Mutagenetix > Incidental Mutation

Incidental Mutation 'R2160:Lrrk2'

235131

Institutional Source

Beutler Lab

Gene Symbol

Lrrk2

Ensembl Gene

ENSMUSG00000036273

Gene Name

leucine-rich repeat kinase 2

Synonyms

9330188B09Rik, 4921513O20Rik, LOC381026, cI-46, D630001M17Rik

MMRRC Submission

040163-MU

Accession Numbers

Genbank: NM_025730; MGI: 1913975

Essential gene?

Possibly essential (E-score: 0.533) question?

Stock #

R2160 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

91673175-91816120 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 91796060 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Phenylalanine at position 2058 (S2058F)

Ref Sequence

ENSEMBL: ENSMUSP00000052584 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060642]

AlphaFold

Q5S006

Predicted Effect

probably damaging
Transcript: ENSMUST00000060642
AA Change: S2058F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000052584
Gene: ENSMUSG00000036273
AA Change: S2058F

Domain	Start	End	E-Value	Type
low complexity region	138	156	N/A	INTRINSIC
low complexity region	332	347	N/A	INTRINSIC
ANK	708	737	3.95e1	SMART
ANK	770	800	4.58e2	SMART
low complexity region	890	901	N/A	INTRINSIC
low complexity region	953	966	N/A	INTRINSIC
low complexity region	971	979	N/A	INTRINSIC
LRR	1010	1033	9.96e-1	SMART
LRR	1034	1057	8.01e0	SMART
LRR	1082	1105	2.45e0	SMART
LRR	1128	1151	9.3e-1	SMART
LRR	1195	1219	3.24e0	SMART
LRR	1244	1266	3.87e1	SMART
LRR	1267	1291	4.98e1	SMART
Pfam:Roc	1336	1456	4.9e-32	PFAM
Pfam:Ras	1336	1489	3.3e-17	PFAM
Pfam:COR	1524	1740	4e-28	PFAM
Pfam:Pkinase	1881	2132	4.7e-40	PFAM
Pfam:Pkinase_Tyr	1882	2132	6.8e-39	PFAM
WD40	2231	2276	3.09e-1	SMART
WD40	2401	2438	1.37e2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133743

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-in allele exhibit impaired response to dopamine, amphetamine, and quinpirole. Mice homozygous for one knock-out allele exhibit increased neurite growth. Mice homozygous for different knock-out alleles exhibit alopecia due to excessive grooming or kdiney atrophy. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, knock-out(6) Targeted, other(1)

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bpifb9b	A	G	2: 154,319,675	N576D	possibly damaging	Het
Braf	C	T	6: 39,662,073	C248Y	probably damaging	Het
Carmil2	A	G	8: 105,697,048	E1218G	possibly damaging	Het
Cntnap4	G	T	8: 112,757,571	G419C	probably damaging	Het
Csmd3	CCTTTGCGCTT	CCTT	15: 47,741,236		probably null	Het
Dnah9	C	T	11: 66,117,483	D839N	probably damaging	Het
Evc2	A	G	5: 37,380,518	T517A	possibly damaging	Het
Fbxw8	G	A	5: 118,124,988	P209S	probably damaging	Het
Gcm1	A	G	9: 78,061,380	K121E	probably benign	Het
Gprc6a	CAAA	CA	10: 51,615,680		probably null	Het
Herc2	A	G	7: 56,212,922	D4077G	probably benign	Het
Inpp4b	T	A	8: 82,121,375	L937*	probably null	Het
Ipcef1	A	T	10: 6,890,650	I349N	probably damaging	Het
Ipmk	A	G	10: 71,381,426	T267A	probably benign	Het
Jph3	G	T	8: 121,753,231	R216L	possibly damaging	Het
Kctd16	A	G	18: 40,259,085	E242G	probably damaging	Het
Klk14	G	A	7: 43,692,077	C51Y	probably damaging	Het
Krt76	A	G	15: 101,888,385	Y360H	probably damaging	Het
Lctl	A	G	9: 64,117,767	I12V	probably benign	Het
Lrig3	T	C	10: 125,997,696	V347A	possibly damaging	Het
Mark2	G	C	19: 7,282,747	S111C	probably damaging	Het
Mon2	T	A	10: 123,075,929	K12*	probably null	Het
Nupl1	A	G	14: 60,239,508	V238A	probably benign	Het
Pak7	T	C	2: 136,098,382	D504G	probably benign	Het
Pla2g4e	T	C	2: 120,185,206	S286G	probably benign	Het
Ppfia4	A	T	1: 134,313,723	V498D	probably benign	Het
Ppfibp1	A	G	6: 147,027,453	E846G	probably damaging	Het
Ppp3ca	G	A	3: 136,877,630	C166Y	probably damaging	Het
Prpf3	T	C	3: 95,845,230	K244E	probably benign	Het
Pzp	A	G	6: 128,525,276	S37P	probably damaging	Het
Rab11fip3	G	A	17: 26,069,054	H42Y	probably benign	Het
Sprn	C	A	7: 140,153,506		probably benign	Het
Tectb	C	G	19: 55,180,999		probably benign	Het
Thap12	A	G	7: 98,710,126	S71G	probably damaging	Het
Vmn1r7	A	G	6: 57,024,894	F127S	probably damaging	Het
Vmn2r54	C	T	7: 12,615,493	V721I	probably benign	Het
Vmn2r56	A	G	7: 12,694,219	F707L	probably benign	Het
Zfp976	C	T	7: 42,613,930	S161N	probably benign	Het

Other mutations in Lrrk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Lrrk2	APN	15	91747799	missense	possibly damaging	0.90
IGL00542:Lrrk2	APN	15	91699943	missense	probably benign
IGL00770:Lrrk2	APN	15	91801833	splice site	probably benign
IGL00774:Lrrk2	APN	15	91801833	splice site	probably benign
IGL00791:Lrrk2	APN	15	91779841	missense	probably damaging	1.00
IGL00827:Lrrk2	APN	15	91755790	missense	probably damaging	1.00
IGL00843:Lrrk2	APN	15	91757058	missense	possibly damaging	0.58
IGL01109:Lrrk2	APN	15	91738832	missense	probably damaging	1.00
IGL01293:Lrrk2	APN	15	91726137	missense	probably benign	0.21
IGL01296:Lrrk2	APN	15	91683142	missense	probably benign
IGL01301:Lrrk2	APN	15	91767339	missense	probably damaging	1.00
IGL01360:Lrrk2	APN	15	91700569	splice site	probably null
IGL01465:Lrrk2	APN	15	91728925	missense	probably benign	0.21
IGL01529:Lrrk2	APN	15	91812313	missense	possibly damaging	0.92
IGL01557:Lrrk2	APN	15	91699989	missense	probably damaging	1.00
IGL01560:Lrrk2	APN	15	91774988	missense	probably benign	0.33
IGL01991:Lrrk2	APN	15	91779946	missense	probably damaging	0.99
IGL02003:Lrrk2	APN	15	91731491	missense	probably damaging	0.99
IGL02325:Lrrk2	APN	15	91726308	critical splice donor site	probably null
IGL02711:Lrrk2	APN	15	91685822	missense	possibly damaging	0.71
IGL02869:Lrrk2	APN	15	91750277	missense	probably damaging	1.00
IGL03104:Lrrk2	APN	15	91747755	missense	possibly damaging	0.68
IGL03179:Lrrk2	APN	15	91700578	missense	probably damaging	1.00
IGL03395:Lrrk2	APN	15	91797414	splice site	probably null
horned	UTSW	15	91772858	missense	probably damaging	1.00
R1312_Lrrk2_980	UTSW	15	91699895	missense	probably damaging	1.00
R4710_lrrk2_232	UTSW	15	91699927	missense	possibly damaging	0.88
R5245_Lrrk2_127	UTSW	15	91796089	missense	probably damaging	1.00
spree	UTSW	15	91702247	missense	probably benign	0.00
Spur	UTSW	15	91774995	nonsense	probably null
3-1:Lrrk2	UTSW	15	91801934	missense	probably benign	0.01
ANU18:Lrrk2	UTSW	15	91767339	missense	probably damaging	1.00
H8562:Lrrk2	UTSW	15	91673358	missense	probably benign
H8786:Lrrk2	UTSW	15	91673358	missense	probably benign
IGL02835:Lrrk2	UTSW	15	91814660	critical splice acceptor site	probably null
R0014:Lrrk2	UTSW	15	91802045	splice site	probably benign
R0014:Lrrk2	UTSW	15	91802045	splice site	probably benign
R0078:Lrrk2	UTSW	15	91734009	missense	probably benign	0.01
R0100:Lrrk2	UTSW	15	91745796	missense	probably damaging	1.00
R0282:Lrrk2	UTSW	15	91778414	splice site	probably benign
R0448:Lrrk2	UTSW	15	91709305	missense	probably damaging	0.99
R0449:Lrrk2	UTSW	15	91750275	missense	probably damaging	1.00
R0610:Lrrk2	UTSW	15	91815416	missense	probably benign
R0617:Lrrk2	UTSW	15	91752278	missense	probably benign	0.00
R0632:Lrrk2	UTSW	15	91796028	missense	probably damaging	0.98
R0639:Lrrk2	UTSW	15	91772996	missense	probably benign	0.03
R0661:Lrrk2	UTSW	15	91787016	missense	probably damaging	1.00
R0666:Lrrk2	UTSW	15	91757070	critical splice donor site	probably null
R0764:Lrrk2	UTSW	15	91775046	splice site	probably null
R0766:Lrrk2	UTSW	15	91699895	missense	probably damaging	1.00
R0845:Lrrk2	UTSW	15	91755962	missense	probably benign	0.22
R0940:Lrrk2	UTSW	15	91729081	missense	possibly damaging	0.83
R0970:Lrrk2	UTSW	15	91729169	missense	probably benign	0.22
R1080:Lrrk2	UTSW	15	91673689	missense	probably benign	0.01
R1114:Lrrk2	UTSW	15	91700468	nonsense	probably null
R1223:Lrrk2	UTSW	15	91673635	missense	probably benign	0.00
R1289:Lrrk2	UTSW	15	91812360	missense	probably benign	0.00
R1296:Lrrk2	UTSW	15	91728920	missense	probably damaging	1.00
R1312:Lrrk2	UTSW	15	91699895	missense	probably damaging	1.00
R1637:Lrrk2	UTSW	15	91734058	missense	probably benign
R1773:Lrrk2	UTSW	15	91779981	missense	possibly damaging	0.96
R1809:Lrrk2	UTSW	15	91699892	missense	possibly damaging	0.86
R1839:Lrrk2	UTSW	15	91683134	missense	probably benign	0.00
R1946:Lrrk2	UTSW	15	91736661	splice site	probably null
R2232:Lrrk2	UTSW	15	91764716	missense	probably benign	0.05
R2419:Lrrk2	UTSW	15	91797526	splice site	probably benign
R2516:Lrrk2	UTSW	15	91755927	missense	probably benign
R3110:Lrrk2	UTSW	15	91814695	missense	probably benign	0.02
R3112:Lrrk2	UTSW	15	91814695	missense	probably benign	0.02
R3801:Lrrk2	UTSW	15	91737111	missense	probably benign
R3842:Lrrk2	UTSW	15	91755916	missense	probably benign	0.01
R3903:Lrrk2	UTSW	15	91747700	missense	probably damaging	1.00
R3903:Lrrk2	UTSW	15	91747701	missense	probably damaging	1.00
R3930:Lrrk2	UTSW	15	91767461	critical splice donor site	probably null
R3937:Lrrk2	UTSW	15	91778504	missense	probably damaging	0.98
R3938:Lrrk2	UTSW	15	91712780	missense	possibly damaging	0.69
R3938:Lrrk2	UTSW	15	91778504	missense	probably damaging	0.98
R3982:Lrrk2	UTSW	15	91709284	missense	probably benign	0.22
R4125:Lrrk2	UTSW	15	91815483	missense	probably benign	0.01
R4130:Lrrk2	UTSW	15	91755794	missense	probably benign	0.19
R4296:Lrrk2	UTSW	15	91699895	missense	probably damaging	1.00
R4465:Lrrk2	UTSW	15	91747820	missense	probably damaging	0.96
R4478:Lrrk2	UTSW	15	91723188	missense	probably damaging	1.00
R4517:Lrrk2	UTSW	15	91705120	missense	probably benign
R4539:Lrrk2	UTSW	15	91729142	missense	possibly damaging	0.86
R4654:Lrrk2	UTSW	15	91765681	missense	probably damaging	0.96
R4710:Lrrk2	UTSW	15	91699927	missense	possibly damaging	0.88
R4722:Lrrk2	UTSW	15	91688901	missense	probably damaging	1.00
R4723:Lrrk2	UTSW	15	91764759	missense	probably damaging	1.00
R4732:Lrrk2	UTSW	15	91688849	missense	probably damaging	1.00
R4732:Lrrk2	UTSW	15	91765747	missense	probably damaging	1.00
R4733:Lrrk2	UTSW	15	91688849	missense	probably damaging	1.00
R4733:Lrrk2	UTSW	15	91765747	missense	probably damaging	1.00
R4787:Lrrk2	UTSW	15	91712828	missense	probably benign
R4945:Lrrk2	UTSW	15	91804920	missense	probably benign	0.02
R4948:Lrrk2	UTSW	15	91803389	missense	probably benign	0.20
R5000:Lrrk2	UTSW	15	91749878	missense	probably damaging	1.00
R5031:Lrrk2	UTSW	15	91700619	missense	possibly damaging	0.50
R5067:Lrrk2	UTSW	15	91765790	missense	probably benign	0.01
R5245:Lrrk2	UTSW	15	91796089	missense	probably damaging	1.00
R5341:Lrrk2	UTSW	15	91772858	missense	probably damaging	1.00
R5460:Lrrk2	UTSW	15	91814644	splice site	probably null
R5551:Lrrk2	UTSW	15	91812350	missense	probably benign
R5574:Lrrk2	UTSW	15	91787016	missense	probably damaging	1.00
R5577:Lrrk2	UTSW	15	91765745	missense	probably damaging	1.00
R5685:Lrrk2	UTSW	15	91803301	nonsense	probably null
R5712:Lrrk2	UTSW	15	91702222	nonsense	probably null
R5728:Lrrk2	UTSW	15	91774974	missense	probably benign	0.36
R5782:Lrrk2	UTSW	15	91702183	missense	probably damaging	1.00
R5788:Lrrk2	UTSW	15	91764648	missense	possibly damaging	0.55
R5821:Lrrk2	UTSW	15	91709390	critical splice donor site	probably null
R5852:Lrrk2	UTSW	15	91755949	missense	probably damaging	1.00
R5934:Lrrk2	UTSW	15	91734046	missense	probably benign	0.00
R5935:Lrrk2	UTSW	15	91745831	missense	probably benign	0.14
R5979:Lrrk2	UTSW	15	91772945	missense	possibly damaging	0.47
R6101:Lrrk2	UTSW	15	91723135	missense	probably benign	0.10
R6114:Lrrk2	UTSW	15	91747826	missense	probably benign	0.33
R6259:Lrrk2	UTSW	15	91702247	missense	probably benign	0.00
R6376:Lrrk2	UTSW	15	91742266	missense	possibly damaging	0.89
R6417:Lrrk2	UTSW	15	91812346	missense	probably benign	0.03
R6420:Lrrk2	UTSW	15	91812346	missense	probably benign	0.03
R6737:Lrrk2	UTSW	15	91723218	missense	possibly damaging	0.50
R7056:Lrrk2	UTSW	15	91774995	nonsense	probably null
R7072:Lrrk2	UTSW	15	91801920	missense	probably benign	0.03
R7109:Lrrk2	UTSW	15	91764782	missense	probably damaging	1.00
R7128:Lrrk2	UTSW	15	91801885	missense	probably benign
R7144:Lrrk2	UTSW	15	91734055	missense	possibly damaging	0.54
R7187:Lrrk2	UTSW	15	91757001	missense	possibly damaging	0.92
R7270:Lrrk2	UTSW	15	91700441	missense	probably benign	0.01
R7356:Lrrk2	UTSW	15	91738744	missense	probably benign	0.07
R7360:Lrrk2	UTSW	15	91731655	critical splice donor site	probably null
R7373:Lrrk2	UTSW	15	91700004	critical splice donor site	probably null
R7465:Lrrk2	UTSW	15	91767340	missense	probably damaging	1.00
R7477:Lrrk2	UTSW	15	91812325	missense	probably damaging	0.98
R7614:Lrrk2	UTSW	15	91772858	missense	probably damaging	1.00
R7622:Lrrk2	UTSW	15	91812323	missense	probably damaging	1.00
R7658:Lrrk2	UTSW	15	91700358	missense	possibly damaging	0.91
R7679:Lrrk2	UTSW	15	91726186	missense	possibly damaging	0.58
R7737:Lrrk2	UTSW	15	91815446	missense	probably damaging	0.98
R7739:Lrrk2	UTSW	15	91700613	missense	probably damaging	1.00
R7740:Lrrk2	UTSW	15	91767324	missense	probably damaging	1.00
R7908:Lrrk2	UTSW	15	91726152	missense	probably damaging	1.00
R8299:Lrrk2	UTSW	15	91673240	start gained	probably benign
R8389:Lrrk2	UTSW	15	91699991	missense	probably damaging	1.00
R8462:Lrrk2	UTSW	15	91731477	missense	probably benign
R8698:Lrrk2	UTSW	15	91752197	missense	probably benign	0.38
R8947:Lrrk2	UTSW	15	91702270	nonsense	probably null
R9084:Lrrk2	UTSW	15	91750266	missense
R9086:Lrrk2	UTSW	15	91755848	missense	probably benign	0.01
R9096:Lrrk2	UTSW	15	91673256	start gained	probably benign
R9097:Lrrk2	UTSW	15	91673256	start gained	probably benign
R9267:Lrrk2	UTSW	15	91700426	missense	probably damaging	0.99
R9285:Lrrk2	UTSW	15	91778483	missense	probably damaging	1.00
R9341:Lrrk2	UTSW	15	91700415	missense	probably benign	0.18
R9343:Lrrk2	UTSW	15	91700415	missense	probably benign	0.18
R9371:Lrrk2	UTSW	15	91723204	missense	probably damaging	1.00
R9424:Lrrk2	UTSW	15	91752185	nonsense	probably null
R9489:Lrrk2	UTSW	15	91737217	missense	probably benign	0.37
R9502:Lrrk2	UTSW	15	91723162	missense	probably damaging	0.98
R9563:Lrrk2	UTSW	15	91749840	missense	possibly damaging	0.90
R9576:Lrrk2	UTSW	15	91752185	nonsense	probably null
R9605:Lrrk2	UTSW	15	91737217	missense	probably benign	0.37
R9635:Lrrk2	UTSW	15	91812324	missense	probably benign	0.21
R9641:Lrrk2	UTSW	15	91787048	missense	possibly damaging	0.94
R9660:Lrrk2	UTSW	15	91734025	missense	probably benign	0.00
R9673:Lrrk2	UTSW	15	91765681	missense	probably damaging	1.00
R9708:Lrrk2	UTSW	15	91750279	nonsense	probably null
R9728:Lrrk2	UTSW	15	91734025	missense	probably benign	0.00
R9757:Lrrk2	UTSW	15	91811026	missense	probably benign	0.03
RF001:Lrrk2	UTSW	15	91736633	missense	probably benign	0.11
X0028:Lrrk2	UTSW	15	91738851	missense	probably benign	0.00
Z1088:Lrrk2	UTSW	15	91726240	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GCTCACTGACTTCTTCCAGG -3'
(R):5'- TGCACTCAGCCAAAGTCATC -3'

Sequencing Primer
(F):5'- TTACTGCAGCAATGACCTGG -3'
(R):5'- TCAAACTCTACATGACCAAGCTG -3'

Posted On

2014-10-01