Incidental Mutation 'R2165:Mettl25b'
ID 235407
Institutional Source Beutler Lab
Gene Symbol Mettl25b
Ensembl Gene ENSMUSG00000004896
Gene Name methyltransferase like 25B
Synonyms BC023814, Rrnad1
MMRRC Submission 040168-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.077) question?
Stock # R2165 (G1)
Quality Score 178
Status Not validated
Chromosome 3
Chromosomal Location 87829908-87838024 bp(-) (GRCm39)
Type of Mutation critical splice donor site (2 bp from exon)
DNA Base Change (assembly) A to T at 87834360 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000124113 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000005016] [ENSMUST00000019854] [ENSMUST00000055984] [ENSMUST00000119968] [ENSMUST00000121048] [ENSMUST00000121920] [ENSMUST00000160074] [ENSMUST00000160074] [ENSMUST00000160143] [ENSMUST00000160143] [ENSMUST00000160648] [ENSMUST00000164439]
AlphaFold no structure available at present
Predicted Effect probably null
Transcript: ENSMUST00000005016
SMART Domains Protein: ENSMUSP00000005016
Gene: ENSMUSG00000004896

DomainStartEndE-ValueType
Pfam:Methyltransf_32 133 293 1.5e-29 PFAM
low complexity region 385 402 N/A INTRINSIC
low complexity region 412 426 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000019854
SMART Domains Protein: ENSMUSP00000019854
Gene: ENSMUSG00000019710

DomainStartEndE-ValueType
low complexity region 3 16 N/A INTRINSIC
KOW 55 82 7.17e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000055984
SMART Domains Protein: ENSMUSP00000059783
Gene: ENSMUSG00000048039

DomainStartEndE-ValueType
low complexity region 128 145 N/A INTRINSIC
low complexity region 157 168 N/A INTRINSIC
EXOIII 193 359 3.34e-38 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000119968
SMART Domains Protein: ENSMUSP00000114111
Gene: ENSMUSG00000019710

DomainStartEndE-ValueType
low complexity region 3 16 N/A INTRINSIC
KOW 55 82 7.17e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000121048
SMART Domains Protein: ENSMUSP00000113959
Gene: ENSMUSG00000019710

DomainStartEndE-ValueType
low complexity region 3 16 N/A INTRINSIC
KOW 55 82 7.17e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000121920
SMART Domains Protein: ENSMUSP00000112885
Gene: ENSMUSG00000019710

DomainStartEndE-ValueType
low complexity region 3 16 N/A INTRINSIC
KOW 55 82 7.17e-5 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159967
Predicted Effect probably null
Transcript: ENSMUST00000160074
SMART Domains Protein: ENSMUSP00000125365
Gene: ENSMUSG00000004896

DomainStartEndE-ValueType
Pfam:Methyltransf_32 69 229 1.3e-29 PFAM
low complexity region 321 338 N/A INTRINSIC
low complexity region 348 362 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000160074
SMART Domains Protein: ENSMUSP00000125365
Gene: ENSMUSG00000004896

DomainStartEndE-ValueType
Pfam:Methyltransf_32 69 229 1.3e-29 PFAM
low complexity region 321 338 N/A INTRINSIC
low complexity region 348 362 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000160143
SMART Domains Protein: ENSMUSP00000124113
Gene: ENSMUSG00000004896

DomainStartEndE-ValueType
Pfam:Methyltransf_32 133 247 5e-15 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000160143
SMART Domains Protein: ENSMUSP00000124113
Gene: ENSMUSG00000004896

DomainStartEndE-ValueType
Pfam:Methyltransf_32 133 247 5e-15 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000160648
Predicted Effect probably benign
Transcript: ENSMUST00000164439
Predicted Effect noncoding transcript
Transcript: ENSMUST00000168070
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159091
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159831
Predicted Effect noncoding transcript
Transcript: ENSMUST00000161471
Predicted Effect noncoding transcript
Transcript: ENSMUST00000160068
Predicted Effect noncoding transcript
Transcript: ENSMUST00000161014
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.0%
Validation Efficiency 99% (71/72)
Allele List at MGI
Other mutations in this stock
Total: 73 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca4 A G 3: 121,906,048 (GRCm39) T806A possibly damaging Het
Adgre1 T G 17: 57,726,338 (GRCm39) L403R probably damaging Het
Alox12 A T 11: 70,133,398 (GRCm39) probably null Het
Ankib1 A T 5: 3,763,210 (GRCm39) D506E possibly damaging Het
Ascc3 T G 10: 50,597,935 (GRCm39) Y1268D probably damaging Het
Bik A G 15: 83,425,624 (GRCm39) M42V probably benign Het
Bola1 A T 3: 96,104,517 (GRCm39) S26T probably benign Het
Bub1 T C 2: 127,643,201 (GRCm39) I1048V probably benign Het
Cad A G 5: 31,219,564 (GRCm39) N621S probably damaging Het
Camkv C A 9: 107,822,799 (GRCm39) N69K possibly damaging Het
Ccdc110 T C 8: 46,395,876 (GRCm39) M589T probably benign Het
Ccdc154 T A 17: 25,389,864 (GRCm39) V498E probably damaging Het
Ccr1l1 A G 9: 123,777,691 (GRCm39) L252P probably damaging Het
Cdh1 T C 8: 107,390,953 (GRCm39) C690R probably damaging Het
Cfap157 T G 2: 32,668,175 (GRCm39) probably null Het
Cux2 A G 5: 122,025,540 (GRCm39) S43P possibly damaging Het
Cyb5rl C T 4: 106,925,880 (GRCm39) P21S probably damaging Het
Cyp51 T G 5: 4,136,594 (GRCm39) Q400P probably damaging Het
Dnah7b T C 1: 46,137,152 (GRCm39) probably benign Het
Efcab3 G A 11: 104,642,688 (GRCm39) V1104I possibly damaging Het
Ephb4 A G 5: 137,352,688 (GRCm39) I90M probably benign Het
Fam13c A G 10: 70,378,523 (GRCm39) N269S probably damaging Het
Fam83c T A 2: 155,673,444 (GRCm39) Y248F possibly damaging Het
Fat2 A G 11: 55,194,542 (GRCm39) F1166L probably benign Het
Fem1a A G 17: 56,564,686 (GRCm39) N260D probably benign Het
Fnbp4 T A 2: 90,597,743 (GRCm39) probably null Het
Fut9 T A 4: 25,619,733 (GRCm39) *360Y probably null Het
Fut9 T A 4: 25,619,734 (GRCm39) *360L probably null Het
Gm3727 T A 14: 7,264,625 (GRCm38) Q10L probably damaging Het
Gpat2 G A 2: 127,270,211 (GRCm39) V75M probably damaging Het
Haspin A C 11: 73,027,456 (GRCm39) N544K probably damaging Het
Havcr1 A G 11: 46,669,379 (GRCm39) N286S probably benign Het
Lrp6 A C 6: 134,436,246 (GRCm39) C1307G probably damaging Het
Lrrc2 A C 9: 110,808,645 (GRCm39) H294P possibly damaging Het
Mon2 A C 10: 122,878,269 (GRCm39) probably null Het
Mrc2 G A 11: 105,239,257 (GRCm39) probably null Het
Mrgprb1 T C 7: 48,097,070 (GRCm39) I281V probably benign Het
Muc4 A G 16: 32,569,294 (GRCm39) E118G probably damaging Het
Nefh T C 11: 4,893,872 (GRCm39) D394G probably damaging Het
Neurl4 A G 11: 69,794,047 (GRCm39) T168A probably benign Het
Or10s1 A G 9: 39,986,211 (GRCm39) N207D possibly damaging Het
Or1j18 T A 2: 36,624,713 (GRCm39) C127S probably damaging Het
Or51a10 T C 7: 103,698,845 (GRCm39) T239A probably benign Het
Or5ak4 A T 2: 85,161,446 (GRCm39) N265K probably benign Het
Oxsr1 A C 9: 119,123,498 (GRCm39) M92R probably damaging Het
Pde8a T C 7: 80,945,516 (GRCm39) probably null Het
Pex5l T A 3: 33,007,281 (GRCm39) probably null Het
Pik3r4 T A 9: 105,549,984 (GRCm39) M1025K probably benign Het
Plch1 C T 3: 63,605,903 (GRCm39) E1325K probably benign Het
Plin2 A T 4: 86,586,669 (GRCm39) V54E probably damaging Het
Prss47 A T 13: 65,192,887 (GRCm39) I298N probably damaging Het
Prune2 A G 19: 17,097,546 (GRCm39) R1017G probably benign Het
Psg19 T A 7: 18,530,911 (GRCm39) Y81F possibly damaging Het
Ptch1 T G 13: 63,672,773 (GRCm39) E944A probably benign Het
Serpinb1a T C 13: 33,034,397 (GRCm39) probably benign Het
Sh3pxd2a A T 19: 47,266,794 (GRCm39) V265E probably damaging Het
Slc22a17 A T 14: 55,146,282 (GRCm39) Y337* probably null Het
Slc25a27 A G 17: 43,968,663 (GRCm39) V138A probably benign Het
Slc4a2 A G 5: 24,636,314 (GRCm39) Y220C probably damaging Het
Slc66a3 G A 12: 17,039,840 (GRCm39) L192F probably damaging Het
Stab1 A T 14: 30,890,392 (GRCm39) S20T probably benign Het
Tcstv2a A T 13: 120,725,633 (GRCm39) Q99L probably damaging Het
Thsd1 G T 8: 22,728,538 (GRCm39) probably benign Het
Tmem204 G A 17: 25,299,566 (GRCm39) probably benign Het
Tom1l1 A G 11: 90,540,721 (GRCm39) probably benign Het
Toporsl T A 4: 52,612,072 (GRCm39) F655Y possibly damaging Het
Vmn2r98 A G 17: 19,301,553 (GRCm39) K852E unknown Het
Wdpcp T G 11: 21,641,884 (GRCm39) L174R probably damaging Het
Zbbx G A 3: 75,019,414 (GRCm39) P99S probably damaging Het
Zfhx4 C T 3: 5,468,418 (GRCm39) P2859S probably benign Het
Zfp600 A T 4: 146,133,488 (GRCm39) R719* probably null Het
Zfp697 C A 3: 98,335,330 (GRCm39) A365E unknown Het
Zfp957 A C 14: 79,451,053 (GRCm39) S249A probably benign Het
Other mutations in Mettl25b
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1775:Mettl25b UTSW 3 87,831,124 (GRCm39) missense probably damaging 0.99
R2307:Mettl25b UTSW 3 87,834,162 (GRCm39) missense possibly damaging 0.88
R3957:Mettl25b UTSW 3 87,834,135 (GRCm39) missense possibly damaging 0.82
R4651:Mettl25b UTSW 3 87,834,979 (GRCm39) missense probably benign
R4663:Mettl25b UTSW 3 87,835,055 (GRCm39) missense probably damaging 1.00
R4818:Mettl25b UTSW 3 87,832,305 (GRCm39) missense probably benign 0.32
R5387:Mettl25b UTSW 3 87,837,318 (GRCm39) intron probably benign
R5427:Mettl25b UTSW 3 87,831,639 (GRCm39) unclassified probably benign
R7032:Mettl25b UTSW 3 87,831,649 (GRCm39) critical splice donor site probably null
R7048:Mettl25b UTSW 3 87,837,167 (GRCm39) missense probably damaging 1.00
R7953:Mettl25b UTSW 3 87,834,955 (GRCm39) missense possibly damaging 0.82
R8334:Mettl25b UTSW 3 87,835,056 (GRCm39) missense possibly damaging 0.46
R8969:Mettl25b UTSW 3 87,837,282 (GRCm39) intron probably benign
R9110:Mettl25b UTSW 3 87,834,978 (GRCm39) missense probably benign 0.03
R9142:Mettl25b UTSW 3 87,831,195 (GRCm39) missense probably benign 0.03
R9181:Mettl25b UTSW 3 87,835,392 (GRCm39) intron probably benign
R9257:Mettl25b UTSW 3 87,831,768 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- CATGAGGGAGGACAGCATTGTTC -3'
(R):5'- AGTCGAGGCTAGACACCTAC -3'

Sequencing Primer
(F):5'- AGGACAGCATTGTTCAGTGCC -3'
(R):5'- CCTCAGAACTGATTTGACATGTC -3'
Posted On 2014-10-01