,336 (GRCm39)	missense	probably benign	0.02
R6398:Trrap	UTSW	5	144,727,680 (GRCm39)	missense	possibly damaging	0.83
R6413:Trrap	UTSW	5	144,720,856 (GRCm39)	missense	possibly damaging	0.83
R6499:Trrap	UTSW	5	144,793,812 (GRCm39)	missense	probably damaging	1.00
R6529:Trrap	UTSW	5	144,771,014 (GRCm39)	missense	probably benign	0.06
R6574:Trrap	UTSW	5	144,752,360 (GRCm39)	critical splice donor site	probably null
R6631:Trrap	UTSW	5	144,708,460 (GRCm39)	missense	possibly damaging	0.94
R6727:Trrap	UTSW	5	144,793,760 (GRCm39)	missense	probably damaging	1.00
R6776:Trrap	UTSW	5	144,788,066 (GRCm39)	nonsense	probably null
R6914:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6942:Trrap	UTSW	5	144,720,853 (GRCm39)	missense	possibly damaging	0.83
R6945:Trrap	UTSW	5	144,727,665 (GRCm39)	missense	possibly damaging	0.66
R7023:Trrap	UTSW	5	144,728,964 (GRCm39)	missense	possibly damaging	0.64
R7107:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7139:Trrap	UTSW	5	144,739,988 (GRCm39)	missense	possibly damaging	0.65
R7148:Trrap	UTSW	5	144,758,613 (GRCm39)	missense	possibly damaging	0.77
R7167:Trrap	UTSW	5	144,776,424 (GRCm39)	missense	probably benign	0.39
R7171:Trrap	UTSW	5	144,730,859 (GRCm39)	missense	probably damaging	1.00
R7205:Trrap	UTSW	5	144,779,517 (GRCm39)	missense	possibly damaging	0.94
R7215:Trrap	UTSW	5	144,733,945 (GRCm39)	missense	probably benign	0.05
R7255:Trrap	UTSW	5	144,795,764 (GRCm39)	missense	probably damaging	1.00
R7261:Trrap	UTSW	5	144,782,287 (GRCm39)	missense	possibly damaging	0.67
R7264:Trrap	UTSW	5	144,751,333 (GRCm39)	missense	probably benign	0.05
R7372:Trrap	UTSW	5	144,726,208 (GRCm39)	missense	probably benign
R7447:Trrap	UTSW	5	144,776,284 (GRCm39)	missense	probably damaging	0.97
R7449:Trrap	UTSW	5	144,788,019 (GRCm39)	missense	probably damaging	1.00
R7655:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7656:Trrap	UTSW	5	144,779,422 (GRCm39)	missense	probably damaging	1.00
R7662:Trrap	UTSW	5	144,769,321 (GRCm39)	missense	probably benign	0.00
R7716:Trrap	UTSW	5	144,713,956 (GRCm39)	missense	possibly damaging	0.73
R8143:Trrap	UTSW	5	144,772,707 (GRCm39)	splice site	probably null
R8183:Trrap	UTSW	5	144,765,343 (GRCm39)	missense	probably benign	0.01
R8265:Trrap	UTSW	5	144,722,344 (GRCm39)	missense	possibly damaging	0.53
R8273:Trrap	UTSW	5	144,727,975 (GRCm39)	missense	probably damaging	1.00
R8556:Trrap	UTSW	5	144,762,747 (GRCm39)	missense	probably benign	0.44
R8674:Trrap	UTSW	5	144,727,842 (GRCm39)	missense	probably benign	0.02
R8777:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8777-TAIL:Trrap	UTSW	5	144,773,949 (GRCm39)	missense	probably benign	0.10
R8817:Trrap	UTSW	5	144,782,348 (GRCm39)	missense	probably damaging	1.00
R8841:Trrap	UTSW	5	144,781,021 (GRCm39)	missense	probably damaging	1.00
R8871:Trrap	UTSW	5	144,758,649 (GRCm39)	missense	probably benign	0.30
R8937:Trrap	UTSW	5	144,757,063 (GRCm39)	missense	probably damaging	1.00
R8966:Trrap	UTSW	5	144,740,162 (GRCm39)	missense	probably damaging	0.96
R9010:Trrap	UTSW	5	144,783,226 (GRCm39)	missense	probably damaging	1.00
R9095:Trrap	UTSW	5	144,733,961 (GRCm39)	missense	probably damaging	1.00
R9127:Trrap	UTSW	5	144,767,830 (GRCm39)	missense	probably benign	0.16
R9132:Trrap	UTSW	5	144,726,362 (GRCm39)	missense	probably benign	0.03
R9224:Trrap	UTSW	5	144,708,049 (GRCm39)	missense	possibly damaging	0.70
R9338:Trrap	UTSW	5	144,727,925 (GRCm39)	missense	probably benign
R9380:Trrap	UTSW	5	144,769,981 (GRCm39)	missense	probably benign
R9404:Trrap	UTSW	5	144,752,225 (GRCm39)	missense	possibly damaging	0.85
R9457:Trrap	UTSW	5	144,763,478 (GRCm39)	missense	probably damaging	1.00
R9464:Trrap	UTSW	5	144,763,517 (GRCm39)	missense	probably damaging	0.99
R9504:Trrap	UTSW	5	144,742,904 (GRCm39)	missense	probably damaging	1.00
R9583:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9584:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9585:Trrap	UTSW	5	144,777,330 (GRCm39)	missense	probably damaging	1.00
R9608:Trrap	UTSW	5	144,780,128 (GRCm39)	missense	possibly damaging	0.60
R9728:Trrap	UTSW	5	144,726,193 (GRCm39)	missense	probably benign	0.22
R9782:Trrap	UTSW	5	144,758,716 (GRCm39)	missense	probably damaging	0.99
X0060:Trrap	UTSW	5	144,780,171 (GRCm39)	missense	probably damaging	0.96
Z1088:Trrap	UTSW	5	144,771,007 (GRCm39)	missense	probably benign	0.00
Z1177:Trrap	UTSW	5	144,756,518 (GRCm39)	missense	probably damaging	1.00
Z1177:Trrap	UTSW	5	144,747,154 (GRCm39)	missense
Z1177:Trrap	UTSW	5	144,793,761 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TATTCCATCTGTTGGTCAGCAAC -3'
(R):5'- AGCCTGACTACATGTGGGAG -3'

Sequencing Primer
(F):5'- TTGACCCAAGTGCACAAC -3'
(R):5'- CAGGGGTGTGCCTATCAC -3'

Posted On

2014-09-18