Mutagenetix > Incidental Mutation

Incidental Mutation 'R2136:Olfr142'

235786

Institutional Source

Beutler Lab

Gene Symbol

Olfr142

Ensembl Gene

ENSMUSG00000075063

Gene Name

olfactory receptor 142

Synonyms

GA_x6K02T2Q125-51607674-51606757, K20, MOR227-2

MMRRC Submission

040139-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R2136 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

90250130-90257592 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 90252253 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 245 (V245G)

Ref Sequence

ENSEMBL: ENSMUSP00000150216 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099752] [ENSMUST00000213968]

AlphaFold

Q60881

Predicted Effect

probably damaging
Transcript: ENSMUST00000099752
AA Change: V245G

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000097341
Gene: ENSMUSG00000075063
AA Change: V245G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	4.3e-53	PFAM
Pfam:7TM_GPCR_Srsx	33	300	3e-6	PFAM
Pfam:7tm_1	39	285	5.8e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213968
AA Change: V245G

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210010C04Rik	A	G	6: 41,035,462 (GRCm38)	F6S	probably benign	Het
2810474O19Rik	T	A	6: 149,328,822 (GRCm38)	I1122K	probably benign	Het
A930003A15Rik	T	G	16: 19,883,780 (GRCm38)		noncoding transcript	Het
Abca5	T	C	11: 110,319,832 (GRCm38)	T174A	probably benign	Het
Abcg3	G	A	5: 104,966,814 (GRCm38)	S279L	probably benign	Het
Acap3	T	C	4: 155,896,912 (GRCm38)	L85P	probably damaging	Het
Adgrl3	A	G	5: 81,512,254 (GRCm38)	K290R	probably damaging	Het
Ankhd1	A	G	18: 36,647,621 (GRCm38)	T1909A	probably benign	Het
Asap1	T	C	15: 64,110,959 (GRCm38)	D832G	probably damaging	Het
Atp6v0a2	T	A	5: 124,718,488 (GRCm38)	L702Q	possibly damaging	Het
Bsn	A	G	9: 108,113,231 (GRCm38)	V1774A	probably damaging	Het
Cd209e	T	C	8: 3,853,248 (GRCm38)	E48G	probably benign	Het
Cdadc1	T	C	14: 59,568,044 (GRCm38)		probably null	Het
Cfap65	C	CA	1: 74,917,273 (GRCm38)		probably null	Het
Cln3	A	C	7: 126,582,799 (GRCm38)	S30R	probably benign	Het
Cluap1	C	T	16: 3,933,772 (GRCm38)	R332W	probably damaging	Het
Crb1	A	T	1: 139,337,425 (GRCm38)	V85E	probably benign	Het
Crocc	G	A	4: 141,032,954 (GRCm38)	R789W	probably damaging	Het
Cwh43	A	G	5: 73,415,054 (GRCm38)	I212V	probably benign	Het
Cyp2t4	C	A	7: 27,158,160 (GRCm38)	F391L	probably benign	Het
Dhx35	G	T	2: 158,831,861 (GRCm38)	R404L	probably damaging	Het
Disp1	A	G	1: 183,088,378 (GRCm38)	L826S	probably damaging	Het
Dync2h1	T	A	9: 7,122,772 (GRCm38)	E2061D	probably damaging	Het
Ep300	T	A	15: 81,640,447 (GRCm38)	Y1393N	unknown	Het
Fap	C	T	2: 62,524,207 (GRCm38)	G446D	possibly damaging	Het
Fat3	A	G	9: 16,377,051 (GRCm38)	I392T	probably benign	Het
Fpr-rs4	CAGGAA	CA	17: 18,022,334 (GRCm38)		probably null	Het
Glipr1l1	T	C	10: 112,060,476 (GRCm38)	V56A	probably damaging	Het
Grsf1	A	G	5: 88,672,658 (GRCm38)	V7A	probably benign	Het
Hmcn1	G	A	1: 150,633,659 (GRCm38)	A3646V	probably damaging	Het
Ipo9	A	T	1: 135,394,285 (GRCm38)	I569N	probably damaging	Het
Irs1	G	T	1: 82,290,042 (GRCm38)	P151Q	probably damaging	Het
Kalrn	T	C	16: 34,307,724 (GRCm38)	D491G	possibly damaging	Het
Kctd7	T	C	5: 130,152,366 (GRCm38)	L210P	probably damaging	Het
Lifr	C	A	15: 7,181,857 (GRCm38)	D625E	possibly damaging	Het
Lrguk	T	C	6: 34,043,519 (GRCm38)	V201A	probably benign	Het
Mark1	A	G	1: 184,919,573 (GRCm38)	V135A	probably damaging	Het
Mical2	T	A	7: 112,271,515 (GRCm38)	D70E	possibly damaging	Het
Mrc1	T	C	2: 14,270,189 (GRCm38)	Y434H	probably damaging	Het
Myh10	C	A	11: 68,804,714 (GRCm38)	Q1556K	probably damaging	Het
Nav1	G	A	1: 135,454,436 (GRCm38)	T1400I	probably null	Het
Olfr1173	T	C	2: 88,274,240 (GRCm38)	K270E	probably damaging	Het
Olfr1178	T	A	2: 88,391,319 (GRCm38)	I24N	probably benign	Het
Olfr1289	T	C	2: 111,483,616 (GRCm38)	V62A	probably damaging	Het
Olfr13	A	G	6: 43,174,501 (GRCm38)	K172E	probably benign	Het
Olfr339	A	G	2: 36,421,938 (GRCm38)	D180G	probably damaging	Het
Olfr513	G	A	7: 108,755,223 (GRCm38)	M122I	possibly damaging	Het
Osmr	T	A	15: 6,852,462 (GRCm38)	Q67L	probably damaging	Het
Pan2	T	G	10: 128,313,637 (GRCm38)	V522G	possibly damaging	Het
Pard3	T	G	8: 127,376,885 (GRCm38)		probably null	Het
Pcdhgc5	G	T	18: 37,820,113 (GRCm38)	A147S	possibly damaging	Het
Pcsk9	T	C	4: 106,446,770 (GRCm38)	I506V	probably benign	Het
Polr3d	GCCCCC	GCCCC	14: 70,443,047 (GRCm38)		probably null	Het
Prdm4	G	A	10: 85,893,351 (GRCm38)	R731*	probably null	Het
Prdx6b	T	A	2: 80,293,163 (GRCm38)	D105E	probably damaging	Het
Rab42	A	G	4: 132,302,479 (GRCm38)	L144P	probably damaging	Het
Ralbp1	T	A	17: 65,864,666 (GRCm38)	K104M	probably damaging	Het
Rrp12	A	G	19: 41,892,599 (GRCm38)	V131A	probably damaging	Het
Sbno1	C	T	5: 124,387,534 (GRCm38)		probably null	Het
Sbno2	A	T	10: 80,062,693 (GRCm38)	I645N	probably damaging	Het
Scfd2	T	C	5: 74,206,367 (GRCm38)	K624R	probably benign	Het
Sgk2	C	A	2: 162,999,179 (GRCm38)		probably null	Het
Sirt4	A	G	5: 115,479,701 (GRCm38)	S299P	probably benign	Het
Slit2	C	T	5: 48,304,225 (GRCm38)	A1521V	probably benign	Het
Socs7	T	G	11: 97,373,107 (GRCm38)	V275G	possibly damaging	Het
Spink11	G	A	18: 44,190,487 (GRCm38)	P102S	probably benign	Het
Tacc3	A	G	5: 33,671,404 (GRCm38)	N534D	probably damaging	Het
Tas2r115	T	C	6: 132,737,346 (GRCm38)	Y214C	probably damaging	Het
Tcaf1	A	T	6: 42,673,520 (GRCm38)	M875K	probably benign	Het
Ttc22	T	A	4: 106,622,672 (GRCm38)	L41Q	possibly damaging	Het
Ttc37	T	C	13: 76,173,354 (GRCm38)	S1322P	possibly damaging	Het
Vasn	T	A	16: 4,649,795 (GRCm38)	C535*	probably null	Het
Vcan	T	A	13: 89,689,737 (GRCm38)	I2563F	probably damaging	Het
Vmn2r63	T	G	7: 42,926,873 (GRCm38)	Q505H	probably damaging	Het
Vmn2r65	T	G	7: 84,943,573 (GRCm38)	Q475H	probably damaging	Het
Zbtb43	T	C	2: 33,454,520 (GRCm38)	Y231C	probably damaging	Het
Zfp292	A	G	4: 34,810,266 (GRCm38)	V931A	probably benign	Het

Other mutations in Olfr142

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Olfr142	APN	2	90,252,609 (GRCm38)	missense	probably damaging	1.00
IGL01623:Olfr142	APN	2	90,252,609 (GRCm38)	missense	probably damaging	1.00
IGL01810:Olfr142	APN	2	90,252,132 (GRCm38)	nonsense	probably null
IGL01918:Olfr142	APN	2	90,252,331 (GRCm38)	missense	probably damaging	1.00
IGL02619:Olfr142	APN	2	90,252,505 (GRCm38)	missense	probably damaging	0.97
IGL02732:Olfr142	APN	2	90,252,308 (GRCm38)	missense	probably damaging	1.00
IGL02738:Olfr142	APN	2	90,252,355 (GRCm38)	missense	possibly damaging	0.82
IGL02795:Olfr142	APN	2	90,252,562 (GRCm38)	missense	probably damaging	1.00
IGL02830:Olfr142	APN	2	90,252,781 (GRCm38)	missense	probably damaging	1.00
R0601:Olfr142	UTSW	2	90,252,934 (GRCm38)	missense	probably benign	0.05
R2004:Olfr142	UTSW	2	90,252,692 (GRCm38)	missense	probably benign	0.04
R2377:Olfr142	UTSW	2	90,252,911 (GRCm38)	missense	probably damaging	1.00
R3615:Olfr142	UTSW	2	90,252,409 (GRCm38)	missense	possibly damaging	0.94
R3616:Olfr142	UTSW	2	90,252,409 (GRCm38)	missense	possibly damaging	0.94
R3777:Olfr142	UTSW	2	90,252,625 (GRCm38)	missense	probably damaging	1.00
R4763:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R4765:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R5421:Olfr142	UTSW	2	90,252,745 (GRCm38)	missense	probably benign	0.01
R5426:Olfr142	UTSW	2	90,252,611 (GRCm38)	nonsense	probably null
R6063:Olfr142	UTSW	2	90,252,427 (GRCm38)	missense	probably benign	0.40
R6717:Olfr142	UTSW	2	90,252,524 (GRCm38)	missense	probably benign	0.00
R6931:Olfr142	UTSW	2	90,252,777 (GRCm38)	nonsense	probably null
R6936:Olfr142	UTSW	2	90,252,334 (GRCm38)	missense	probably benign	0.17
R7013:Olfr142	UTSW	2	90,252,097 (GRCm38)	missense	possibly damaging	0.87
R7091:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R7247:Olfr142	UTSW	2	90,252,821 (GRCm38)	missense	probably damaging	1.00
R8169:Olfr142	UTSW	2	90,252,098 (GRCm38)	nonsense	probably null
R8345:Olfr142	UTSW	2	90,252,217 (GRCm38)	missense	possibly damaging	0.50
R9222:Olfr142	UTSW	2	90,252,476 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- GCTTCCAGGACATTCGTATAGC -3'
(R):5'- GTGGTCCCAATGTGATTGACC -3'

Sequencing Primer
(F):5'- CCAGGACATTCGTATAGCATTTTTC -3'
(R):5'- GGTCCCAATGTGATTGACCACTAC -3'

Posted On

2014-10-01