Mutagenetix > Incidental Mutation

Incidental Mutation 'R2135:Or7e177'

235725

Institutional Source

Beutler Lab

Gene Symbol

Or7e177

Ensembl Gene

ENSMUSG00000049028

Gene Name

olfactory receptor family 7 subfamily E member 177

Synonyms

Olfr873, GA_x6K02T2PVTD-14040245-14041204, MOR145-2

MMRRC Submission

040138-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R2135 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20211507-20212466 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 20211593 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 32 (D32E)

Ref Sequence

ENSEMBL: ENSMUSP00000153816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053919] [ENSMUST00000075717] [ENSMUST00000215540]

AlphaFold

E9PX82

Predicted Effect

probably benign
Transcript: ENSMUST00000053919
AA Change: D29E

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000054778
Gene: ENSMUSG00000049028
AA Change: D29E

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	317	1.7e-52	PFAM
Pfam:7TM_GPCR_Srsx	45	315	1.6e-8	PFAM
Pfam:7tm_1	51	300	3e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000075717
AA Change: D33E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000075135
Gene: ENSMUSG00000049028
AA Change: D33E

Domain	Start	End	E-Value	Type
Pfam:7tm_4	45	321	6.2e-43	PFAM
Pfam:7TM_GPCR_Srsx	49	309	3e-8	PFAM
Pfam:7tm_1	55	304	1.5e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212393

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212793

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213057

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213445

Predicted Effect

probably benign
Transcript: ENSMUST00000215540
AA Change: D32E

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216567

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217193

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.6%

Validation Efficiency

100% (70/70)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,885,509 (GRCm39)	H116Q	probably benign	Het
Adamts16	C	A	13: 70,949,126 (GRCm39)	L178F	probably damaging	Het
Adgrv1	A	G	13: 81,672,676 (GRCm39)		probably null	Het
Akap9	A	G	5: 4,114,509 (GRCm39)	T46A	probably damaging	Het
Ankrd2	T	C	19: 42,032,498 (GRCm39)	L253P	probably damaging	Het
Arap3	T	C	18: 38,107,509 (GRCm39)	D1336G	probably damaging	Het
Cacna2d2	C	A	9: 107,403,712 (GRCm39)	L992I	possibly damaging	Het
Cdhr2	T	A	13: 54,868,760 (GRCm39)	I574N	probably damaging	Het
Cecr2	C	T	6: 120,697,923 (GRCm39)	T74M	probably damaging	Het
Ces5a	C	A	8: 94,226,369 (GRCm39)	E481D	probably benign	Het
Cfap74	C	G	4: 155,514,397 (GRCm39)	N544K	probably damaging	Het
Cfap74	T	A	4: 155,514,408 (GRCm39)	L548Q	probably damaging	Het
Cfb	C	T	17: 35,076,254 (GRCm39)	V1145I	possibly damaging	Het
CK137956	A	G	4: 127,845,433 (GRCm39)		probably benign	Het
Commd10	T	A	18: 47,123,604 (GRCm39)	L153Q	possibly damaging	Het
Cox6c	T	C	15: 35,937,429 (GRCm39)		probably benign	Het
Dock4	A	G	12: 40,795,667 (GRCm39)	Y828C	probably benign	Het
Ermp1	T	C	19: 29,623,465 (GRCm39)	D119G	possibly damaging	Het
Ext1	G	T	15: 52,965,140 (GRCm39)	Q409K	possibly damaging	Het
Fat4	A	T	3: 39,034,882 (GRCm39)	I2845F	probably damaging	Het
Frmd6	A	G	12: 70,941,771 (GRCm39)	N494S	probably benign	Het
Fscn3	A	G	6: 28,431,583 (GRCm39)	T305A	probably benign	Het
Gbx1	G	T	5: 24,731,220 (GRCm39)	R199S	possibly damaging	Het
Gm11437	T	C	11: 84,044,638 (GRCm39)	R192G	probably damaging	Het
Gucy2c	T	A	6: 136,700,726 (GRCm39)	D572V	probably damaging	Het
Idh1	A	C	1: 65,201,078 (GRCm39)	M291R	probably damaging	Het
Kcnt2	A	G	1: 140,356,551 (GRCm39)	Y330C	probably damaging	Het
Kdm4a	A	G	4: 117,999,656 (GRCm39)	L922P	probably damaging	Het
L3mbtl2	A	G	15: 81,566,215 (GRCm39)	D346G	possibly damaging	Het
Lsm14a	A	G	7: 34,070,609 (GRCm39)	S96P	probably damaging	Het
Ltn1	A	G	16: 87,179,601 (GRCm39)	L1520P	probably damaging	Het
Marchf6	A	T	15: 31,509,910 (GRCm39)	C26*	probably null	Het
Msra	G	A	14: 64,360,657 (GRCm39)	P228L	probably damaging	Het
Mycbp2	T	C	14: 103,446,329 (GRCm39)	Y1800C	probably damaging	Het
Mycbp2	A	T	14: 103,383,378 (GRCm39)	D395E	probably benign	Het
Ncr1	C	A	7: 4,343,756 (GRCm39)		probably benign	Het
Nkd1	T	C	8: 89,318,278 (GRCm39)	I201T	probably benign	Het
Nsrp1	A	T	11: 76,945,834 (GRCm39)		probably benign	Het
Or2t48	A	C	11: 58,420,611 (GRCm39)	I67S	probably damaging	Het
Pate5	A	C	9: 35,750,479 (GRCm39)		probably null	Het
Pkdrej	T	C	15: 85,700,707 (GRCm39)	Y1743C	probably damaging	Het
Plekha5	T	A	6: 140,526,225 (GRCm39)	H281Q	possibly damaging	Het
Ppp2r3d	A	C	9: 101,088,757 (GRCm39)	M522R	probably damaging	Het
Prdm12	T	C	2: 31,530,325 (GRCm39)	F72S	possibly damaging	Het
Prima1	A	T	12: 103,168,949 (GRCm39)	F106L	probably damaging	Het
Ptchd4	A	G	17: 42,627,965 (GRCm39)	H142R	probably benign	Het
Rasgrf2	G	A	13: 92,120,374 (GRCm39)	A793V	probably benign	Het
Rbsn	T	A	6: 92,166,854 (GRCm39)	M597L	probably benign	Het
Ripk4	A	T	16: 97,544,933 (GRCm39)	D571E	probably damaging	Het
Rp9	T	C	9: 22,379,425 (GRCm39)	K25E	possibly damaging	Het
Sec31b	T	C	19: 44,523,135 (GRCm39)	S211G	probably damaging	Het
Shank2	C	T	7: 143,964,971 (GRCm39)	P1070S	probably damaging	Het
Sla	A	G	15: 66,654,563 (GRCm39)	V241A	probably benign	Het
Slc25a40	A	G	5: 8,477,489 (GRCm39)	T25A	possibly damaging	Het
Slc3a2	A	G	19: 8,685,608 (GRCm39)	S164P	probably benign	Het
Slc7a5	A	T	8: 122,610,444 (GRCm39)	W457R	probably null	Het
Slco6c1	A	G	1: 97,032,542 (GRCm39)	M303T	probably benign	Het
Smim10l1	T	C	6: 133,082,489 (GRCm39)	F12S	probably damaging	Het
Syne2	A	G	12: 75,999,560 (GRCm39)	I2318V	probably damaging	Het
Tecta	A	C	9: 42,251,581 (GRCm39)	C1781G	probably damaging	Het
Timmdc1	A	T	16: 38,319,313 (GRCm39)	H280Q	probably benign	Het
Tktl2	G	A	8: 66,964,999 (GRCm39)	V186M	probably damaging	Het
Tmem30b	A	G	12: 73,592,107 (GRCm39)	L336P	probably damaging	Het
Tmem94	A	G	11: 115,685,575 (GRCm39)	I943V	probably benign	Het
Tshb	A	G	3: 102,685,464 (GRCm39)		probably null	Het
Vcpip1	A	G	1: 9,818,035 (GRCm39)	V116A	probably benign	Het
Vmn2r76	A	G	7: 85,880,219 (GRCm39)	Y156H	probably benign	Het
Zcrb1	T	C	15: 93,295,067 (GRCm39)	N23S	probably damaging	Het
Zfyve26	T	C	12: 79,292,826 (GRCm39)	R2108G	possibly damaging	Het

Other mutations in Or7e177

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02122:Or7e177	APN	9	20,211,880 (GRCm39)	missense	probably damaging	1.00
IGL02268:Or7e177	APN	9	20,211,588 (GRCm39)	missense	probably damaging	1.00
IGL02416:Or7e177	APN	9	20,211,541 (GRCm39)	missense	probably benign	0.01
IGL03124:Or7e177	APN	9	20,212,459 (GRCm39)	missense	probably benign	0.00
R0147:Or7e177	UTSW	9	20,212,387 (GRCm39)	missense	probably damaging	1.00
R0148:Or7e177	UTSW	9	20,212,387 (GRCm39)	missense	probably damaging	1.00
R0266:Or7e177	UTSW	9	20,212,454 (GRCm39)	missense	probably benign	0.01
R0831:Or7e177	UTSW	9	20,211,861 (GRCm39)	missense	probably benign	0.20
R1456:Or7e177	UTSW	9	20,212,134 (GRCm39)	missense	probably benign	0.35
R1894:Or7e177	UTSW	9	20,211,633 (GRCm39)	missense	probably benign	0.23
R1928:Or7e177	UTSW	9	20,212,354 (GRCm39)	missense	probably benign	0.12
R2379:Or7e177	UTSW	9	20,211,963 (GRCm39)	missense	possibly damaging	0.87
R2911:Or7e177	UTSW	9	20,211,775 (GRCm39)	missense	possibly damaging	0.60
R3788:Or7e177	UTSW	9	20,211,666 (GRCm39)	missense	probably benign	0.13
R4657:Or7e177	UTSW	9	20,211,919 (GRCm39)	missense	probably damaging	1.00
R5754:Or7e177	UTSW	9	20,212,390 (GRCm39)	missense	probably damaging	1.00
R6291:Or7e177	UTSW	9	20,211,899 (GRCm39)	missense	probably damaging	1.00
R6410:Or7e177	UTSW	9	20,211,748 (GRCm39)	missense	probably damaging	1.00
R7014:Or7e177	UTSW	9	20,211,959 (GRCm39)	nonsense	probably null
R7521:Or7e177	UTSW	9	20,212,036 (GRCm39)	missense	probably benign	0.00
R8201:Or7e177	UTSW	9	20,212,317 (GRCm39)	missense	probably damaging	1.00
R8355:Or7e177	UTSW	9	20,211,715 (GRCm39)	missense	probably damaging	1.00
R8455:Or7e177	UTSW	9	20,211,715 (GRCm39)	missense	probably damaging	1.00
R8523:Or7e177	UTSW	9	20,212,093 (GRCm39)	missense	probably benign	0.10
R8874:Or7e177	UTSW	9	20,212,069 (GRCm39)	missense	possibly damaging	0.95
R9283:Or7e177	UTSW	9	20,212,419 (GRCm39)	missense	possibly damaging	0.61
R9397:Or7e177	UTSW	9	20,211,748 (GRCm39)	missense	possibly damaging	0.50
R9595:Or7e177	UTSW	9	20,211,661 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCATAACTGAGATGGGGATTGATG -3'
(R):5'- CACAATCATCTTTGGGACTGTGG -3'

Sequencing Primer
(F):5'- TGCAGAAGATCAGTAATTTTGCC -3'
(R):5'- CTTTGGGACTGTGGTAGAGATAAAAC -3'

Posted On

2014-10-01