Mutagenetix > Incidental Mutation

Incidental Mutation 'R2147:Mycbp2'

233951

Institutional Source

Beutler Lab

Gene Symbol

Mycbp2

Ensembl Gene

ENSMUSG00000033004

Gene Name

MYC binding protein 2, E3 ubiquitin protein ligase

Synonyms

Phr1, Pam, C130061D10Rik

MMRRC Submission

040150-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2147 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

103350847-103584250 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 103393358 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Tyrosine at position 3068 (H3068Y)

Ref Sequence

ENSEMBL: ENSMUSP00000124710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159855] [ENSMUST00000160758]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000159855
AA Change: H3068Y

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000124710
Gene: ENSMUSG00000033004
AA Change: H3068Y

Domain	Start	End	E-Value	Type
low complexity region	5	27	N/A	INTRINSIC
low complexity region	47	55	N/A	INTRINSIC
low complexity region	100	127	N/A	INTRINSIC
low complexity region	178	191	N/A	INTRINSIC
Pfam:RCC1_2	683	712	1.4e-10	PFAM
low complexity region	737	750	N/A	INTRINSIC
low complexity region	793	815	N/A	INTRINSIC
Pfam:RCC1_2	942	971	5.5e-10	PFAM
Pfam:RCC1	958	1006	4.8e-15	PFAM
Pfam:PHR	1235	1385	8.2e-44	PFAM
Pfam:PHR	1723	1880	1.4e-43	PFAM
low complexity region	1935	1948	N/A	INTRINSIC
low complexity region	2182	2195	N/A	INTRINSIC
Pfam:Filamin	2261	2431	7.5e-9	PFAM
Pfam:SH3_3	2472	2539	4.1e-9	PFAM
internal_repeat_3	2612	2679	1.69e-7	PROSPERO
low complexity region	2701	2710	N/A	INTRINSIC
low complexity region	2884	2917	N/A	INTRINSIC
low complexity region	2970	2984	N/A	INTRINSIC
coiled coil region	3263	3290	N/A	INTRINSIC
low complexity region	3352	3365	N/A	INTRINSIC
low complexity region	3418	3433	N/A	INTRINSIC
low complexity region	3678	3695	N/A	INTRINSIC
APC10	3810	3968	1.11e-18	SMART
low complexity region	4103	4115	N/A	INTRINSIC
low complexity region	4190	4212	N/A	INTRINSIC
Blast:BBOX	4327	4370	7e-7	BLAST
RING	4496	4546	5.35e-5	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160758
AA Change: H2958Y

PolyPhen 2 Score 0.951 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124601
Gene: ENSMUSG00000033004
AA Change: H2958Y

Domain	Start	End	E-Value	Type
low complexity region	14	22	N/A	INTRINSIC
low complexity region	67	94	N/A	INTRINSIC
low complexity region	145	158	N/A	INTRINSIC
Pfam:RCC1_2	650	679	1e-10	PFAM
low complexity region	704	717	N/A	INTRINSIC
low complexity region	760	782	N/A	INTRINSIC
Pfam:RCC1_2	909	938	1.5e-9	PFAM
Pfam:RCC1	925	973	1.3e-15	PFAM
Pfam:PHR	1202	1353	1.6e-50	PFAM
Pfam:PHR	1690	1848	3.1e-58	PFAM
low complexity region	1902	1915	N/A	INTRINSIC
low complexity region	2149	2162	N/A	INTRINSIC
Pfam:Filamin	2228	2398	7.6e-9	PFAM
Pfam:SH3_3	2439	2507	2.3e-10	PFAM
internal_repeat_3	2554	2621	2e-7	PROSPERO
low complexity region	2643	2652	N/A	INTRINSIC
low complexity region	2774	2807	N/A	INTRINSIC
low complexity region	2860	2874	N/A	INTRINSIC
coiled coil region	3153	3180	N/A	INTRINSIC
low complexity region	3242	3255	N/A	INTRINSIC
low complexity region	3308	3323	N/A	INTRINSIC
low complexity region	3568	3585	N/A	INTRINSIC
APC10	3700	3858	1.11e-18	SMART
low complexity region	3993	4005	N/A	INTRINSIC
low complexity region	4080	4102	N/A	INTRINSIC
Blast:BBOX	4217	4260	7e-7	BLAST
RING	4386	4436	5.35e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000161008

SMART Domains

Protein: ENSMUSP00000124443
Gene: ENSMUSG00000033004

Domain	Start	End	E-Value	Type
low complexity region	66	79	N/A	INTRINSIC
low complexity region	132	147	N/A	INTRINSIC
low complexity region	392	409	N/A	INTRINSIC
APC10	524	682	1.11e-18	SMART
low complexity region	820	832	N/A	INTRINSIC
low complexity region	907	929	N/A	INTRINSIC
Blast:BBOX	1042	1085	2e-6	BLAST
RING	1213	1263	5.35e-5	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000161278
AA Change: H668Y

SMART Domains

Protein: ENSMUSP00000125189
Gene: ENSMUSG00000033004
AA Change: H668Y

Domain	Start	End	E-Value	Type
Blast:IG_FLMN	2	66	1e-37	BLAST
SCOP:d1qfha1	14	64	2e-7	SMART
Pfam:SH3_3	98	165	5.8e-10	PFAM
internal_repeat_1	213	280	8.77e-8	PROSPERO
low complexity region	302	311	N/A	INTRINSIC
low complexity region	485	518	N/A	INTRINSIC
low complexity region	571	585	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162762

Meta Mutation Damage Score

0.1075

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a targeted allele exhibit neonatal lethality, defective diaphragm innervation, abnormal brain morphology and defective axonal guidance. [provided by MGI curators]

Allele List at MGI

All alleles(10) : Targeted, knock-out(1) Targeted, other(1) Gene trapped(5) Chemically induced(3)

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930523C07Rik	T	A	1: 159,903,003 (GRCm39)	M91K	probably benign	Het
Abr	T	C	11: 76,346,474 (GRCm39)	R437G	probably damaging	Het
Acaca	T	G	11: 84,167,362 (GRCm39)	D1045E	probably benign	Het
Adam23	A	T	1: 63,573,521 (GRCm39)		probably null	Het
Adam34	A	G	8: 44,105,538 (GRCm39)	Y36H	probably benign	Het
Alg11	G	A	8: 22,555,309 (GRCm39)	G108D	probably damaging	Het
Alox12e	A	T	11: 70,210,771 (GRCm39)	I316N	probably damaging	Het
Arid1a	A	T	4: 133,408,677 (GRCm39)	F1943L	unknown	Het
Armh4	T	C	14: 49,989,028 (GRCm39)	D647G	probably benign	Het
Auh	G	A	13: 52,989,532 (GRCm39)	P308L	probably benign	Het
BC035044	T	C	6: 128,867,867 (GRCm39)		probably benign	Het
Bcor	G	A	X: 11,923,862 (GRCm39)	A578V	possibly damaging	Het
C1qtnf12	A	G	4: 156,050,922 (GRCm39)	N297S	probably benign	Het
Cadps2	G	T	6: 23,838,998 (GRCm39)		probably benign	Het
Ccnf	A	T	17: 24,449,288 (GRCm39)		probably null	Het
Cdadc1	A	G	14: 59,835,202 (GRCm39)		probably null	Het
Cep57l1	A	G	10: 41,616,895 (GRCm39)	Y131H	probably damaging	Het
Cfap251	T	C	5: 123,394,254 (GRCm39)	V381A	probably benign	Het
Cfap44	A	G	16: 44,272,047 (GRCm39)	R1267G	probably benign	Het
Chd3	A	T	11: 69,239,854 (GRCm39)	L1658Q	probably benign	Het
Chl1	T	C	6: 103,692,362 (GRCm39)		probably null	Het
Chpf2	T	A	5: 24,797,033 (GRCm39)	F660I	probably damaging	Het
Cmas	T	A	6: 142,717,015 (GRCm39)	D302E	probably benign	Het
Cpne3	T	A	4: 19,536,562 (GRCm39)	M233L	probably benign	Het
Csmd3	CCTTTGCGCTT	CCTT	15: 47,604,632 (GRCm39)		probably null	Het
Dennd3	T	G	15: 73,395,336 (GRCm39)	L143R	probably damaging	Het
Dnah2	T	C	11: 69,406,587 (GRCm39)	M552V	probably benign	Het
Dock2	A	G	11: 34,179,472 (GRCm39)		probably null	Het
Ergic1	A	G	17: 26,855,024 (GRCm39)		probably null	Het
Fbxl15	A	T	19: 46,317,627 (GRCm39)	D103V	probably damaging	Het
Gm4781	C	A	10: 100,232,414 (GRCm39)		noncoding transcript	Het
Hpca	A	G	4: 129,012,278 (GRCm39)	I86T	possibly damaging	Het
Katnip	T	A	7: 125,464,492 (GRCm39)	H1286Q	probably damaging	Het
Lamb3	T	C	1: 193,010,212 (GRCm39)	V275A	probably benign	Het
Lipe	G	T	7: 25,087,946 (GRCm39)	A38E	probably benign	Het
Lrrk1	A	T	7: 65,935,159 (GRCm39)		probably null	Het
Lrsam1	T	C	2: 32,835,891 (GRCm39)	K292R	probably damaging	Het
Mbtps1	A	T	8: 120,265,598 (GRCm39)	H316Q	probably benign	Het
Mms22l	T	A	4: 24,580,063 (GRCm39)	Y525*	probably null	Het
Mrps14	T	C	1: 160,022,862 (GRCm39)	L9P	possibly damaging	Het
Myo15a	A	G	11: 60,401,055 (GRCm39)	D2992G	possibly damaging	Het
N4bp2	T	A	5: 65,966,543 (GRCm39)	L1327Q	probably damaging	Het
Nradd	A	T	9: 110,451,243 (GRCm39)	F42I	probably benign	Het
Or5k8	T	A	16: 58,644,842 (GRCm39)	I77F	probably damaging	Het
Pank1	T	C	19: 34,804,754 (GRCm39)	H134R	probably benign	Het
Pcdh7	T	A	5: 58,286,458 (GRCm39)	M1178K	possibly damaging	Het
Pcnx3	A	T	19: 5,717,633 (GRCm39)	I1084N	probably damaging	Het
Pdcd11	A	G	19: 47,093,191 (GRCm39)	M490V	probably benign	Het
Phf2	T	C	13: 48,958,165 (GRCm39)	K950E	unknown	Het
Pitpnc1	C	T	11: 107,103,344 (GRCm39)	A252T	probably damaging	Het
Prickle2	A	G	6: 92,402,652 (GRCm39)	L112P	probably damaging	Het
Prpf8	A	G	11: 75,381,357 (GRCm39)	I231V	probably benign	Het
Scin	T	A	12: 40,130,984 (GRCm39)	M310L	probably benign	Het
Serpina3m	T	A	12: 104,355,483 (GRCm39)	I50N	probably benign	Het
Skint8	C	G	4: 111,794,274 (GRCm39)	N221K	probably damaging	Het
Slc22a23	C	T	13: 34,366,990 (GRCm39)	V673M	probably benign	Het
Slc24a5	A	G	2: 124,929,361 (GRCm39)	D368G	probably damaging	Het
Slc28a1	A	T	7: 80,776,015 (GRCm39)	Q237L	possibly damaging	Het
Smchd1	T	A	17: 71,705,583 (GRCm39)	K1005N	possibly damaging	Het
Stim2	T	C	5: 54,262,717 (GRCm39)	Y320H	probably damaging	Het
Syne3	T	A	12: 104,919,357 (GRCm39)	D512V	probably damaging	Het
Tada2a	T	C	11: 83,970,455 (GRCm39)	D432G	probably damaging	Het
Tcp10a	T	C	17: 7,601,701 (GRCm39)	S216P	probably damaging	Het
Tmem178b	A	T	6: 40,184,435 (GRCm39)	Q111L	probably damaging	Het
Tmem236	A	G	2: 14,223,861 (GRCm39)	I217V	probably benign	Het
Tmem45b	A	G	9: 31,340,277 (GRCm39)	V128A	probably benign	Het
Tnfaip2	T	C	12: 111,412,456 (GRCm39)	Y286H	probably damaging	Het
Tsc2	T	A	17: 24,840,116 (GRCm39)	I427L	possibly damaging	Het
Ttc17	T	A	2: 94,132,139 (GRCm39)	N1180I	possibly damaging	Het
Ubr1	T	G	2: 120,694,811 (GRCm39)	D1707A	probably damaging	Het
Vmn1r11	A	G	6: 57,114,583 (GRCm39)	I82M	probably benign	Het
Vmn2r129	T	C	4: 156,687,014 (GRCm39)		noncoding transcript	Het
Vps41	G	T	13: 19,023,904 (GRCm39)		probably null	Het
Wnt5a	A	G	14: 28,235,274 (GRCm39)	Y86C	probably damaging	Het
Zfp629	T	C	7: 127,209,616 (GRCm39)	H731R	probably damaging	Het
Zfp712	T	C	13: 67,189,960 (GRCm39)	E189G	possibly damaging	Het

Other mutations in Mycbp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Mycbp2	APN	14	103,460,486 (GRCm39)	missense	probably damaging	1.00
IGL00518:Mycbp2	APN	14	103,393,244 (GRCm39)	missense	probably damaging	1.00
IGL00650:Mycbp2	APN	14	103,380,664 (GRCm39)	missense	probably damaging	0.97
IGL00653:Mycbp2	APN	14	103,380,664 (GRCm39)	missense	probably damaging	0.97
IGL00742:Mycbp2	APN	14	103,438,788 (GRCm39)	missense	probably damaging	1.00
IGL00755:Mycbp2	APN	14	103,432,057 (GRCm39)	missense	possibly damaging	0.72
IGL00793:Mycbp2	APN	14	103,364,189 (GRCm39)	missense	possibly damaging	0.77
IGL00916:Mycbp2	APN	14	103,528,719 (GRCm39)	splice site	probably benign
IGL00960:Mycbp2	APN	14	103,466,820 (GRCm39)	missense	possibly damaging	0.95
IGL00977:Mycbp2	APN	14	103,410,078 (GRCm39)	missense	probably damaging	0.98
IGL01349:Mycbp2	APN	14	103,359,983 (GRCm39)	missense	probably damaging	0.98
IGL01369:Mycbp2	APN	14	103,392,946 (GRCm39)	missense	possibly damaging	0.61
IGL01410:Mycbp2	APN	14	103,466,928 (GRCm39)	splice site	probably null
IGL01586:Mycbp2	APN	14	103,378,305 (GRCm39)	critical splice donor site	probably null
IGL01593:Mycbp2	APN	14	103,528,723 (GRCm39)	critical splice donor site	probably null
IGL01693:Mycbp2	APN	14	103,365,415 (GRCm39)	missense	probably damaging	0.99
IGL01730:Mycbp2	APN	14	103,372,640 (GRCm39)	nonsense	probably null
IGL01820:Mycbp2	APN	14	103,425,937 (GRCm39)	missense	probably damaging	1.00
IGL01974:Mycbp2	APN	14	103,380,647 (GRCm39)	missense	possibly damaging	0.88
IGL02071:Mycbp2	APN	14	103,392,343 (GRCm39)	nonsense	probably null
IGL02178:Mycbp2	APN	14	103,461,802 (GRCm39)	missense	probably benign	0.01
IGL02324:Mycbp2	APN	14	103,479,643 (GRCm39)	missense	probably damaging	1.00
IGL02442:Mycbp2	APN	14	103,551,811 (GRCm39)	missense	probably benign
IGL02607:Mycbp2	APN	14	103,522,709 (GRCm39)	missense	probably damaging	1.00
IGL02679:Mycbp2	APN	14	103,442,621 (GRCm39)	missense	probably benign
IGL02702:Mycbp2	APN	14	103,457,560 (GRCm39)	missense	probably benign	0.01
IGL02709:Mycbp2	APN	14	103,392,697 (GRCm39)	missense	probably damaging	0.97
IGL02736:Mycbp2	APN	14	103,351,678 (GRCm39)	splice site	probably benign
IGL02866:Mycbp2	APN	14	103,367,428 (GRCm39)	missense	probably damaging	0.98
IGL02939:Mycbp2	APN	14	103,414,715 (GRCm39)	missense	probably benign
IGL03082:Mycbp2	APN	14	103,441,805 (GRCm39)	missense	probably benign	0.23
IGL03142:Mycbp2	APN	14	103,536,212 (GRCm39)	missense	probably damaging	0.99
IGL03155:Mycbp2	APN	14	103,392,889 (GRCm39)	missense	probably benign	0.06
IGL03236:Mycbp2	APN	14	103,536,134 (GRCm39)	missense	probably damaging	0.99
IGL03256:Mycbp2	APN	14	103,426,025 (GRCm39)	missense	possibly damaging	0.92
IGL03303:Mycbp2	APN	14	103,485,194 (GRCm39)	missense	probably damaging	1.00
compost	UTSW	14	103,393,889 (GRCm39)	missense	probably damaging	1.00
decompose	UTSW	14	103,457,415 (GRCm39)	missense	probably benign	0.12
moulder	UTSW	14	103,426,028 (GRCm39)	missense	probably damaging	1.00
N/A - 293:Mycbp2	UTSW	14	103,461,898 (GRCm39)	splice site	probably benign
R0040:Mycbp2	UTSW	14	103,461,708 (GRCm39)	missense	probably benign	0.11
R0040:Mycbp2	UTSW	14	103,461,708 (GRCm39)	missense	probably benign	0.11
R0057:Mycbp2	UTSW	14	103,389,578 (GRCm39)	missense	probably damaging	0.97
R0063:Mycbp2	UTSW	14	103,394,070 (GRCm39)	unclassified	probably benign
R0097:Mycbp2	UTSW	14	103,393,198 (GRCm39)	missense	probably damaging	1.00
R0097:Mycbp2	UTSW	14	103,393,198 (GRCm39)	missense	probably damaging	1.00
R0268:Mycbp2	UTSW	14	103,551,761 (GRCm39)	nonsense	probably null
R0388:Mycbp2	UTSW	14	103,394,103 (GRCm39)	missense	probably benign	0.01
R0410:Mycbp2	UTSW	14	103,372,569 (GRCm39)	missense	probably damaging	1.00
R0530:Mycbp2	UTSW	14	103,419,895 (GRCm39)	missense	probably damaging	1.00
R0591:Mycbp2	UTSW	14	103,433,827 (GRCm39)	unclassified	probably benign
R0671:Mycbp2	UTSW	14	103,432,024 (GRCm39)	missense	possibly damaging	0.95
R0755:Mycbp2	UTSW	14	103,412,230 (GRCm39)	missense	probably damaging	1.00
R0817:Mycbp2	UTSW	14	103,466,854 (GRCm39)	missense	probably damaging	0.99
R0818:Mycbp2	UTSW	14	103,466,854 (GRCm39)	missense	probably damaging	0.99
R0819:Mycbp2	UTSW	14	103,466,854 (GRCm39)	missense	probably damaging	0.99
R0881:Mycbp2	UTSW	14	103,457,449 (GRCm39)	missense	probably benign
R0903:Mycbp2	UTSW	14	103,513,293 (GRCm39)	missense	probably damaging	0.99
R0940:Mycbp2	UTSW	14	103,500,129 (GRCm39)	unclassified	probably benign
R0961:Mycbp2	UTSW	14	103,422,271 (GRCm39)	missense	probably damaging	1.00
R1004:Mycbp2	UTSW	14	103,378,353 (GRCm39)	missense	probably benign	0.00
R1138:Mycbp2	UTSW	14	103,412,262 (GRCm39)	missense	possibly damaging	0.84
R1170:Mycbp2	UTSW	14	103,437,588 (GRCm39)	nonsense	probably null
R1211:Mycbp2	UTSW	14	103,357,999 (GRCm39)	missense	probably benign	0.31
R1268:Mycbp2	UTSW	14	103,446,218 (GRCm39)	missense	probably damaging	1.00
R1298:Mycbp2	UTSW	14	103,393,334 (GRCm39)	missense	probably damaging	1.00
R1341:Mycbp2	UTSW	14	103,536,303 (GRCm39)	splice site	probably benign
R1469:Mycbp2	UTSW	14	103,425,956 (GRCm39)	missense	probably damaging	0.99
R1469:Mycbp2	UTSW	14	103,425,956 (GRCm39)	missense	probably damaging	0.99
R1513:Mycbp2	UTSW	14	103,441,825 (GRCm39)	missense	probably damaging	1.00
R1528:Mycbp2	UTSW	14	103,470,033 (GRCm39)	missense	possibly damaging	0.91
R1564:Mycbp2	UTSW	14	103,407,287 (GRCm39)	splice site	probably null
R1565:Mycbp2	UTSW	14	103,489,945 (GRCm39)	missense	possibly damaging	0.82
R1656:Mycbp2	UTSW	14	103,485,194 (GRCm39)	missense	probably damaging	1.00
R1694:Mycbp2	UTSW	14	103,464,947 (GRCm39)	missense	probably damaging	1.00
R1709:Mycbp2	UTSW	14	103,461,852 (GRCm39)	missense	probably damaging	1.00
R1728:Mycbp2	UTSW	14	103,392,614 (GRCm39)	missense	probably damaging	0.98
R1751:Mycbp2	UTSW	14	103,485,841 (GRCm39)	missense	probably damaging	0.98
R1767:Mycbp2	UTSW	14	103,485,841 (GRCm39)	missense	probably damaging	0.98
R1772:Mycbp2	UTSW	14	103,419,855 (GRCm39)	missense	probably damaging	1.00
R1784:Mycbp2	UTSW	14	103,392,614 (GRCm39)	missense	probably damaging	0.98
R1823:Mycbp2	UTSW	14	103,489,945 (GRCm39)	missense	possibly damaging	0.82
R1824:Mycbp2	UTSW	14	103,489,945 (GRCm39)	missense	possibly damaging	0.82
R1844:Mycbp2	UTSW	14	103,393,150 (GRCm39)	missense	possibly damaging	0.94
R1916:Mycbp2	UTSW	14	103,422,319 (GRCm39)	missense	probably damaging	1.00
R1944:Mycbp2	UTSW	14	103,466,840 (GRCm39)	missense	probably damaging	1.00
R1983:Mycbp2	UTSW	14	103,383,407 (GRCm39)	missense	probably damaging	0.97
R2002:Mycbp2	UTSW	14	103,485,839 (GRCm39)	missense	probably damaging	0.98
R2031:Mycbp2	UTSW	14	103,426,028 (GRCm39)	missense	probably damaging	1.00
R2035:Mycbp2	UTSW	14	103,497,675 (GRCm39)	missense	probably damaging	1.00
R2048:Mycbp2	UTSW	14	103,469,960 (GRCm39)	critical splice donor site	probably null
R2061:Mycbp2	UTSW	14	103,524,696 (GRCm39)	missense	probably damaging	0.99
R2113:Mycbp2	UTSW	14	103,457,512 (GRCm39)	missense	probably damaging	0.99
R2128:Mycbp2	UTSW	14	103,438,666 (GRCm39)	missense	probably benign	0.01
R2134:Mycbp2	UTSW	14	103,446,329 (GRCm39)	missense	probably damaging	1.00
R2135:Mycbp2	UTSW	14	103,446,329 (GRCm39)	missense	probably damaging	1.00
R2135:Mycbp2	UTSW	14	103,383,378 (GRCm39)	missense	probably benign
R2146:Mycbp2	UTSW	14	103,393,358 (GRCm39)	missense	probably damaging	0.97
R2148:Mycbp2	UTSW	14	103,393,358 (GRCm39)	missense	probably damaging	0.97
R2150:Mycbp2	UTSW	14	103,393,358 (GRCm39)	missense	probably damaging	0.97
R2163:Mycbp2	UTSW	14	103,407,291 (GRCm39)	critical splice donor site	probably null
R2248:Mycbp2	UTSW	14	103,407,295 (GRCm39)	missense	possibly damaging	0.50
R2265:Mycbp2	UTSW	14	103,500,185 (GRCm39)	missense	probably benign	0.39
R2272:Mycbp2	UTSW	14	103,381,774 (GRCm39)	missense	probably null	0.66
R2379:Mycbp2	UTSW	14	103,412,386 (GRCm39)	missense	probably benign
R2495:Mycbp2	UTSW	14	103,437,554 (GRCm39)	missense	probably damaging	0.99
R2508:Mycbp2	UTSW	14	103,368,681 (GRCm39)	missense	probably damaging	0.99
R2510:Mycbp2	UTSW	14	103,392,691 (GRCm39)	missense	probably damaging	0.96
R2851:Mycbp2	UTSW	14	103,381,769 (GRCm39)	missense	probably damaging	0.99
R2852:Mycbp2	UTSW	14	103,381,769 (GRCm39)	missense	probably damaging	0.99
R2965:Mycbp2	UTSW	14	103,534,794 (GRCm39)	missense	probably benign	0.00
R3156:Mycbp2	UTSW	14	103,446,179 (GRCm39)	splice site	probably benign
R3404:Mycbp2	UTSW	14	103,437,550 (GRCm39)	missense	probably damaging	0.99
R3410:Mycbp2	UTSW	14	103,372,553 (GRCm39)	missense	probably damaging	1.00
R3429:Mycbp2	UTSW	14	103,466,866 (GRCm39)	missense	probably damaging	1.00
R3706:Mycbp2	UTSW	14	103,393,850 (GRCm39)	missense	probably benign	0.31
R3772:Mycbp2	UTSW	14	103,371,224 (GRCm39)	missense	possibly damaging	0.82
R3778:Mycbp2	UTSW	14	103,434,721 (GRCm39)	missense	probably damaging	0.99
R3883:Mycbp2	UTSW	14	103,532,686 (GRCm39)	missense	probably damaging	0.97
R3884:Mycbp2	UTSW	14	103,532,686 (GRCm39)	missense	probably damaging	0.97
R3887:Mycbp2	UTSW	14	103,412,233 (GRCm39)	missense	probably damaging	0.98
R3923:Mycbp2	UTSW	14	103,364,149 (GRCm39)	missense	probably damaging	1.00
R3926:Mycbp2	UTSW	14	103,441,936 (GRCm39)	missense	probably damaging	1.00
R3959:Mycbp2	UTSW	14	103,532,688 (GRCm39)	missense	probably benign	0.00
R3966:Mycbp2	UTSW	14	103,376,161 (GRCm39)	splice site	probably benign
R4021:Mycbp2	UTSW	14	103,389,593 (GRCm39)	missense	probably damaging	0.97
R4363:Mycbp2	UTSW	14	103,485,893 (GRCm39)	missense	probably damaging	1.00
R4405:Mycbp2	UTSW	14	103,360,881 (GRCm39)	missense	probably damaging	1.00
R4407:Mycbp2	UTSW	14	103,524,664 (GRCm39)	missense	probably damaging	1.00
R4410:Mycbp2	UTSW	14	103,372,702 (GRCm39)	missense	probably damaging	1.00
R4434:Mycbp2	UTSW	14	103,371,225 (GRCm39)	missense	probably damaging	0.99
R4448:Mycbp2	UTSW	14	103,425,938 (GRCm39)	missense	possibly damaging	0.89
R4452:Mycbp2	UTSW	14	103,393,094 (GRCm39)	missense	probably damaging	0.99
R4573:Mycbp2	UTSW	14	103,583,733 (GRCm39)	missense	probably benign	0.05
R4589:Mycbp2	UTSW	14	103,414,749 (GRCm39)	missense	probably benign	0.04
R4621:Mycbp2	UTSW	14	103,457,415 (GRCm39)	missense	probably benign	0.12
R4622:Mycbp2	UTSW	14	103,457,415 (GRCm39)	missense	probably benign	0.12
R4729:Mycbp2	UTSW	14	103,426,027 (GRCm39)	missense	probably damaging	1.00
R4770:Mycbp2	UTSW	14	103,457,380 (GRCm39)	missense	probably benign	0.41
R4790:Mycbp2	UTSW	14	103,466,873 (GRCm39)	missense	probably damaging	1.00
R4884:Mycbp2	UTSW	14	103,448,731 (GRCm39)	missense	probably damaging	1.00
R4885:Mycbp2	UTSW	14	103,383,382 (GRCm39)	missense	possibly damaging	0.86
R4956:Mycbp2	UTSW	14	103,524,675 (GRCm39)	missense	probably damaging	0.99
R4980:Mycbp2	UTSW	14	103,497,821 (GRCm39)	splice site	probably null
R4994:Mycbp2	UTSW	14	103,407,430 (GRCm39)	missense	probably benign
R5029:Mycbp2	UTSW	14	103,393,946 (GRCm39)	missense	probably benign	0.21
R5038:Mycbp2	UTSW	14	103,534,375 (GRCm39)	missense	probably damaging	1.00
R5044:Mycbp2	UTSW	14	103,376,671 (GRCm39)	critical splice donor site	probably null
R5231:Mycbp2	UTSW	14	103,583,650 (GRCm39)	critical splice donor site	probably null
R5305:Mycbp2	UTSW	14	103,583,757 (GRCm39)	missense	probably benign	0.00
R5322:Mycbp2	UTSW	14	103,423,119 (GRCm39)	critical splice acceptor site	probably null
R5376:Mycbp2	UTSW	14	103,479,868 (GRCm39)	nonsense	probably null
R5414:Mycbp2	UTSW	14	103,543,697 (GRCm39)	missense	probably damaging	1.00
R5453:Mycbp2	UTSW	14	103,438,837 (GRCm39)	missense	probably damaging	0.99
R5462:Mycbp2	UTSW	14	103,437,562 (GRCm39)	missense	probably damaging	1.00
R5499:Mycbp2	UTSW	14	103,479,615 (GRCm39)	missense	probably damaging	1.00
R5502:Mycbp2	UTSW	14	103,411,250 (GRCm39)	missense	probably damaging	1.00
R5524:Mycbp2	UTSW	14	103,532,673 (GRCm39)	missense	probably damaging	1.00
R5533:Mycbp2	UTSW	14	103,520,081 (GRCm39)	nonsense	probably null
R5569:Mycbp2	UTSW	14	103,372,679 (GRCm39)	missense	probably damaging	1.00
R5574:Mycbp2	UTSW	14	103,380,203 (GRCm39)	missense	possibly damaging	0.94
R5579:Mycbp2	UTSW	14	103,528,769 (GRCm39)	missense	probably damaging	0.98
R5590:Mycbp2	UTSW	14	103,360,791 (GRCm39)	missense	probably damaging	1.00
R5592:Mycbp2	UTSW	14	103,432,113 (GRCm39)	missense	probably benign	0.02
R5643:Mycbp2	UTSW	14	103,524,770 (GRCm39)	missense	probably damaging	1.00
R5644:Mycbp2	UTSW	14	103,524,770 (GRCm39)	missense	probably damaging	1.00
R5645:Mycbp2	UTSW	14	103,426,051 (GRCm39)	critical splice acceptor site	probably null
R5645:Mycbp2	UTSW	14	103,426,044 (GRCm39)	missense	probably damaging	1.00
R5646:Mycbp2	UTSW	14	103,407,346 (GRCm39)	missense	probably benign	0.09
R5648:Mycbp2	UTSW	14	103,528,778 (GRCm39)	missense	probably damaging	1.00
R5651:Mycbp2	UTSW	14	103,520,101 (GRCm39)	missense	probably null	0.99
R5668:Mycbp2	UTSW	14	103,357,955 (GRCm39)	missense	possibly damaging	0.62
R5745:Mycbp2	UTSW	14	103,393,889 (GRCm39)	missense	possibly damaging	0.94
R5751:Mycbp2	UTSW	14	103,385,986 (GRCm39)	missense	probably damaging	0.99
R5756:Mycbp2	UTSW	14	103,371,410 (GRCm39)	missense	probably damaging	0.99
R5837:Mycbp2	UTSW	14	103,361,839 (GRCm39)	missense	probably damaging	1.00
R5984:Mycbp2	UTSW	14	103,364,120 (GRCm39)	missense	probably damaging	0.98
R6005:Mycbp2	UTSW	14	103,394,159 (GRCm39)	missense	probably benign
R6063:Mycbp2	UTSW	14	103,372,582 (GRCm39)	missense	probably damaging	1.00
R6091:Mycbp2	UTSW	14	103,460,482 (GRCm39)	missense	probably damaging	1.00
R6120:Mycbp2	UTSW	14	103,513,323 (GRCm39)	missense	probably benign	0.01
R6129:Mycbp2	UTSW	14	103,522,836 (GRCm39)	missense	probably benign	0.21
R6147:Mycbp2	UTSW	14	103,392,945 (GRCm39)	nonsense	probably null
R6161:Mycbp2	UTSW	14	103,536,183 (GRCm39)	missense	probably damaging	1.00
R6187:Mycbp2	UTSW	14	103,384,453 (GRCm39)	missense	probably damaging	1.00
R6208:Mycbp2	UTSW	14	103,532,664 (GRCm39)	missense	probably benign	0.11
R6228:Mycbp2	UTSW	14	103,497,665 (GRCm39)	missense	probably benign	0.24
R6301:Mycbp2	UTSW	14	103,392,862 (GRCm39)	missense	probably damaging	1.00
R6311:Mycbp2	UTSW	14	103,500,176 (GRCm39)	missense	possibly damaging	0.93
R6329:Mycbp2	UTSW	14	103,393,288 (GRCm39)	missense	probably benign	0.00
R6439:Mycbp2	UTSW	14	103,392,911 (GRCm39)	missense	probably benign	0.00
R6462:Mycbp2	UTSW	14	103,373,993 (GRCm39)	critical splice donor site	probably null
R6528:Mycbp2	UTSW	14	103,380,317 (GRCm39)	missense	probably damaging	0.99
R6736:Mycbp2	UTSW	14	103,429,003 (GRCm39)	missense	probably null	1.00
R6821:Mycbp2	UTSW	14	103,376,845 (GRCm39)	missense	probably damaging	1.00
R6851:Mycbp2	UTSW	14	103,497,630 (GRCm39)	critical splice donor site	probably null
R6948:Mycbp2	UTSW	14	103,522,703 (GRCm39)	missense	possibly damaging	0.94
R6977:Mycbp2	UTSW	14	103,392,342 (GRCm39)	missense	probably damaging	0.99
R6985:Mycbp2	UTSW	14	103,444,117 (GRCm39)	missense	possibly damaging	0.79
R7035:Mycbp2	UTSW	14	103,412,417 (GRCm39)	missense	probably benign
R7054:Mycbp2	UTSW	14	103,393,534 (GRCm39)	missense	possibly damaging	0.90
R7108:Mycbp2	UTSW	14	103,360,039 (GRCm39)	missense	probably damaging	1.00
R7117:Mycbp2	UTSW	14	103,391,513 (GRCm39)	missense	probably benign	0.21
R7137:Mycbp2	UTSW	14	103,520,115 (GRCm39)	missense	possibly damaging	0.94
R7169:Mycbp2	UTSW	14	103,497,636 (GRCm39)	missense	possibly damaging	0.78
R7218:Mycbp2	UTSW	14	103,371,282 (GRCm39)	missense	probably benign
R7234:Mycbp2	UTSW	14	103,452,773 (GRCm39)	missense	probably damaging	0.98
R7238:Mycbp2	UTSW	14	103,393,733 (GRCm39)	missense	probably damaging	1.00
R7244:Mycbp2	UTSW	14	103,446,345 (GRCm39)	missense	probably damaging	0.98
R7265:Mycbp2	UTSW	14	103,434,679 (GRCm39)	critical splice donor site	probably null
R7286:Mycbp2	UTSW	14	103,358,027 (GRCm39)	missense	probably damaging	1.00
R7332:Mycbp2	UTSW	14	103,434,793 (GRCm39)	missense	probably damaging	0.97
R7332:Mycbp2	UTSW	14	103,393,889 (GRCm39)	missense	probably damaging	1.00
R7384:Mycbp2	UTSW	14	103,513,829 (GRCm39)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,480,564 (GRCm39)	missense	probably damaging	0.99
R7392:Mycbp2	UTSW	14	103,389,627 (GRCm39)	missense	probably damaging	1.00
R7409:Mycbp2	UTSW	14	103,526,180 (GRCm39)	missense	probably damaging	1.00
R7486:Mycbp2	UTSW	14	103,434,690 (GRCm39)	missense	probably damaging	0.97
R7643:Mycbp2	UTSW	14	103,583,701 (GRCm39)	missense	probably benign
R7661:Mycbp2	UTSW	14	103,450,059 (GRCm39)	missense	probably damaging	1.00
R7663:Mycbp2	UTSW	14	103,429,045 (GRCm39)	missense	probably damaging	0.99
R7730:Mycbp2	UTSW	14	103,360,791 (GRCm39)	missense	probably damaging	0.99
R7757:Mycbp2	UTSW	14	103,429,055 (GRCm39)	missense	probably damaging	1.00
R7773:Mycbp2	UTSW	14	103,485,840 (GRCm39)	missense	probably damaging	0.97
R7787:Mycbp2	UTSW	14	103,364,533 (GRCm39)	missense	probably damaging	1.00
R7822:Mycbp2	UTSW	14	103,376,851 (GRCm39)	missense	probably benign	0.00
R7838:Mycbp2	UTSW	14	103,414,729 (GRCm39)	missense	probably benign	0.10
R7841:Mycbp2	UTSW	14	103,384,267 (GRCm39)	critical splice donor site	probably null
R7858:Mycbp2	UTSW	14	103,393,741 (GRCm39)	missense	probably damaging	1.00
R7873:Mycbp2	UTSW	14	103,393,582 (GRCm39)	missense	probably damaging	1.00
R7911:Mycbp2	UTSW	14	103,437,621 (GRCm39)	missense	probably damaging	0.99
R7942:Mycbp2	UTSW	14	103,392,674 (GRCm39)	missense	probably damaging	0.99
R7951:Mycbp2	UTSW	14	103,452,898 (GRCm39)	missense	probably damaging	0.99
R7958:Mycbp2	UTSW	14	103,367,400 (GRCm39)	missense	probably benign	0.00
R8235:Mycbp2	UTSW	14	103,436,110 (GRCm39)	missense	probably damaging	0.99
R8246:Mycbp2	UTSW	14	103,392,640 (GRCm39)	missense	probably damaging	0.99
R8338:Mycbp2	UTSW	14	103,372,701 (GRCm39)	missense	probably damaging	1.00
R8343:Mycbp2	UTSW	14	103,398,111 (GRCm39)	splice site	probably null
R8361:Mycbp2	UTSW	14	103,376,250 (GRCm39)	missense	probably damaging	1.00
R8490:Mycbp2	UTSW	14	103,446,267 (GRCm39)	missense	probably benign	0.00
R8524:Mycbp2	UTSW	14	103,392,895 (GRCm39)	missense	probably benign	0.23
R8525:Mycbp2	UTSW	14	103,450,155 (GRCm39)	missense	probably damaging	1.00
R8711:Mycbp2	UTSW	14	103,407,430 (GRCm39)	missense	probably benign	0.08
R8735:Mycbp2	UTSW	14	103,460,586 (GRCm39)	missense	probably damaging	0.99
R8825:Mycbp2	UTSW	14	103,466,871 (GRCm39)	missense	probably damaging	1.00
R8928:Mycbp2	UTSW	14	103,393,781 (GRCm39)	missense	probably benign
R8974:Mycbp2	UTSW	14	103,361,857 (GRCm39)	missense	probably damaging	1.00
R8987:Mycbp2	UTSW	14	103,446,232 (GRCm39)	missense	probably damaging	1.00
R9021:Mycbp2	UTSW	14	103,551,752 (GRCm39)	missense	probably benign	0.08
R9062:Mycbp2	UTSW	14	103,479,796 (GRCm39)	missense	probably benign	0.00
R9077:Mycbp2	UTSW	14	103,469,974 (GRCm39)	missense	probably damaging	1.00
R9208:Mycbp2	UTSW	14	103,532,664 (GRCm39)	missense	probably benign	0.01
R9285:Mycbp2	UTSW	14	103,434,753 (GRCm39)	missense	probably damaging	0.97
R9290:Mycbp2	UTSW	14	103,425,960 (GRCm39)	missense	probably damaging	0.99
R9362:Mycbp2	UTSW	14	103,497,642 (GRCm39)	missense	probably damaging	0.97
R9520:Mycbp2	UTSW	14	103,497,705 (GRCm39)	missense	probably benign	0.02
R9557:Mycbp2	UTSW	14	103,372,697 (GRCm39)	missense	probably benign	0.03
R9639:Mycbp2	UTSW	14	103,433,817 (GRCm39)	missense	probably damaging	1.00
R9666:Mycbp2	UTSW	14	103,371,474 (GRCm39)	missense	probably damaging	0.98
R9732:Mycbp2	UTSW	14	103,448,749 (GRCm39)	missense	probably damaging	1.00
R9736:Mycbp2	UTSW	14	103,434,852 (GRCm39)	missense	probably damaging	0.96
R9739:Mycbp2	UTSW	14	103,520,229 (GRCm39)	missense	probably benign	0.11
R9755:Mycbp2	UTSW	14	103,551,806 (GRCm39)	missense	probably benign
X0024:Mycbp2	UTSW	14	103,384,378 (GRCm39)	missense	probably damaging	1.00
Z1176:Mycbp2	UTSW	14	103,583,685 (GRCm39)	missense	probably benign
Z1176:Mycbp2	UTSW	14	103,394,073 (GRCm39)	missense	probably benign	0.06
Z1177:Mycbp2	UTSW	14	103,407,309 (GRCm39)	missense	possibly damaging	0.83
Z1177:Mycbp2	UTSW	14	103,372,559 (GRCm39)	missense	probably damaging	1.00
Z1177:Mycbp2	UTSW	14	103,364,499 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- ACAAGCCATTGCATCATGTAC -3'
(R):5'- TCTGAAAACAGAGCCCCTTCTC -3'

Sequencing Primer
(F):5'- CAAGCCATTGCATCATGTACTATGC -3'
(R):5'- CCTCCACAGTGAGGTAGTTGAAGTC -3'

Posted On

2014-10-01