Mutagenetix > Incidental Mutation

Incidental Mutation 'R2145:Rif1'

233699

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

040148-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2145 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 52111400 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 1622 (I1622N)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000069794
AA Change: I1622N

PolyPhen 2 Score 0.625 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: I1622N

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112693
AA Change: I1622N

PolyPhen 2 Score 0.625 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: I1622N

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000125322
AA Change: I139N

Predicted Effect

probably benign
Transcript: ENSMUST00000125376

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.6%

Validation Efficiency

97% (101/104)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032A03Rik	T	C	9: 50,764,874 (GRCm38)	Y32C	probably damaging	Het
Abca15	A	G	7: 120,354,478 (GRCm38)	N535S	probably benign	Het
Abcc6	A	T	7: 45,998,741 (GRCm38)	L717Q	probably benign	Het
Abraxas2	C	A	7: 132,883,061 (GRCm38)	Q278K	probably benign	Het
Acap2	A	T	16: 31,105,524 (GRCm38)	D637E	probably benign	Het
AI661453	A	G	17: 47,466,098 (GRCm38)		probably benign	Het
Aoah	A	T	13: 20,840,096 (GRCm38)	E74V	probably damaging	Het
Appl1	A	G	14: 26,949,619 (GRCm38)	L292S	possibly damaging	Het
Astl	T	A	2: 127,347,189 (GRCm38)	V166E	probably damaging	Het
Atp5s	A	G	12: 69,741,054 (GRCm38)	Q88R	probably damaging	Het
Bbs1	T	G	19: 4,903,707 (GRCm38)	K143Q	possibly damaging	Het
Bbx	T	C	16: 50,274,544 (GRCm38)		probably benign	Het
Birc6	A	T	17: 74,660,413 (GRCm38)	Q4103L	possibly damaging	Het
C1qtnf2	T	G	11: 43,490,984 (GRCm38)	F178V	probably damaging	Het
Camta2	A	G	11: 70,671,575 (GRCm38)	F999L	probably benign	Het
Clcn7	A	G	17: 25,144,451 (GRCm38)	I34V	probably benign	Het
Cntn5	G	T	9: 9,748,415 (GRCm38)	P487Q	probably damaging	Het
Ctu2	T	A	8: 122,479,152 (GRCm38)	I213K	probably benign	Het
Des	C	A	1: 75,363,464 (GRCm38)		probably benign	Het
Dgcr8	A	T	16: 18,280,230 (GRCm38)	D432E	probably benign	Het
Dlgap5	G	A	14: 47,395,923 (GRCm38)	R549*	probably null	Het
Dmxl2	T	C	9: 54,415,910 (GRCm38)	T1397A	probably damaging	Het
Dnmt1	T	A	9: 20,937,155 (GRCm38)		probably benign	Het
Doxl2	A	T	6: 48,976,695 (GRCm38)	D518V	probably damaging	Het
Dstyk	T	A	1: 132,463,375 (GRCm38)	M838K	probably damaging	Het
Dtwd1	C	A	2: 126,159,984 (GRCm38)	T208N	probably damaging	Het
Dvl1	G	A	4: 155,847,816 (GRCm38)	V28I	possibly damaging	Het
Dync1i2	T	A	2: 71,214,563 (GRCm38)		probably benign	Het
Fer1l6	T	A	15: 58,627,534 (GRCm38)	M1251K	probably benign	Het
Fmod	T	C	1: 134,040,518 (GRCm38)	Y99H	probably benign	Het
Fn1	A	T	1: 71,606,004 (GRCm38)	V1552D	probably damaging	Het
Fnip2	A	T	3: 79,500,432 (GRCm38)	S281T	probably damaging	Het
Glb1	A	G	9: 114,464,165 (GRCm38)	H536R	probably benign	Het
Glis2	T	A	16: 4,613,642 (GRCm38)	S344R	possibly damaging	Het
Gm1966	T	A	7: 106,603,008 (GRCm38)	H343L	possibly damaging	Het
Gm4847	A	T	1: 166,634,903 (GRCm38)	S339R	probably benign	Het
Gpr155	A	G	2: 73,356,658 (GRCm38)	S44P	probably benign	Het
Gprin1	G	A	13: 54,738,632 (GRCm38)	P610S	probably damaging	Het
H2-Ob	A	T	17: 34,242,580 (GRCm38)	M98L	probably benign	Het
Hist1h3e	T	C	13: 23,562,356 (GRCm38)	T4A	probably benign	Het
Hmcn2	T	C	2: 31,333,931 (GRCm38)		probably benign	Het
Ikbke	C	A	1: 131,273,474 (GRCm38)	V176L	probably damaging	Het
Il13	T	C	11: 53,632,524 (GRCm38)	T85A	possibly damaging	Het
Inpp5k	A	T	11: 75,647,191 (GRCm38)		probably null	Het
Irgm2	T	C	11: 58,220,529 (GRCm38)	S361P	possibly damaging	Het
Itga11	C	T	9: 62,732,204 (GRCm38)		probably benign	Het
Kalrn	G	T	16: 34,009,262 (GRCm38)		probably benign	Het
Kcng1	C	A	2: 168,269,032 (GRCm38)	G71C	probably damaging	Het
Kcnq5	T	A	1: 21,505,349 (GRCm38)	D291V	probably damaging	Het
Klhl7	A	T	5: 24,100,863 (GRCm38)	M37L	probably benign	Het
Letm1	A	AG	5: 33,769,515 (GRCm38)		probably null	Het
Lhx6	C	T	2: 36,087,466 (GRCm38)	V325I	probably benign	Het
Lipc	A	G	9: 70,934,535 (GRCm38)	I9T	possibly damaging	Het
Lsmem1	GTACATACATACATACATACATACATACA	GTACATACATACATACATACATACATACATACA	12: 40,185,261 (GRCm38)		probably null	Het
Mast1	C	A	8: 84,921,478 (GRCm38)	G458V	probably damaging	Het
Mga	T	C	2: 119,964,157 (GRCm38)	V2565A	possibly damaging	Het
Mkl2	T	C	16: 13,412,586 (GRCm38)	I1045T	probably damaging	Het
Mpzl2	C	G	9: 45,044,173 (GRCm38)	D127E	probably benign	Het
Myh3	T	A	11: 67,091,056 (GRCm38)	C793S	probably benign	Het
Nomo1	T	C	7: 46,066,504 (GRCm38)	L765P	probably damaging	Het
Nup210	A	G	6: 91,028,876 (GRCm38)	I1335T	possibly damaging	Het
Olfr549	T	C	7: 102,555,060 (GRCm38)		probably null	Het
Oxr1	T	A	15: 41,819,944 (GRCm38)	S254R	probably damaging	Het
Pan3	A	G	5: 147,530,098 (GRCm38)	I592V	possibly damaging	Het
Pask	C	T	1: 93,321,297 (GRCm38)	A794T	probably benign	Het
Pex2	T	C	3: 5,561,590 (GRCm38)	E53G	probably damaging	Het
Pfkfb2	T	C	1: 130,698,723 (GRCm38)	T438A	probably benign	Het
Phactr4	T	C	4: 132,370,784 (GRCm38)	E391G	probably damaging	Het
Pira2	A	T	7: 3,844,345 (GRCm38)	L115Q	probably damaging	Het
Pkhd1l1	A	T	15: 44,512,877 (GRCm38)		probably null	Het
Pnlip	A	G	19: 58,676,444 (GRCm38)	S235G	probably benign	Het
Prkd1	A	G	12: 50,489,911 (GRCm38)	V130A	possibly damaging	Het
Ptpn13	A	G	5: 103,556,133 (GRCm38)	T1344A	probably benign	Het
Ptprc	T	C	1: 138,073,681 (GRCm38)	Y780C	probably damaging	Het
Pxn	T	A	5: 115,552,756 (GRCm38)		probably benign	Het
Rap1gap2	G	A	11: 74,425,976 (GRCm38)	T245M	probably damaging	Het
Rc3h1	G	T	1: 160,930,257 (GRCm38)	K48N	probably damaging	Het
Rfwd3	T	C	8: 111,282,613 (GRCm38)	I444V	probably benign	Het
Rictor	C	T	15: 6,765,107 (GRCm38)	R293C	probably damaging	Het
Rnf213	T	C	11: 119,415,193 (GRCm38)	V609A	probably benign	Het
Scgb1b2	G	T	7: 31,291,763 (GRCm38)		probably benign	Het
Serac1	A	T	17: 6,050,785 (GRCm38)	I448N	probably damaging	Het
Sh3kbp1	C	A	X: 159,824,496 (GRCm38)	T200K	probably benign	Het
Sned1	T	A	1: 93,271,684 (GRCm38)	F495L	probably damaging	Het
Socs7	T	C	11: 97,373,124 (GRCm38)	F281L	probably benign	Het
Spta1	C	T	1: 174,212,614 (GRCm38)	L1214F	probably benign	Het
Ssu72	A	G	4: 155,705,443 (GRCm38)	E21G	probably damaging	Het
Syngr4	A	G	7: 45,887,040 (GRCm38)	V186A	probably benign	Het
Tarsl2	G	A	7: 65,655,791 (GRCm38)	M254I	possibly damaging	Het
Tmem130	C	A	5: 144,743,785 (GRCm38)	V270L	probably benign	Het
Trim66	A	T	7: 109,475,113 (GRCm38)	I647N	probably damaging	Het
Tspyl2	A	T	X: 152,338,894 (GRCm38)	D572E	probably benign	Het
Unc45b	G	A	11: 82,917,754 (GRCm38)	R222H	probably benign	Het
Uxs1	T	C	1: 43,827,623 (GRCm38)	Y29C	probably damaging	Het
Virma	T	A	4: 11,548,726 (GRCm38)		probably benign	Het
Vmn1r202	C	T	13: 22,501,783 (GRCm38)	G155S	possibly damaging	Het
Vmn2r24	T	A	6: 123,779,013 (GRCm38)	F15I	probably benign	Het
Wdr64	A	G	1: 175,767,095 (GRCm38)	T471A	probably benign	Het
Zfa-ps	T	A	10: 52,543,277 (GRCm38)		noncoding transcript	Het
Zfp260	A	G	7: 30,105,340 (GRCm38)	K222E	probably damaging	Het
Zfp300	A	G	X: 21,081,951 (GRCm38)	S525P	possibly damaging	Het
Zfp821	A	G	8: 109,724,347 (GRCm38)	D324G	probably damaging	Het
Zfp934	T	C	13: 62,517,834 (GRCm38)	D331G	probably damaging	Het
Zscan29	T	C	2: 121,170,106 (GRCm38)	R7G	probably damaging	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2125:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52,098,471 (GRCm38)	nonsense	probably null
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52,084,986 (GRCm38)	splice site	probably null
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52,111,824 (GRCm38)	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52,111,139 (GRCm38)	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTTCTGAGGCAAAGGAAGAAGTTTC -3'
(R):5'- AGAGCTGTCTGATACCCTCAC -3'

Sequencing Primer
(F):5'- TCTAGAAAGAAACGATCAGGGAAATG -3'
(R):5'- AGAGCTGTCTGATACCCTCACATTAC -3'

Posted On

2014-10-01